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SG11201811329UA - Spheroids including biologically-relevant materials and related methods - Google Patents

Spheroids including biologically-relevant materials and related methods

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Publication number
SG11201811329UA
SG11201811329UA SG11201811329UA SG11201811329UA SG11201811329UA SG 11201811329U A SG11201811329U A SG 11201811329UA SG 11201811329U A SG11201811329U A SG 11201811329UA SG 11201811329U A SG11201811329U A SG 11201811329UA SG 11201811329U A SG11201811329U A SG 11201811329UA
Authority
SG
Singapore
Prior art keywords
louisville
kentucky
suite
international
university
Prior art date
Application number
SG11201811329UA
Inventor
Stuart K Williams
Brian C Gettler
Joseph S Zakhari
Piyani S Gandhi
Original Assignee
University Of Louisville Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Louisville Research Foundation Inc filed Critical University Of Louisville Research Foundation Inc
Publication of SG11201811329UA publication Critical patent/SG11201811329UA/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Rheumatology (AREA)
  • Reproductive Health (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#11011110111010101111101 110100111101111110011101110 111111111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/005477 Al 04 January 2018 (04.01.2018) W I PO I PCT (51) International Patent Classification: search Foundation, Inc., 300 East Market Street, Suite 300, A61L 27/52 (2006.01) C08J 3/24 (2006.01) Louisville, Kentucky 40202 (US). A61L 27/54 (2006.01) A61K 35/12 (2015.01) (74) Agent: WRIGHT, Terry L. et al.; c/o Stites & Harbi- A61L 27/56 (2006.01) A61K 35/39 (2015.01) A61L 27/24 (2006.01) C12N 5/0735 (2010.01) son, PLLC, 400 West Market Street, Suite 1800, Louisville, Kentucky 40202 (US). (21) International Application Number: PCT/US2017/039483 (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 27 June 2017 (27.06.2017) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (25) Filing Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (26) Publication Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (30) Priority Data: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 62/354,929 27 June 2016 (27.06.2016) US OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 62/422,694 16 November 2016 (16.11.2016) US SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: UNIVERSITY OF LOUISVILLE (84) Designated States (unless otherwise indicated, for every RESEARCH FOUNDATION, INC. [US/US]; 300 East kind of regional protection available): ARIPO (BW, GH, Market Street, Suite 300, Louisville, Kentucky 40202-1959 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (US). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Inventors: WILLIAMS, Stuart K.; c/o University of TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Louisville Research Foundation, Inc., 300 East Market EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Street, Suite 300, Louisville, Kentucky 40202 (US). GET- MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, = (72) = — TLER, Brian C.; c/o University of Louisville Research TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Foundation, Inc., 300 East Market Street, Suite 300, KM, ML, MR, NE, SN, TD, TG). = Louisville, Kentucky 40202 (US). ZAKHARI, Joseph S.; c/o University of Louisville Research Foundation, Inc., 300 = East Market Street, Suite 300, Louisville, Kentucky 40202 _= = (US). GANDHI, Piyani S.; c/o University of Louisville Re- = Title: SPHEROIDS INCLUDING BIOLOGICALLY-RELEVANT MATERIALS AND RELATED METHODS = (54) = = Spheroid = = = = Pluronic = = Super Hydrophobic .4 \ , S U rf ac e IN IN 7r k r) FIG. ID 0 0 . a ' — c . (57) : A method of making a spheroid is provided that includes the step of providing a suspension having one or more bio- logically-relevant materials dispersed within a biocompatible medium. An amount of a hydrophilic material is deposited on a defined 0 area of a super-hydrophobic surface, and a droplet of the suspension is bioprinted onto the hydrophilic material positioned on the su- ei per-hydrophobic to thereby create the spheroid. [Continued on next page] WO 2018/005477 Al OII Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) — as to the applicant's entitlement to claim the earlier application (Rule 4.17(iii)) priority of the Published: — with international search report (Art. 21(3))
SG11201811329UA 2016-06-27 2017-06-27 Spheroids including biologically-relevant materials and related methods SG11201811329UA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662354929P 2016-06-27 2016-06-27
US201662422694P 2016-11-16 2016-11-16
PCT/US2017/039483 WO2018005477A1 (en) 2016-06-27 2017-06-27 Spheroids including biologically-relevant materials and related methods

Publications (1)

Publication Number Publication Date
SG11201811329UA true SG11201811329UA (en) 2019-01-30

Family

ID=60785530

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201811329UA SG11201811329UA (en) 2016-06-27 2017-06-27 Spheroids including biologically-relevant materials and related methods

Country Status (9)

Country Link
US (1) US11419962B2 (en)
EP (1) EP3474914A4 (en)
JP (1) JP6875670B2 (en)
KR (1) KR102446764B1 (en)
CN (1) CN109906092A (en)
AU (1) AU2017290569B2 (en)
CA (1) CA3028736A1 (en)
SG (1) SG11201811329UA (en)
WO (1) WO2018005477A1 (en)

Families Citing this family (9)

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US20230023276A1 (en) * 2016-06-27 2023-01-26 University Of Louisville Research Foundation, Inc. 3-d bioprinting comprising biologically-relevant materials and related methods
CN108715833B (en) * 2018-06-01 2021-09-14 天晴干细胞股份有限公司 Preparation method of microsphere loaded with platelet lysate
KR102489607B1 (en) * 2018-10-24 2023-01-17 포항공과대학교 산학협력단 Method of manufacturing cell spheroid using three-dimensional printing method
CN109569344B (en) * 2018-12-17 2021-01-08 浙江大学 Method for preparing suspended micro-droplets by using micro-fluidic device
KR102156310B1 (en) * 2019-03-06 2020-09-15 울산과학기술원 A method for manufacturing cell spheroid using bio-ink
CN110004111B (en) * 2019-04-22 2021-10-08 清华-伯克利深圳学院筹备办公室 A kind of preparation method of organoid spheroid
KR102201546B1 (en) * 2020-03-27 2021-01-11 가톨릭대학교 산학협력단 Acellular dermal matrix layer for living transplant loaded with cells and method for preparing same
EP4554794A1 (en) * 2022-07-12 2025-05-21 University of Louisville Research Foundation, Inc. 3-d bioprinting comprising biologically-relevant materials and related methods
CN115518193A (en) * 2022-09-23 2022-12-27 青岛大学 Fish skin collagen/SVF photo-crosslinking composite hydrogel and preparation method and application thereof

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Also Published As

Publication number Publication date
AU2017290569B2 (en) 2022-01-20
EP3474914A1 (en) 2019-05-01
US20190381213A1 (en) 2019-12-19
AU2017290569A1 (en) 2019-01-17
JP2019520824A (en) 2019-07-25
KR20190022790A (en) 2019-03-06
CA3028736A1 (en) 2018-01-04
JP6875670B2 (en) 2021-05-26
KR102446764B1 (en) 2022-09-22
CN109906092A (en) 2019-06-18
EP3474914A4 (en) 2020-01-15
WO2018005477A1 (en) 2018-01-04
US11419962B2 (en) 2022-08-23

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