SU395087A1 - Method for producing biologically active polyphenols from propolis - Google Patents

Description

one

The invention relates to the field of medicine.

A method of processing bee glue is known, according to which crushed propolis is extracted with ethanol, centrifuged, the solvent is distilled off, the remainder of the epitrack p: ropolis is obtained - a mixture of resins, balsams, and essential oils, left after the center of the fugirya, the mass is dried, extracted with carbon tetrachloride, obtained in a tray. Dry product - propolis wax. However, this method has a complex technological process, and the resulting product has a high enough quality.

The proposed method differs in that the mp1 process is subsequently repeatedly treated with petroleum ether, chloroform, and ethanol.

Example. 100 g of freshly assembled propoln (bee glue) are ground into a propeller | Noah mill to sieve No. 6, sieved and placed in a two-liter 1K01 ary. Flask with a fresh reflux water cooler, poured with petroleum ether in a ratio of 1: 10 to heat 1 kit. water bath for 1 hour. A similar cleaning is repeated 5 times. Further purification from waxes, LIPOIDES, resinous and other ballast materials; the CBBs are carried out by treating the polyol with chloroform in a boiling water bath in a 1: 5 ratio in a flask with a ground reflux condenser. A similar cleaning operation is carried out until there is no residue after evaporation of the solvent (6 times) and the nronolis purified in this way is dried until the chloroform is completely removed in a drying cabinet at 30-40 ° C.

In the treatment of propatis with both petrols: niter ether, and chloroform, acting biologically; active substances are not extracted (barely noticeable traces), they are monitored by the paper-chromatographic method in solvent systems: 15 / o-for acetic acid, and-butanol-acetic acid and water (4: 1: 2; 4: 1: five).

Then, the sum of biologically active active substances is extracted by absolutized synthetic ethanol (1: 5) until exhaustive extraction of the last one: x (15 times) in the same LHOL with a reflux condenser on a water bath. For the extraction of the active substances, the control is also carried out by paper-chromatographic method in the above solvent systems using aluminum chloride, zirconium nitrate and, in combination) with ammonia vapors and alkali as propellants.

The combined alcohol extracts are filtered, freed from mechanical impurities and evaporated under Baixyy.MOi.M Lr.z 40-50 ° C to a volume of about 200 l / l; one stripped off extract is transferred to a cooler with a temperature of i; lnus 15-20 ° C. At the same time, the total amount of active biologically active substances in the form of amorphous yellow-brown, powder, ka, which is filtered and dried in a desiccator, is precipitated; sulphate of sodium - in leaking 4 hours. The output of the drug.

11th day of invention

The method of obtaining biologically astive polkphenol from propolis by treating it with organic solvents followed by drying, characterized in that, in order to improve the quality of the target product and simplify the process, propolis is treated with petroleum ether, chloroform, ethanol.