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US6008372A - Substituted dinaphthylmethyl and diheteroarylmethylacetyl histidine inhibitors of protein farnesyltransferase - Google Patents

Substituted dinaphthylmethyl and diheteroarylmethylacetyl histidine inhibitors of protein farnesyltransferase Download PDF

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US6008372A
US6008372A US09/101,206 US10120698A US6008372A US 6008372 A US6008372 A US 6008372A US 10120698 A US10120698 A US 10120698A US 6008372 A US6008372 A US 6008372A
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naphthalen
imidazol
ylmethyl
hydrogen
propionylamino
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US09/101,206
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Annette M. Doherty
James S. Kaltenbronn
Daniele Leonard
John Quin, III
Jeffrey D. Scholten
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Warner Lambert Co LLC
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Warner Lambert Co LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
    • C07D409/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings

Definitions

  • the present invention relates to novel substituted histidine compounds useful as pharmaceutical agents, to methods for their production, to pharmaceutical compositions which include these compounds and a pharmaceutically acceptable carrier, and to pharmaceutical methods of treatment.
  • the novel compounds of the present invention inhibit farnesyl transferase enzyme which activates ras proteins which in turn activate cellular division. More particularly, the novel compounds of the present invention are useful in the treatment of proliferative diseases such as, for example, cancer, restenosis, and psoriasis, and as antiviral agents.
  • Ras protein (or p21) has been examined extensively because mutant forms are found in 20% of most types of human cancer and greater than 50% of colon and pancreatic carcinomas (Gibbs J. B., Cell, 65:1 (1991), Cartwright T., et al., Chimica. Oggi., 10:26 (1992)). These mutant ras proteins are deficient in the capability for feedback regulation that is present in native ras and this deficiency is associated with their oncogenic action since the ability to stimulate normal cell division cannot be controlled by the normal endogenous regulatory cofactors.
  • the recent discovery that the transforming activity of mutant ras is critically dependent on post-translational modifications (Gibbs J., et al., Microbiol. Rev., 53:171 (1989)) has unveiled an important aspect of ras function and identified novel prospects for cancer therapy.
  • Post-surgical vascular restenosis is such a condition.
  • the use of various surgical revascularization techniques such as saphenous vein bypass grafting, endarterectomy and transluminal coronary angioplasty is often accompanied by complications due to uncontrolled growth of neointimal tissue, known as restenosis.
  • the biochemical causes of restenosis are poorly understood and numerous growth factors and protooncogenes have been implicated (Naftilan A. J., et al., Hypertension, 13:706 (1989) and J. Clin. Invest., 83:1419; Gibbons G.
  • ras proteins are known to be involved in cell division processes makes them a candidate for intervention in many situations where cells are dividing uncontrollably.
  • blockade of ras dependent processes has the potential to reduce or eliminate the inappropriate tissue proliferation associated with restenosis, particularly in those instances where normal ras expression and/or function is exaggerated by growth stimulatory factors.
  • Ras functioning is dependent upon the modification of the proteins in order to associate with the inner face of plasma membranes.
  • ras proteins lack conventional transmembrane or hydrophobic sequences and are initially synthesized in a cytosol soluble form.
  • Ras protein membrane association is triggered by a series of post-translational processing steps that are signaled by a carboxyl terminal amino acid consensus sequence that is recognized by protein farnesyltransferase (PFT).
  • PFT protein farnesyltransferase
  • the sulfhydryl group of the cysteine residue is alkylated by farnesylpyrophosphate in a reaction that is catalyzed by protein farnesyltransferase. Following prenylation, the C-terminal three amino acids are cleaved by an endoprotease and the newly exposed alpha-carboxyl group of the prenylated cysteine is methylated by a methyl transferase.
  • the enzymatic processing of ras proteins that begins with farnesylation enables the protein to associate with the cell membrane. Mutational analysis of oncogenic ras proteins indicate that these post-translational modifications are essential for transforming activity.
  • PFTs protein farnesyltransferases
  • FPTs farnesyl proteintransferases
  • the enzyme was characterized as a heterodimer composed of one alpha-subunit (49 kDa) and one beta-subunit (46 kDa), both of which are required for catalytic activity.
  • High level expression of mammalian PFT in a baculovirus system and purification of the recombinant enzyme in active form has also been accomplished (Chen W. -J., et al., J. Biol. Chem., 268:9675 (1993)).
  • FTI-276 farnesyltransferase inhibitor
  • European Published Patent Application 0520823 discloses cysteine containing tetrapeptide inhibitors of PFT of the Formula Cys-Xaa 1 -dXaa 2 -Xaa 3 .
  • European Published Patent Application 0528486 discloses cysteine containing tetrapeptide amides inhibitors of PFT of the Formula Cys-Xaa 1 -Xaa 2 -Xaa 3 -NRR 1 .
  • Copending U.S. patent application Ser. No. 08/268,364 discloses a series of histidine and homohistidine derivatives as inhibitors of protein farnesyltransferase.
  • U.S. Pat. No. 4,870,183 discloses a series of amino acid derivatives of the Formula I: ##STR3## wherein Ar represents a phenyl group, a naphthyl group, or pyridyl group which may have a substituent. His represents an L-histidyl group, Y represents --O-- or --NH--, R represents a straight or branched chain alkyl group, a cycloalkyl group or a halogenated alkyl group, or pharmaceutically acceptable acid addition salts thereof, useful for treatment of hypertension, especially renin-associated hypertension. Additionally, U.S. Pat. No. 4,870,183 discloses intermediates of Formula IV: ##STR4## wherein the carbon atom marked with (S) is of S-- configuration and Ar is as defined above which are used to prepare compounds of Formula I.
  • U.S. Pat. No. 4,904,660 discloses a series of histidine derivatives useful as renin inhibitors in the treatment of hypertension. Additionally, U.S. Pat. No. 4,904,660 discloses intermediates of formula ##STR5## wherein R 1 is ##STR6## and R 2 is ##STR7## which are used to prepare the target renin inhibitors.
  • a series of substituted histidines are inhibitors of farnesyltransferase and thus useful as agents for the treatment of proliferative diseases such as, for example, cancer, restenosis, and psoriasis, and as antiviral agents.
  • R is hydrogen or alkyl
  • R 1 and R 2 may be the same or different and are selected from the group consisting of: ##STR9## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and R 7 may be 1 to 3 substituents selected from the group consisting of:
  • R 8 and R 9 may be the same or different and are selected from the group consisting of:
  • R 8 and R 9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is hydrogen or alkyl, and
  • n is zero or an integer of one to four, ##STR11## wherein R 9a is hydrogen or alkyl and R 8 , R 9 , and n are as defined above, and ##STR12## wherein R 11 is selected from the group consisting of: hydrogen,
  • aryl ##STR13## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
  • R 12 is hydrogen, alkyl, or ##STR14## wherein R 8 , R 9 , and n are as defined above, or R 12 is absent,
  • Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH 2 unit, and R 12 is absent, and R 7 is as defined above, ##STR15## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R 7 are as defined above, and R 12 may be present, ##STR16## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R 7 are as defined above, and R 12 may be present, ##STR17## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R 7 is as defined above with the proviso that R 1 and R 2 are not both a monocyclic ring, and ##STR18## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R 7 and Z are as defined above with the proviso that R 1 and R 2 are not both a monocyclic ring;
  • R 3 is hydrogen or alkyl
  • R 4 is selected from the group consisting of:
  • alkyl chain wherein the alkyl chain may be interrupted by a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above, ##STR19## wherein p is an integer of one to four, and R 13 is alkyl or benzyl, and ##STR20## wherein p and R 13 are as defined above; X is ##STR21## Y is ##STR22## R 5 is selected from the group consisting of: --OR 14 wherein R 14 is selected from the group consisting of:
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 15 and R 16 are as defined above, --S--R 14 wherein R 14 is as defined above with the proviso that R 14 is not hydrogen, ##STR25## wherein R 17 and R 18 may be the same or different and are selected from the group consisting of:
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 17 and R 18 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 6 is hydrogen
  • R is as defined above, or ##STR27## wherein R and R a may be the same or different and are as defined above for R;
  • R 18 may be ##STR28## wherein R is as defined above, or ##STR29## wherein R is as defined above; and when X is ##STR30## must be ##STR31## and excluding the compound wherein
  • R is hydrogen
  • R 1 and R 2 are each ##STR32##
  • R 3 is hydrogen, R 4 is hydrogen, ##STR33##
  • R 5 is OR 14 wherein R 14 is hydrogen, and R 6 is hydrogen;
  • the compounds of Formula I are antiproliferative agents. Thus, they are useful for the treatment of cancer, restenosis, and psoriasis, and as antiviral agents. Additionally, a compound of Formula I may be combined with other conventional anti-cancer agents such as, for example, cisplatin.
  • a still further embodiment of the present invention is a pharmaceutical composition for administering an effective amount of a compound of Formula I in unit dosage form in the treatment methods mentioned above.
  • the present invention is directed to methods for production of a compound of Formula I.
  • alkyl means a straight or branched hydrocarbon radical having from 1 to 12 carbon atoms and includes, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, undecyl, dodecyl, and the like.
  • alkenyl means a straight or branched unsaturated hydrocarbon radical having from 2 to 12 carbon atoms and includes, for example, ethenyl, 2-propenyl, 1-butenyl, 2-butenyl, 1-pentenyl, 2-pentenyl, 3-methyl-3-butenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 3-heptenyl, 1-octenyl, 1-nonenyl, 1-decenyl, 1-undecenyl, 1-dodecenyl, and the like.
  • alkynyl means a straight or branched triple bonded unsaturated hydrocarbon radical having from 2 to 12 carbon atoms and includes, for example, ethynyl, 2-propynyl, 3-butynyl, 4-pentynyl, 5-hexynyl, 6-heptynyl, 7-octynyl, 8-nonynyl, 9-decynyl, 10-undecynyl, 11-dodecynyl, and the like.
  • cycloalkyl means a saturated hydrocarbon ring which contains from 3 to 12 carbon atoms, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, adamantyl, and the like.
  • cycloalkylalkyl means a saturated hydrocarbon ring attached to an alkyl group wherein alkyl is as defined above.
  • the saturated hydrocarbon ring contains from 3 to 12 carbon atoms. Examples of such are cyclopropylmethyl, cyclopentylmethyl, cyclohexylmethyl, adamantylmethyl and the like.
  • alkoxy and thioalkoxy are O-alkyl or S-alkyl as defined above for alkyl.
  • aryl means an aromatic radical which is a phenyl group, a naphthyl group, a phenyl group substituted by 1 to 4 substituents selected from alkyl as defined above, alkoxy as defined above, thioalkoxy as defined above, hydroxy, halogen, trifluoromethyl, amino, alkylamino as defined above for alkyl, dialkylamino as defined for alkyl, N-acetylamino, cyano --SO 2 NH 2 , or nitro, or a naphthyl group substituted by 1 to 4 substituents as defined above for a phenyl group substituted by 1 to 4 substituents.
  • heteroaryl means a heteroaromatic radical which is 2- or 3-thienyl; 2- or 3-furanyl; 1-, 2- or 3-pyrrolyl; 1-, 2-, 4-, or 5-imidazolyl; 1-, 3-, 4-, or 5-pyrazolyl; 2-, 4-, or 5-thiazolyl; 3-, 4-, or 5-isothiazolyl; 2-, 4-, or 5-oxazolyl; 3-, 4-, or 5-isoxazolyl; 1-, 3-, or 5-1,2,4-triazolyl; 1-, 2-, 4-, or 5-1,2,3-triazolyl; 1- or 5-tetrazolyl; 4-, or 5-1,2,3-oxadiazolyl; 3-, or 5-1,2,4-oxadiazolyl; 2-1,3,4-oxadiazolyl; 2-1,3,4-thiadiazoyl; 2-1,3,5-triazinyl; 3-pyridinyl; 3-, 4-, or 5-pyridazinyl;
  • arylalkyl means an aromatic radical attached to an alkyl radical wherein aryl and alkyl are as defined above, for example, benzyl, naphthylmethyl, 4-sulfamoylphenylmethyl and the like.
  • heteroarylalkyl means a heteroaromatic radical attached to an alkyl radical wherein heteroaryl and alkyl are as defined above, for example, 2-imidazol-1-yl-ethyl, 3-imidazol-1-yl-propyl, 2-(1H-imidazol-4-yl)-ethyl, 2-butyl-1H-imidazol-4-ylmethyl, 2-thiophen-2-yl-ethyl, thiophen-3-ylmethyl, and the like.
  • Halogen is fluorine, chlorine, bromine, or iodine.
  • haloalkyl means a halogen atom attached to an alkyl radical wherein halogen and alkyl are as defined above.
  • hydroxyalkyl means a hydroxy group attached to an alkyl radical wherein alkyl is as defined above.
  • mercaptoalkyl means a mercapto group attached to an alkyl radical wherein alkyl is as defined above.
  • cyanoalkyl means a cyano group attached to an alkyl radical wherein alkyl is as defined above.
  • nitroalkyl means a nitro group attached to an alkyl radical wherein alkyl is as defined above.
  • alkoxyalkyl means an alkoxy group attached to an alkyl radical wherein alkoxy and alkyl are as defined above.
  • thioalkoxyalkyl means a thioalkoxy group attached to an alkyl radical wherein thioalkoxy and alkyl are as defined above.
  • acetamidoalkyl means a ##STR34## group attached to an alkyl radical wherein alkyl is as defined above.
  • benzyloxyalkyl means a benzyloxy group attached to an alkyl radical wherein alkyl is as defined above.
  • the ring designated: ##STR35## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR36## and the like mono- to trisubstituted by R 7 which may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms and unsaturated carbon atoms wherein the --CH 2 -- group is attached, and R 7 is selected from hydrogen, alkyl, alkenyl, alkoxy, thioalkoxy, hydroxy, mercapto, halogen, nitro, ##STR37## wherein R 8 and R 9 may be the same or different and are selected from the group consisting of:
  • R 8 and R 9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is hydrogen or alkyl, n is zero or an integer of one to four, ##STR38## wherein R 9a is hydrogen or alkyl and R 8 , R 9 , and n are as defined above, and ##STR39## wherein R 11 is selected from the group consisting of hydrogen, alkyl, and aryl wherein alkyl, alkenyl, alkoxy, thioalkoxy, halogen, aryl, and R 7 are as defined above.
  • the ring designated: ##STR40## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, wherein Z is selected from the group consisting of: NR 12 wherein R 12 is hydrogen or alkyl or ##STR41## wherein R 8 , R 9 , and n are as defined above, or R 12 is absent, O, S, SO, and SO 2 and Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring system is aromatic, Z is not at the point of attachment of the CH 2 -- unit, and R 12 is absent, for example: ##STR42## and the like mono- to trisubstituted by R 7 as defined above wherein R 7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms and unsaturated carbon atoms wherein the --CH 2 -- group is attached.
  • the ring designated: ##STR43## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR44## and the like mono- and trisubstituted by R 7 as defined above wherein R 7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms, and unsaturated carbon atoms wherein the --CH 2 -- group is attached, and Z is as defined above and R 12 may be present.
  • the ring designated: ##STR45## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR46## and the like mono- and trisubstituted by R 7 as defined above wherein R 7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms, and unsaturated carbon atoms wherein the --CH 2 -- is attached, and Z is as defined above and R 12 may be present.
  • the compounds of Formula I are capable of further forming both pharmaceutically acceptable acid addition and/or base salts. All of these forms are within the scope of the present invention.
  • Pharmaceutically acceptable acid addition salts of the compounds of Formula I include salts derived from nontoxic inorganic acids such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydriodic, hydrofluoric, phosphorous, and the like, as well as the salts derived from nontoxic organic acids, such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids, aliphatic and aromatic sulfonic acids, etc.
  • nontoxic inorganic acids such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydriodic, hydrofluoric, phosphorous, and the like
  • nontoxic organic acids such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids
  • Such salts thus include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, nitrate, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, trifluoroacetate, propionate, caprylate, isobutyrate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, mandelate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, phthalate, benzenesulfonate, toluenesulfonate, phenylacetate, citrate, lactate, maleate, tartrate, methanesulfonate, and the like.
  • salts of amino acids such as arginate and the like and gluconate, galacturonate (see, for example, Berge S. M., et al., "Pharmaceutical Salts,” J. of Pharma. Sci., 66:1 (1977)).
  • the acid addition salts of said basic compounds are prepared by contacting the free base form with a sufficient amount of the desired acid to produce the salt in the conventional manner.
  • the free base form may be regenerated by contacting the salt form with a base and isolating the free base in the conventional manner.
  • the free base forms differ from their respective salt forms somewhat in certain physical properties such as solubility in polar solvents, but otherwise the salts are equivalent to their respective free base for purposes of the present invention.
  • Pharmaceutically acceptable base addition salts are formed with metals or amines, such as alkali and alkaline earth metals or organic amines.
  • metals used as cations are sodium, potassium, magnesium, calcium, and the like.
  • suitable amines are N,N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, dicyclohexylamine, ethylenediamine, N-methylglucamine, and procaine (see, for example, Berge S. M., et al., "Pharmaceutical Salts," J. of Pharma. Sci., 66:1 (1977)).
  • the base addition salts of said acidic compounds are prepared by contacting the free acid form with a sufficient amount of the desired base to produce the salt in the conventional manner.
  • the free acid form may be regenerated by contacting the salt form with an acid and isolating the free acid in the conventional manner.
  • the free acid forms differ from their respective salt forms somewhat in certain physical properties such as solubility in polar solvents, but otherwise the salts are equivalent to their respective free acid for purposes of the present invention.
  • Certain of the compounds of the present invention can exist in unsolvated forms as well as solvated forms, including hydrated forms.
  • the solvated forms, including hydrated forms are equivalent to unsolvated forms and are intended to be encompassed within the scope of the present invention.
  • Certain of the compounds of the present invention possess one or more chiral centers and each center may exist in the R(D) or S(L) configuration.
  • the present invention includes all enantiomeric and epimeric forms as well as the appropriate mixtures thereof.
  • a preferred compound of Formula I is one wherein
  • R is hydrogen
  • R 7 is selected from the group consisting of:
  • R 8 and R 9 may be the same or different and are selected from the group consisting of:
  • R 3 is hydrogen or methyl
  • R 4 is selected from the group consisting of:
  • X is ##STR52## or Y is ##STR53## or R 5 is selected from the group consisting of:
  • R 14 is selected from the group consisting of:
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: O, and NR 10 wherein R 10 is hydrogen or methyl, and
  • R 14 is as defined above with the proviso that R 14 is not hydrogen, and ##STR55## wherein R 19 is hydrogen,
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above, and
  • R 20 is hydrogen or methyl
  • R 6 is hydrogen
  • a more preferred compound of Formula I is one wherein R 1 and R 2 may be the same or different and are selected from the group consisting of: ##STR57## R 3 is hydrogen or methyl; R 4 is hydrogen, methyl, or --CH 2 OCH 3 ;
  • X is --CH 2 -- or ##STR58## and R 5 is selected from the group consisting of:
  • R 14 is selected from the group consisting of:
  • R 14 is as defined above with the proviso that R 14 is not hydrogen, and ##STR60## wherein R 17 is selected from the group consisting of: ##STR61## R 18 is hydrogen or methyl, and --NH--OR 10 wherein R 10 is hydrogen or methyl.
  • the compounds of Formula I are valuable inhibitors of the enzyme farnesyltransferase.
  • the protein:farnesyltransferase (PFT) or farnesyl protein transferase (FPT) inhibitory activity of compounds of Formula I was assayed in HEPES buffer (pH 7.4) containing 5 mM potassium phosphate and 20 ⁇ M ZnCl 2 .
  • the solution also contained 7 mM DTT, 1.2 mM MgCl 2 .
  • Assays were performed in 96 well plates (Wallec) and employed solutions composed of varying concentrations of a compound of Formula I in 100% DMSO.
  • radiolabeled farnesyl pyrophosphate [1- 3 H], specific activity 15-30 Ci/mmol, final concentration 0.12 ⁇ M
  • biotinyl)-Ahe-Tyr-Lys-Cys-Val-Ile-Met [3aS[3a alpha, 4 beta, 6a alpha]-hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-5-pentanoic acid]-[7-aminoheptanoic acid]-Thr-Lys-Cys-Val-Ile-Met
  • the enzyme reaction was started by addition of 40-fold purified rat brain farnesyl protein transferase.
  • the farnesylation inhibitor is added at varying concentrations. Following an 18-hour incubation period, cells are lysed in phosphate-buffered saline containing 1% Triton X-100, 0.5% sodium deoxycholate, and 0.1% SDS, pH 7.4 in the presence of several protease inhibitors (PMSF, antipain, leupeptin, pepstatin A, and aprotinin all at 1 ⁇ g/mL). Ras protein is immunoprecipitated from the supernatants by the addition of 3 ⁇ g v-H-ras Ab-2 (Y13-259 antibody from Oncogene Science).
  • R is hydrogen or alkyl
  • R 1 and R 2 may be the same or different and are selected from the group consisting of: ##STR63## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and R 7 may be 1 to 3 substituents selected from the group consisting of:
  • R 8 and R 9 may be the same or different and are selected from the group consisting of:
  • R 8 and R 9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of:
  • R 10 is hydrogen or alkyl
  • n is zero or an integer of one to four, ##STR65## wherein R 9a is hydrogen or alkyl and R 8 , R 9 , and n are as defined above, and ##STR66## wherein R 11 is selected from the group consisting of: hydrogen,
  • aryl ##STR67## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
  • R 12 is hydrogen, alkyl, or ##STR68## wherein R 8 , R 9 , and n are as defined above, or R 12 is absent,
  • Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH 2 unit and R 12 is absent, and R 7 is as defined above, ##STR69## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R 7 are as defined above and R 12 may be present, ##STR70## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R 7 are as defined above, and R 12 may be present, ##STR71## wherein the monocyclic may be aromatic, or partially or completely saturated, and R 7 is as defined above with the proviso that R 1 and R 2 are not both a monocyclic ring, and ##STR72## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R 7 and Z are as defined above with the proviso that R 1 and R 2 are not both a monocyclic ring;
  • R 3 is hydrogen or alkyl
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 15 and R 16 are as defined above, --S--R 14a wherein R 14a is as defined above; ##STR77## wherein R 17 and R 18 may be the same or different and are selected from the group consisting of:
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 17 and R 18 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • R 6 is hydrogen
  • R is as defined above, or ##STR79## wherein R and R a may be the same or different and are as defined above for R;
  • Trt is (C 6 H 5 ) 3 --C-- and R, R 1 , R 2 , R 3 , X a , Y, R 5a and R 6 are as defined above with an acid such as, for example, 80% to 85% acetic acid and the like at about 90° C. for about 0.5 hours to afford a compound of Formula Ia.
  • the reaction is carried out with 80% to 85% acetic aced at about 90° C. for about 0.5 hours.
  • a compound of Formula Ib ##STR81## wherein R 5b is OH and R, R 1 , R 2 , R 3 , X a , and R 6 as defined above may be prepared by reacting a compound of Formula Ic ##STR82## wherein R 5c is OCH 3 or SCH 3 and R, R 1 , R 2 , R 3 , X a , R 6 and Trt are as defined above with a base such as, for example, 1N sodium hydroxide and the like in a solvent such as, for example, methanol, dioxane, and the like at about room temperature for about 2 hours to afford a compound of Formula Ic-1.
  • a base such as, for example, 1N sodium hydroxide and the like
  • a solvent such as, for example, methanol, dioxane, and the like
  • a compound of Formula Id ##STR84## wherein X b is --CH 2 -- and R, R 1 , R 2 , R 3 , R 5b , and R 6 are as defined above may be prepared by reacting a compound of Formula Ia with a metal hydride such as, for example, lithium borohydride and the like in a solvent such as, for example, tetrahydrofuran and the like at about 0° C. to about room temperature to afford a compound of Formula Id.
  • a metal hydride such as, for example, lithium borohydride and the like
  • a solvent such as, for example, tetrahydrofuran and the like
  • the reaction is carried out with lithium borohydride in tetrahydrofuran at about 25° C.
  • R 5c-1 is OR 14a wherein R 14a is selected from the group consisting of:
  • R 15 and R 16 may be the same or different and are selected from the group consisting of:
  • alkyl or R 15 and R 16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is as defined above,
  • alkyl-O 2 C-alkyl and ##STR87## wherein R 15 and R 16 are as defined above; and R, R 1 , R 2 , R 3 , X b , and R 6 are as defined above may be prepared by reacting a compound of Formula III ##STR88## wherein R, R 1 , and R 2 are as defined above with a compound of Formula IV ##STR89## wherein X b , R 5c-1 , and R 6 are as defined above in the presence of a coupling reagent such as, for example, N,N-dicyclohexylcarbodiimide (DCC) and 1-hydroxybenzotriazole (HOBt) and the like in a solvent such as, for example, tetrahydrofuran (THF) at about room temperature for about 12 hours to afford a compound of Formula Ie.
  • a coupling reagent such as, for example, N,N-dicyclohexylcarbodiimide (DCC) and 1-hydroxybenz
  • a compound of Formula I f ##STR90## wherein R 17 is ##STR91## R, R 1 , R 2 , R 3 , X b , and R 6 are as defined above may be prepared from a compound of Formula V ##STR92## wherein R, R 1 , R 2 , R 3 , X b , R 6 , R 17 , and Trt are as defined above using methodology used to prepare a compound of Formula Ia from a compound of Formula II to afford a compound of Formula I f .
  • R, R 1 , R 2 , R 3 , and R 6 are as defined above may be prepared by reacting a compound of Formula III with a compound of Formula VI ##STR94## wherein R 5d and R 6 are as defined above using methodology used to prepare a compound of Formula Ie from a compound of Formula III and a compound of Formula IV to afford a compound of Formula I g .
  • R 4a is selected from the group consisting of: alkyl
  • alkyl chain wherein the alkyl chain may be interrupted by a heteroatom selected from the group consisting of: S, O, and N--R 10 wherein R 10 is hydrogen or alkyl, ##STR96## wherein p is an integer of one to four, and R 13 is alkyl or benzyl, and ##STR97## wherein p and R 13 are as defined above, and R, R 1 , R 2 , R 3 , X a , R 5a , and R 6 are as defined above may be prepared from a compound of Formula VII ##STR98## wherein R, R 1 , R 2 , R 3 , R 4a , R 5a , R 6 , X a , and Trt are as defined above in the presence of 80% acetic acid to afford a compound of Formula I h .
  • a compound of Formula I i ##STR99## wherein R, R 1 , R 2 , R 3 , R 4a , R 5a , R 6 , and X a are as defined above may be prepared from a compound of Formula I h in the presence of Lawesson's Reagent (2,4-bis(4-methoxyphenyl)-1,3-dithia-2,4-diphosphetane-2,4-disulfide) and pyridine to afford a compound of Formula I i
  • a compound of Formula I j ##STR100## wherein R, R 1 , R 2 , R 4a , R 5a , R 6 , and X a are as defined above by reacting a compound of Formula VIII ##STR101## wherein R, R 1 , and R 2 are as defined above with a compound of Formula IX ##STR102## wherein R 4a , R 5a , R 6 , and X a are as defined above in the presence of a metal hydride such as, for example, sodium cyanoborohydride and the like in the presence of molecular sieves to afford a compound of Formula I j .
  • a metal hydride such as, for example, sodium cyanoborohydride and the like in the presence of molecular sieves to afford a compound of Formula I j .
  • a compound of Formula I k ##STR103## wherein Y is --CH 2 --, ##STR104## R, R 1 , R 2 , R 4a , R 6 , and R 17 are as defined above may be prepared from a compound of Formula X. ##STR105## wherein R, R 1 , R 2 , R 4a , R 6 , and Y are as defined above and a compound of Formula XI
  • R 17 is as defined above using the methodology used to prepare a compound of Formula I j from a compound of Formula VIII and a compound of Formula IX to afford a compound of Formula I k .
  • a compound of Formula I l ##STR106## wherein R, R 1 , R 2 , R 4a , R 6 , and R 17 are as defined above may be prepared from a compound of Formula III and a compound of Formula XII ##STR107## wherein R 4a , R 6 , and R 17 are as defined above using methodology used to prepare a compound of Formula I e from a compound of Formula III and a compound of Formula IV to afford a compound of Formula I l .
  • a compound of Formula II may be prepared from a compound of Formula XIII ##STR108## wherein R, R 1 , R 2 , R 3 , R 6 , Y, and Trt are as defined above and a compound of Formula XIV
  • R 5a is as defined above using standard methodology such as the methodology used to prepare a compound of Formula I e from a compound of Formula III and a compound of Formula IV to afford a compound of Formula II.
  • a compound of Formula XIII may be prepared from a compound of Formula XV ##STR109## wherein R, R 1 , R 2 , R 3 , R 6 , Y, and Trt are as defined above in the presence of a dilute base such as, for example, dilute aqueous sodium hydroxide and the like at room temperature to afford a compound of Formula XIII.
  • a dilute base such as, for example, dilute aqueous sodium hydroxide and the like at room temperature to afford a compound of Formula XIII.
  • a compound of Formula XV a ##STR110## wherein R, R 1 , R 2 , R 3 , R 6 , and Trt are as defined above may be prepared by reacting a compound of Formula III and a compound of Formula XVI ##STR111## wherein R 3 , R 6 , and Trt are as defined above using methodology used to prepare a compound of Formula I e from a compound of Formula III and a compound of Formula IV to afford a compound of Formula XV a .
  • a compound of Formula XV b ##STR112## wherein R, R 1 , R 2 , R 3 , R 6 , and Trt are as defined above may be prepared from a compound of Formula XV a using the methodology used to prepare compound of Formula I i from a compound of Formula I h to afford a compound of Formula XV b .
  • a compound of Formula XV c ##STR113## wherein R, R 1 , R 2 , R 3 , R 6 , and Trt are as defined above may be prepared from a compound of Formula VIII and a compound of Formula XVI using the methodology used to prepare a compound of Formula I j from a compound of Formula VIII and a compound of Formula IX to afford a compound of Formula XV c .
  • a compound of Formula VIII may be prepared from a compound of Formula XVII ##STR114## wherein R, R 1 , and R 2 are as defined above by treatment with a metal hydride such as, for example, lithium aluminum hydride and the like at about 0° C. to afford a compound of Formula VIII.
  • a metal hydride such as, for example, lithium aluminum hydride and the like at about 0° C.
  • a compound of Formula XVII may be prepared from a compound of Formula III and N,0-dimethyl-hydroxylamine in the presence of methyl chloroformate to afford a compound of Formula XVII.
  • a compound of Formula III may be prepared from a compound of Formula XVIII ##STR115## wherein R, R 1 , and R 2 are as defined above using methodology used to prepare a compound of Formula XIII from a compound of Formula XV to afford a compound of Formula III.
  • a compound of XVIII a wherein R a is alkyl and R 1 and R 2 are as defined above may be prepared by reacting a compound of Formula XIX ##STR117## wherein R 1 and R 2 are as defined above with a compound of Formula XX
  • R a is as defined above in the presence of a base such as, for example, sodium hydride and the like to afford a compound of Formula XVIII a .
  • a compound of Formula XIX may be prepared from a compound of Formula XXI ##STR118## wherein R 1 and R 2 are as defined above in the presence of about one equivalent of a base such as, for example, sodium hydroxide and the like and a solvent such as, for example, dioxane and the like, and after acidification with an acid such as, for example, hydrochloric acid and the like heating to effect decarboxylation to afford a compound of Formula XIX.
  • a base such as, for example, sodium hydroxide and the like and a solvent such as, for example, dioxane and the like
  • a compound of Formula XXI may be prepared from a compound of Formula XXII ##STR119## wherein R 1 is as defined above by reaction with a compound of Formula XXIII
  • R 2 is as defined above using methodology used to prepare a compound of Formula XVIII a from a compound of Formula XIX and a compound of Formula XX to afford a compound of Formula XXI.
  • a compound of Formula XXII may be prepared from a dialkylmalonate in the presence of a compound of Formula XXIV
  • R 1 is as defined above using methodology used to prepare a compound of Formula XVIII a from a compound of Formula XIX and a compound of Formula XX to afford a compound of Formula XXII.
  • a compound of Formula XXIV may be prepared from a compound of Formula XXV
  • R 1 is as defined above by treatment with PBr 3 to afford a compound of Formula XXIV.
  • a compound of Formula XXV may be prepared from a compound of Formula XXVI
  • R 1a is R 1 in which the CH 2 group is absent by treatment with a metal hydride such as, for example, lithium aluminum hydride and the like at room temperature to afford a compound of Formula XXV.
  • a metal hydride such as, for example, lithium aluminum hydride and the like at room temperature to afford a compound of Formula XXV.
  • a compound of Formula IV may be prepared from a compound of Formula XXVII ##STR120## wherein Boc is tertiary butoxycarbonyl, and R 5c-1 , R 6 , and X b are as defined above by treatment with an acid such as, for example, trifluoroacetic acid and the like in the presence of a solvent such as, for example, dichloromethane and the like to afford a compound of Formula IV.
  • a compound of Formula XXVII may be prepared from a compound of Formula XXVIII ##STR121## wherein Boc and X b are as defined above by reaction with a compound of Formula XXIX
  • R 5c-1 is as defined above in the presence of a base such as, for example, sodium hydride and the like to afford a compound of Formula XXVII.
  • a compound of Formula XXVIII may be prepared from a compound of Formula XXX ##STR122## wherein Boc is as defined above by treatment with a metal hydride reagent such as, for example, lithium aluminum hydride and the like in a solvent such as, for example, tetrahydrofuran and the like to afford a compound of Formula XXVIII.
  • a metal hydride reagent such as, for example, lithium aluminum hydride and the like in a solvent such as, for example, tetrahydrofuran and the like to afford a compound of Formula XXVIII.
  • a compound of Formula V a ##STR123## wherein R 17a is ##STR124## and R, R 1 , R 2 , R 3 , R 6 , X b , and Trt are as defined above may be prepared from a compound of Formula XXXI ##STR125## wherein R, R 1 , R 2 , R 3 , R 6 , X b , and Trt are as defined above by reaction with a compound of Formula XXXII
  • R 17a is as defined above in the presence of a base such as, for example, triethylamine and the like to afford a compound of Formula V a .
  • a compound of Formula XXXI may be prepared from a compound of Formula XXXIII ##STR126## wherein R, R 1 , R 2 , R 3 , R 6 , and Trt are as defined above by treatment with ammonia in the presence of a metal hydride such as, for example, sodium cyanoborohydride and the like and a solvent such as, for example, 2-propanol and the like to afford a compound of Formula XXXI.
  • a metal hydride such as, for example, sodium cyanoborohydride and the like
  • solvent such as, for example, 2-propanol and the like
  • a compound of Formula V b ##STR127## wherein R 17b is ##STR128## and R, R 1 , R 2 , R 3 , R 6 , X b , and Trt are as defined above may be prepared from a compound of Formula XXXI by treatment with a compound of Formula XXXIV
  • R is as defined above in the presence of a base such as, for example, triethylamine and the like to afford a compound of Formula V b .
  • a compound of Formula VI may be prepared from a compound of Formula XXXV ##STR129## wherein R 5d , R 6 , and Trt are as defined above by treatment with 80% acetic acid at about 90° C. for about 0.5 hour to afford a compound of Formula VI.
  • a compound of Formula XXXV may be prepared from a compound of Formula XXXVI ##STR130## wherein R 6 and Trt are as defined above by treatment with about 2 mol of a compound of Formula XXXVII
  • R 5d is as defined above in the presence of a solvent such as, for example, tetrahydrofuran and the like to afford a compound of Formula XXXV.
  • a compound of Formula VII may be prepared from a compound of Formula XXXVIII ##STR131## wherein R, R 1 , R 2 , R 3 , R 5a , R 6 , X a , and Trt are as defined above by treatment with a compound of Formula XXXIX
  • R 4a is as defined above in the presence of a solvent such as, for example, dichloromethane and the like to afford a compound of Formula VII.
  • a compound of Formula IX may be prepared from a compound of Formula XL ##STR132## wherein R 6 , Boc, and Trt are as defined above by treatment with a compound of Formula XXXIX followed by removal of the Trt group with 80% acetic acid at about 90° C. for about 0.5 hour and subsequent removal of the Boc group with an acid such as, for example, hydrogen chloride gas and the like in a solvent such as, for example, dichloromethane and the like to afford a compound of Formula IX.
  • a compound of Formula X may be prepared from a compound of Formula XLI ##STR133## wherein R, R 1 , R 2 , R 4a , R 6 , and Y are as defined above by treatment with methyl chloroformate, N,O-dimethylhydroxylamine, and piperidine followed by treatment with lithium aluminum hydride in tetrahydrofuran to afford a compound of Formula X.
  • a compound of Formula XLI may be prepared from a compound of Formula XLII ##STR134## wherein R, R 1 , R 2 , R 4a , R 6 , and Y are as defined above by treatment with a dilute base such as, for example, dilute sodium hydroxide and the like to afford a compound of Formula XLI.
  • a dilute base such as, for example, dilute sodium hydroxide and the like
  • a compound of Formula XII may be prepared from a compound of Formula XLIII ##STR135## wherein Boc, R 4a , R 6 , and R 17 are as defined above by treatment with an acid such as, for example, hydrogen chloride gas and the like in a solvent such as, for example, dichloromethane and the line to afford a compound of Formula XII.
  • a compound of Formula XLIII may be prepared from a compound of Formula XLIV ##STR136## wherein Boc, R 4a , R 6 , and R 17 are as defined above using the methodology used to prepare a compound of Formula I i from a compound of Formula I h to afford a compound of Formula XLIII.
  • a compound of Formula XLIV may be prepared by reacting a compound of Formula XLV ##STR137## wherein Boc, R 4a , and R 6 are as defined above with a compound of Formula XI in the presence of methyl chloroformate and a base such as, for example, triethylamine and the like to afford a compound of Formula XLIV.
  • a compound of Formula III wherein R 1 and R 2 are bicyclic rings, which are partially or completely saturated, may be prepared from the corresponding aromatic bicyclic compound using conventional reducing conditions known in the art.
  • the compounds of the present invention can be prepared and administered in a wide variety of oral and parenteral dosage forms.
  • the compounds of the present invention can be administered by injection, that is, intravenously, intramuscularly, intracutaneously, subcutaneously, intraduodenally, or intraperitoneally.
  • the compounds of the present invention can be administered by inhalation, for example, intranasally.
  • the compounds of the present invention can be administered transdermally. It will be obvious to those skilled in the art that the following dosage forms may comprise as the active component, either a compound of Formula I or a corresponding pharmaceutically acceptable salt of a compound of Formula I.
  • pharmaceutically acceptable carriers can be either solid or liquid.
  • Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories, and dispersible granules.
  • a solid carrier can be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material.
  • the carrier is a finely divided solid which is in a mixture with the finely divided active component.
  • the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.
  • the powders and tablets preferably contain from five or ten to about seventy percent of the active compound.
  • Suitable carriers are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like.
  • the term "preparation" is intended to include the formulation of the active compound with encapsulating material as a carrier providing a capsule in which the active component, with or without other carriers, is surrounded by a carrier, which is thus in association with it.
  • cachets and lozenges are included. Tablets, powders, capsules, pills, cachets, and lozenges can be used as solid dosage forms suitable for oral administration.
  • a low melting wax such as a mixture of fatty acid glycerides or cocoa butter
  • the active component is dispersed homogeneously therein, as by stirring.
  • the molten homogenous mixture is then poured into convenient sized molds, allowed to cool, and thereby to solidify.
  • Liquid form preparations include solutions, suspensions, and emulsions, for example, water or water propylene glycol solutions.
  • liquid preparations can be formulated in solution in aqueous polyethylene glycol solution.
  • Aqueous solutions suitable for oral use can be prepared by dissolving the active component in water and adding suitable colorants, flavors, stabilizing, and thickening agents as desired.
  • Aqueous suspensions suitable for oral use can be made by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well-known suspending agents.
  • viscous material such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well-known suspending agents.
  • solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for oral administration.
  • liquid forms include solutions, suspensions, and emulsions.
  • These preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
  • the pharmaceutical preparation is preferably in unit dosage form.
  • the preparation is subdivided into unit doses containing appropriate quantities of the active component.
  • the unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules.
  • the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form.
  • the quantity of active component in a unit dose preparation may be varied or adjusted from 1 mg to 1000 mg, preferably 10 mg to 100 mg according to the particular application and the potency of the active component.
  • the composition can, if desired, also contain other compatible therapeutic agents.
  • the compounds utilized in the pharmaceutical method of this invention are administered at the initial dosage of about 1 mg to about 50 mg per kilogram daily.
  • a daily dose range of about 5 mg to about 25 mg per kilogram is preferred.
  • the dosages may be varied depending upon the requirements of the patient, the severity of the condition being treated, and the compound being employed. Determination of the proper dosage for a particular situation is within the skill of the art. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstance is reached. For convenience, the total daily dosage may be divided and administered in portions during the day if desired.
  • Step (b) Preparation of: (S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid methyl ester
  • Step (d) Preparation of: (S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, propyl ester
  • Step (b) Preparation of: (S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
  • the organic phase was diluted with EtOAc and washed with a saturated solution of NaHCO 3 , H 2 O, and a saturated solution of NaCl. Drying over MgSO 4 and removal of the solvent under reduced pressure left 7.27 g of the crude product as a cream foam. This was the trityl compound complexed with BH 2 CN.

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Abstract

PCT No. PCT/US97/00265 Sec. 371 Date Jul. 2, 1998 Sec. 102(e) Date Jul. 2, 1998 PCT Filed Jan. 2, 1997 PCT Pub. No. WO97/26246 PCT Pub. Date Jul. 24, 1997Substituted histidine compounds of formula (I) are described as well as methods for the preparation and pharamaceutical compositions of same, which are useful as inhibitors of protein famesyltransferase and for the treatment of proliferative diseases including cancer, restenosis, and psoriasis, and as antiviral agents.

Description

This application is a 371 of PCT/US97/00265 filed Jan. 2, 1997, and this application claims the benefit of U.S. Provisional Application No. 60/009,956 filed Jan. 16, 1996.
BACKGROUND OF THE INVENTION
The present invention relates to novel substituted histidine compounds useful as pharmaceutical agents, to methods for their production, to pharmaceutical compositions which include these compounds and a pharmaceutically acceptable carrier, and to pharmaceutical methods of treatment. The novel compounds of the present invention inhibit farnesyl transferase enzyme which activates ras proteins which in turn activate cellular division. More particularly, the novel compounds of the present invention are useful in the treatment of proliferative diseases such as, for example, cancer, restenosis, and psoriasis, and as antiviral agents.
Ras protein (or p21) has been examined extensively because mutant forms are found in 20% of most types of human cancer and greater than 50% of colon and pancreatic carcinomas (Gibbs J. B., Cell, 65:1 (1991), Cartwright T., et al., Chimica. Oggi., 10:26 (1992)). These mutant ras proteins are deficient in the capability for feedback regulation that is present in native ras and this deficiency is associated with their oncogenic action since the ability to stimulate normal cell division cannot be controlled by the normal endogenous regulatory cofactors. The recent discovery that the transforming activity of mutant ras is critically dependent on post-translational modifications (Gibbs J., et al., Microbiol. Rev., 53:171 (1989)) has unveiled an important aspect of ras function and identified novel prospects for cancer therapy.
In addition to cancer, there are other conditions of uncontrolled cellular proliferation that may be related to excessive expression and/or function of native ras proteins. Post-surgical vascular restenosis is such a condition. The use of various surgical revascularization techniques such as saphenous vein bypass grafting, endarterectomy and transluminal coronary angioplasty is often accompanied by complications due to uncontrolled growth of neointimal tissue, known as restenosis. The biochemical causes of restenosis are poorly understood and numerous growth factors and protooncogenes have been implicated (Naftilan A. J., et al., Hypertension, 13:706 (1989) and J. Clin. Invest., 83:1419; Gibbons G. H., et al., Hypertension, 14:358 (1989); Satoh T., et al., Mollec. Cell. Biol., 13:3706 (1993)). The fact that ras proteins are known to be involved in cell division processes makes them a candidate for intervention in many situations where cells are dividing uncontrollably. In direct analogy to the inhibition of mutant ras related cancer, blockade of ras dependent processes has the potential to reduce or eliminate the inappropriate tissue proliferation associated with restenosis, particularly in those instances where normal ras expression and/or function is exaggerated by growth stimulatory factors.
Ras functioning is dependent upon the modification of the proteins in order to associate with the inner face of plasma membranes. Unlike other membrane-associated proteins, ras proteins lack conventional transmembrane or hydrophobic sequences and are initially synthesized in a cytosol soluble form. Ras protein membrane association is triggered by a series of post-translational processing steps that are signaled by a carboxyl terminal amino acid consensus sequence that is recognized by protein farnesyltransferase (PFT). This consensus sequence consists of a cysteine residue located four amino acids from the carboxyl terminus, followed by two lipophilic amino acids and the C-terminal residue. The sulfhydryl group of the cysteine residue is alkylated by farnesylpyrophosphate in a reaction that is catalyzed by protein farnesyltransferase. Following prenylation, the C-terminal three amino acids are cleaved by an endoprotease and the newly exposed alpha-carboxyl group of the prenylated cysteine is methylated by a methyl transferase. The enzymatic processing of ras proteins that begins with farnesylation enables the protein to associate with the cell membrane. Mutational analysis of oncogenic ras proteins indicate that these post-translational modifications are essential for transforming activity. Replacement of the consensus sequence cysteine residue with other amino acids gives a ras protein that is no longer farnesylated, fails to migrate to the cell membrane and lacks the ability to stimulate cell proliferation (Hancock J. F., et al., Cell, 57:1167 (1989), Schafer W. R., et al., Science, 245:379 (1989), Casey P. J., Proc. Natl. Acad. Sci. USA, 86:8323 (1989)).
Recently, protein farnesyltransferases (PFTs, also referred to as farnesyl proteintransferases (FPTs) have been identified and a specific PFT from rat brain was purified to homogeneity (Reiss Y., et al., Bioch. Soc. Trans., 20:487-88 (1992)). The enzyme was characterized as a heterodimer composed of one alpha-subunit (49 kDa) and one beta-subunit (46 kDa), both of which are required for catalytic activity. High level expression of mammalian PFT in a baculovirus system and purification of the recombinant enzyme in active form has also been accomplished (Chen W. -J., et al., J. Biol. Chem., 268:9675 (1993)).
In light of the foregoing, the discovery that the function of oncogenic ras proteins is critically dependent on their post-translational processing provides a means of cancer chemotherapy through inhibition of the processing enzymes. The identification and isolation of a protein farnesyltransferase that catalyzes the addition of a farnesyl group to ras proteins provides a promising target for such intervention. Recently, it has been determined that prototypical inhibitors of PPT can inhibit ras processing and reverse cancerous morphology in tumor cell models (Kohl N. E., et al., Science, 260:1934 (1993), James G. L., et al., Science, 260:1937 (1993), Garcia A. M., et al., J. Biol. Chem., 268:18415 (1993)). Furthermore, Blaskovich M., et al., "Proceedings Eighty-Sixth Annual Meeting American Association For Cancer Research," Mar. 18-22, 1995, Toronto, Ontario, Canada, Vol. 86, March 1995, Abstract 2578, disclosed a series of tetrapeptide inhibitors of farnesyltransferase which inhibited growth of tumor cells in nude mice.
Nagasu T., et al., "Proceedings Eighty-Sixth Annual Meeting American Association For Cancer Research," Mar. 18-22, 1995, Toronto, Ontario, Canada, Vol. 86, March 1995, Abstract 2615, disclosed a peptidomimetic inhibitor, B956, of farnesyltransferase which inhibits growth of human tumor xenografts in nude mice. Kohl, N. E., et al., Proc. Natl. Acad. Sci. USA, 91:9141 (1994) have demonstrated that the protein farnesyltransferase inhibitor (L-739,749) blocks the growth of a ras-dependent tumor in nude mice. Sebti, S. M., et al., Cancer Research, 55:4243 (1995) have demonstrated that a farnesyltransferase inhibitor (FTI-276) blocks the growth in nude mice of a human lung carcinoma with a k-ras mutation. Inhibition of tumor growth is correlated with inhibition of ras processing.
Thus, it is possible to prevent or delay the onset of cellular proliferation in cancers that exhibit mutant ras proteins by blocking PFT. By analogous logic, inhibition of PFT would provide a potential means for controlling cellular proliferation associated with restenosis, especially in those cases wherein the expression and/or function of native ras is overstimulated. Indolfi, C., et al., Nature Medicine, 1:541 (1995) have demonstrated that inhibition of cellular ras prevents smooth muscle cell proliferation after vascular injury in the rat.
PCT Published Patent Application WO91/16340 discloses cysteine containing tetrapeptide inhibitors of PFT of the Formula CAAX.
European Published Patent Application 0461869 discloses cysteine containing tetrapeptide inhibitors of PFT of the Formula Cys-Aaa1 -Aaa2 -Xaa.
European Published Patent Application 0520823 discloses cysteine containing tetrapeptide inhibitors of PFT of the Formula Cys-Xaa1 -dXaa2 -Xaa3.
European Published Patent Application 0523873 discloses cysteine containing tetrapeptide inhibitors of PFT of the Formula Cys-Xaa1 -Xaa2 -Xaa3.
European Published Patent Application 0528486 discloses cysteine containing tetrapeptide amides inhibitors of PFT of the Formula Cys-Xaa1 -Xaa2 -Xaa3 -NRR1.
European Published Patent Application 0535730 discloses pseudotetrapeptide inhibitors of PFT of the following two formulas: ##STR2##
Copending U.S. patent application Ser. No. 08/268,364 discloses a series of histidine and homohistidine derivatives as inhibitors of protein farnesyltransferase.
U.S. Pat. No. 4,870,183 discloses a series of amino acid derivatives of the Formula I: ##STR3## wherein Ar represents a phenyl group, a naphthyl group, or pyridyl group which may have a substituent. His represents an L-histidyl group, Y represents --O-- or --NH--, R represents a straight or branched chain alkyl group, a cycloalkyl group or a halogenated alkyl group, or pharmaceutically acceptable acid addition salts thereof, useful for treatment of hypertension, especially renin-associated hypertension. Additionally, U.S. Pat. No. 4,870,183 discloses intermediates of Formula IV: ##STR4## wherein the carbon atom marked with (S) is of S-- configuration and Ar is as defined above which are used to prepare compounds of Formula I.
U.S. Pat. No. 4,904,660 discloses a series of histidine derivatives useful as renin inhibitors in the treatment of hypertension. Additionally, U.S. Pat. No. 4,904,660 discloses intermediates of formula ##STR5## wherein R1 is ##STR6## and R2 is ##STR7## which are used to prepare the target renin inhibitors.
Compounds disclosed in the above references do not disclose or suggest the novel combination of structural variations found in the present invention described hereinafter.
We have surprisingly and unexpectedly found that a series of substituted histidines are inhibitors of farnesyltransferase and thus useful as agents for the treatment of proliferative diseases such as, for example, cancer, restenosis, and psoriasis, and as antiviral agents.
SUMMARY OF THE INVENTION
A compound of Formula I ##STR8## wherein R is hydrogen or alkyl; R1 and R2 may be the same or different and are selected from the group consisting of: ##STR9## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and R7 may be 1 to 3 substituents selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkoxy,
thioalkoxy,
hydroxy,
mercapto,
halogen,
nitro, ##STR10## wherein R8 and R9 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl, or R8 and R9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is hydrogen or alkyl, and
n is zero or an integer of one to four, ##STR11## wherein R9a is hydrogen or alkyl and R8, R9, and n are as defined above, and ##STR12## wherein R11 is selected from the group consisting of: hydrogen,
alkyl, and
aryl, ##STR13## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
NR12 wherein R12 is hydrogen, alkyl, or ##STR14## wherein R8, R9, and n are as defined above, or R12 is absent,
O,
S,
SO, and
SO2, and
Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH2 unit, and R12 is absent, and R7 is as defined above, ##STR15## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R7 are as defined above, and R12 may be present, ##STR16## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R7 are as defined above, and R12 may be present, ##STR17## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R7 is as defined above with the proviso that R1 and R2 are not both a monocyclic ring, and ##STR18## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R7 and Z are as defined above with the proviso that R1 and R2 are not both a monocyclic ring;
R3 is hydrogen or alkyl;
R4 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
benzyl,
alkyl chain wherein the alkyl chain may be interrupted by a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above, ##STR19## wherein p is an integer of one to four, and R13 is alkyl or benzyl, and ##STR20## wherein p and R13 are as defined above; X is ##STR21## Y is ##STR22## R5 is selected from the group consisting of: --OR14 wherein R14 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
hydroxyalkyl,
mercaptoalkyl,
cyanoalkyl,
nitroalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR23## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
HO2 C-alkyl,
alkyl-O2 C-alkyl, and ##STR24## wherein R15 and R16 are as defined above, --S--R14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, ##STR25## wherein R17 and R18 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cyanoalkyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
mercaptoalkyl,
nitroalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR26## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
or R17 and R18 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
--NH--OR10 wherein R10 is as defined above,
alkyl,
alkenyl, and
arylalkyl; and
R6 is hydrogen,
--SR where R is as defined above,
--OR where R is as defined above, or ##STR27## wherein R and Ra may be the same or different and are as defined above for R;
and when X is --CH2 -- and R17 is hydrogen or alkyl then
R18 may be ##STR28## wherein R is as defined above, or ##STR29## wherein R is as defined above; and when X is ##STR30## must be ##STR31## and excluding the compound wherein
R is hydrogen,
R1 and R2 are each ##STR32## R3 is hydrogen, R4 is hydrogen, ##STR33## R5 is OR14 wherein R14 is hydrogen, and R6 is hydrogen;
and corresponding isomers thereof;
or a pharmaceutically acceptable salt thereof.
As inhibitors of farnesyltransferase, the compounds of Formula I are antiproliferative agents. Thus, they are useful for the treatment of cancer, restenosis, and psoriasis, and as antiviral agents. Additionally, a compound of Formula I may be combined with other conventional anti-cancer agents such as, for example, cisplatin.
A still further embodiment of the present invention is a pharmaceutical composition for administering an effective amount of a compound of Formula I in unit dosage form in the treatment methods mentioned above. Finally, the present invention is directed to methods for production of a compound of Formula I.
DETAILED DESCRIPTION OF THE INVENTION
In the compounds of Formula I, the term "alkyl" means a straight or branched hydrocarbon radical having from 1 to 12 carbon atoms and includes, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, undecyl, dodecyl, and the like.
The term "alkenyl" means a straight or branched unsaturated hydrocarbon radical having from 2 to 12 carbon atoms and includes, for example, ethenyl, 2-propenyl, 1-butenyl, 2-butenyl, 1-pentenyl, 2-pentenyl, 3-methyl-3-butenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 3-heptenyl, 1-octenyl, 1-nonenyl, 1-decenyl, 1-undecenyl, 1-dodecenyl, and the like.
The term "alkynyl" means a straight or branched triple bonded unsaturated hydrocarbon radical having from 2 to 12 carbon atoms and includes, for example, ethynyl, 2-propynyl, 3-butynyl, 4-pentynyl, 5-hexynyl, 6-heptynyl, 7-octynyl, 8-nonynyl, 9-decynyl, 10-undecynyl, 11-dodecynyl, and the like.
The term "cycloalkyl" means a saturated hydrocarbon ring which contains from 3 to 12 carbon atoms, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, adamantyl, and the like.
The term "cycloalkylalkyl" means a saturated hydrocarbon ring attached to an alkyl group wherein alkyl is as defined above. The saturated hydrocarbon ring contains from 3 to 12 carbon atoms. Examples of such are cyclopropylmethyl, cyclopentylmethyl, cyclohexylmethyl, adamantylmethyl and the like.
The terms "alkoxy" and "thioalkoxy" are O-alkyl or S-alkyl as defined above for alkyl.
The term "aryl" means an aromatic radical which is a phenyl group, a naphthyl group, a phenyl group substituted by 1 to 4 substituents selected from alkyl as defined above, alkoxy as defined above, thioalkoxy as defined above, hydroxy, halogen, trifluoromethyl, amino, alkylamino as defined above for alkyl, dialkylamino as defined for alkyl, N-acetylamino, cyano --SO2 NH2, or nitro, or a naphthyl group substituted by 1 to 4 substituents as defined above for a phenyl group substituted by 1 to 4 substituents.
The term "heteroaryl" means a heteroaromatic radical which is 2- or 3-thienyl; 2- or 3-furanyl; 1-, 2- or 3-pyrrolyl; 1-, 2-, 4-, or 5-imidazolyl; 1-, 3-, 4-, or 5-pyrazolyl; 2-, 4-, or 5-thiazolyl; 3-, 4-, or 5-isothiazolyl; 2-, 4-, or 5-oxazolyl; 3-, 4-, or 5-isoxazolyl; 1-, 3-, or 5-1,2,4-triazolyl; 1-, 2-, 4-, or 5-1,2,3-triazolyl; 1- or 5-tetrazolyl; 4-, or 5-1,2,3-oxadiazolyl; 3-, or 5-1,2,4-oxadiazolyl; 2-1,3,4-oxadiazolyl; 2-1,3,4-thiadiazoyl; 2-1,3,5-triazinyl; 3-pyridinyl; 3-, 4-, or 5-pyridazinyl; 2-pyrazinyl; 2-, 4-, or 5-pyrimidinyl; unsubstituted or substituted by 1 to 2 substituents selected from NH2, OH, S, halogen as defined hereinafter, alkyl as defined above, or alkoxy as defined above.
The term "arylalkyl" means an aromatic radical attached to an alkyl radical wherein aryl and alkyl are as defined above, for example, benzyl, naphthylmethyl, 4-sulfamoylphenylmethyl and the like.
The term "heteroarylalkyl" means a heteroaromatic radical attached to an alkyl radical wherein heteroaryl and alkyl are as defined above, for example, 2-imidazol-1-yl-ethyl, 3-imidazol-1-yl-propyl, 2-(1H-imidazol-4-yl)-ethyl, 2-butyl-1H-imidazol-4-ylmethyl, 2-thiophen-2-yl-ethyl, thiophen-3-ylmethyl, and the like.
"Halogen" is fluorine, chlorine, bromine, or iodine.
The term "haloalkyl" means a halogen atom attached to an alkyl radical wherein halogen and alkyl are as defined above.
The term "hydroxyalkyl" means a hydroxy group attached to an alkyl radical wherein alkyl is as defined above.
The term "mercaptoalkyl" means a mercapto group attached to an alkyl radical wherein alkyl is as defined above.
The term "cyanoalkyl" means a cyano group attached to an alkyl radical wherein alkyl is as defined above.
The term "nitroalkyl" means a nitro group attached to an alkyl radical wherein alkyl is as defined above.
The term "alkoxyalkyl" means an alkoxy group attached to an alkyl radical wherein alkoxy and alkyl are as defined above.
The term "thioalkoxyalkyl" means a thioalkoxy group attached to an alkyl radical wherein thioalkoxy and alkyl are as defined above.
The term "acetamidoalkyl" means a ##STR34## group attached to an alkyl radical wherein alkyl is as defined above.
The term "benzyloxyalkyl" means a benzyloxy group attached to an alkyl radical wherein alkyl is as defined above.
The ring designated: ##STR35## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR36## and the like mono- to trisubstituted by R7 which may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms and unsaturated carbon atoms wherein the --CH2 -- group is attached, and R7 is selected from hydrogen, alkyl, alkenyl, alkoxy, thioalkoxy, hydroxy, mercapto, halogen, nitro, ##STR37## wherein R8 and R9 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl, or
R8 and R9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is hydrogen or alkyl, n is zero or an integer of one to four, ##STR38## wherein R9a is hydrogen or alkyl and R8, R9, and n are as defined above, and ##STR39## wherein R11 is selected from the group consisting of hydrogen, alkyl, and aryl wherein alkyl, alkenyl, alkoxy, thioalkoxy, halogen, aryl, and R7 are as defined above.
The ring designated: ##STR40## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, wherein Z is selected from the group consisting of: NR12 wherein R12 is hydrogen or alkyl or ##STR41## wherein R8, R9, and n are as defined above, or R12 is absent, O, S, SO, and SO2 and Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring system is aromatic, Z is not at the point of attachment of the CH2 -- unit, and R12 is absent, for example: ##STR42## and the like mono- to trisubstituted by R7 as defined above wherein R7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms and unsaturated carbon atoms wherein the --CH2 -- group is attached.
The ring designated: ##STR43## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR44## and the like mono- and trisubstituted by R7 as defined above wherein R7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms, and unsaturated carbon atoms wherein the --CH2 -- group is attached, and Z is as defined above and R12 may be present.
The ring designated: ##STR45## represents a bicyclic ring that may be either aromatic, or partially or completely saturated, for example: ##STR46## and the like mono- and trisubstituted by R7 as defined above wherein R7 may be attached at any carbon atom of the bicyclic ring excluding unsaturated bridgehead carbon atoms, and unsaturated carbon atoms wherein the --CH2 -- is attached, and Z is as defined above and R12 may be present.
The ring designated: ##STR47## wherein the monocyclic ring may be aromatic, or partially or completely saturated, for example: ##STR48## and the like mono- to trisubstituted by R7 as defined above wherein R7 may be attached at any carbon atom of the monocyclic ring excluding unsaturated carbon atoms wherein the --CH2 -- group is attached.
The ring designated: ##STR49## wherein the monocyclic ring may be aromatic, or partially or completely saturated, for example: ##STR50## and the like mono- to trisubstituted by R7 as defined above wherein R7 may be attached at any carbon atom of the monocyclic ring excluding unsaturated carbon atoms wherein the --CH2 -- group is attached.
The compounds of Formula I are capable of further forming both pharmaceutically acceptable acid addition and/or base salts. All of these forms are within the scope of the present invention.
Pharmaceutically acceptable acid addition salts of the compounds of Formula I include salts derived from nontoxic inorganic acids such as hydrochloric, nitric, phosphoric, sulfuric, hydrobromic, hydriodic, hydrofluoric, phosphorous, and the like, as well as the salts derived from nontoxic organic acids, such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids, aliphatic and aromatic sulfonic acids, etc. Such salts thus include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, nitrate, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, trifluoroacetate, propionate, caprylate, isobutyrate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, mandelate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, phthalate, benzenesulfonate, toluenesulfonate, phenylacetate, citrate, lactate, maleate, tartrate, methanesulfonate, and the like. Also contemplated are salts of amino acids such as arginate and the like and gluconate, galacturonate (see, for example, Berge S. M., et al., "Pharmaceutical Salts," J. of Pharma. Sci., 66:1 (1977)).
The acid addition salts of said basic compounds are prepared by contacting the free base form with a sufficient amount of the desired acid to produce the salt in the conventional manner. The free base form may be regenerated by contacting the salt form with a base and isolating the free base in the conventional manner. The free base forms differ from their respective salt forms somewhat in certain physical properties such as solubility in polar solvents, but otherwise the salts are equivalent to their respective free base for purposes of the present invention.
Pharmaceutically acceptable base addition salts are formed with metals or amines, such as alkali and alkaline earth metals or organic amines. Examples of metals used as cations are sodium, potassium, magnesium, calcium, and the like. Examples of suitable amines are N,N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, dicyclohexylamine, ethylenediamine, N-methylglucamine, and procaine (see, for example, Berge S. M., et al., "Pharmaceutical Salts," J. of Pharma. Sci., 66:1 (1977)).
The base addition salts of said acidic compounds are prepared by contacting the free acid form with a sufficient amount of the desired base to produce the salt in the conventional manner. The free acid form may be regenerated by contacting the salt form with an acid and isolating the free acid in the conventional manner. The free acid forms differ from their respective salt forms somewhat in certain physical properties such as solubility in polar solvents, but otherwise the salts are equivalent to their respective free acid for purposes of the present invention.
Certain of the compounds of the present invention can exist in unsolvated forms as well as solvated forms, including hydrated forms. In general, the solvated forms, including hydrated forms, are equivalent to unsolvated forms and are intended to be encompassed within the scope of the present invention.
Certain of the compounds of the present invention possess one or more chiral centers and each center may exist in the R(D) or S(L) configuration. The present invention includes all enantiomeric and epimeric forms as well as the appropriate mixtures thereof.
A preferred compound of Formula I is one wherein
R is hydrogen;
R7 is selected from the group consisting of:
hydrogen,
methoxy,
thiomethoxy,
hydroxy,
halogen, and ##STR51## wherein R8 and R9 may be the same or different and are selected from the group consisting of:
hydrogen, and
alkyl;
R3 is hydrogen or methyl;
R4 is selected from the group consisting of:
hydrogen,
methyl,
ethyl, and
--CH2 --O--CH3 ;
X is ##STR52## or Y is ##STR53## or R5 is selected from the group consisting of:
--O--R14 wherein R14 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
hydroxyalkyl,
mercaptoalkyl,
cyanoalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR54## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: O, and NR10 wherein R10 is hydrogen or methyl, and
alkyl-O2 C-alkyl,
--S--R14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, and ##STR55## wherein R19 is hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
cyanoalkyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
mercaptoalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR56## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above, and
R20 is hydrogen or methyl; and
R6 is hydrogen.
A more preferred compound of Formula I is one wherein R1 and R2 may be the same or different and are selected from the group consisting of: ##STR57## R3 is hydrogen or methyl; R4 is hydrogen, methyl, or --CH2 OCH3 ;
X is --CH2 -- or ##STR58## and R5 is selected from the group consisting of:
--O--R14 wherein R14 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl, ##STR59## --SR14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, and ##STR60## wherein R17 is selected from the group consisting of: ##STR61## R18 is hydrogen or methyl, and --NH--OR10 wherein R10 is hydrogen or methyl.
Particularly valuable is a compound selected from the group consisting of:
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid;
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, ethyl ester;
(S)-3-(3-Methyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, propyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, isopropyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, butyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, benzyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclohexyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclopropylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-butyl-1H-imidazol-4-ylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, (±)-sec-butyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, allyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, prop-2-ynyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-cyano-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-benzyloxy-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-thiophen-2-yl-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, thiophen-3-ylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-diethylamino-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-morpholin-4-yl-ethyl ester;
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(Carbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-iraidazol-1-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(2-Ethylsulfanyl-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-[2-(1H-imidazol-4-yl)-ethylcarbamoyl]-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(3-imidazol-1-yl)-propylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(2-Hydroxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-isopropylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-morpholin-4-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(2-Diethylamino-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-methylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-Ethylcarbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-{2-(1H-Imidazol-4-yl)-1-[2-(4-sulfamoyl-phenyl)-ethylcarbamoyl]-ethyl}-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, methyl ester;
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionamide;
(S)-2-(3-Benzo[b]thiophen-3-yl-2-benzo[b]thiophen-3-ylmethyl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-thiopropionic acid, S-(2-acetylamino-ethyl) ester;
(S)-N-[1-(2-Cyano-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-hydroxy-ethyl ester;
(S)-N-[1-Dimethylcarbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-ynyl ester;
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, 2-cyano-ethyl ester;
(S)-N-[2-(1H-Imidazol-4-yl)-1-propylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-imidazol-1-yl-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-enyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, 2-cyano-ethyl ester;
(S)-N-[2-(1H-Imidazol-4-yl)-1-phenethylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-[methyl-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propiony)-amino]-propionic acid, methyl ester;
(S)-N-[1-Hydroxymethyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-[1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, phenethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 3-cyano-propyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 3-methyl-but-2-enyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methoxycarbonylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyanomethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-(2-hydroxy-ethyldisulfanyl)-ethyl ester;
(S)-3-(3-Methoxymethyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 1-cyano-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propan-1-ol;
(S)-3-(1H-Imidazol-4-yl)-N-methyl-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionamide;
(S)-N-[1-Methylcarbamoyl-2-(3-methyl-3H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide; and
(S)-2-(2-Benzyl-3-naphthalen-1-yl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester; and corresponding isomers thereof; or a pharmaceutically acceptable salt thereof.
The compounds of Formula I are valuable inhibitors of the enzyme farnesyltransferase.
The protein:farnesyltransferase (PFT) or farnesyl protein transferase (FPT) inhibitory activity of compounds of Formula I was assayed in HEPES buffer (pH 7.4) containing 5 mM potassium phosphate and 20 μM ZnCl2. The solution also contained 7 mM DTT, 1.2 mM MgCl2. Assays were performed in 96 well plates (Wallec) and employed solutions composed of varying concentrations of a compound of Formula I in 100% DMSO. Upon addition of both substrates, radiolabeled farnesyl pyrophosphate ([1-3 H], specific activity 15-30 Ci/mmol, final concentration 0.12 μM) and (biotinyl)-Ahe-Tyr-Lys-Cys-Val-Ile-Met ([3aS[3a alpha, 4 beta, 6a alpha]-hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-5-pentanoic acid]-[7-aminoheptanoic acid]-Thr-Lys-Cys-Val-Ile-Met) (final concentration 0.2 μM), the enzyme reaction was started by addition of 40-fold purified rat brain farnesyl protein transferase. After incubation at 37° C. for 30 minutes, the reaction was terminated by diluting the reaction 2.5-fold with a stop buffer containing 1.5 M magnesium acetate, 0.2 M H3 PO4, 0.5% BSA, and strepavidin beads (Amersham) at a concentration of 1.3 mg/mL. After allowing the plate to settle for 30 minutes at room temperature, radioactivity was quantitated on a microBeta counter (Model 1450, Wallec). Compounds of Formula I show IC50 values of 0.06 to 60 μM in this assay and are thus valuable inhibitors of protein:farnesyltransferase enzyme which may be used in the medical treatment of tissue proliferative diseases, including cancer and restenosis. The assay was also carried out without 5 mM potassium phosphate.
Gel Shift Assay
Twenty-four hours after planting 2×106 ras-transformed cells per treatment condition, the farnesylation inhibitor is added at varying concentrations. Following an 18-hour incubation period, cells are lysed in phosphate-buffered saline containing 1% Triton X-100, 0.5% sodium deoxycholate, and 0.1% SDS, pH 7.4 in the presence of several protease inhibitors (PMSF, antipain, leupeptin, pepstatin A, and aprotinin all at 1 μg/mL). Ras protein is immunoprecipitated from the supernatants by the addition of 3 μg v-H-ras Ab-2 (Y13-259 antibody from Oncogene Science). After overnight immunoprecipitation, 30 μL of a 50% protein G-Sepharose slurry (Pharmacia) is added followed by 45-minute incubation. Pellets are resuspended in 2× tris-glycine loading buffer (Novex) containing 5% B-mercaptoethanol and then denatured by 5 minutes boiling prior to electrophoresis on 14% Tris-glycine SDS gels. Using Western transfer techniques, proteins are transferred to nitrocellulose membranes followed by blocking in blocking buffer. Upon overnight incubation with primary antibody (pan-ras Ab-2 from Oncogene Science), an antimouse HRP conjugate secondary antibody (Amersham) is employed for detection of the ras protein. Blots are developed using ECL techniques (Amersham).
The data in Table 1 show farnesyl protein transferase inhibitory activity, and activity in the gel shift assay against ras protein of selected compounds of Formula I.
                                  TABLE 1                                 
__________________________________________________________________________
Biological Activity of Compounds of Formula I                             
                                  Inhibition of Ras                       
  Farnesyl Protein Farnesylation                                          
  Transferase Inhibition (Minimum Effective Dose, μM)                  
                     Hepes                                                
                         Hepes/5 mM PO.sub.4.sup.-3                       
                                  H-ras  H-ras-                           
    IC.sub.50 IC.sub.50 Me 12/+ NIH3T3                                    
  Example Compound (μM) (μM) Cells Cells                            
__________________________________________________________________________
 1   (S)-3-(1H-Imidazol-4-yl)-2-(3-                                       
                     0.26                                                 
                         0.30     2.5    5.0                              
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, methyl ester                                                     
   2 (R)-3-(1H-Imidazol-4-yl)-2-(3- 12 10                                 
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl propionylamino)-propionic                                     
   acid, methyl ester                                                     
   3 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.0 2.3                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid                                                                   
   4 (R)-3-(1H-Imidazol-4-yl)-2-(3- 6.1 19.5                              
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid                                                                   
   5 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.13 0.13 2.5                         
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, ethyl ester                                                      
   6 (S)-3-(3-Methyl-3H-imidazol-4-yl)- 0.42 0.22 1 2.5                   
   2-(3-naphthalen-1-yl-2-naphthalen-                                     
   1-ylmethyl-propionylamino)-                                            
   propionic acid, methyl ester                                           
   7 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.11 0.10 5.0                         
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, propyl ester                                                     
   8 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.18 0.16 5.0 5.0                     
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, isopropyl ester                                                  
   9 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.3 1.1                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, butyl ester                                                      
  10 (S)-3-(1H-Imidazol-4-yl)-2-(3- 4.2 2.9                               
   naphthalen-1-yl-2-naphthalen-1                                         
   ylmethyl-propionylamino)-propionic                                     
   acid, benzyl ester                                                     
  11 (S)-3-(1H-Imidazol-4-yl)-2-(3- 20 14                                 
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, cyclohexyl ester                                                 
  12 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.40 0.25                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, cyclopropylmethyl ester                                          
  13 (S)-3-(1H-Imidazol-4-yl)-2-(3- 2.1 1.7                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-butyl-1H-imidazol-4-                                           
   ylmethyl ester                                                         
  14 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.82 0.54                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, ±-sec-butyl ester                                             
  15 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.40 0.20  25.0                       
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, allyl ester                                                      
  16 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.50 0.59                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, prop-2-ynyl ester                                                
  17 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.08 0.07  10                         
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-cyanoethyl ester                                               
  18 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.57 0.54                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-benzyloxyethyl ester                                           
  19 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.1 1.1                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-thiophen-2-yl-ethyl ester                                      
  20 (S)-3-(1H-Imidazol-4-yl)-2-(3- 3.5 2.4                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, thiophen-3-ylmethyl ester                                        
  21 (S)-3-(1H-Imidazol-4-yl)-2-(3- 21 9.0                                
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-diethylamino-ethyl ester                                       
  22 (S)-3-(1H-Imidazol-4-yl)-2-(3- 5.8 4.3                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-morpholin-4-yl-ethyl ester                                     
  23 (S)-N-[1-(2-Benzyloxy- 0.74 0.55 5.0                                 
   ethylcarbamoyl)-2-(1H-imidazol-4-                                      
   yl)-ethyl]-3-naphthalen-1-yl-2-                                        
   naphthalen-1-ylmethyl-propionamide                                     
  24 (S)-N-[1-Carbamoyl-2-(1H-imidazol- 5.3 3.8                           
   4-yl)-ethyl]-3-naphthalen-1-yl-2-                                      
   naphthalen-1-ylmethyl-propionamide                                     
  25 (S)-N-[2-(1H-Imidazol-4-yl)-1-(2- 1.9 1.5                            
   imidazol-1-yl-ethylcarbamoyl)-                                         
   ethyl]-3-naphthalen-1-yl-                                              
   2-naphthalen-1-ylmethyl-                                               
   propionamide                                                           
  26 (S)-N-[1-(2-Ethylsulfanyl- 12 8.7                                    
   ethylcarbamoyl)-2-(1H-imidazol-4-                                      
   yl)-ethyl]-3-naphthalen-1-yl-2-                                        
   naphthalen-1-ylmethyl-propionamide                                     
  27 (S)-N-{2-(1H-Imidazol-4-yl)-1-[2- 4.8 3.9 10.0                       
   (1H-imidazol-4-yl)-                                                    
   ethylcarbamoyl]-ethyl}-3-                                              
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  28 (S)-N-[2-(1H-Imidazol-4-yl)-1-(3- 8.1 4.2                            
   imidazol-1-yl)-propylcarbamoyl)-                                       
   ethyl]-3-naphthalen-1-yl-2-                                            
   naphthalen-1-ylmethyl-propionamide                                     
  29 (S)-N-[1-(2-Hydroxy- 3.2 3.7                                         
   ethylcarbamoyl)-2-(1H-imidazol-4-                                      
   yl)-ethyl]-3-naphthalen-1-yl-2-                                        
   naphthalen-1-ylmethyl-propionamide                                     
  30 (S)-N-[2-(1H-Imidazol-4-yl)-1- 4.6 3.5                               
   isopropylcarbamoyl-ethyl]-3-                                           
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  31 (S)-N-[2-(1H-Imidazol-4-yl)-1-(2- 26 15                              
   morpholin-4-yl-ethylcarbamoyl)-                                        
   ethyl]-3-naphthalen-1-yl-2-                                            
   naphthalen-1-ylmethyl-propionamide                                     
  32 (S)-N-[1-(2-Diethylamino- 19 14                                      
   ethylcarbamoyl)-2-(1H-imidazol-4-                                      
   yl)-ethyl]-(3-naphthalen-1-yl-2-                                       
   naphthalen-1-ylmethyl-propionamide                                     
  33 (S)-N-[2-(1H-Imidazol-4-yl)-1- 0.61 0.44 1.0 1.0                     
   methylcarbamoyl-ethyl]-3-                                              
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  34 (S)-N-[1-Ethylcarbamoyl-2-(1H- 2.9 2.0                               
   imidazol-4-yl)-ethyl]-3-                                               
   naphthalen-1-yl-2-naphthalene-1-                                       
   ylmethyl-propionamide                                                  
  35 (S)-N-{2-(1H-Imidazol-4-yl)-1-[2- 15 14                              
   (4-sulfamoyl-phenyl)-                                                  
   ethylcarbamoyl]-ethyl}-3-                                              
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  36 (S)-2-[3-(Decahydro-naphthalen-1- 0.31 0.37 25.0                     
   yl)-2-(decahydro-naphthalen-1-                                         
   ylmethyl)-propionylamino]-3-(1H-                                       
   imidazol-4-yl)-propionic acid,                                         
   methyl ester                                                           
  37 (S)-3-(1H-Imidazol-4-yl)-2-[3- 0.42 0.18 10.0                        
   (5,6,7,8-tetrahydro-naphthalen-1-                                      
   yl)-2-(5,6,7,8-tetrahydro-                                             
   naphthalen-1-ylmethyl)-                                                
   propionylamino]-propionic acid,                                        
   methyl ester                                                           
  38 (S)-N-[1-(2-Benzyloxy- 0.78 0.85                                     
   ethylcarbamoyl)-2-(1H-imidazol-4-                                      
   yl)-ethyl]-3-(5,6,7,8-tetrahydro-                                      
   naphthalen-1-yl)-2-(5,6,7,8-                                           
   tetrahydro-naphthalen-1-ylmethyl)-                                     
   propionamide                                                           
  39 (S)-2-(3-Benzo[b]thiophen-3-yl-2- 1.2 0.97                           
   benzo[b]thiophen-3-ylmethyl-                                           
   propionylamino)-3-(1H-imidazol-4-                                      
   yl)-propionic acid, methyl ester                                       
  40 (S)-3-(1H-Imidazol-4-yl)-2-(3- 2.6 3.2                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-                                              
   thiopropionic acid, S-(2-                                              
   acetylamino-ethyl)ester                                                
  41 (S)-N-[1-(2-Cyano-ethylcarbamoyl)- 0.97 0.71  5                      
   2-(1H-imidazol-4-yl)-ethyl]-3-                                         
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  42 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.8 1.3                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-hydroxy-ethyl ester,                                           
   trifluoroacetate salt                                                  
  43 (S)-N-[1-Dimethylcarbamoyl-2-(1H- 5.6 3.8                            
   imidazol-4-yl)-ethyl]-3-                                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  44 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.23 0.21                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, but-3-ynyl ester,                                                
   trifluoroacetate salt                                                  
  45 (S)-2-[3-(Decahydro-naphthalen-1- 0.49 0.32                          
   yl)-2-(decahydro-naphthalen-1-                                         
   ylmethyl)-propionylamino]-3-(1H-                                       
   imidazol-4-yl)-propionic acid,                                         
   2-cyano-ethyl ester                                                    
  46 (S)-N-(2-(1H-Imidazol-4-yl)-1- 4.9 5.6                               
   propylcarbamoyl-ethyl]-3-                                              
   naphthalen-1-yl)-2-naphthalen-1-                                       
   ylmethyl)-propionamide                                                 
  47 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.1 1.4                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-imidazol-1-yl-ethyl ester                                      
  48 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.2 1.3                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, but-3-enyl ester                                                 
  49 (S)-3-(1H-Imidazol-4-yl)-2-[3- 0.29 0.37                             
   (5,6,7,8-tetrahydro-naphthalen-1-                                      
   yl)-2-(5,6,7,8-tetrahydro-                                             
   naphthalen-1-ylmethyl)-                                                
   propionylamino]-propionic acid,                                        
   2-cyano-ethyl ester                                                    
  50 (S)-N-[2-(1H-Imidazol-4-yl)-1- 4.8 6.0                               
   phenethylcarbamoyl-ethyl]-3-                                           
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl)-propionamide                                                 
  51 (S)-3-(1H-Imidazol-4-yl)-2- 2.0 2.2  5                               
   [methyl-(3-naphthalen-1-yl-2-                                          
   naphthalen-1-ylmethyl-propionyl)-                                      
   amino]-propionic acid, methyl                                          
   ester, trifluoroacetate salt                                           
  52 (S)-N-[1-Hydroxymethyl-2-(1H- 3.0 3.4  25                            
   imidazol-4-yl)-ethyl)-3-                                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  53 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.07 0.06  25                         
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, phenethyl ester                                                  
  54 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.44 0.37                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 3-cyano-propyl ester                                             
  55 (S)-3-(1H-Imidazol-4-yl)-2-(3- 5.3 4.3                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 3-methyl-but-2-enyl ester                                        
  56 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.37 0.36                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propylamino)-propionic                                        
   acid, methyl ester                                                     
  57 (S)-3-(1H-Imidazol-4-yl)-2-(3- 5.5 4.1                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, methoxycarbonylmethyl ester                                      
  58 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.40 0.51                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, cyanomethyl ester                                                
  59 (S)-3-(1H-Imidazol-4-yl)-2-(3- 1.3 1.0                               
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 2-(2-hydroxy-ethyl-                                              
   disulfanyl)-ethyl ester                                                
  60 (S)-3-(3-Methoxymethyl-3H- 0.93 1.0                                  
   imidazol-4-yl)-2-(3-naphthalen-1-                                      
   yl-2-naphthalen-1-ylmethyl-                                            
   propionylamino)-propionic acid,                                        
   methyl ester                                                           
  61 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.64 0.61                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionylamino)-propionic                                     
   acid, 1-cyano-ethyl ester                                              
  62 (S)-3-(1H-Imidazol-4-yl)-2-(3- 0.55 0.50                             
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propylamino)-propan-1-ol                                      
  63 (S)-3-(1H-Imidazol-4-yl)-N-methyl- 0.20 0.24                         
   2-(3-naphthalen-1-yl-2-naphthalen-                                     
   1-ylmethyl-propylamino)-                                               
   propionamide                                                           
  64 (S)-N-[1-Methylcarbamoyl-2-(3- 0.61 0.46                             
   methyl-3H-imidazol-4-yl)-ethyl]-3-                                     
   naphthalen-1-yl-2-naphthalen-1-                                        
   ylmethyl-propionamide                                                  
  65 (S)-2-(2-Benzyl-3-naphthalen-1-yl- 1.6 2.9  25                       
   propionylamino)-3-(1H-imidazol-4-                                      
   yl)-propionic acid, methyl ester                                       
__________________________________________________________________________
A compound of Formula Ia ##STR62## wherein R is hydrogen or alkyl; R1 and R2 may be the same or different and are selected from the group consisting of: ##STR63## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and R7 may be 1 to 3 substituents selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkoxy,
thioalkoxy,
hydroxy,
mercapto,
halogen,
nitro, ##STR64## wherein R8 and R9 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl, or R8 and R9 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of:
S, O, and N--R10 wherein R10 is hydrogen or alkyl, and
n is zero or an integer of one to four, ##STR65## wherein R9a is hydrogen or alkyl and R8, R9, and n are as defined above, and ##STR66## wherein R11 is selected from the group consisting of: hydrogen,
alkyl, and
aryl, ##STR67## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
NR12 wherein R12 is hydrogen, alkyl, or ##STR68## wherein R8, R9, and n are as defined above, or R12 is absent,
O,
S,
SO, and
SO2, and
Z may be at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH2 unit and R12 is absent, and R7 is as defined above, ##STR69## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R7 are as defined above and R12 may be present, ##STR70## wherein the bicyclic ring may be aromatic, or partially or completely saturated, and Z and R7 are as defined above, and R12 may be present, ##STR71## wherein the monocyclic may be aromatic, or partially or completely saturated, and R7 is as defined above with the proviso that R1 and R2 are not both a monocyclic ring, and ##STR72## wherein the monocyclic ring may be aromatic, or partially or completely saturated, and R7 and Z are as defined above with the proviso that R1 and R2 are not both a monocyclic ring;
R3 is hydrogen or alkyl;
Xa is ##STR73## Y is ##STR74## R5a is selected from the group consisting of: --OR14a wherein R14a is selected from the group consisting of:
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
hydroxyalkyl,
mercaptoalkyl,
cyanoalkyl,
nitroalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR75## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
HO2 C-alkyl,
alkyl-O2 C-alkyl, and ##STR76## wherein R15 and R16 are as defined above, --S--R14a wherein R14a is as defined above; ##STR77## wherein R17 and R18 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cyanoalkyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
mercaptoalkyl,
nitroalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR78## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
or R17 and R18 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
--NH--OR10 wherein R10 is as defined above,
alkyl,
alkenyl, and
arylalkyl; and
R6 is hydrogen,
--SR where R is as defined above,
--OR where R is as defined above, or ##STR79## wherein R and Ra may be the same or different and are as defined above for R; and
corresponding isomers thereof; or a pharmaceutically acceptable salt thereof may be prepared by reacting a compound of Formula II ##STR80## wherein Trt is (C6 H5)3 --C-- and R, R1, R2, R3, Xa, Y, R5a and R6 are as defined above with an acid such as, for example, 80% to 85% acetic acid and the like at about 90° C. for about 0.5 hours to afford a compound of Formula Ia. Preferably, the reaction is carried out with 80% to 85% acetic aced at about 90° C. for about 0.5 hours.
A compound of Formula Ib ##STR81## wherein R5b is OH and R, R1, R2, R3, Xa, and R6 as defined above may be prepared by reacting a compound of Formula Ic ##STR82## wherein R5c is OCH3 or SCH3 and R, R1, R2, R3, Xa, R6 and Trt are as defined above with a base such as, for example, 1N sodium hydroxide and the like in a solvent such as, for example, methanol, dioxane, and the like at about room temperature for about 2 hours to afford a compound of Formula Ic-1. ##STR83## wherein R, R1, R2, R3, Xa, R5b, R6, and Trt are as defined above. Preferably, the reaction is carried out with 1N sodium hydroxide in methanol at about room temperature for about 2 hours. A compound of Formula Ic-1 is converted to a compound of Formula Ib using methodology used to prepare a compound of Formula Ia from a compound of Formula II to afford a compound of Formula Ib.
A compound of Formula Id ##STR84## wherein Xb is --CH2 -- and R, R1, R2, R3, R5b, and R6 are as defined above may be prepared by reacting a compound of Formula Ia with a metal hydride such as, for example, lithium borohydride and the like in a solvent such as, for example, tetrahydrofuran and the like at about 0° C. to about room temperature to afford a compound of Formula Id. Preferably, the reaction is carried out with lithium borohydride in tetrahydrofuran at about 25° C.
A compound of Formula Ie ##STR85## wherein R5c-1 is OR14a wherein R14a is selected from the group consisting of:
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
hydroxyalkyl,
mercaptoalkyl,
cyanoalkyl,
nitroalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR86## wherein R15 and R16 may be the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring optionally containing a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
HO2 C-alkyl,
alkyl-O2 C-alkyl, and ##STR87## wherein R15 and R16 are as defined above; and R, R1, R2, R3, Xb, and R6 are as defined above may be prepared by reacting a compound of Formula III ##STR88## wherein R, R1, and R2 are as defined above with a compound of Formula IV ##STR89## wherein Xb, R5c-1, and R6 are as defined above in the presence of a coupling reagent such as, for example, N,N-dicyclohexylcarbodiimide (DCC) and 1-hydroxybenzotriazole (HOBt) and the like in a solvent such as, for example, tetrahydrofuran (THF) at about room temperature for about 12 hours to afford a compound of Formula Ie. Preferably, the reaction is carried out with DCC and HOBt in THF at about room temperature.
A compound of Formula If ##STR90## wherein R17 is ##STR91## R, R1, R2, R3, Xb, and R6 are as defined above may be prepared from a compound of Formula V ##STR92## wherein R, R1, R2, R3, Xb, R6, R17, and Trt are as defined above using methodology used to prepare a compound of Formula Ia from a compound of Formula II to afford a compound of Formula If.
A compound of Formula Ig ##STR93## wherein R5d is alkyl,
alkenyl, and
arylalkyl, and
R, R1, R2, R3, and R6 are as defined above may be prepared by reacting a compound of Formula III with a compound of Formula VI ##STR94## wherein R5d and R6 are as defined above using methodology used to prepare a compound of Formula Ie from a compound of Formula III and a compound of Formula IV to afford a compound of Formula Ig.
A compound of Formula Ih ##STR95## wherein R4a is selected from the group consisting of: alkyl,
alkenyl,
alkynyl,
benzyl,
alkyl chain wherein the alkyl chain may be interrupted by a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is hydrogen or alkyl, ##STR96## wherein p is an integer of one to four, and R13 is alkyl or benzyl, and ##STR97## wherein p and R13 are as defined above, and R, R1, R2, R3, Xa, R5a, and R6 are as defined above may be prepared from a compound of Formula VII ##STR98## wherein R, R1, R2, R3, R4a, R5a, R6, Xa, and Trt are as defined above in the presence of 80% acetic acid to afford a compound of Formula Ih.
A compound of Formula Ii ##STR99## wherein R, R1, R2, R3, R4a, R5a, R6, and Xa are as defined above may be prepared from a compound of Formula Ih in the presence of Lawesson's Reagent (2,4-bis(4-methoxyphenyl)-1,3-dithia-2,4-diphosphetane-2,4-disulfide) and pyridine to afford a compound of Formula Ii
A compound of Formula Ij ##STR100## wherein R, R1, R2, R4a, R5a, R6, and Xa are as defined above by reacting a compound of Formula VIII ##STR101## wherein R, R1, and R2 are as defined above with a compound of Formula IX ##STR102## wherein R4a, R5a, R6, and Xa are as defined above in the presence of a metal hydride such as, for example, sodium cyanoborohydride and the like in the presence of molecular sieves to afford a compound of Formula Ij.
A compound of Formula Ik ##STR103## wherein Y is --CH2 --, ##STR104## R, R1, R2, R4a, R6, and R17 are as defined above may be prepared from a compound of Formula X. ##STR105## wherein R, R1, R2, R4a, R6, and Y are as defined above and a compound of Formula XI
R.sup.17 NH.sub.2                                          XI
wherein R17 is as defined above using the methodology used to prepare a compound of Formula Ij from a compound of Formula VIII and a compound of Formula IX to afford a compound of Formula Ik.
A compound of Formula Il ##STR106## wherein R, R1, R2, R4a, R6, and R17 are as defined above may be prepared from a compound of Formula III and a compound of Formula XII ##STR107## wherein R4a, R6, and R17 are as defined above using methodology used to prepare a compound of Formula Ie from a compound of Formula III and a compound of Formula IV to afford a compound of Formula Il.
A compound of Formula II may be prepared from a compound of Formula XIII ##STR108## wherein R, R1, R2, R3, R6, Y, and Trt are as defined above and a compound of Formula XIV
R.sup.5a H                                                 XIV
wherein R5a is as defined above using standard methodology such as the methodology used to prepare a compound of Formula Ie from a compound of Formula III and a compound of Formula IV to afford a compound of Formula II.
A compound of Formula XIII may be prepared from a compound of Formula XV ##STR109## wherein R, R1, R2, R3, R6, Y, and Trt are as defined above in the presence of a dilute base such as, for example, dilute aqueous sodium hydroxide and the like at room temperature to afford a compound of Formula XIII.
A compound of Formula XVa ##STR110## wherein R, R1, R2, R3, R6, and Trt are as defined above may be prepared by reacting a compound of Formula III and a compound of Formula XVI ##STR111## wherein R3, R6, and Trt are as defined above using methodology used to prepare a compound of Formula Ie from a compound of Formula III and a compound of Formula IV to afford a compound of Formula XVa.
A compound of Formula XVb ##STR112## wherein R, R1, R2, R3, R6, and Trt are as defined above may be prepared from a compound of Formula XVa using the methodology used to prepare compound of Formula Ii from a compound of Formula Ih to afford a compound of Formula XVb.
A compound of Formula XVc ##STR113## wherein R, R1, R2, R3, R6, and Trt are as defined above may be prepared from a compound of Formula VIII and a compound of Formula XVI using the methodology used to prepare a compound of Formula Ij from a compound of Formula VIII and a compound of Formula IX to afford a compound of Formula XVc.
A compound of Formula VIII may be prepared from a compound of Formula XVII ##STR114## wherein R, R1, and R2 are as defined above by treatment with a metal hydride such as, for example, lithium aluminum hydride and the like at about 0° C. to afford a compound of Formula VIII.
A compound of Formula XVII may be prepared from a compound of Formula III and N,0-dimethyl-hydroxylamine in the presence of methyl chloroformate to afford a compound of Formula XVII.
A compound of Formula III may be prepared from a compound of Formula XVIII ##STR115## wherein R, R1, and R2 are as defined above using methodology used to prepare a compound of Formula XIII from a compound of Formula XV to afford a compound of Formula III.
A compound of XVIIIa ##STR116## wherein Ra is alkyl and R1 and R2 are as defined above may be prepared by reacting a compound of Formula XIX ##STR117## wherein R1 and R2 are as defined above with a compound of Formula XX
R.sup.a Br                                                 XX
wherein Ra is as defined above in the presence of a base such as, for example, sodium hydride and the like to afford a compound of Formula XVIIIa.
A compound of Formula XIX may be prepared from a compound of Formula XXI ##STR118## wherein R1 and R2 are as defined above in the presence of about one equivalent of a base such as, for example, sodium hydroxide and the like and a solvent such as, for example, dioxane and the like, and after acidification with an acid such as, for example, hydrochloric acid and the like heating to effect decarboxylation to afford a compound of Formula XIX.
A compound of Formula XXI may be prepared from a compound of Formula XXII ##STR119## wherein R1 is as defined above by reaction with a compound of Formula XXIII
R.sup.2 Br                                                 XXIII
wherein R2 is as defined above using methodology used to prepare a compound of Formula XVIIIa from a compound of Formula XIX and a compound of Formula XX to afford a compound of Formula XXI.
A compound of Formula XXII may be prepared from a dialkylmalonate in the presence of a compound of Formula XXIV
R.sup.1 Br                                                 XXIV
wherein R1 is as defined above using methodology used to prepare a compound of Formula XVIIIa from a compound of Formula XIX and a compound of Formula XX to afford a compound of Formula XXII.
A compound of Formula XXIV may be prepared from a compound of Formula XXV
R.sup.1 OH                                                 XXV
wherein R1 is as defined above by treatment with PBr3 to afford a compound of Formula XXIV.
A compound of Formula XXV may be prepared from a compound of Formula XXVI
R.sup.1a --CO.sub.2 H                                      XXVI
wherein R1a is R1 in which the CH2 group is absent by treatment with a metal hydride such as, for example, lithium aluminum hydride and the like at room temperature to afford a compound of Formula XXV.
A compound of Formula IV may be prepared from a compound of Formula XXVII ##STR120## wherein Boc is tertiary butoxycarbonyl, and R5c-1, R6, and Xb are as defined above by treatment with an acid such as, for example, trifluoroacetic acid and the like in the presence of a solvent such as, for example, dichloromethane and the like to afford a compound of Formula IV.
A compound of Formula XXVII may be prepared from a compound of Formula XXVIII ##STR121## wherein Boc and Xb are as defined above by reaction with a compound of Formula XXIX
R.sup.5c-1 Br                                              XXIX
wherein R5c-1 is as defined above in the presence of a base such as, for example, sodium hydride and the like to afford a compound of Formula XXVII.
A compound of Formula XXVIII may be prepared from a compound of Formula XXX ##STR122## wherein Boc is as defined above by treatment with a metal hydride reagent such as, for example, lithium aluminum hydride and the like in a solvent such as, for example, tetrahydrofuran and the like to afford a compound of Formula XXVIII.
A compound of Formula Va ##STR123## wherein R17a is ##STR124## and R, R1, R2, R3, R6, Xb, and Trt are as defined above may be prepared from a compound of Formula XXXI ##STR125## wherein R, R1, R2, R3, R6, Xb, and Trt are as defined above by reaction with a compound of Formula XXXII
R.sup..sup.17a Cl                                          XXXII
wherein R17a is as defined above in the presence of a base such as, for example, triethylamine and the like to afford a compound of Formula Va.
A compound of Formula XXXI may be prepared from a compound of Formula XXXIII ##STR126## wherein R, R1, R2, R3, R6, and Trt are as defined above by treatment with ammonia in the presence of a metal hydride such as, for example, sodium cyanoborohydride and the like and a solvent such as, for example, 2-propanol and the like to afford a compound of Formula XXXI.
A compound of Formula Vb ##STR127## wherein R17b is ##STR128## and R, R1, R2, R3, R6, Xb, and Trt are as defined above may be prepared from a compound of Formula XXXI by treatment with a compound of Formula XXXIV
R--N═C═O                                           XXXIV
wherein R is as defined above in the presence of a base such as, for example, triethylamine and the like to afford a compound of Formula Vb.
A compound of Formula VI may be prepared from a compound of Formula XXXV ##STR129## wherein R5d, R6, and Trt are as defined above by treatment with 80% acetic acid at about 90° C. for about 0.5 hour to afford a compound of Formula VI.
A compound of Formula XXXV may be prepared from a compound of Formula XXXVI ##STR130## wherein R6 and Trt are as defined above by treatment with about 2 mol of a compound of Formula XXXVII
R.sup.5d Li                                                XXXVII
wherein R5d is as defined above in the presence of a solvent such as, for example, tetrahydrofuran and the like to afford a compound of Formula XXXV.
A compound of Formula VII may be prepared from a compound of Formula XXXVIII ##STR131## wherein R, R1, R2, R3, R5a, R6, Xa, and Trt are as defined above by treatment with a compound of Formula XXXIX
R.sup.4a Br                                                XXXIX
wherein R4a is as defined above in the presence of a solvent such as, for example, dichloromethane and the like to afford a compound of Formula VII.
A compound of Formula IX may be prepared from a compound of Formula XL ##STR132## wherein R6, Boc, and Trt are as defined above by treatment with a compound of Formula XXXIX followed by removal of the Trt group with 80% acetic acid at about 90° C. for about 0.5 hour and subsequent removal of the Boc group with an acid such as, for example, hydrogen chloride gas and the like in a solvent such as, for example, dichloromethane and the like to afford a compound of Formula IX.
A compound of Formula X may be prepared from a compound of Formula XLI ##STR133## wherein R, R1, R2, R4a, R6, and Y are as defined above by treatment with methyl chloroformate, N,O-dimethylhydroxylamine, and piperidine followed by treatment with lithium aluminum hydride in tetrahydrofuran to afford a compound of Formula X.
A compound of Formula XLI may be prepared from a compound of Formula XLII ##STR134## wherein R, R1, R2, R4a, R6, and Y are as defined above by treatment with a dilute base such as, for example, dilute sodium hydroxide and the like to afford a compound of Formula XLI.
A compound of Formula XII may be prepared from a compound of Formula XLIII ##STR135## wherein Boc, R4a, R6, and R17 are as defined above by treatment with an acid such as, for example, hydrogen chloride gas and the like in a solvent such as, for example, dichloromethane and the line to afford a compound of Formula XII.
A compound of Formula XLIII may be prepared from a compound of Formula XLIV ##STR136## wherein Boc, R4a, R6, and R17 are as defined above using the methodology used to prepare a compound of Formula Ii from a compound of Formula Ih to afford a compound of Formula XLIII.
A compound of Formula XLIV may be prepared by reacting a compound of Formula XLV ##STR137## wherein Boc, R4a, and R6 are as defined above with a compound of Formula XI in the presence of methyl chloroformate and a base such as, for example, triethylamine and the like to afford a compound of Formula XLIV.
A compound of Formula III wherein R1 and R2 are bicyclic rings, which are partially or completely saturated, may be prepared from the corresponding aromatic bicyclic compound using conventional reducing conditions known in the art.
Compounds of Formula VIII, XI, XIV, XXII, XXVI, XXIX, XXXII, XXXIV, XXXVII, and XXXIX are either known or capable of being prepared by methods known in the art.
The compounds of the present invention can be prepared and administered in a wide variety of oral and parenteral dosage forms. Thus, the compounds of the present invention can be administered by injection, that is, intravenously, intramuscularly, intracutaneously, subcutaneously, intraduodenally, or intraperitoneally. Also, the compounds of the present invention can be administered by inhalation, for example, intranasally. Additionally, the compounds of the present invention can be administered transdermally. It will be obvious to those skilled in the art that the following dosage forms may comprise as the active component, either a compound of Formula I or a corresponding pharmaceutically acceptable salt of a compound of Formula I.
For preparing pharmaceutical compositions from the compounds of the present invention, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories, and dispersible granules. A solid carrier can be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material.
In powders, the carrier is a finely divided solid which is in a mixture with the finely divided active component.
In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.
The powders and tablets preferably contain from five or ten to about seventy percent of the active compound. Suitable carriers are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like. The term "preparation" is intended to include the formulation of the active compound with encapsulating material as a carrier providing a capsule in which the active component, with or without other carriers, is surrounded by a carrier, which is thus in association with it. Similarly, cachets and lozenges are included. Tablets, powders, capsules, pills, cachets, and lozenges can be used as solid dosage forms suitable for oral administration.
For preparing suppositories, a low melting wax, such as a mixture of fatty acid glycerides or cocoa butter, is first melted and the active component is dispersed homogeneously therein, as by stirring. The molten homogenous mixture is then poured into convenient sized molds, allowed to cool, and thereby to solidify.
Liquid form preparations include solutions, suspensions, and emulsions, for example, water or water propylene glycol solutions. For parenteral injection, liquid preparations can be formulated in solution in aqueous polyethylene glycol solution.
Aqueous solutions suitable for oral use can be prepared by dissolving the active component in water and adding suitable colorants, flavors, stabilizing, and thickening agents as desired.
Aqueous suspensions suitable for oral use can be made by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well-known suspending agents.
Also included are solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for oral administration. Such liquid forms include solutions, suspensions, and emulsions. These preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
The pharmaceutical preparation is preferably in unit dosage form. In such form, the preparation is subdivided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules. Also, the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form.
The quantity of active component in a unit dose preparation may be varied or adjusted from 1 mg to 1000 mg, preferably 10 mg to 100 mg according to the particular application and the potency of the active component. The composition can, if desired, also contain other compatible therapeutic agents.
In therapeutic use as anticancer agents and as agents to treat restenosis and psoriasis, and as antiviral agents, the compounds utilized in the pharmaceutical method of this invention are administered at the initial dosage of about 1 mg to about 50 mg per kilogram daily. A daily dose range of about 5 mg to about 25 mg per kilogram is preferred. The dosages, however, may be varied depending upon the requirements of the patient, the severity of the condition being treated, and the compound being employed. Determination of the proper dosage for a particular situation is within the skill of the art. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstance is reached. For convenience, the total daily dosage may be divided and administered in portions during the day if desired.
The following nonlimiting examples illustrate the inventors' preferred methods for preparing the compounds of the invention.
EXAMPLE 1
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester
Step (a) Preparation of: Bis-(1-naphthylmethyl)-acetyl-His(Trt)-OCH3
A solution of 2.0 g (4.9 mmol) of Nim -tritylhistidine methyl ester (His(Trt)-OCH3) in 20 mL of tetrahydrofuran (THF) was treated with 1.75 g (4.9 mmol) of bis-(1-naphthylmethyl)-acetyl chloride (J. Med. Chem., 35:1032 (1992)) followed by 0.7 mL (4.9 mmol) of triethylamine (Et3 N) and the mixture stirred at room temperature for 2 days. The mixture was diluted with ethyl acetate (EtOAc) and washed with H2 O, a saturated solution of sodium bicarbonate (NaHCO3), and a saturated solution of sodium chloride (NaCl). Drying over magnesium sulfate (MgSO4) and removal of the solvent under reduced pressure left 3.52 g of the crude product which was used directly in the following reaction.
Step (b) Preparation of: (S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid methyl ester
A solution of 3.52 g (4.8 mmol) of bis-(1-naphthylmethyl)-acetyl-His(Trt)-OCH3 in 250 mL of 80% acetic acid was heated at 87° C. for 15 minutes. The solvent was removed under reduced pressure and the residue taken up in EtOAc, then washed with a saturated solution of NaHCO3 and a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, eluting with chloroform/methyl alcohol (CHCl3 /MeOH) (95/5) gave 1.51 g of the product as a cream foam. The structure was confirmed by nuclear magnetic resonance spectroscopy (NMR) and mass spectroscopy; (m+H)+ =492.
EXAMPLE 2
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester
Following the procedure of Example 1, but using D-Nim -tritylhistidine methyl ester (D-HIS(Trt)-OCR3) there was obtained 1.4 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =492.
EXAMPLE 3
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid
A solution of 1.37 g (2.8 mmol) of the ester from Example 1 in 15 mL MeOH and 15 mL dioxane was treated with 6.0 mL (6.0 mmol) of 1N sodium hydroxide (NaOH) and stored at room temperature for 2 hours. The solvent was removed under reduced pressure and the residue taken up in H2 O. Acidification with 6.0 mL (6.0 mmol) of 1N hydrochloric acid (HCl) gave a gum. The solvent was decanted and nhe gum recrystallized from acetone/H2 O to give 0.59 g of a white solid, mp 193-195° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =478.
EXAMPLE 4
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid
Following the procedure of Example 3, but using the ester of Example 2, there was obtained 0.78 g of the product as a white solid, mp 187-189° C. The structure was confirmed by NMR and mass spectroscopy; (m+H+)=478.
EXAMPLE 5
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, ethyl ester
A solution of 0.5 g (1.5 mmol) of bis-(1-naphthylmethyl)-acetic acid, 0.77 g (3.0 mmol) of histidine ethyl ester dihydrochloride (His-OEt•2 HCl), and 0.22 g (1.6 mmol) of 1-hydroxybenzotriazole (HOBt) in 20 mL of THF was treated with 0.85 mL (6.0 mmol) of Et3 N followed by 0.33 g (1.6 mmol) of N,N'-dicyclo hexylcarbodiimide (DCC) and the solution allowed to stir at room temperature overnight. The mixture was filtered and the solvent removed under reduced pressure. The residue was taken up in EtOAc and washed with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave the crude product. Chromatography on silica gel, eluting with a gradient of methylene chloride (CH2 Cl2) to CH2 Cl2 /MeOH (96/4) gave 0.15 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =506.
EXAMPLE 6
(S)-3-(3-Methyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester
A suspension of 0.5 g (2.3 mmol) of π-Nim -methyl histidine methyl ester dihydrochloride (π-MeHis)-OMe•2 HCl) in 20 mL of THF was cooled in ice and 0.82 g (2.3 mmol) of bis-(1-naphthylmethyl-acetyl chloride added, followed by 1.0 mL (6.9 mmol) of Et3 N. After 0.5 hour at 0° C., the mixture was allowed to stir at room temperature overnight. The mixture was diluted with EtOAc and washed twice with H2 O, then a saturated solution of NaHCO3, then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, eluting with chloroform (CHCl3)/MeOH (97/3) followed by preparative high performance liquid chromatography (HPLC) gave a white solid when triturated with acetonitrile (CH3 CN). There was obtained 53 mg of the product as a white solid, mp 199-200° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =506.
EXAMPLE 7
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, propyl ester
Step (a) Preparation of: Bis-(1-Naphthylmethyl)-acetyl-His(Trt)-OCH3
A solution of 3.13 g (9.2 mmol) of bis-(1-naphthylmethyl)-acetic acid 4.0 g (9.2 mmol) of His(Trt)-OCH3, and 1.35 g (10.0 mmol) of HOBt in 100 mL THF was treated with 1.39 mL (10.0 mmol) of Et3 N followed by 2.09 g (10.0 mmool) of DCC. The mixture was stirred at room temperature overnight, then filtered and diluted with EtOAc. The EtOAc was washed with a saturated solution of NaHCO3, then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave 7.55 g of the crude product which was used directly in the following step.
Step (b) Preparation of: Bis-(1-Naphthylmethyl)-acetyl-His(Trt)•HCl
A solution of 7.55 g (assume 9.2 mmol) of the crude material from Step (a) above in 50 mL MeOH and 50 mL THF was treated with a solution of 1.84 g (46 mmol) of sodium hydroxide (NaOH) in 5 mL H2 O and allowed to stir at room temperature overnight. The solvent was removed under reduced pressure and the residue taken up in H2 O and acidified to the Congo red color change with dilute HCl. The aqueous mixture was extracted with EtOA and the EtOAc washed with 1N HCl, then a saturated solution of NaCl. On drying over MgSO4 and filtering, the product started to precipitate from the filtrate. There was obtained 4.0 g of the product as a white solid, mp 190-195° C. The structure was confirmed by mass spectroscopy; (m+H)+ =720.
Step (c) Preparation of: Bis-(1-Naphthylmethyl)-acetyl-His(Trt)-O-n-propyl
A solution of 0.5 g (0.7 mmol) of the acid•HCl from Step (b) above, 0.2 g (1.54 mmol) of diisopropylethylamine, and 0.44 g (0.77 mmol) of n-propanol in 20 mL CH2 Cl2 was cooled in ice and stirred for 15 minutes. The BOP reagent (benzotriazol-1-yloxy-tris(dimethylamino)-phosphonium hexafluorophosphate) (0.34 g, 0.77 mmol) was then added and the solution stirred at room temperature overnight. The mixture was then washed twice with a saturated solution of NaHCO3, then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product which was used directly in the next step.
Step (d) Preparation of: (S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, propyl ester
The material from Step (c) above was dissolved in 12 mL of 85% acetic acid and heated at 90° C. for 2 hours. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, eluting with a gradient of CH2 Cl2 to CH2 Cl2 /MeOH (96/4) gave 0.18 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =520.
EXAMPLE 8
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, isopropyl ester
Following the procedure of Example 7, but using isopropyl alcohol, there was obtained 0.2 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =520.
EXAMPLE 9
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, butyl ester
Following the procedure of Example 7, but using n-butyl alcohol, there was obtained 0.12 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =534.
EXAMPLE 10
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, benzyl ester
Following the procedure of Example 7, but using benzyl alcohol, there was obtained 0.3 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =568.
EXAMPLE 11
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclohexyl ester
Following the procedure of Example 7, but using cyclohexyl alcohol, there was obtained 0.21 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =560.
EXAMPLE 12
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclopropylmethyl ester
Following the procedure of Example 7, but using cyclopropylmethyl alcohol, there was obtained 0.15 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =532.
EXAMPLE 13
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-butyl-1H-imidazol-4-ylmethyl ester
Following the procedure of Example 7, but using 2-butyl-1H-imidazol-4-ylmethyl alcohol, there was obtained 0.08 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =614.
EXAMPLE 14
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, (±)-sec-butyl ester
Following the procedure of Example 7, but using (±)-sec-butyl alcohol, there was obtained 0.12 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =534.
EXAMPLE 15
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, allyl ester
Following the procedure of Example 7, but using allyl alcohol, there was obtained 0.15 g of a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =518.
EXAMPLE 16
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, prop-2-ynyl ester
Following the procedure of Example 7, but using prop-2-ynyl alcohol, there was obtained 0.24 g of a white solid, mp 187-189° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =516.
EXAMPLE 17
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-cyanoethyl ester
Following the procedure of Example 7, but using 2-cyanoethanol, there was obtained 0.12 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =531.
EXAMPLE 18
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-benzyloxyethyl ester
Following the procedure of Example 7, but using 2-benzyloxyethanol, there was obtained 0.12 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =612.
EXAMPLE 19
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-thiophen-2-yl-ethyl ester
Following the procedure of Example 7, but using benzotriazol-1-yloxy-tripyrrolidino phosphonium hexafluorophosphate (PyBOP) as the coupling agent and using 2-thiophen-2-yl-ethyl alcohol, there was obtained 60 mg of the product as a white solid, mp 203-206° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =588.
EXAMPLE 20
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, thiophen-3-ylmethyl ester
Following the procedure of Example 7, but using PyBOP as the coupling agent and using 2-thiophen-3-ylmethyl alcohol, there was obtained 0.12 g of the product as a white solid, mp 200-203° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =574.
EXAMPLE 21
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-diethylamino-ethyl ester
Following the procedure of Example 7, but using PyBOP as the coupling agent and using 2-diethylamino ethanol, there was obtained 0.22 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =577.
EXAMPLE 22
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-morpholin-4-yl-ethyl ester
Following the procedure of Example 7, but using PyBOP as the coupling agent and using 2-morpholin-4-yl-ethanol, there was obtained 0.12 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =591.
EXAMPLE 23
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
A solution of 0.5 g (0.7 mmol) of bis-(1-naphthylmethyl)-acetyl-His(Trt)•HCl and 0.1 g (0.77 mmol) of HOBt in 20 mL THF was treated with 0.16 g (0.77 mmol) of DCC, 0.13 g (0.7 mmol) of 2-benzyloxyethylamine•HCl, and then with 0.24 mL (1.7 mmol) of Et3 N. The mixture was stirred at room temperature overnight. The mixture was filtered and the filtrate diluted with EtOAc and washed with H2 O, a saturated solution of NaHCO3, then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave the trityl amide.
This was taken up in 12 mL of 85% acetic acid and heated at 90° C. for 2 hours. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave the crude product. Chromatography on silica gel, eluting with a gradient of hexane/EtOAc (90/10) to EtOAc/MeOH (92/8) gave 0.22 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =611.
EXAMPLE 24
(S)-N-[1-Carbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using a THF solution of ammonia (NH3) as the amine, there was obtained 0.12 g of the product obtained as an amorphous solid. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =477.
EXAMPLE 25
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-imidazol-1-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using 2-(1-imidazoyl)-ethylamine, there was obtained 0.14 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+571.
EXAMPLE 26
(S)-N-[1-(2-Ethylsulfanyl-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using 2-(ethylthio)-ethylamine•HCl, there was obtained 0.3 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =565.
EXAMPLE 27
(S)-N-{2-(1H-Imidazol-4-yl)-1-[2-(1H-imidazol-4-yl)-ethylcarbamoyl]-ethyl}-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the BOP reagent for coupling, and using histamine, there was obtained 0.1 g of the product as a white solid. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =571.
EXAMPLE 28
(S)-N-[2-(1H-Imidazol-4-yl)-1-(3-imidazol-1-yl)-propylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the BOP reagent for coupling, and using 3-(1-imidazoyl)-propylamine, there was obtained 0.17 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =585.
EXAMPLE 29
(S)-N-[1-(2-Hydroxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the BOP reagent for coupling, and using 2-hydroxy-ethylamine, there was obtained 0.08 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =521.
EXAMPLE 30
(S)-N-[2-(1H-Imidazol-4-yl)-1-isopropylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the BOP reagent for coupling, and using isopropylamine, there was obtained 0.22 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =520.
EXAMPLE 31
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-morpholin-4-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the PyBOP as the coupling reagent, and using (2-morpholin-4-yl)-ethylamine, there was obtained 0.26 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+E)+ =590.
EXAMPLE 32
(S)-N-[1-(2-Diethylamino-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the PyBOP as the coupling reagent, and using 2-diethylaminoethylamine, there was obtained 0.30 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =576.
EXAMPLE 33
(S)-N-[2-(1H-Imidazol-4-yl)-1-methylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the PyBOP as the coupling reagent, and using methylamine, there was obtained 0.22 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =491.
EXAMPLE 34
(S)-N-[1-Ethylcarbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the PyBOP as the coupling reagent, and using ethylamine, there was obtained 0.21 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =505.
EXAMPLE 35
(S)-N-{2-(1H-Imidazol-4-yl-1-[2-(4-sulfamoyl-phenyl)-ethylcarbamoyl]-ethyl}-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using the PyBOP as the coupling reagent, and using 2-(4-sulfamoylphenyl)-ethylamine, there was obtained 0.22 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =660.
EXAMPLE 36
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
Step (a) Preparation of: Bis-(1-decahydro-naphthylmethyl)-acetic acid
A solution of 1.0 g (2.9 mmol) of bis-(1-naphthylmethyl)-acetic acid in 100 mL acetic acid was reduced at 40° C., 54 pounds per square inch (psi) using 0.1 g of platinum oxide (PtO2). The solvent was removed under reduced pressure, the residue taken up in CH2 Cl2 and the solvent again removed leaving 1.0 g of the product as an oil.
Step (b) Preparation of: (S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
A solution of 0.55 g (1.5 mmol) of bis-(1-decahydronaphthylmethyl)-acetic acid, 0.73 g (1.7 mmol) of His(Trt)-OCH3 •HCl, and 0.23 g (1.7 mmol) of HOBt in 30 mL THF was treated with 0.35 g (1.7 mmol) of DCC followed by 0.3 mL (1.7 mmol) of Et3 N and the mixture stirred at room temperature overnight. The mixture was filtered and the solvent removed under reduced pressure. The residue was taken up in EtOAc and washed with H2 O, a saturated solution of NaHCO3, and a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave the crude trityl derivative.
This was taken up in 12 mL of 85% acetic acid and heated at 90° for 2 hours. The solvent was removed under reduced pressure and the residue taken up in EtOAc, washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave the crude product. Chromatography on silica gel, eluting with a gradient of EtOAc/hexane (10/90) to EtOAc/MeOH (96/4) gave 0.35 g of the product as a yellow foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =512.
EXAMPLE 37
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, methyl ester
Step (a) Preparation of: Dimethyl bis-(5,6,7,8-tetrahydro-1-naphthylmethyl)-malonate
To a suspension of 0.77 g (0.02 mol) of sodium hydride (NaH)•oil (60%) in 30 mL THF, cooled to 0° C. was added dropwise 1.06 g (8.0 mmol) of dimethyl malonate. After stirring for 15 minutes, the mixture was treated with 3.6 g (16 mmol) of 1-bromomethyl-5,6,7,8-tetrahydro-naphthalene (Chemical Abstracts, 75:76445 (1971)). After stirring at room temperature overnight, the mixture was diluted with EtOAc and washed with 1N HCl, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave 3.1 g of an oil which solidified on standing. The structure was confirmed by NMR spectroscopy.
Step (b) Preparation of Bis-(5,6,7,8-tetrahydro-1-naphthylmethyl)-acetic acid
A solution of 3.1 g (7.4 mmol) of dimethyl bis-(5,6,7,8-tetrahydro-1-naphthylmethyl)-malonate in 20 mL n-butanol was treated with a solution of 1.24 g (22 mmol) of potassium hydroxide (KOH) in 20 mL H2 O and the solution heated at reflux overnight. The solvent was removed under reduced pressure and the residue taken up in H2 O and acidified with dilute HCl to the Congo red color change. The mixture was extracted with EtOAc and the EtOAc washed with 1N HCl, and then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure gave 2.03 g of the product. A small amount recrystallized from EtOAc/hexane had mp 144-146° C. The structure was confirmed by NMR spectroscopy.
Step (c) (S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydronaphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, methyl ester
Following the procedure of Example 36, Step (b), there was obtained 0.55 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =500.
EXAMPLE 38
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionamide
Following the procedure of Example 36, Step (b), but using PyBOP as the coupling agent, and using 2-benzyloxy-ethylamine, there was obtained 0.25 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =619.
EXAMPLE 39
(S)-2-(3-Benzo[b]thiophen-3-yl-2-benzo[b]thiophen-3-ylmethyl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
Step (a) Preparation of: Dimethyl bis-(benzo[b]thiophen-3-ylmethyl)-malonate
A suspension of 0.34 g (8.5 mmol) of NaH•oil (60%) in 30 mL THF was treated with 0.45 g (3.4 mmol) of dimethyl malonate and stirred at room temperature for 15 minutes. The mixture was then treated with 1.55 g (6.8 mmol) of 3-bromomethyl-benzo[b]thiophene and the mixture then heated at reflux overnight. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed twice with 1N HCl, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left 1.33 g of the product as an oil. The structure was confirmed by NMR spectroscopy.
Step (b) Preparation of: Bis-(benzo[b]thiophen-3-ylmethyl)-acetic acid
A solution of 1.3 g (3.3 mmol) of the material from Step (a) above in 10 mL n-butanol was treated with a solution of 0.52 g (9.3 mmol) of KOH in 10 mL H2 O and heated at reflux overnight. The solvent was removed under reduced pressure and the residue taken up in H2 O and acidified with dilute HCl. The mixture was extracted with EtOAc and washed with 1N HCl, and then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product which was recrystallized from EtOAc/hexane to give 0.58 g of the product as a white solid. The structure was confirmed by NMR spectroscopy.
Step (c) Preparation of: (S)-2-(3-Benzo[b]-thiophen-3-yl-2-benzo[b]thiophen-3-ylmethyl-propionylamino-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
Following the procedure of Example 36, Step (b), but using the BOP reagent as the coupling agent, and using bis-(benzo[b]thiophen-3-ylmethyl)-acetic acid, there was obtained 0.2 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =504.
EXAMPLE 40
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-thiopropionic acid, S-(2-acetylamino-ethyl)ester
Following the procedure of Example 23, but using 2-acetylthioethylamine•HCl, there was obtained 0.82 g of the rearranged thio-ester as the product. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =579.
EXAMPLE 41
(S)-N-[1-(2-Cyano-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using 2-cyanoethylamine, there was obtained 0.2 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =530.
EXAMPLE 42
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-hydroxy-ethyl ester, trifluoroacetate salt
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using ethylene glycol, there was obtained 0.13 g of the crude product. Purification by preparative HPLC gave 80 mg of the pure product as the trifluoroacetate salt. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =522.
EXAMPLE 43
(S)-N-[1-Dimethylcarbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using dimethylamine, there was obtained 75 mg of the product. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =505.
EXAMPLE 44
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-ynyl ester, trifluoroacetate salt
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using 3-butyn-1-ol, there was obtained 0.32 g of the crude product. Purification by preparative HPLC gave 30 mg of the pure product as the trifluoroacetate salt. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =530.
EXAMPLE 45
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, 2-cyano-ethyl ester
Following the procedure of Example 23, Step (b), but using PyBOP as the coupling agent, and using 2-cyanoethanol, there was obtained 70 mg of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =552.
EXAMPLE 46
(S)-N-[2-(1H-Imidazol-4-yl)-1-propylcarbamoyl-ethyl]-3-naphthalen-1-yl)-2-naphthalen-1-ylmethyl)-propionamide
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using n-propylamine, there was obtained 50 mg of the product. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =519.
EXAMPLE 47
(S)-3-[1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-imidazol-1-yl-ethyl ester
Following the procedure of Example 23, but using BOP reagent as the coupling agent, and using 2-(1-imidazol)-ethanol, there was obtained 70 mg of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =572.
EXAMPLE 48
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-enyl ester
Following the procedure from Example 23, but using the BOP reagent as the coupling agent, and using 3-buten-1-ol, there was obtained 160 mg of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =532.
EXAMPLE 49
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, 2-cyano-ethyl ester
Following the procedure of Example 37, Step (c), but using the BOP reagent as the coupling agent, and using 2-cyanoethanol, there was obtained 110 mg of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =539.
EXAMPLE 50
(S)-N-[2-(1H-Imidazol-4-yl)-1-phenethylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl)-propionamide
Following the procedure of Example 23, but using PyBOP as the coupling agent, and using phenethylamine, there was obtained 61 mg of the product. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =581.
EXAMPLE 51
(S)-3-(1H-Imidazol-4-yl-2-[methyl-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionyl)-amino]-propionic acid, methyl ester, trifluoroacetate
A suspension of 0.56 g (2.2 mmol) of N-MeHIS-OMe•2 HCl in 15 mL dimethylacetamide (DMA) was treated with 0.8 mL (6.6 mmol) of N-methylpiperidine followed by a solution of 0.79 g (2.2 mmol) of bis-(1-naphthylmethyl)-acetyl chloride in 5 mL CH2 Cl2, and the mixture was stirred at room temperature for 2 days. The mixture was diluted with EtOAc and washed three times with H2 O, a saturated solution of NaHCO3, and then a saturated solution of NaCl. Drying over MgSO4, and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, eluting with CH2 Cl2 /MeOH (99/1) gave 0.4 g of the partly purified product. Preparative HPLC gave 42 mg of the pure product as the trifluoroacetate salt. The structure was confirmed by mass spectroscopy; (m+H)+ =506.
EXAMPLE 52
(S)-N-[1-Hydroxymethyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
A solution of 0.5 g (1.0 mmol) of the product from Example 1 in 15 mL of THF was cooled in ice and treated with 88 mg (4.0 mmol) of lithium borohydride. After 15 minutes at 0° C., the mixture was allowed to warm to room temperature over 1 hour. The mixture was acidified with 5 mL of 2N HCl, then made basic with a saturated solution of NaHCO3, and extracted with EtOAc. The EtOAc solution was washed with a saturated solution of NaCl, dried over MgSO4, and the solvent removed under reduced pressure giving the crude product. Chromatography on silica gel, eluting with CHCl3 /MeOH (95/5) gave 310 mg of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =464.
EXAMPLE 53
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, phenethyl ester
This compound was prepared following the procedure of Example 45. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =582.
EXAMPLE 54
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionyiamino)-propionic acid, 3-cyano-propyl ester
This compound was prepared following the procedure of Example 45. The structure was confirmed by NNR and mass spectroscopy; (m+H)+ =545.
EXAMPLE 55
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid 3-methyl-but-2-enyl ester
This compound was prepared following the procedure of Example 47. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =546.
EXAMPLE 56
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionic acid methyl ester
Step (a) Preparation of Bis-(1-naphthylmethyl)-acetic acid, O,N-dimethylamide
To a solution of 5.0 g (13.9 mmol) of bis-(1-naphthylmethyl)acetyl chloride in 30 mL CH2 Cl2 cooled in ice was added a solution of 1.36 g (13.9 mmol) of O,N-dimethylhydroxylamine•HCl and 1.7 mL (13.9 mmol) of N-methylpiperidine in 25 mL CH2 Cl2. This was followed with an additional 1.7 mL (13.9 mmol) of N-methylpiperidine. The cooling was removed and the mixture allowed to stir at room temperature overnight. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed with 1N HCl, H2 O, a saturated solution of NaHCO3, and a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography over silica gel, and eluting with CHCl3 gave 3.48 g of the product as an oil which crystallized, mp 101-103° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =384.
Step (b) Preparation of Bis-(1-naphthylmethyl)-acetaldehyde
A solution of 3.48 g (9.1 mmol) of bis-(1-naphthylmethyl)-acetic acid, O,N-dimethylamide in 50 mL of tetrahydrofuran was cooled in ice and treated rapidly in portions with 0.45 g (11.8 mmol) of lithium aluminum hydride. After stirring at 0° C. for 45 minutes, the mixture was decomposed with a solution of KHSO4 in H2 O. The mixture was diluted with EtOAc, the pH adjusted to 4, an the layers separated. The EtOAc layer was washed with 1N HCl, H2 O, a saturated solution of NaHCO3, and a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left 2.35 g of the product as an oil which crystallized. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =324.
Step (c) Preparation of (S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionic acid, methyl ester
A solution of 3.41 g (10.5 mmol) of bis-(1-naphthylmethyl)-acetaldehyde in 60 mL of tetrahydrofuran was treated with 4.32 g (10.5 mmol) of HIS(Trt)-OMe and 14 g of activated 3A molecular sieves and stirred at room temperature overnight. A pinch of bromocresol green was added, and the color adjusted to a light green with 1N HCl in dioxane, and the mixture then treated with 1.2 g (18 mmol) of sodium cyanoborohydride. After stirring at room temperature overnight, the mixture was filtered and the sieves washed with tetrahydrofuran. The organic phase was diluted with EtOAc and washed with a saturated solution of NaHCO3, H2 O, and a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left 7.27 g of the crude product as a cream foam. This was the trityl compound complexed with BH2 CN.
A solution of 1.31 g (1.7 mmol) of this complex in 80 mL of 88% formic acid was heated at 89° C. for 0.5 hour. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, and eluting with CHCl3 /MeOH (95/5) gave 480 mg of the pure product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =478.
EXAMPLE 57
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methoxycarbonylmethyl ester
This compound was prepared following the procedure of Example 47. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =550.
EXAMPLE 58
(S )-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyanomethyl ester
This compound was prepared following the procedure of Example 47. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =517.
EXAMPLE 59
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-(2-hydroxy-ethyldisulfanyl)-ethyl ester
This compound was prepared following the procedure of Example 47. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =614.
EXAMPLE 60
(S)-3-(3-Methoxymethyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester
A solution of 0.8 g (1.1 mmol) of bis-(1-naphthylmethyl)-acetyl-HIS(Trt)-OCH3 in 15 mL CH2 Cl2 was treated with 0.2 mL (2.4 mmol) of chloromethylmethyl ether and allowed to stir at room temperature for 3 days. The mixture was diluted with 80 mL of 80% acetic acid and heated at 89° C. for 0.5 hour. The solvent was removed under reduced pressure and the residue taken up in EtOAc, and washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, and eluting with CHCl3 /MeOH (97/3) gave 0.17 g of the pure product as a solid, mp 182-185° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =536.
EXAMPLE 61
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, (±)-1-cyano-ethyl ester
This compound was prepared following the procedure of Example 47. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =531.
EXAMPLE 62
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propan-1-ol
A solution of 1.0 g (1.3 mmol) of the complex from Example 56, Step (c), in 20 mL of tetrahydrofuran was cooled in ice and treated with 144 mg (6.6 mmol) of lithium borohydride. The cooling was removed and the mixture allowed to stir at room temperature for 1.75 hours. The mixture was decomposed with 2N HCl, then made basic with a saturated solution of NaHCO3, and extracted with EtOAc. The EtOAc was washed with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left 0.95 g of the reduced complex.
This was taken up in 60 mL of 88% formic acid and heated at 89° C. for 0.5 hour. The solvent was removed under reduced pressure and the residue taken up in 30 mL MeOH and treated with a solution of 2 g NaOH in 10 mL H2 O. After stirring at room temperature overnight, the solution was treated with 22 mL of 2N HCl and stripped to dryness under reduced pressure. The residue was taken up in EtOAc and washed with a saturated solution of NaHCO3, then a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, and eluting with CHCl3 /MeOH (90/10) gave 0.15 g of the pure product as a cream foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =450.
EXAMPLE 63
(S)-3-(1H-Imidazol-4-yl)-N-methyl-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionamide
A solution of 1.4 g (1.8 mmol) of the complex from Example 56, Step (c), in 15 mL MeOH and 15 mL of tetrahydrofuran was cooled in ice and saturated with methylamine gas and allowed to stir at room temperature for 2 days. The solvent was removed under reduced pressure leaving a brown foam.
This was taken up in 80 mL of 88% formic acid and heated at 89° C. for 0.5 hour. The solvent was removed under reduced pressure and the residue taken up in EtOAc and washed twice with a saturated solution of NaHCO3, then with a saturated solution of NaCl. Drying over MgSO4 and removal of the solvent under reduced pressure left the crude product. Chromatography on silica gel, and eluting with CHCl3 /MeOH (95/5) gave 0.59 g of the product as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =477.
EXAMPLE 64
(S)-N-[1-Methylcarbamoyl-2-(3-methyl-3H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide
A solution of 611 mg (1.2 mmol) of the product from Example 6 in 20 mL MeOH and 10 mL tetrahydrofuran was cooled in ice and saturated with gaseous methylamine. The cooling was removed and the solution allowed to stir at room temperature overnight. The solvent was removed under reduced pressure and the residue triturated with acetonitrile to give 529 mg of the pure product, mp 250-252° C. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =505.
EXAMPLE 65
(S)-2-(2-Benzyl-3-naphthalen-1-yl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester
Following the procedure of Example 7, Steps (a)-(d), but using benzyl-(α-naphthylmethyl)-acetic acid (Ann., 468:300 (1929)), the product is obtained after preparative HPLC as a white foam. The structure was confirmed by NMR and mass spectroscopy; (m+H)+ =442.

Claims (14)

We claim:
1. A compound of Formula I ##STR138## wherein R is hydrogen or alkyl; R1 and R2 are the same or different and are selected from the group consisting of: ##STR139## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and R7 is 1 to 3 substituents selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkoxy,
thioalkoxy,
hydroxy,
mercapto,
halogen,
nitro, ##STR140## wherein R8 and R9 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl, or R8 and R9 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is hydrogen or alkyl, and
n is zero or an integer of one to four, ##STR141## wherein R9a is hydrogen or alkyl, and R8, R9, and n are as defined above, and ##STR142## wherein R11 is selected from the group consisting of: hydrogen,
alkyl, and
aryl, ##STR143## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
NR12 wherein R12 is hydrogen, alkyl or ##STR144## wherein R8, R9, and n are as defined above, or R12 is absent,
O,
S,
SO, and
SO2, and
Z is at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH2 unit and R12 is absent, and R7 is as defined above, ##STR145## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z and R7 are as defined above, and R12 is present ##STR146## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z and R7 are as defined above, and R12 is present, ##STR147## wherein the monocyclic ring is either aromatic, or partially or completely saturated, and R7 is as defined above with the proviso that R1 and R2 are not both a monocyclic ring, and ##STR148## wherein the monocyclic ring is either aromatic, or partially or completely saturated, and R7 and Z are as defined above with the proviso that R1 and R2 are not both a monocyclic ring;
R3 is hydrogen or alkyl;
R4 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
benzyl,
alkyl chain wherein the alkyl chain is interrupted by a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above, ##STR149## wherein p is an integer of one to four, and R13 is alkyl or benzyl, and ##STR150## wherein p and R13 are as defined above; X is ##STR151## Y is ##STR152## R5 is selected from the group consisting of: --OR14 wherein R14 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
hydroxyalkyl,
mercaptoalkyl,
cyanoalkyl,
nitroalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR153## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
HO2 C-alkyl,
alkyl-O2 C-alkyl, and ##STR154## wherein R15 and R16 are as defined above, --S--R14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, ##STR155## wherein R17 and R18 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
cyanoalkyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
mercaptoalkyl,
nitroalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR156## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
or R17 and R18 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
--NH--OR10 wherein R10 is as defined above,
alkyl,
alkenyl, and
arylalkyl; and
R6 is hydrogen,
--SR where R is as defined above,
--OR where R is as defined above, or ##STR157## wherein R and Ra are the same or different and are as defined above for R;
and when X is --CH2 -- and R17 is hydrogen or alkyl then R18 may be ##STR158## wherein R is as defined above, or ##STR159## wherein R is as defined above; and when X is ##STR160## must be ##STR161## and excluding the compound wherein
R is hydrogen,
R1 and R2 are each ##STR162## R3 is hydrogen, R4 is hydrogen,
X is ##STR163## Y is ##STR164## R5 is OR14 wherein R14 is hydrogen, and R6 is hydrogen;
or corresponding isomers thereof,
or a pharmaceutically acceptable salt thereof.
2. A compound according to claim 1 wherein
R is hydrogen;
R7 is selected from the group consisting of:
hydrogen,
methoxy,
thiomethoxy,
hydroxy,
halogen, and ##STR165## wherein R8 and R9 are the same or different and are selected from the group consisting of:
hydrogen, and
alkyl;
R3 is hydrogen or methyl;
R4 is selected from the group consisting of:
hydrogen,
methyl,
ethyl, and
--CH2 --O--CH3 ;
X is ##STR166## Y is ##STR167## R5 is selected from the group consisting of: --O--R14 wherein R14 is selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
hydroxyallyl,
mercaptoalkyl,
cyanoalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR168## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: O, and NR10 wherein R10 is hydrogen or methyl, and
alkyl-O2 C-alkyl,
--S--R14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, and ##STR169## wherein R19 is hydrogen,
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
cyanoalkyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
mercaptoalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR170## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above, and
R20 is hydrogen or methyl; and
R6 is hydrogen.
3. A compound according to claim 2 wherein R1 and R2 are the same or different and are selected from the group consisting of: ##STR171## R3 is hydrogen or methyl; R4 is hydrogen, methyl, or --CH2 OCH3 ;
X is --CH2 -- or ##STR172## Y is --CH2 --, or ##STR173## and R5 is selected from the group consisting of:
--O--R14 wherein R14 is selected from the group consisting of: ##STR174## --SR14 wherein R14 is as defined above with the proviso that R14 is not hydrogen, and ##STR175## wherein R17 is selected from the group consisting of: ##STR176## R18 is hydrogen or methyl, and --NH--OR10 wherein R10 is hydrogen or methyl.
4. A compound according to claim 3 selected from the group consisting of:
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1R-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid;
(R)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, ethyl ester;
(S)-3-(3-Methyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, propyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, isopropyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, butyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, benzyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclohexyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyclopropylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-butyl-1H-imidazol-4-ylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, (±)-sec-butyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, allyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, prop-2-ynyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-cyano-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-benzyloxy-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-thiophen-2-yl-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, thiophen-3-ylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-diethylamino ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-morpholin-4-yl-ethyl ester;
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(Carbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-imidazol-1-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-Ethylsulfanyl-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-{2-(1H-Imidazol-4-yl)-1-[2-(1H-imidazol-4-yl)-ethylcarbamoyl]-ethyl}-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(3-imidazol-1-yl)-propylcarbamoyl]-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(2-Hydroxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-isopropylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-(2-morpholin-4-yl-ethylcarbamoyl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-(2-Diethylamino-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[2-(1H-Imidazol-4-yl)-1-methylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-[1-Ethylcarbamoyl-2-(1H-Imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-N-{2-(1H-Imidazol-4-yl)-1-[2-(4-sulfamoyl-phenyl)-ethylcarbamoyl]-ethyl}-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, methyl ester;
(S)-N-[1-(2-Benzyloxy-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionamide;
(S)-2-(3-Benzo[b]thiophen-3-yl-2-benzo[b]thiophen-3-ylmethyl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-thiopropionic acid, S-(2-acetylamino-ethyl) ester;
(S)-N-[1-(2-Cyano-ethylcarbamoyl)-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-hydroxy-ethyl ester;
(S)-N-[1-Dimethylcarbamoyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-ynyl ester;
(S)-2-[3-(Decahydro-naphthalen-1-yl)-2-(decahydro-naphthalen-1-ylmethyl)-propionylamino]-3-(1H-imidazol-4-yl)-propionic acid, 2-cyano-ethyl ester;
(S)-N-[2-(1H-Imidazol-4-yl)-1-propylcarbamoyl-ethyl]3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-imidazol-1-yl-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, but-3-enyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-[3-(5,6,7,8-tetrahydro-naphthalen-1-yl)-2-(5,6,7,8-tetrahydro-naphthalen-1-ylmethyl)-propionylamino]-propionic acid, 2-cyano-ethyl ester;
(S)-N-[2-(1H-imidazol-4-yl)-1-phenethylcarbamoyl-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-(1H-Imidazol-4-yl)-2-[methyl-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propiony)-amino]-propionic acid, methyl ester;
(S)-N-[1-Hydroxymethyl-2-(1H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide;
(S)-3-[1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, phenethyl ester;
(S)-3-[1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 3-cyano-propyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 3-methyl-but-2-enyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methoxycarbonylmethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, cyanomethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 2-(2-hydroxy-ethyldisulfanyl)-ethyl ester;
(S)-3-(3-Methoxymethyl-3H-imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, methyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionylamino)-propionic acid, 1-cyano-ethyl ester;
(S)-3-(1H-Imidazol-4-yl)-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propan-1-ol;
(S)-3-(1H-Imidazol-4-yl)-N-methyl-2-(3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propylamino)-propionamide;
(S)-N-[1-Methylcarbamoyl-2-(3-methyl-3H-imidazol-4-yl)-ethyl]-3-naphthalen-1-yl-2-naphthalen-1-ylmethyl-propionamide; and
(S)-2-(2-Benzyl-3-naphthalen-1-yl-propionylamino)-3-(1H-imidazol-4-yl)-propionic acid, methyl ester.
5. A method of treating tissue proliferative diseases comprising administering to a host suffering therefrom a therapeutically effective amount of a compound according to claim 1 in unit dosage form.
6. A method of treating cancer comprising administering to a host suffering therefrom a therapeutically effective amount of a compound according to claim 1 in unit dosage form.
7. A method of treating restenosis comprising administering to a host suffering therefrom a therapeutically effective amount of a compound according to claim 1 in unit dosage form.
8. A method of treating psoriasis comprising administering to a host suffering therefrom a therapeutically effective amount of a compound according to claim 1 in unit dosage form.
9. A method of treating viral infections comprising administering to a host suffering therefrom a therapeutically effective amount of a compound according to claim 1 in unit dosage form.
10. A pharmaceutical composition comprising a compound according to claim 1 in admixture with a pharmaceutically acceptable excipient, diluent, or carrier.
11. A pharmaceutical composition adapted for administration as an antiproliferative agent comprising a therapeutically effective amount of a compound according to claim 1 in admixture with a pharmaceutically acceptable excipient, diluent, or carrier.
12. A pharmaceutical composition adapted for administration as an anticancer agent, or restenosis inhibiting agent or antipsoriasis agent or antiviral agent comprising a therapeutically effective amount of a compound according to claim 1 in admixture with a pharmaceutically acceptable excipient, diluent, or carrier.
13. A method of treating tissue proliferative diseases, cancer, restenosis, psoriasis and viral infections, comprising administering to a host suffering therefrom a therapeutically effective amount of a compound of formula ##STR177## and corresponding isomers thereof; or a pharmaceutically acceptable salt thereof in unit dosage form.
14. A method for preparing a compound having the Formula Ia ##STR178## wherein R is hydrogen or alkyl; R1 and R2 are the same or different and are selected from the group consisting of: ##STR179## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and R7 are 1 to 3 substituents selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkoxy,
thioalkoxy,
hydroxy,
mercapto,
halogen,
nitro, ##STR180## wherein R8 and R9 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl, or R8 and R9 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is hydrogen or alkyl, and
n is zero or an integer of one to four, ##STR181## wherein R9a is hydrogen or alkyl, and R8, R9, and n are as defined above, and ##STR182## wherein R11 is selected from the group consisting of: hydrogen,
alkyl, and
aryl, ##STR183## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z is selected from the group consisting of:
NR12 wherein R12 is hydrogen, alkyl or ##STR184## wherein R8, R9, and n are as defined above, or R12 is absent,
O,
S,
SO, and
SO2, and
Z is at other positions in the bicyclic ring system provided that when the bicyclic ring is aromatic, Z is not at the point of attachment of the CH2 unit and R12 is absent, and R7 is as defined above, ##STR185## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z and R7 are as defined above and R12 may be present, ##STR186## wherein the bicyclic ring is either aromatic, or partially or completely saturated, and Z and R7 are as defined above and R12 is present, ##STR187## wherein the monocyclic ring is either aromatic, or partially or completely saturated, and R7 is as defined above with the proviso that R1 and R2 are not both a monocyclic ring, and ##STR188## wherein the monocyclic ring is either aromatic, or partially or completely saturated, and R7 and Z are as defined above with the proviso that R1 and R2 are not both a monocyclic ring;
R3 is hydrogen or alkyl;
Xa is ##STR189## Y is ##STR190## R5a is selected from the group consisting of: --OR14a wherein R14a is selected from the group consisting of:
alkyl,
alkenyl,
alkynyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
hydroxyalkyl,
mercaptoalIyl,
cyanoalkyl,
nitroalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
thioalkoxyalkyl,
acetamidoalkyl,
HOCH2 CH2 --S--S--CH2 CH2 --, ##STR191## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
allyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
HO2 C-alkyl,
alkyl-O2 C-alkyl, and ##STR192## wherein R15 and R16 are as defined above, --S--R14a wherein R14a is as defined above; ##STR193## wherein R17 and R18 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl,
alkenyl,
alkynyl,
cyanoallyl,
hydroxyalkyl,
alkoxyalkyl,
arylalkyl,
heteroarylalkyl,
benzyloxyalkyl,
cycloalkyl,
cycloalkylalkyl,
haloalkyl,
mercaptoalkyl,
nitroalkyl,
thioalkoxyalkyl,
acetamidoalkyl, ##STR194## wherein R15 and R16 are the same or different and are selected from the group consisting of:
hydrogen,
alkyl or R15 and R16 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
or R17 and R18 are taken together with N to form a 5- or 6-membered ring which does or does not contain a heteroatom selected from the group consisting of: S, O, and N--R10 wherein R10 is as defined above,
--NH--OR10 wherein R10 is as defined above,
alkyl,
alkenyl, and
arylalkyl; and
R6 is hydrogen,
--SR where R is as defined above,
--OR where R is as defined above, or ##STR195## wherein R and Ra may be the same or different and are as defined above for R; or corresponding isomers thereof; or a pharmaceutically acceptable salt thereof comprising reacting a compound of Formula II ##STR196## wherein Trt is (C6 H5)3 --C-- and R, R1, R2, R3, Xa, Y, R5a, and R6 are as defined above with an a to afford a compound of Formula Ia.H
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