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WO1991001366A1 - Procede de stabilisation de bacteries par centrifugation en une pate non aqueuse - Google Patents

Procede de stabilisation de bacteries par centrifugation en une pate non aqueuse Download PDF

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Publication number
WO1991001366A1
WO1991001366A1 PCT/GB1990/001093 GB9001093W WO9101366A1 WO 1991001366 A1 WO1991001366 A1 WO 1991001366A1 GB 9001093 W GB9001093 W GB 9001093W WO 9101366 A1 WO9101366 A1 WO 9101366A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
bacterial
aqueous
paste
liquid
Prior art date
Application number
PCT/GB1990/001093
Other languages
English (en)
Inventor
Frank L. Horsfall, Iii
Original Assignee
Biosphere Corporation S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosphere Corporation S.A. filed Critical Biosphere Corporation S.A.
Publication of WO1991001366A1 publication Critical patent/WO1991001366A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

Definitions

  • bacreria Once bacreria have completed their growth cycle in a particular medium or environment, they will either start dying or form a spore which can withstand long periods of starvation, dehydration, or extremes of temperatures. Since most types of bacteria cannot form environmentally resistant spores, their viability can be enhanced only by finding conditions or chemicals which prevent or at least decrease their rate of dying. Examples of chemicals which can sustain non-spore-forming bacteria from decomposition with time have been patented previously by Horsfall et al in 1976 using aqueous sulfide levels and by Wong et al in 1987 using aqueous azide levels. These inhibitory chemicals caused nonsporulating bacteria to remain viable over long periods of time without bacterial lysis, cell death from disintegration of the cell wall.
  • the process of sporulation, as well as bacterial growth during the last stages of food or nutrient consumption can involve the production of extracellular enzymes from the bacterial cytoplasm or from excretion.
  • the purpose of these enzymes can be to create food for the bacteria from particulate organic matter. Since these enzymes can attack the components of the bacterial cell wall, their presence is deleterious to any but rapidly growing and reproducing bacteria. It would be a distinct advantage to be able to store bacteria grown to high levels and in the presence of these exoenzymes , free of their deleterious effects.
  • removing totally the aqueous environment from the bacteria through freeze or air drying can accomplish this condition.
  • This technique also concentrates all the salts as well as the enzymes and bacterial components of the system being dried. This may be one of the reasons for the high levels of bacterial death experienced during these drying techniques .
  • the present invention seeks to provide an improvement.
  • a method of stabilising bacteria present in an aqueous growth medium consisting in separating the bacteria from the growth medium by centrifuging to make a non-aqueous bacterial paste and storing the paste in a protective environment.
  • the protective environment may comprise an impermeable sheath made of Visking, Saran or like material.
  • the protective environment may be liquid, preferably non-aqueous, but possibly aqueous and possibly water. Re-suspension of the paste in a liquid allows the bacteria to be very much more concentrated than in their original environment.
  • Preferred non-aqueous liquids are glycerol, glycol or the like .
  • the paste either itself or when re-suspended in liquid, may be encapsulated in a non-permeable but dissolving or disintegrating degradable membrane such as gelatin.
  • the preparation of such a paste is critical to retain bacterial viability and longevity.
  • the centrifuged paste must be kept cool , free from evaporative losses and free from oxygen. To do this, it is necessary to use a continuous flow centrifuge such as a Sharpies and an insert within the centrifuge made of a material like Visking or Saran so the bacteria are deposited inside a cylindrical sheath as one would pack a sausage.
  • the process described would produce a bacterial paste contained inside an impervious casing which would not age, since the aqueous environment, the enzymes, the nutrients, and the salts would be removed during the centrifugation process and remain with the water phase.
  • Such bacterial sausage preparations can be used for many purposes especially pertaining to but not limited to the wastewater treatment industry such as:
  • stored cultures would provide a means of immediate on site relief from such eventualities by having immediately usable bacteria with which to grow a dense seed culture to be introduced into the plant.
  • Such a sausage could function to restart a treatment plant severally affected by conditions or toxic problems or as a supplemental seed to optimise and enhance operations of the plant
  • the sausage provides a means to be able to transport huge numbers of viable bacteria in a concentrated and immediately available form from one site to another, even across vast
  • glycerol - glycerin ethylene glycol 1,2-propylene glycol 1 , 3-propylene glycol.
  • Introducing the bacteria from a paste into such media would result in a stable suspension because, even though these liquid media are biodegradable, there would be neither water nor nutrients available for bacterial growth in their preparation.
  • the growth of bacteria or, for that matter, any living form, requires four basic ingredients; carbon, nitrogen, phosphorus, and water.
  • anhydrous liquid like glycerol, for example, will not provide the needed conditions for their growth, since neither nitrogen, phosphorus, nor water is part of glycerol, ethylene glycol, or the various isomers of propylene glycol.
  • the bacterial suspensions which are formed from the bacterial paste added to the non-aqueous , anhydrous liquids will be deficient in the nutrients necessary for bacterial growth and replication until they are added to a growth sustaining system. The stability of the system is provided by the growth limiting and anhydrous condition of the liquid suspension itself.
  • a method for incorporating bacteria suspended in a substance more dense than fresh or sea water into a form to provide a system for slow delivery is hereby described.
  • Encapsulating the liquid suspension produced using a material like gelatin which would allow a slow release of the bacterial suspension over time after the encapsulated liquid had sunk to the bottom of the water body would provide just the necessary system to allow for isolated use of the liquid suspension.
  • any solid or semi-solid coating that would allow a period of time to elapse before allowing the release of the liquid suspension contained within it would suffice.
  • the basic requirement for the coating would have to be limited solubility in water and insolubility in the liquid used to create the suspension of bacteria it coated. Since such systems are readily available commercially and in use in the manufacture of pills like vitamins they will not be discussed herein. In the case of glycerol, for example, a capsule similar to that sold as Vitamin E would be entirely sufficient.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de stabilisation de bactéries se trouvant dans un milieu de croissance aqueux, ledit procédé consistant à séparer les bactéries du milieu de croissance par centrifugation afin de produire une pâte bactérienne non aqueuse, et à stocker ladite pâte dans un environnement de protection. On peut centrifuger ladite pâte afin d'obtenir une enveloppe imperméable permettant de fabriquer une saucisse ou on peut la remettre en susension dans un liquide et éventuellement l'encapsuler dans de la gélatine.
PCT/GB1990/001093 1989-07-14 1990-07-16 Procede de stabilisation de bacteries par centrifugation en une pate non aqueuse WO1991001366A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898916152A GB8916152D0 (en) 1989-07-14 1989-07-14 A method for stabilising bacteria
GB8916152.5 1989-07-14

Publications (1)

Publication Number Publication Date
WO1991001366A1 true WO1991001366A1 (fr) 1991-02-07

Family

ID=10660051

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1990/001093 WO1991001366A1 (fr) 1989-07-14 1990-07-16 Procede de stabilisation de bacteries par centrifugation en une pate non aqueuse

Country Status (6)

Country Link
EP (1) EP0437564A1 (fr)
JP (1) JPH04505262A (fr)
AU (2) AU6033990A (fr)
CA (1) CA2036391A1 (fr)
GB (1) GB8916152D0 (fr)
WO (1) WO1991001366A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0559062A1 (fr) * 1992-03-06 1993-09-08 AGRA SOCIETA' DEL SEME S.r.l. Composition pour l'apprétage des semences, semences apprétées par cette composition et procédé par l'apprétage correspondant
WO2001005949A1 (fr) * 1999-07-15 2001-01-25 Unilever N.V. Procede de separation de membrane destine a purifier, a concentrer et a densifier les solutions d'enzymes avec des polyols
US6420333B1 (en) 2001-08-28 2002-07-16 Unilever Home & Personal Care Usa Division Of Conopco, Inc. Manufacture of capsules for incorporation into detergent and personal care compositions
US6730651B2 (en) 2001-08-28 2004-05-04 Unilever Home & Personal Care Usa Division Of Conopco. Inc. Concentrated stock of capsules for detergent or personal care compositions
CN101560491B (zh) * 2008-04-15 2012-01-04 中国科学院上海生命科学研究院 一种发光细菌及其在食品或水样总体生物毒性检测中的应用
WO2020049188A1 (fr) * 2018-09-07 2020-03-12 Nolivade Composition et procédé de conservation
KR20210031956A (ko) * 2018-07-20 2021-03-23 지멘스 에너지 글로벌 게엠베하 운트 코. 카게 나선형으로 감긴 도체 스트랜드를 갖는 장치 및 이러한 장치를 제조하기 위한 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE513511C (de) * 1927-12-20 1930-11-28 Olga Klara Sauer Geb Buchholz Verfahren zur Herstellung haltbarer keimfaehiger Hefe- und Bakterienpraeparate
FR1272116A (fr) * 1960-08-09 1961-09-22 Procédé et récipient de conservation des levures
EP0202409A2 (fr) * 1985-03-25 1986-11-26 Miles Inc. Procédé de préparation de micro-organismes secs, viables et stables pour l'alimentation et l'agriculture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE513511C (de) * 1927-12-20 1930-11-28 Olga Klara Sauer Geb Buchholz Verfahren zur Herstellung haltbarer keimfaehiger Hefe- und Bakterienpraeparate
FR1272116A (fr) * 1960-08-09 1961-09-22 Procédé et récipient de conservation des levures
EP0202409A2 (fr) * 1985-03-25 1986-11-26 Miles Inc. Procédé de préparation de micro-organismes secs, viables et stables pour l'alimentation et l'agriculture

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0559062A1 (fr) * 1992-03-06 1993-09-08 AGRA SOCIETA' DEL SEME S.r.l. Composition pour l'apprétage des semences, semences apprétées par cette composition et procédé par l'apprétage correspondant
WO2001005949A1 (fr) * 1999-07-15 2001-01-25 Unilever N.V. Procede de separation de membrane destine a purifier, a concentrer et a densifier les solutions d'enzymes avec des polyols
US6420333B1 (en) 2001-08-28 2002-07-16 Unilever Home & Personal Care Usa Division Of Conopco, Inc. Manufacture of capsules for incorporation into detergent and personal care compositions
US6730651B2 (en) 2001-08-28 2004-05-04 Unilever Home & Personal Care Usa Division Of Conopco. Inc. Concentrated stock of capsules for detergent or personal care compositions
CN101560491B (zh) * 2008-04-15 2012-01-04 中国科学院上海生命科学研究院 一种发光细菌及其在食品或水样总体生物毒性检测中的应用
KR20210031956A (ko) * 2018-07-20 2021-03-23 지멘스 에너지 글로벌 게엠베하 운트 코. 카게 나선형으로 감긴 도체 스트랜드를 갖는 장치 및 이러한 장치를 제조하기 위한 방법
KR102513237B1 (ko) 2018-07-20 2023-03-24 지멘스 에너지 글로벌 게엠베하 운트 코. 카게 나선형으로 감긴 도체 스트랜드를 갖는 장치 및 이러한 장치를 제조하기 위한 방법
WO2020049188A1 (fr) * 2018-09-07 2020-03-12 Nolivade Composition et procédé de conservation

Also Published As

Publication number Publication date
EP0437564A1 (fr) 1991-07-24
AU6033990A (en) 1991-02-22
GB8916152D0 (en) 1989-08-31
JPH04505262A (ja) 1992-09-17
CA2036391A1 (fr) 1991-01-15
AU5483594A (en) 1994-03-31

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