[go: up one dir, main page]

WO1991005360A1 - Spectrometre video a pixel - Google Patents

Spectrometre video a pixel Download PDF

Info

Publication number
WO1991005360A1
WO1991005360A1 PCT/US1990/005479 US9005479W WO9105360A1 WO 1991005360 A1 WO1991005360 A1 WO 1991005360A1 US 9005479 W US9005479 W US 9005479W WO 9105360 A1 WO9105360 A1 WO 9105360A1
Authority
WO
WIPO (PCT)
Prior art keywords
image
spectral
output
spectrometer system
slit
Prior art date
Application number
PCT/US1990/005479
Other languages
English (en)
Inventor
Sol Nudelman
Richard A. Buchroeder
Original Assignee
The University Of Connecticut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Connecticut filed Critical The University Of Connecticut
Publication of WO1991005360A1 publication Critical patent/WO1991005360A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
    • A61B3/14—Arrangements specially adapted for eye photography
    • A61B3/145—Arrangements specially adapted for eye photography by video means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G01J2003/2826—Multispectral imaging, e.g. filter imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/283—Investigating the spectrum computer-interfaced
    • G01J2003/2833—Investigating the spectrum computer-interfaced and memorised spectra collection

Definitions

  • This invention relates generally to diagnostic imaging systems such as employed in the medical technologies. More particularly, the present invention relates to imaging systems which can employ spectrometers to undertake spectral analysis of various structures and substances.
  • Spectrometers have long been employed for conducting spectral analysis of various substances and/or structures.
  • Conventional spectrometers employ a narrow input slit to essentially direct radiation from a point or an area-type source through the slit to a prism or a grating for dispersion into various wavelength components.
  • Recent advancements in digital processing technology have made possible the increasingly sophisticated and complex processing of spectral data.
  • the improvements in various medical imaging devices have resulted in high resolution images which can be employed fo medical diagnoses and analyses.
  • the invention in a preferred form is a vide pixel spectrometer having an input slit and an output opening.
  • the spectrometer includes a prism for dispersing radiation and various optical components providing a two dimensional spectral image.
  • the image corresponds to discrete positions along a line of an image sector which is projected through the spectrometer input slit.
  • An image acquisition device such as an endoscope can be employed for acquiring the image of the object to be examined.
  • An optical coupler transmits the image acquired by the acquisition device to the input jaws and the slit of the spectrometer.
  • a digital processor communicates with the output of the spectrometer for processing the spectral output and displaying spectral characteristics of the output for each of the series of pixels corresponding to the discrete positions of the input image along the slit.
  • a beam splitter may be incorporated into the optical coupler.
  • a video camera optically communicates with the beam splitter for viewing the acquired image. This image is also fed to an image processor.
  • a second camera may be employed for viewing the image projected onto the jaws of the slit. The second camera serves to identify the sector of the image passing through the slit.
  • the spectrometer in a preferred form employs a prism having a spectral response in the range of approximately 3000 Angstroms to 2 microns.
  • An intensifier is employed to intensify the signal received at the output of the spectrometer.
  • a positioner is also employed for variably positioning the image which is projected onto the slit.
  • the output spectra is processed and can be displayed on one or more video screens with information such as : the intensity spectrum for each wavelength from any individual pixel; the intensity spectrum from any two or more pixels displayed simultaneously; and a comparison of the spectrum from any two or more pixels.
  • a method for analyzing tissue for diagnostic purposes in accordance with the invention comprises acquiring an image of the tissue.
  • a sector of the image is projected through the input slit of a spectrometer.
  • the sector image is dispersed to provide a spectral image corresponding to discrete linear positions of the sector.
  • the spectral images are converted to digital data and processed to form enhanced images.
  • the spectral image has a two dimensional format which may be displayed on a video display.
  • Spectral images can be compiled in a library, displayed on a video display and compared to the spectral images from the tissue.
  • a video image of the tissue may be processed for feature characteristics such as texture, boundaries and growth patterns.
  • Such data can be correlated with spectral characteristics for improved diagnosis.
  • Such data can also be compiled in a library and subsequently compared with data from newly acquired imaging data for further processing and/or display.
  • An object of the invention is to provide a new and improved video pixel spectrometer.
  • Another object of the invention is to provide a new and improved video pixel spectrometer which is capable of being employed in conjunction with medical image acquisition systems.
  • a further object of the invention is to provide a new and improved video pixel spectrometer which is capable of providing simultaneous spectral analysis for a series of points along the line of an image.
  • Figure 1 is a general schematic diagram of a video pixel spectrometer system in accordance with the present invention
  • Figure 2 is a schematic view illustrating various components of the video pixel spectrometer system in accordance with the present invention
  • Figure 3 is an interior side elevational view, partly in schematic, partly in section, and partly in diagrammatic form with portions being removed, of a video pixel spectrometer in accordance with the present invention
  • Figure 4 is an interior top plan view, partly in schematic, partly in section, and partly in diagrammatic form with portions being removed, of the spectrometer of Figure 3;
  • Figure 5 is an interior side elevational view, partly in schematic, of the spectrometer of Figure 3;
  • Figure 6 is fragmentary interior rear view viewed from the left of Figure 3;
  • Figure 7 is a schematic diagram of the spectrometer system of Figure l including a schematic side elevational view of an optical coupler employed in the spectrometer system of Figure 1;
  • Figure 8 is an output display for the spectrometer system o Figure 1 illustrating a spectral plot from two pixel elements
  • Figure 9 is a second output display for the spectrometer system of Figure 1 illustrating a columnar spectral plot from tw pixel elements
  • Figure 10 is a third output display for the spectrometer system of Figure 1 illustrating spectra from a color test pattern.
  • a video pixel spectrometer (VPS) optical system in accordance with the present invention is generally designated by the numeral 10.
  • the VPS system 10 is adapted for acquiring an image of an object to be examined and for processing said image to provide a detailed examination of selected physical characteristics simultaneously with spectral analysis of the radiation emerging from a selected portion of the image.
  • the VPS system 10 has particular applicability in connection with medical diagnostic technologies although the invention is not limited to medical applications.
  • the invention is readily applicable to imaging systems employed in non-distructive testing.
  • the VPS system 10 comprises a medical image acquisition device 12 such as an endoscope, microscope, fundus camera or other device which generates an image of tissue and/or structure to be examined.
  • the image is transmitted to a video pixel spectrometer 14 via an optical coupler 16.
  • the optical output from the spectrometer is transmitted via a light intensifier 18 and an intensified video camera 28 to a digital image acquisition and processing system
  • Video cameras 24 and 26 are also connected with the optical coupler 16 to provide unprocessed and/or processed readily viewed images of the principal images acquired by the medical image acquisition device and readily viewed images of the selected image portion which is projected into the spectrometer 14.
  • the spectrometer 14 has a generally rectilinear housing 30 which houses the various optical components.
  • a pair of jaws 32 define an input slit 34 which in one embodiment has a length of approximately 10 millimeters.
  • the input slit 34 defines the image line to be analyzed.
  • the jaws are preferably coated with reflective surface to provide an optical path for communication with video camera 26.
  • An input mirror 36 behind slit 34 is pivotally mounted to a support bracket 35 which extends perpendicularly at the interior.
  • Mirror 36 directs the image toward a spherical mirror 38 which is supported by a bracket 40 secured to a support 41 mounted at the rear of the housing.
  • a fused silica prism 50 is mounted at the housing interior by means of a bracket 52. The image is reflected from mirror 38 through the dispersing prism 50 and onto aspheric mirror 54. The image is reflected from the aspheric mirror 54 back through the prism 50 to mirror 38.
  • the optical path then extends from mirror 56 to an outlet mirror 42 which directs the spectral output through the spectrometer outlet 44.
  • the aspheric mirror is mounted by a bracket 55 which is pivoted about pin 57.
  • a dial gage 60 includes a stem 62 which is moved by rotation of the aspheric mirror 54.
  • a micrometer driving screw 64 has an adjustment knob 65 which moves the aspheric mirror 54.
  • the dial gage 60 gives the location of the aspheric mirror 54 in millimeters for which a corresponding angular location can be determined. In one embodiment of spectrometer 14, the angular range of movement in the visible spectrum is approximately 1.22 degrees and corresponds to a 1.49 millimeter travel for the dial gage or the micrometer.
  • the aspheric mirror 54 can be selectively pivoted about pin 57 to select any wavelength as the central wavelength. This adjustment minimizes spherical aberration and coma.
  • the spectrometer was calibrated for a central wavelength of 632.8nm at minimum deviation angles through the prism.
  • the optical coupler 16 comprises a housing 70 having an input end 72 which couples with the medical image acquisition device 12.
  • a holder 75 such as illustrated in Figure 7, may be employed for mechanically coupling the image acquisition device 12 to the input end 72 of the coupler 16.
  • the input coupler may have a bayonet-type configuration.
  • the outlet coupler 74 may also have a bayonet configuration which couples to the spectrometer exterior surrounding the jaws 32.
  • Beam splitters 76 and 78 are interposed in an optical path within the housing 70 for providing an optical path to the video cameras 24 and 26. Alternately, the beam splitters may be opposite surfaces of one beam splitter unit.
  • Camera 24 records the image of the tissue examined as viewed from the image acquisition device.
  • Camera 26 records the image which is projected onto the jaws of the spectrometer and the location of the slit. Reflective coating applied to the jaws provides an optical path to the beam splitte 78.
  • One or both of the beam splitters may be removed from the coupler 16 to enhance the light intensity to the spectrometer.
  • the beam splitters 76 and 78 may each be readily mounted as a unit to the coupler housing 70 and/or dismounted therefrom in accordance with a given application.
  • a positioning mechanism 80 may be attached to provide a position adjustment wherein the portion of the tissue which is projected onto the jaws of the spectrometer may be selectively determined.
  • a micrometer knob 82 may be employed for implementing the positioning.
  • the images from camera 24 and 26 (as described hereafter) are employed in conjunction with manual operation of the positioning mechanism 80. Camera 26 is optional.
  • a single video camera system could be employed to accomplish both image acquisition and positioning of the acquired image.
  • the positioning may be accomplished by variably positioning the distal end of the image acquisition device and fixing the optical interface between the proximal end of the device and the coupler. Ordinarily positioning of the acquired image is accomplished by the latter procedure.
  • the length of the spectrometer input slit 34 approximately corresponds to one dimension of the two dimensional projection of the output spectrum through the outlet opening 44 of the spectrometer.
  • the output spectrum is essentially planar at the outlet opening 44 and generally perpendicular to the central optical axis through the opening 44.
  • Each point (pixel) of the input image along the slit has a corresponding point along an axis of the orthogonally oriented spectrometer output.
  • the spectral resolution is determined by the prism 50 and the various optical components as previously described for the spectrometer.
  • Each pixel element corresponds with a spectrum which is projected preferably in the vertical direction in Figure 2 with the short wavelength limit (approximately 3000 Angstroms) at the bottom of the graphical illustration and the near infrared (approximately 2 microns) at the top of the illustration.
  • the output spectrum is recorded by a third video camera 28 which is essentially one or more imaging devices depending upon the particular spectral range of concern for a given procedure and the intensity of the spectrum.
  • a low light level intensifier is essentially one or more imaging devices depending upon the particular spectral range of concern for a given procedure and the intensity of the spectrum.
  • the intensifier 18 which is used for low light level images is selected for a specific spectral response.
  • the intensifier 18 is coupled to the video camera 28. Intensifiers may also be employed with cameras
  • the low light intensifier 18 may be a proximity focused intensifier such as the Varian Model Proximity Focused
  • the output from the light intensifier 18 is input to the
  • the digital processing system 20 may comprise a digital image processor such as a Gould IP 9000 digital image processor or an IBM-type compatible computer. Software such as the "Gould Graphic User Menu" may be employed to distribute the digital images and to aid in processing.
  • the computer preferably communicates with the video console 22 having video displays Dl, D2, D3, D4 and D5.
  • the video displays can assume a number of configurations, examples of which are described as follows:
  • Video display Dl may be a display which provides an image screen depicting the principal image acquired by the medical image acquisition device.
  • the display Dl may be employed for determining the suitability of an image on a preliminary basis.
  • the depicted image may additionally include reflected radiation which is beyond the visible spectral range.
  • the video camera 24 may have sensors which are responsive to ultraviolet and near infrared so that the non-visible spectrum can be displayed in shades of grey or pseudo-color.
  • Video display D2 displays the image from the medical acquisition device which is projected onto the jaws 32 of the input slit 34 of the spectrometer. Naturally, the positioning of the portion of the image to be analyzed onto the spectrometer jaws must be accomplished in a precise manner.
  • the video camera 26 is trained on the jaws of the input slit which are coated so as to function as an efficient reflector.
  • the image which is acquired by camera 26 is shown on display D2.
  • the slit 34 essentially defines the analyzed sector of the image having a length corresponding to the slit length and a width corresponding to precisely the slit width.
  • the positioner 80 allows for the viewer or operator to position the image so that a given sector can be selected to fall on the entrance slit 34.
  • positioning is accomplished by maneuvering the image acquisition device.
  • any object such as a lesion within the overall image can be positioned so that the radiation passes through the spectrometer for a detailed spectral analysis.
  • Displays Dl and D2 are used to obtain the displayed image sector to be examined.
  • Video display D3 represents the output image from video camera 24 which is also transmitted to the digital image processing system 20.
  • Display D3 thus represents a processed image which is composed to account for processing techniques suc as edge enhancement, contrast enhancement, pseudo-color, real color, size discrimination, texture, magnification and other processing techniques which are appropriate for a given procedure.
  • Display D3 is preferably interactive to permit viewing during and after the processing of the image.
  • Video camera 24 thus requires sensitivity throughout the spectral rang of interest and also requires excellent performance specifications in terms of dynamic range plus spatial resolution which is sufficient to send a non-degraded image to the processing system 20.
  • Video display D4 represents the output spectrum from the spectrometer.
  • the spectrum may, for example, be illustrated wherein the longitudinal expanse of the slit 34 is projected along the horizontal axis.
  • the scale along the horizontal axis corresponds to positions along the entrance slit.
  • Spectral information is displayed vertically so that the ordinate scale corresponds to wavelength.
  • the data can be displayed as a matrix of numbers where each number corresponds to the number of recorded photons such as illustrated in Figure 9 for two selected pixels designated as pixel 100 and pixel 250.
  • the display can be illustrated in terms of shades of grey to provide visual comparison of spectra such as illustrated in the color test display pattern of Figure 10.
  • pseudo-color may also be employed to illustrate both visible and non-visible spectra for purposes of analysis.
  • Video display D5 represents the graphic plot of spectra which is derived for one or more pixels selected on the horizontal axis of display D4 such as illustrated in Figure 8 for pixels 100 and 250.
  • a physician may view an image on display Dl and observe a region of suspicion such as, for example, a lesion.
  • the image on display D2 is viewed to aid in positioning the desired image section of display Dl onto the slit 34 of the spectrometer.
  • the physician may then examine display D4 and note the relative spectrum changes from one pixel to another so that one pixel (pixel 100) may be located within the lesion and a second pixel (pixel 250) may be located just outside the lesion.
  • the spectrum from each pixel is then plotted simultaneously on display D5 revealing their similarities and differences.
  • a library of spectra which is associated with known normal and disease tissue.
  • a library is compiled into a database and easily made available for comparison.
  • the library is accessed or displayed on display D5 when required.
  • spectral recorded in medical procedures are broad band.
  • broa band spectra will often not be sufficient to provide readily apparent characteristics suitable for identifying many kinds of diseased tissue with a simple spectral signature.
  • the spectra must be processed to reveal any subtle indentifying features.
  • the subtle changes i spectra will have to be correlated with various physical characteristics of the tissue to ascertain a final diagnosis. Such characteristics can include texture, growth patterns plus boundary shapes and conditions.
  • the spatial resolution which is achievable by the VPS system 10 is a function of the size of the pixel element.
  • the size of the pixel element is a function of the performance of the components of the VPS system including the image acquisition device 12, the spectrometer 14 and the digital image acquisition system 20.
  • Each raster line of the image may be subdivided into as many pixels as the digital computer system will allow. For example, for a 2048 x 2048 matrix system, a 6 to 10 inches linear section of an imaged breast could be composed of 2,048 pixels or alternately a few millimeters of linear sector from a small tissue sample could be composed of 2,048 pixels. Naturally, a number of different pixel matrices systems could be employed. Ordinarily, a 512 or 1,024 pixel format would be sufficient for most diagnostic practices. A 2,048 pixel format could be implemented with procedures which need a greater spatial resolution and/or span large areas.
  • the spectral band for the spectrometer 14 can be quite broad. A broad spectral band would be required for a general medical diagnostic applicator. In a preferred embodiment, the spectral band of the spectrometer ranges from approximately 3,000 Angstroms to approximately 2 microns. This could be accomplished with a quartz prism. A grating (not illustrated) may be employed instead of a prism when only certain selected spectral regions are required for analysis.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Biophysics (AREA)
  • Surgery (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Multimedia (AREA)
  • General Physics & Mathematics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Spectrometry And Color Measurement (AREA)

Abstract

Un système de spectromètre vidéo à pixels utilise un dispositif (12) d'acquisition d'image et un spectromètre (14) de manière à créer une visualisation vidéo des caractéristiques spectrales d'un secteur d'image pour une série de positions discrètes dudit secteur de l'image. La sortie du spectromètre (14) est transformée en données numériques et traitée afin de réaliser des visualisations vidéo diverses (D1, D2, D3, D4 et D5) desdites caractéristiques spectrales. On utilise également des caméras (24, 26 et 28) de manière à obtenir des images pour le traitement de particularités physiques diverses ainsi que pour faciliter le positionnement de l'image d'entrée.
PCT/US1990/005479 1989-09-26 1990-09-26 Spectrometre video a pixel WO1991005360A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41291989A 1989-09-26 1989-09-26
US412,919 1989-09-26

Publications (1)

Publication Number Publication Date
WO1991005360A1 true WO1991005360A1 (fr) 1991-04-18

Family

ID=23635006

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/005479 WO1991005360A1 (fr) 1989-09-26 1990-09-26 Spectrometre video a pixel

Country Status (1)

Country Link
WO (1) WO1991005360A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5329352A (en) * 1991-04-12 1994-07-12 Bayer Aktiengesellschaft Spectroscopically correlated light scanning microscopy
WO1996011386A1 (fr) * 1994-10-10 1996-04-18 Soliton Elektronik Ab Procede de representation a base spectrale concernant des elements d'image disposes sur une ligne
EP0780671A1 (fr) * 1995-12-21 1997-06-25 Shimadzu Corporation Spectrophotomètre et colorimètre
WO1998030873A1 (fr) * 1997-01-14 1998-07-16 Inphocyte, Inc. Systeme et methode de cartographie de la distribution de cellules normales et anormales dans des sections de tissus
EP0830564A4 (fr) * 1995-12-12 1999-11-10 Spectral Diagnostic Ltd Procedes de bio-imagerie spectrale s'appliquant a la recherche biologique, aux diagnostics medicaux et en therapie
EP1387154A1 (fr) * 2002-07-31 2004-02-04 Galileo Avionica S.p.A. Spectromètre à large bande avec un objectif comprenant un miroir de correction asphérique et un élément dispersif traversé deux foix du faisceau incident
US6760106B2 (en) * 2001-03-02 2004-07-06 Ernest A. Carroll Method of and apparatus for registering a single dimensional image with a two dimensional reference image

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3260180A (en) * 1964-01-27 1966-07-12 Beckman & Whitley Inc Spectral dispersion attachment for cameras
US3363525A (en) * 1964-02-17 1968-01-16 Beckman & Whitley Inc Photography of spectral dispersion
US4556057A (en) * 1982-08-31 1985-12-03 Hamamatsu Tv Co., Ltd. Cancer diagnosis device utilizing laser beam pulses
US4718412A (en) * 1984-09-13 1988-01-12 Nesbitt William R Disposable cervical immobilization means
US4852579A (en) * 1987-04-20 1989-08-01 Karl Storz Endoscopy Gmbh And Company Photocharacterization and treatment of normal abnormal and ectopic endometrium
US4917492A (en) * 1988-06-21 1990-04-17 Hamamatsu Photonics Kabushiki Kaisha Spectrum measuring apparatus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3260180A (en) * 1964-01-27 1966-07-12 Beckman & Whitley Inc Spectral dispersion attachment for cameras
US3363525A (en) * 1964-02-17 1968-01-16 Beckman & Whitley Inc Photography of spectral dispersion
US4556057A (en) * 1982-08-31 1985-12-03 Hamamatsu Tv Co., Ltd. Cancer diagnosis device utilizing laser beam pulses
US4718412A (en) * 1984-09-13 1988-01-12 Nesbitt William R Disposable cervical immobilization means
US4852579A (en) * 1987-04-20 1989-08-01 Karl Storz Endoscopy Gmbh And Company Photocharacterization and treatment of normal abnormal and ectopic endometrium
US4917492A (en) * 1988-06-21 1990-04-17 Hamamatsu Photonics Kabushiki Kaisha Spectrum measuring apparatus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AMERICAN LABORATORY, Vol. 11, No. 3, March 1979, WOOD et al., "T.V. Direct Reading Spectrometer", see pages 16-27. *
APPLIED SPECTROSCODY, Vol. 29, No. 4, 1975, WOOD et al., "Computerized T.V. Spectrometer for Emission Anaylsis", see pages 310-315. *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5329352A (en) * 1991-04-12 1994-07-12 Bayer Aktiengesellschaft Spectroscopically correlated light scanning microscopy
WO1996011386A1 (fr) * 1994-10-10 1996-04-18 Soliton Elektronik Ab Procede de representation a base spectrale concernant des elements d'image disposes sur une ligne
EP0830564A4 (fr) * 1995-12-12 1999-11-10 Spectral Diagnostic Ltd Procedes de bio-imagerie spectrale s'appliquant a la recherche biologique, aux diagnostics medicaux et en therapie
EP0780671A1 (fr) * 1995-12-21 1997-06-25 Shimadzu Corporation Spectrophotomètre et colorimètre
US5706083A (en) * 1995-12-21 1998-01-06 Shimadzu Corporation Spectrophotometer and its application to a colorimeter
WO1998030873A1 (fr) * 1997-01-14 1998-07-16 Inphocyte, Inc. Systeme et methode de cartographie de la distribution de cellules normales et anormales dans des sections de tissus
US6760106B2 (en) * 2001-03-02 2004-07-06 Ernest A. Carroll Method of and apparatus for registering a single dimensional image with a two dimensional reference image
EP1387154A1 (fr) * 2002-07-31 2004-02-04 Galileo Avionica S.p.A. Spectromètre à large bande avec un objectif comprenant un miroir de correction asphérique et un élément dispersif traversé deux foix du faisceau incident

Similar Documents

Publication Publication Date Title
US8126205B2 (en) Sample imaging and classification
US4579430A (en) Method and apparatus for forming an image of the ocular fundus
US10337995B2 (en) Systems and methods for oblique laser scanning
CN102892348B (zh) 多光谱光子成像的方法和装置
RU2288636C2 (ru) Способ и системы для определения параметров и картографирования поражений ткани
US8177362B2 (en) Optical image measurement device
JP7246540B2 (ja) 涙液層厚み測定装置及び方法
EP0167877A2 (fr) Appareil destiné à visualiser des sections de l'oeil humain
KR20030033177A (ko) 형광 내시경 장치 및 그 장치를 이용한 진단부위 조상 방법
WO2008027927A2 (fr) Microscopie spectroscopique avec analyse par image
JP2014526700A (ja) 医療診断支援のための画像評価装置、医療診断支援システムおよびその操作制御方法
CN109342328A (zh) 一种内置扫描型显微高光谱成像系统及成像方法
JP5424108B2 (ja) ラマンイメージング装置
CN113490450A (zh) 用于检查头发样本的设备和方法
KR100849921B1 (ko) 목적물의 스펙트럼 방사의 공간 분포를 측정하는 장치
CN118490161A (zh) 一种眼底成像的高光谱检测装置及方法
WO1991005360A1 (fr) Spectrometre video a pixel
JP2558864B2 (ja) 分光解析装置
Benedetti et al. Confocal‐line microscopy
DE4410888A1 (de) Anordnung zur Durchstrahlung von menschlichem Gewebe
JP4237300B2 (ja) 光学顕微装置
JPH0820309B2 (ja) 分光演算装置
DE68915151T2 (de) Halbleiter Mikroskop.
Yang Development of a handheld fiber-optic probe-based raman imaging instrumentation: raman chemlighter
CN118591749A (zh) 自动化样品分析中的表面感测

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE

NENP Non-entry into the national phase

Ref country code: CA