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WO1991006369A2 - Chambre d'equilibrage de la temperature, notamment du contenu d'une lame de micro-titrage - Google Patents

Chambre d'equilibrage de la temperature, notamment du contenu d'une lame de micro-titrage Download PDF

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Publication number
WO1991006369A2
WO1991006369A2 PCT/AT1990/000107 AT9000107W WO9106369A2 WO 1991006369 A2 WO1991006369 A2 WO 1991006369A2 AT 9000107 W AT9000107 W AT 9000107W WO 9106369 A2 WO9106369 A2 WO 9106369A2
Authority
WO
WIPO (PCT)
Prior art keywords
temperature
heating
temperature control
plate
chamber according
Prior art date
Application number
PCT/AT1990/000107
Other languages
German (de)
English (en)
Other versions
WO1991006369A3 (fr
Inventor
Karl Puchegger
Josef Atzler
Original Assignee
Slt-Labinstruments Gesellschaft M.B.H.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Slt-Labinstruments Gesellschaft M.B.H. filed Critical Slt-Labinstruments Gesellschaft M.B.H.
Priority to FI913192A priority Critical patent/FI913192A7/fi
Publication of WO1991006369A2 publication Critical patent/WO1991006369A2/fr
Publication of WO1991006369A3 publication Critical patent/WO1991006369A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Definitions

  • Temperature control chamber especially for temperature control of the contents of a microtitration plate
  • the invention relates to a temperature control chamber, in particular for temperature control of the content of a microtitration plate with a housing with a base plate designed as a temperature control plate, which has a controllable heat exchanger, which is preferably formed by an electrical, plate-shaped temperature control element and one Cover cap.
  • Such temperature chambers are used in particular in the photometric measurement of blood samples.
  • temperature devices are required which are suitable for microcuvettes and conventional microtitration plates in the existing measuring devices, e.g. Photometers can be used.
  • Temperature control chambers are known from the reading device, according to the more recent state of the art these are advantageously arranged directly next to or in the same, so that there is no change in temperature of the liquid contained in the microtitration plate when the latter is fed to the reading device.
  • the temperature control units require a quick warm-up and a corresponding temperature constancy (e.g. 37 degrees C. +/- 0.3 degrees C.).
  • a corresponding temperature constancy e.g. 37 degrees C. +/- 0.3 degrees C.
  • the object of the invention is to provide a temperature control device of the type mentioned at the outset which ensures better and more uniform heating or cooling of the contents of microtitration plates. It should also be possible to keep the temperature constant for as long as possible. This allows measurements to be carried out over a longer period of time, e.g. B. in the form of a series of measurements with several individual measurements at a distance of z. B. five minutes each.
  • An embodiment of the invention provides that the bottom plate has two heat exchangers, one heat exchanger surrounding the other and being assigned to the edge of the microtiter plate.
  • tempering means warming up because, for example, blood samples have to be warmed from room temperature (approx. 20 ° C.) or refrigerator temperature (approx. 4 ° C.) to body temperature (approx. 37 ° C.). In countries with high air temperature, however, the temperature control chamber can also be used for cooling.
  • the perforating chamber as a heating chamber, since this is the more frequently occurring case. It is advantageously provided that the heat exchangers are formed by heating circuits, for example by electrical resistance heating elements.
  • the construction according to the invention makes it possible, for example, to set the inner heating circuit to a higher temperature than the outer heating circuit during heating, so that a compensation is created for the cavities of the microtitration plate and all cavities are heated essentially the same regardless of their position become.
  • the outer heating circuit is set to a temperature which is increased with respect to the inner heating circuit.
  • a further exemplary embodiment of the invention provides that a heating circuit is assigned to the base plate and a heating circuit to the cover, the cover being designed like a flap and covering the microtitration plate like a bell.
  • the heat exchangers are formed by electrical temperature control elements which are embedded in foils or formed by heat-conducting plates.
  • a separation joint is provided in the film or plate between two heat exchangers.
  • the heat exchangers can be, for example, heating circuits of electrical resistance heaters.
  • the invention is not intended to be restricted to electrical temperature control elements, it could also be heated with pipes which carry a liquid, the construction with electrical temperature control elements nevertheless has constructive advantages, since such heaters are relatively are to be produced professionally.
  • the parting line between the foils results in better thermal delimitation of the heating and cooling circuits.
  • one or more temperature sensors are provided which are in contact with this when the microtiter plate is inserted; the starting temperature of the plate can be determined via these sensors or any temperature change during the reading process can be determined and via a corresponding one Control of the temperature control unit can be compensated.
  • the method for heating microtitration plates provides that the starting temperature and the target temperature of the contents of a microtitration plate are entered in a computer and, after taking the filling quantity into account, the amount of energy required to achieve the target temperature is calculated.
  • the method according to the invention makes it possible to significantly shorten the temperature setting, ensuring that the contents of the microtitration plate are not heated above the specified target temperature.
  • the heating-up time is calculated on the basis of the filling quantity.
  • the heating temperature can be chosen empirically.
  • FIG. 1 shows a diagram of a reading device for microtitration plates with a temperature chamber according to the invention
  • FIG. 2 shows a schematic top view of the device according to FIG. 1
  • FIG. 3 shows a longitudinal section through the temperature chamber
  • FIG. 4 shows a cross section through the temperature chamber
  • FIG. 5 shows the support of the outer edge of the cover in section
  • FIG. 6 shows a plan view of a temperature control element according to the invention, which FIG. 7 shows a temperature diagram and
  • FIG. 8 shows a circuit for performing the method according to the invention.
  • the actual reading device 1 and the temperature control chamber 2 are arranged directly next to one another and accommodated in a common housing 3.
  • the temperature control chamber 2, whose dimensions are adapted to a microtitration plate 4, is covered by a cover 5, which is designed as a flap-like cover.
  • the microtitration plate 4 rests on a slide 6 which is guided on rods 7 and moves back and forth between the temperature control chamber 2 and the reading device 1.
  • the microtitration plate 4 is therefore inserted into the temperature control chamber 2 and the cover 5 is closed.
  • the temperature chamber 2 is heated or cooled until the contents of the cavities in the microtitration plate 4 have reached the desired target temperature (generally 37 ° C.), whereupon the microtitration plate 4 is not exposed to room temperature, which in turn causes a change in temperature
  • the desired target temperature generally 37 ° C.
  • a temperature control film 8 is arranged on the bottom of the temperature chamber 2.
  • the tempering film 8 is usually not necessarily a heating film.
  • the tempering foil 8 is covered by an aluminum foil 9. Mainly, the microtitration plate 4 is heated from below by the temperature control film 8.
  • a temperature control film 10 is also provided on the underside of the cover 5, which in turn is covered with an aluminum film 9.
  • the tempering film 10 is assigned a separate heating or cooling circuit.
  • the lower temperature control film 8 is divided and has an inner heat exchanger 11 and an outer heat exchanger 12.
  • heating or cooling could also be installed in which a fluid is the heat transfer medium.
  • Ceramic or metallic plates with an applied semiconductor layer can also be used.
  • cooling devices can be used.
  • the heating chamber 2 is now provided with three heating circuits.
  • the heating circuits of the heat exchangers 11 and 12 are controlled in such a way that, during the heating phase, the heat exchanger 11 emits more heat than the heat exchanger 12. This ensures uniform heating of all cavities in the microtitration plate.
  • the heating circuit of the heat exchanger 12 After reaching the target temperature, the heating circuit of the heat exchanger 12 emits more heat than the heating circuit of the heat exchanger 11 over the duration of the stable phase, in which it is only important to keep the temperature constant, in order to compensate for the increased heat dissipation of the microtitration plate 4 at the edges .
  • sensors 13, 14 are also provided, which bear against the microtitration plate 4.
  • the temperatures which are measured by the sensors 13, 14 and passed on to the computer 18 correspond at least approximately to the temperature which really prevails in the interior of an adjacent cavity of the microtitration plate 4.
  • the cover 5 projects with an edge web 15 into a groove 16 in the side wall 17 of the heat chamber 2. This forms an obstacle that extends the path of the air flow.
  • the temperature values can be entered manually into the computer 16 or can also be detected by sensors and passed on directly.
  • the filling quantity of the liquid contained in the cavitates is entered into the computer 18, from which the computer calculates the amount of energy required for heating or cooling the contents of the microtitration plate 4 to the desired target temperature or the warming-up or cooling-down period.
  • the warm-up time is given on the abscissa in minutes, while the ordinate shows the temperature.
  • the curve FT shows the course of the temperature of the liquid contained in the cavities of the microtitration plate 4, the curve WT shows the course of the temperature of a heat exchanger 9, 11, 12.
  • Target temperature of z. B. 37 ° C can be reached quickly, without running the risk that the contents of the micro plate 4 ever, apart from a predetermined tolerance range, heats up above this value.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Control Of Temperature (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)

Abstract

Une chambre d'équilibrage de la température du contenu d'une lame (4) de micro-titrage comprend une plaque de fond qui forme une plaque d'équilibrage de la température et un couvercle (5), ainsi qu'au moins deux échangeurs de chaleur (9, 11, 12) séparément réglables. De préférence, la plaque de fond comprend deux échangeurs de chaleur (11, 12), dont le premier (12) entoure le second et est relié au bord de la lame (4) de micro-titrage.
PCT/AT1990/000107 1989-11-02 1990-11-02 Chambre d'equilibrage de la temperature, notamment du contenu d'une lame de micro-titrage WO1991006369A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI913192A FI913192A7 (fi) 1989-11-02 1990-11-02 Lämpötilantasauskammio, erityisesti mikrotitrauslevyn sisällön lämpöti lan tasaamiseksi

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT252489A AT394323B (de) 1989-11-02 1989-11-02 Temperierkammer, insbesondere fuer die temperierung des inhalts einer mikrotitrationsplatte, und verfahren zur steuerung eines heizkreises hiefuer
ATA2524/89 1989-11-02

Publications (2)

Publication Number Publication Date
WO1991006369A2 true WO1991006369A2 (fr) 1991-05-16
WO1991006369A3 WO1991006369A3 (fr) 1991-08-08

Family

ID=3535818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT1990/000107 WO1991006369A2 (fr) 1989-11-02 1990-11-02 Chambre d'equilibrage de la temperature, notamment du contenu d'une lame de micro-titrage

Country Status (4)

Country Link
EP (1) EP0451242A1 (fr)
JP (1) JPH04502580A (fr)
AT (1) AT394323B (fr)
WO (1) WO1991006369A2 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0488769A3 (en) * 1990-11-29 1993-05-26 Perkin-Elmer Cetus Instruments Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
DE4217868A1 (de) * 1992-05-29 1993-12-02 Univ Schiller Jena Temperierbare Multiküvette
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
US6372486B1 (en) 1998-11-30 2002-04-16 Hybaid Limited Thermo cycler
US6767512B1 (en) 1996-11-08 2004-07-27 Eppendorf Ag Temperature-regulating block with temperature-regulating devices
DE19655141B4 (de) * 1996-11-08 2005-04-07 Eppendorf Ag Gradienten-Temperierblock für Laborthermostaten
DE19655282B4 (de) * 1996-11-08 2005-04-07 Eppendorf Ag Temperierblock mit Temperiereinrichtungen
DE102009015869A1 (de) 2009-04-01 2010-10-21 Schneckenburger, Herbert, Prof. Dr. Vorrichtung zum Temperieren von Mikrotiterplatten

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4492466B2 (ja) * 2005-07-11 2010-06-30 株式会社島津製作所 試料恒温装置
DE102010019232B4 (de) * 2010-05-03 2013-06-27 Eppendorf Ag Kondensatvermeidungshaube

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5821566A (ja) * 1981-07-31 1983-02-08 Fuji Photo Film Co Ltd インキユベ−タ
DE3214317A1 (de) * 1982-04-19 1983-12-15 Behringwerke Ag, 3550 Marburg Mikrotiterplatte
IL71131A (en) * 1984-03-02 1988-09-30 Product Advanced Ltd Method and apparatus for heating and/or cooling objects simultaneously at different preselected temperatures
DE3441179C2 (de) * 1984-11-10 1987-02-26 Dynatech Deutschland GmbH, 7306 Denkendorf Temperiereinrichtung für Mikro-Küvettenanordnungen, insbesondere für Mikrotitrationsplatten
DE3513385A1 (de) * 1985-04-15 1986-10-16 Devappa Dr.Ing. Zinsser & Ing. K.Prestl, 8420 Kelheim Vorrichtung zum temperieren
GB8814962D0 (en) * 1988-06-23 1988-07-27 Lep Scient Ltd Biochemical reaction machine
NL8803052A (nl) * 1988-12-13 1990-07-02 Interconnection B V Verwarmde microtiterplaat.
EP0388159A3 (fr) * 1989-03-15 1991-06-12 Seiko Instruments Inc. Dispositif pour le scellement d'un liquide dans des cavités

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015534A (en) * 1990-11-29 2000-01-18 The Perkin-Elmer Corporation PCR sample tube
US5475610A (en) * 1990-11-29 1995-12-12 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
EP0812621A1 (fr) * 1990-11-29 1997-12-17 The Perkin-Elmer Corporation Réaction de polymérase en chaîne automatisée
US5710381A (en) * 1990-11-29 1998-01-20 The Perkin-Elmer Corporation Two piece holder for PCR sample tubes
EP0488769A3 (en) * 1990-11-29 1993-05-26 Perkin-Elmer Cetus Instruments Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
DE4217868A1 (de) * 1992-05-29 1993-12-02 Univ Schiller Jena Temperierbare Multiküvette
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
DE19646115C2 (de) * 1996-11-08 2000-05-25 Eppendorf Geraetebau Netheler Verwendung von Temperiereinrichtungen zur Temperierung eines Temperierblockes
US6767512B1 (en) 1996-11-08 2004-07-27 Eppendorf Ag Temperature-regulating block with temperature-regulating devices
DE19655141B4 (de) * 1996-11-08 2005-04-07 Eppendorf Ag Gradienten-Temperierblock für Laborthermostaten
DE19655282B4 (de) * 1996-11-08 2005-04-07 Eppendorf Ag Temperierblock mit Temperiereinrichtungen
DE19655141C5 (de) * 1996-11-08 2013-12-05 Eppendorf Ag Gradienten-Temperierblock für Laborthermostaten
US6372486B1 (en) 1998-11-30 2002-04-16 Hybaid Limited Thermo cycler
DE102009015869A1 (de) 2009-04-01 2010-10-21 Schneckenburger, Herbert, Prof. Dr. Vorrichtung zum Temperieren von Mikrotiterplatten

Also Published As

Publication number Publication date
ATA252489A (de) 1991-09-15
AT394323B (de) 1992-03-10
WO1991006369A3 (fr) 1991-08-08
EP0451242A1 (fr) 1991-10-16
JPH04502580A (ja) 1992-05-14

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