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WO1992001383A1 - Procede de production de compositions contenant des produits pesticides biosynthetiques et utilisation des compositions ainsi obtenues - Google Patents

Procede de production de compositions contenant des produits pesticides biosynthetiques et utilisation des compositions ainsi obtenues Download PDF

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Publication number
WO1992001383A1
WO1992001383A1 PCT/DK1991/000213 DK9100213W WO9201383A1 WO 1992001383 A1 WO1992001383 A1 WO 1992001383A1 DK 9100213 W DK9100213 W DK 9100213W WO 9201383 A1 WO9201383 A1 WO 9201383A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture
tannin
compositions
paste
microorganisms
Prior art date
Application number
PCT/DK1991/000213
Other languages
English (en)
Inventor
René Detroz
Michel Caprasse
Lucien Charmoille
Original Assignee
Novo Nordisk A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk A/S filed Critical Novo Nordisk A/S
Priority to BR919105844A priority Critical patent/BR9105844A/pt
Publication of WO1992001383A1 publication Critical patent/WO1992001383A1/fr
Priority to CS92852A priority patent/CS85292A3/cs

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus

Definitions

  • the present invention relates to a process for the production of compositions containing biosynthetic pesticidal products obtained from microorganisms of the
  • the process for obtaining biosynthetic pesticidal products from microorganisms generally comprises the following principal steps:
  • the collection of the culture is a delicate step in this process; in fact this step, which consists principally in separating the culture in the form of biomass and the culture liquid, is one of the causes of loss of product and therefore of the poor yield generally- obtained.
  • the active material of the pesticidal product is a sensitive compound by virtue of its biological structure which is easily denatured if certain conditions are not respected, such as, in particular, the temperature, the pH, the solvents and the additives.
  • the poor yields observed during harvesting are due in particular to the poor separation of numerous constituents of the culture, which is viscous to a greater or lesser extent, to mechanical effects in the separation which cause the degradation of the active material of the pesticidal product, and therefore involve a fall in its biological activity, to the addition of adjuvants which affect the stability of the composition and/or its physical appearance, such as the fluidity of the paste, the separation of the paste or of the cream during storage, and the dispersion of the composition in water.
  • composition is formulated in the form of paste.
  • This formulation is advantageous in certain cases because it avoids the drying step which degrades the product to a greater or lesser extent and which is costly but is necessary for long-term preservation.
  • the present invention has the aim of obtaining compositions which no longer have these disadvantages.
  • it has the aim of obtaining compositions having a biological activity which is as high as possible and obtained in an economic fashion, that is to say avoiding the losses in yield at all levels, as well as from the point of view of quantity as of quality, and of biological activity, although being stable and having a satisfactory physical appearance.
  • the compositions according to the invention have a particularly high biological activity.
  • the invention relates to a process for the production of compositions containing biosynthetic pesticidal products obtained from micro ⁇ organisms of the Bacillaceae family and at least one tannin or a tannin derivative, this process comprising the following steps:
  • the microorganism in a first step, the microorganism is cultured in a culture medium to obtain an abundant culture of this microorganism, 2) in a second step, a quantity of tannin or a tannin derivative is added to the culture obtained,
  • the water is separated from the culture containing the tannin or the tannin derivative in order to obtain a paste
  • the preferred process according to the invention is that where tannic acid is added to the culture during the second step.
  • the biosynthetic pesticides obtained from the Bacillaceae family can be found in the concentrated compositions in very different forms. Thus, they can be present in the form of the organisms themselves at any stage of their development, including possible vegetative forms, as they are, in association with their culture medium, in a completely or partially lysed form, in a completely or partially sporulated form, in a form having partially or completely released the spores by various means such as bacterial autolysis, or in the form of products which are spontaneously excreted by the organisms, such as exotoxins, in the form of products which are extracted from these organisms, such as endotoxins, by any method known per se, involving or not involving lysis of the organism concerned, in the form of products possibly liberated by the organisms in the course of certain steps of their development (crystals or endotoxins associated or not associated with spores) or alternatively in several of these forms simultaneously.
  • microorganisms of the Bacillaceae family highly suitable in general as a source of biosynthetic pesticides the microorganisms of the genus Bacillus such as Bacillus thuringiensis, Bacillus sphaericus, Bacillus popilliae, Bacillus cereus, Bacillus larvae, Bacillus lentimorbus, Bacillus friciosensis and Bacillus penetrans (Pasteuria) are used.
  • Bacillus sphaericus and Bacillus thuringiensis of all serotypes these including bacteria resulting from genetic transformation, and more particularly Bacillus sphaericus of serotypes la, 2a2b, 2a2c, 5a5b, 6, 25, 26a26b, 26a26c and Bacillus thuringiensis of serotypes 1, 3a, 3a3b, 4, 6, 7, 8, 8a8b, 9 , 10 and 14.
  • the various types of Bacillus thuringiensis and of Bacillus sphaericus can be used in the compositions according to the invention in the form of the microorganisms themselves, as such or lysed, of their spores, of endotoxins or of crystals associated or not associated with their spores or in several of these forms.
  • compositions obtained according to the invention can be present in the form of a suspension of solid particles of biosynthetic pesticidal products in a liquid or in the form of a solid such as a powder or a ' granulate.
  • compositions obtained according to the invention may in addition contain various other additives of which the nature and the function depend on the use of these compositions and which are known by the person skilled in the art.
  • compositions obtained according to the invention can likewise contain residues from the production of the biosynthetic pesticide.
  • compositions obtained according to the invention are present in the form of suspensions in a liquid
  • these suspensions in general contain from 10 to 45% by weight and most frequently from 15 to 40% by weight of biosynthetic pesticidal products.
  • the liquid must be inert with respect to the biosynthetic pesticides and it must especially not deactivate them or provoke their coagulation or any other physical alteration.
  • the liquid can be formed by a single solvent or by a mixture of solvents. In general, the solvent contains water.
  • the solvent contains at least 80% and most frequently at least 95% by weight of water.
  • the liquid present in the composition according to the invention is formed essentially of water.
  • the necessary quantity of tannin or of tannin derivatives is between 0.05 and 4% by weight of the culture, usually between 0.1 and 3% and preferably between 0.25 and 2%.
  • the third step which consists in separating the water from the culture, is carried out by customary techniques for the separation of water such as centrifugation, ultrafiltration, precipitation by addition of a non-solvent such as acetone, filtration or flocculation so as to obtain a paste. Preferably, it is carried out by centrifugation and/or ultrafiltration.
  • the culture medium is taken, for- example, at the end of the sporulation phase.
  • Another process consists in separating fractions rich in endotoxins or in spores or in these two products simultaneously from the culture medium of the microorganisms generating biosynthetic pesticidal products at the end of the sporulation phase. These fractions are then treated according to the same techniques as those used for the treatment of the pastes.
  • the paste thus obtained is dried by conventional techniques such as spray drying, freeze drying or drying in a drum so as to obtain solid particles in general containing less than 12% and preferably less than 7% of water.
  • the solid pesticide particles can be dispersed in a liquid phase which preferably contains water and which additionally contains the tannin or the tannin derivative as well as the other possible additives, to form a cream.
  • the present invention likewise relates to a process for the use of the compositions.
  • compositions according to the invention can be used as pesticides and more particularly as agents to eliminate the insects at any stage of their development. To do this, they are sprayed onto the infested surfaces, plants, rivers, streams and any stretch of water according to all methods known per se such as manual spraying, mechanical spraying and more particularly aerial spraying. They can in particular be sprayed onto surfaces infested by insects and more particularly by Lepidopter, Dipter, Coleopter, Aphanipter, Orthopter, Isopter and Homopter.
  • the doses to be used depend on the biosynthetic - 1 -
  • compositions according to the invention can be sprayed as such when they are present in the form of suspensions. They can likewise be dispersed, diluted or emulsified in a diluent such as water or an organic diluent. In this case, they are diluted by 1 to 300 times, usually 2 to 120 times and preferably 4 to 60 times their volume of diluent.
  • soya flour containing approximately 52% of proteins 250 g of hydrolysed starch, 20 g of KH 2 P0 ⁇ ,, 20 g of 2 HP0 4 , 3.2 g of MgS0.7H 2 0, 0.12 g of MnS0 ⁇ .H 2 0, 0.14 g of ZnS0 4 .7H 2 0, 0.2 g of Fe 2 (S0 3 , 1.5 g of CaCl 2 .H 2 0 and 101 of water are introduced into a sterilisable 15 1 BIOLAFFITE fer enter equipped with a stirrer, a pH probe, an oxygen probe and an aeration system.
  • This culture medium is mixed until it is homogeneous; the pH of this medium is brought to 7.
  • This culture medium is sterilised at 120°C for 30 minutes.
  • the culture medium is cooled to bring it to a temperature of 30°C and 1 1 of a culture of Bacillus thuringiensis serotype 14 (israelensis variety) in the growth phase is introduced into the fermenter in a sterile manner.
  • the fermenter is stirred at 450 revolutions per minute and aerated at 0.4 wm (volume of air per volume of medium per minute) .
  • Bacillus thuringiensis is present in the form of a partially lysed sporulated bacterium.
  • This culture contains 1.1 x 10 9 spores per ml, its pH is 7.5 and its dry matter is 3 g per 1.
  • insecticidal activity of this culture is measured with the aid of Aedes aegypti according to the biological titration method of H. de Barjac and - 8 -
  • the product is then concentrated by centrifugation to give a paste containing approximately 12% of dry matter.
  • This paste has an insecticidal activity of 2800 U/mg +/- 20%.
  • This paste is fluid and stable in the course of time.
  • a culture of Bacillus thuringiensis serotype 14 is produced as in Example 1.
  • a paste identical to that of Example 1 is then prepared with the exception of the addition of tannin.
  • the paste obtained has an insecticidal activity of 1500 U/mg +/- 20%.
  • Example 3R (comparison) A culture of Bacillus thuringiensis serotype 14 is produced as in Example 1. A paste identical to that of Example 1 is then prepared with the exception of the addition of tannin which is replaced by the addition of 0.1% of silica, trademark AEROSIL R 972 silica from the Company DEGUSSA .
  • the insecticidal activity of the paste obtained amounts to 1300 U/mg +/- 20%.
  • the paste is compact and does not disperse in water.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Escalators And Moving Walkways (AREA)

Abstract

L'invention se rapporte à un procédé qui permet de produire des compositions contenant un produit pesticide biosynthétique à partir de microorganismes de la famille des Bacillaceae et à partir d'acides tels qu'au moins de l'acide tannique. Les compositions ainsi obtenues sont efficaces comme agents de lutte contre les insectes.
PCT/DK1991/000213 1990-07-23 1991-07-18 Procede de production de compositions contenant des produits pesticides biosynthetiques et utilisation des compositions ainsi obtenues WO1992001383A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BR919105844A BR9105844A (pt) 1990-07-23 1991-07-18 Processo para a producao de composicoes contendo produtos pesticidas biossinteticos e para eliminar insetos
CS92852A CS85292A3 (en) 1990-07-23 1992-03-20 Process for preparing preparations containing biosynthetic pesticidalproducts and the use of the products obtained in such a manner

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE9000738A BE1004492A3 (fr) 1990-07-23 1990-07-23 Compositions contenant des produits pesticides biosynthetiques et au moins un tannin, procedes pour leur production et leur utilisation.
BE9000738 1990-07-23

Publications (1)

Publication Number Publication Date
WO1992001383A1 true WO1992001383A1 (fr) 1992-02-06

Family

ID=3884881

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1991/000213 WO1992001383A1 (fr) 1990-07-23 1991-07-18 Procede de production de compositions contenant des produits pesticides biosynthetiques et utilisation des compositions ainsi obtenues

Country Status (10)

Country Link
EP (1) EP0499629A1 (fr)
JP (1) JPH05501723A (fr)
AU (1) AU8296491A (fr)
BE (1) BE1004492A3 (fr)
BR (1) BR9105844A (fr)
CA (1) CA2066664A1 (fr)
CS (1) CS85292A3 (fr)
HU (1) HUT65725A (fr)
PL (1) PL294568A1 (fr)
WO (1) WO1992001383A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009630A1 (fr) * 1992-11-05 1994-05-11 Novo Nordisk Entotech, Inc. POTENTIALISATEUR DE L'ACTIVITE PESTICIDE DE $i(BACILLUS)
US6268181B1 (en) 1992-11-05 2001-07-31 Valent Biosciences, Corporation Methods for producing a potentiator of Bacillus pesticidal activity
US6277624B1 (en) 1992-11-05 2001-08-21 Valent Biosciences, Inc. Mutants which produce a potentiator of Bacillus pesticidal activity
US6406691B1 (en) 1992-11-05 2002-06-18 Valent Biosciences Corp. Potentiator of Bacillus pesticidal activity

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000943A1 (fr) * 1980-09-15 1982-04-01 Battelle Development Corp Insecticide microbien

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000943A1 (fr) * 1980-09-15 1982-04-01 Battelle Development Corp Insecticide microbien

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Volume 101, No. 15, 8 October 1984, (Columbus, Ohio, US), H.S. SALAMA et al.: "Novel biochemical avenues for enhancing Bacillus thuringiensis endotoxin potency against Spodoptera littoralis", see page 201, Abstract 124514u, & Entomophaga 1984, 29(2), 171-178. *
J. Appl. Ent., Vol. 101, No. 3, 1986, H.S. SALAMA et al.: "Possible extension of the activity spectrum of Bacillus thuringiensis strains through chemical additives", see pages 304-313. *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009630A1 (fr) * 1992-11-05 1994-05-11 Novo Nordisk Entotech, Inc. POTENTIALISATEUR DE L'ACTIVITE PESTICIDE DE $i(BACILLUS)
AU683859B2 (en) * 1992-11-05 1997-11-27 Valent Biosciences Corporation Potentiator of (bacillus) pesticidal activity
US6268181B1 (en) 1992-11-05 2001-07-31 Valent Biosciences, Corporation Methods for producing a potentiator of Bacillus pesticidal activity
US6277624B1 (en) 1992-11-05 2001-08-21 Valent Biosciences, Inc. Mutants which produce a potentiator of Bacillus pesticidal activity
US6406691B1 (en) 1992-11-05 2002-06-18 Valent Biosciences Corp. Potentiator of Bacillus pesticidal activity
US6844182B2 (en) 1992-11-05 2005-01-18 Valent Biosciences Corporation Mutants which produce a potentiator of bacillus pesticidal activity

Also Published As

Publication number Publication date
BR9105844A (pt) 1992-09-22
CS85292A3 (en) 1992-08-12
BE1004492A3 (fr) 1992-12-01
CA2066664A1 (fr) 1992-01-24
AU8296491A (en) 1992-02-18
HU9201365D0 (en) 1992-07-28
JPH05501723A (ja) 1993-04-02
HUT65725A (en) 1994-07-28
PL294568A1 (en) 1992-11-30
EP0499629A1 (fr) 1992-08-26

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