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WO1992006188A3 - Polymerase d'adn thermostable - Google Patents

Polymerase d'adn thermostable Download PDF

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Publication number
WO1992006188A3
WO1992006188A3 PCT/US1991/007084 US9107084W WO9206188A3 WO 1992006188 A3 WO1992006188 A3 WO 1992006188A3 US 9107084 W US9107084 W US 9107084W WO 9206188 A3 WO9206188 A3 WO 9206188A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna polymerase
thermostable dna
taq dna
lacks
encodes
Prior art date
Application number
PCT/US1991/007084
Other languages
English (en)
Other versions
WO1992006188A2 (fr
Inventor
Wayne M Barnes
Original Assignee
Wayne M Barnes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wayne M Barnes filed Critical Wayne M Barnes
Priority to DE91920050T priority Critical patent/DE553264T1/de
Priority to JP3518348A priority patent/JPH06502303A/ja
Publication of WO1992006188A2 publication Critical patent/WO1992006188A2/fr
Publication of WO1992006188A3 publication Critical patent/WO1992006188A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Vecteur comprenant un acide nucléique codant une polymérase d'ADN ayant une séquence d'acides aminés identique à celle de la polymérase d'ADN de Thermus aquaticus appelée polymérase d'ADN Taq, excepté qu'il lui manque les 235 acides aminés N-terminaux de la polymérase d'ADN de Taq.
PCT/US1991/007084 1990-10-05 1991-09-27 Polymerase d'adn thermostable WO1992006188A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE91920050T DE553264T1 (de) 1990-10-05 1991-09-27 Thermostabile dna polymerase.
JP3518348A JPH06502303A (ja) 1990-10-05 1991-09-27 熱安定性ｄｎａポリメラーゼ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59463790A 1990-10-05 1990-10-05
US594,637 1990-10-05

Publications (2)

Publication Number Publication Date
WO1992006188A2 WO1992006188A2 (fr) 1992-04-16
WO1992006188A3 true WO1992006188A3 (fr) 1992-10-29

Family

ID=24379740

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/007084 WO1992006188A2 (fr) 1990-10-05 1991-09-27 Polymerase d'adn thermostable

Country Status (6)

Country Link
US (1) US5616494A (fr)
EP (1) EP0553264A4 (fr)
JP (1) JPH06502303A (fr)
AU (1) AU8906091A (fr)
DE (1) DE553264T1 (fr)
WO (1) WO1992006188A2 (fr)

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US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9358300B2 (en) 1998-11-12 2016-06-07 Life Technologies Corporation Transfection reagents
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US10064404B2 (en) 2014-06-10 2018-09-04 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US10568317B2 (en) 2015-12-08 2020-02-25 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate

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US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
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US9309520B2 (en) 2000-08-21 2016-04-12 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
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AU8906091A (en) 1992-04-28
EP0553264A4 (en) 1994-07-13
WO1992006188A2 (fr) 1992-04-16
US5616494A (en) 1997-04-01
DE553264T1 (de) 1994-04-28
JPH06502303A (ja) 1994-03-17
EP0553264A1 (fr) 1993-08-04

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