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WO1992014818A3 - Entomopoxvirus expression system comprising spheroidin or thymidine-kinase sequences - Google Patents

Entomopoxvirus expression system comprising spheroidin or thymidine-kinase sequences Download PDF

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Publication number
WO1992014818A3
WO1992014818A3 PCT/US1992/000855 US9200855W WO9214818A3 WO 1992014818 A3 WO1992014818 A3 WO 1992014818A3 US 9200855 W US9200855 W US 9200855W WO 9214818 A3 WO9214818 A3 WO 9214818A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequences
spheroidin
thymidine
expression system
entomopoxvirus
Prior art date
Application number
PCT/US1992/000855
Other languages
French (fr)
Other versions
WO1992014818A2 (en
Inventor
Richard W Moyer
Richard L Hall
Michael E Gruidl
Original Assignee
Univ Florida
Richard W Moyer
Richard L Hall
Michael E Gruidl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Richard W Moyer, Richard L Hall, Michael E Gruidl filed Critical Univ Florida
Priority to MX9200697A priority Critical patent/MX9200697A/en
Priority to JP4508743A priority patent/JPH06506594A/en
Priority to AU16634/92A priority patent/AU663709B2/en
Priority to IE051592A priority patent/IE920515A1/en
Priority to ZA921163A priority patent/ZA921163B/en
Priority to IL100983A priority patent/IL100983A0/en
Priority to CN92101985A priority patent/CN1065293A/en
Priority to NZ241662A priority patent/NZ241662A/en
Publication of WO1992014818A2 publication Critical patent/WO1992014818A2/en
Publication of WO1992014818A3 publication Critical patent/WO1992014818A3/en
Priority to US09/370,861 priority patent/US6410221B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C12N9/1211—Thymidine kinase (2.7.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.
PCT/US1992/000855 1991-02-19 1992-02-12 Entomopoxvirus expression system comprising spheroidin or thymidine-kinase sequences WO1992014818A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
MX9200697A MX9200697A (en) 1991-02-19 1992-02-12 ENTOMOPOXVIRUS EXPRESSION SYSTEM.
JP4508743A JPH06506594A (en) 1991-02-19 1992-02-12 Novel Entomovox virus expression system
AU16634/92A AU663709B2 (en) 1991-02-19 1992-02-12 Entomopoxvirus expression system comprising spheroidin or thymidine-kinase sequences
IE051592A IE920515A1 (en) 1991-02-19 1992-02-18 Novel Entomopoxvirus Expression System
ZA921163A ZA921163B (en) 1991-02-19 1992-02-18 Novel entomopoxvirus expression system
IL100983A IL100983A0 (en) 1991-02-19 1992-02-18 Entomopoxvirus expression system
CN92101985A CN1065293A (en) 1991-02-19 1992-02-19 New entomopoxvirus expression system
NZ241662A NZ241662A (en) 1991-02-19 1992-02-19 Entomopoxvirus spheroidin, thymidine kinase and fragments, recombinant polynucleotides
US09/370,861 US6410221B1 (en) 1991-02-19 1999-08-09 Entomopoxvirus expression system

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US65758491A 1991-02-19 1991-02-19
US657,584 1991-02-19
US82768592A 1992-01-30 1992-01-30
US827,685 1992-01-30

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US82768592A Continuation-In-Part 1991-02-19 1992-01-30

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US07/991,867 Continuation-In-Part US5476781A (en) 1991-02-19 1992-12-07 Entomopoxvirus spheroidin gene sequences

Publications (2)

Publication Number Publication Date
WO1992014818A2 WO1992014818A2 (en) 1992-09-03
WO1992014818A3 true WO1992014818A3 (en) 1992-12-10

Family

ID=27097441

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/000855 WO1992014818A2 (en) 1991-02-19 1992-02-12 Entomopoxvirus expression system comprising spheroidin or thymidine-kinase sequences

Country Status (12)

Country Link
EP (1) EP0573613A1 (en)
JP (1) JPH06506594A (en)
CN (1) CN1065293A (en)
AU (1) AU663709B2 (en)
CA (1) CA2103550A1 (en)
IE (1) IE920515A1 (en)
IL (1) IL100983A0 (en)
MX (1) MX9200697A (en)
NZ (1) NZ241662A (en)
WO (1) WO1992014818A2 (en)
YU (1) YU16292A (en)
ZA (1) ZA921163B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5476781A (en) * 1991-02-19 1995-12-19 University Of Florida Research Foundation, Inc. Entomopoxvirus spheroidin gene sequences
US6130074A (en) * 1992-06-01 2000-10-10 American Cyanamid Company Five Giralda Farms Recombinant insect virus with reduced capacity for host-to-host transmission in the environment and methods to produce said virus
JPH08501204A (en) * 1992-06-16 1996-02-13 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼーション Recombinant insect pox virus
US6106825A (en) * 1997-05-07 2000-08-22 University Of Florida Entomopoxvirus-vertebrate gene delivery vector and method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0397560A2 (en) * 1989-05-08 1990-11-14 Kai-Chung Leonard Yuen Spheroidin DNA isolate and recombinant entomopoxvirus expression vectors

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0397560A2 (en) * 1989-05-08 1990-11-14 Kai-Chung Leonard Yuen Spheroidin DNA isolate and recombinant entomopoxvirus expression vectors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Virology, vol. 175, no. 2, April 1990, Academic Press, Inc., L. Yuen et al.: "Identification and sequencing of the spheroidin gene of choristoneura biennis entomopoxvirus", pages 427-433, see the whole article *

Also Published As

Publication number Publication date
YU16292A (en) 1994-06-24
MX9200697A (en) 1993-03-01
IE920515A1 (en) 1992-08-26
JPH06506594A (en) 1994-07-28
NZ241662A (en) 1995-03-28
CA2103550A1 (en) 1992-08-20
ZA921163B (en) 1992-12-30
IL100983A0 (en) 1992-11-15
EP0573613A1 (en) 1993-12-15
WO1992014818A2 (en) 1992-09-03
AU1663492A (en) 1992-09-15
CN1065293A (en) 1992-10-14
AU663709B2 (en) 1995-10-19

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