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WO1993013797A3 - Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera - Google Patents

Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera Download PDF

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Publication number
WO1993013797A3
WO1993013797A3 PCT/US1993/000253 US9300253W WO9313797A3 WO 1993013797 A3 WO1993013797 A3 WO 1993013797A3 US 9300253 W US9300253 W US 9300253W WO 9313797 A3 WO9313797 A3 WO 9313797A3
Authority
WO
WIPO (PCT)
Prior art keywords
cholera
lps
conjugates
cholera toxin
disclosed
Prior art date
Application number
PCT/US1993/000253
Other languages
French (fr)
Other versions
WO1993013797B1 (en
WO1993013797A2 (en
Inventor
Shousun C Szu
John B Robbins
Rajesh K Gupta
Original Assignee
Us Health
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health filed Critical Us Health
Priority to AU34696/93A priority Critical patent/AU678549B2/en
Priority to JP5512624A priority patent/JPH07503238A/en
Priority to EP93903428A priority patent/EP0623026A1/en
Publication of WO1993013797A2 publication Critical patent/WO1993013797A2/en
Publication of WO1993013797A3 publication Critical patent/WO1993013797A3/en
Publication of WO1993013797B1 publication Critical patent/WO1993013797B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/107—Vibrio
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6087—Polysaccharides; Lipopolysaccharides [LPS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A vaccine formulation comprising conjugates of detoxified LPS with proteins including cholera toxin (CT) is disclosed. Treatment with hydrazine (DeA-LPS) reduces the endotoxic properties of the LPS to clinically acceptable levels and results in a larger and more antigenic molecule than the saccharide produced by acid hydrolysis. The conjugates utilizing the cholera toxin of V. cholerae are disclosed which have low levels of pyrogen, no toxic activity upon Chinese hamster overay cells and elicit booster responses of vibriocidal and CT antibodies when injected subcutaneously as saline solutions into mice. The conjugates produced as a cholera vaccine induce the same antibodies as parenterally injected cellular vaccines but have improved safety and immunologic properties.
PCT/US1993/000253 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera WO1993013797A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU34696/93A AU678549B2 (en) 1992-01-16 1993-01-14 Detoxified LPS-cholera toxin conjugate vaccine for prevention of cholera
JP5512624A JPH07503238A (en) 1992-01-16 1993-01-14 Detoxified LPS-cholera toxin conjugate vaccine for cholera prevention
EP93903428A EP0623026A1 (en) 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82145392A 1992-01-16 1992-01-16
US07/821,453 1992-01-16

Publications (3)

Publication Number Publication Date
WO1993013797A2 WO1993013797A2 (en) 1993-07-22
WO1993013797A3 true WO1993013797A3 (en) 1993-10-28
WO1993013797B1 WO1993013797B1 (en) 1994-01-20

Family

ID=25233450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/000253 WO1993013797A2 (en) 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera

Country Status (5)

Country Link
EP (1) EP0623026A1 (en)
JP (1) JPH07503238A (en)
AU (1) AU678549B2 (en)
CA (1) CA2128212A1 (en)
WO (1) WO1993013797A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998026799A1 (en) * 1996-12-18 1998-06-25 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Conjugate vaccine for salmonella paratyphi a
US6531131B1 (en) 1999-08-10 2003-03-11 The United States Of America As Represented By The Department Of Health And Human Services Conjugate vaccine for Neisseria meningitidis
JP2004515450A (en) 2000-04-18 2004-05-27 エンドバイオロジックス， インコーポレイテッド Lipopolysaccharide conjugate vaccine for the treatment of sepsis
US7749511B2 (en) 2000-04-18 2010-07-06 Endobiologics, Incorporated Anti-sepsis conjugate vaccine
WO2002020059A2 (en) * 2000-09-01 2002-03-14 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Vibrio cholerae o139 conjugate vaccines
WO2002080964A1 (en) * 2001-04-06 2002-10-17 Institut Pasteur Conjugate vaccine composed of the polysaccharide moiety of the lipopolysaccharide of vibrio cholerae 0139 bound to tetanus toxoid
WO2003094961A1 (en) 2002-05-09 2003-11-20 Massimo Porro Improved polysaccharide and glycoconjugate vaccines_____________
WO2004043489A1 (en) * 2002-11-14 2004-05-27 Instituto Finlay. Centro De Investigacion-Produccion De Vacunas Y Sueros. Method of obtaining conjugate vaccines and vaccine compositions containing same
US8048432B2 (en) 2003-08-06 2011-11-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Polysaccharide-protein conjugate vaccines
CN102824632A (en) * 2012-09-12 2012-12-19 北京民海生物科技有限公司 Polysaccharide conjugate vaccine of vibrio cholera group O1, preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
EP0306607A1 (en) * 1983-03-14 1989-03-15 Miles Inc. Vaccines for gram-negative bacteria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
EP0306607A1 (en) * 1983-03-14 1989-03-15 Miles Inc. Vaccines for gram-negative bacteria

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BROWN, F., R. M. CHANOCK AND R. A. LERNER (ED.). NEW APPROACHES TO IMMUNIZATION: DEVELOPING VACCINES AGAINST PARASITIC, BACTERIAL, AND VIRAL DISEASES; 1986, pages 231 - 234 KABIR, S. 'PREPARATION AND IMMUNOGENICITY OF A SEMISYNTHETIC BIVALENT CELL-SURFACE PROTEIN-POLYSACCHARIDE CONJUGATE OF VIBRIO-CHOLERAE.' *
EUR. J. BIOCHEM., VOL. 97, NO. 2, PAGE(S) 623-9, July 1979, TANAMOTO K ET AL. 'Regions of the lipopolysaccharide of Pseudomonas aeruginosa essential for antitumor and interferon-inducing activities.' *
INFECT. IMMUN., VOL. 13, NO. 3, PAGE(S) 735-740, 1976, WASHINGTON, US SVENNERHOLM A M ET AL. 'Synergistic protective effect in rabbits of immunization with vibrio cholerae lipopolysaccharide and toxin/toxoid.' *
INFECT. IMMUN., VOL. 60, NO. 8, PAGE(S) 3201-3208 1992, GUPTA R K ET AL 'Synthesis, characterization, and some immunological properties of conjugates composed of the detoxified lipopolysaccharide of Vibrio cholerae O1 serotype inaba bound to cholera toxin.' *
J. INFECT. DIS., VOL. 161, NO. , PAGE(S) 821-832, 1990, J. B. ROBBINS & R. SCHNEERSON 'Polysaccharide-protein conjugates: a new generation of vaccines.' *
MICROBIOL. ABSTR. B vol. 6, no. 1, October 1970, page 72 M. TORII ET AL. 'Immunochemical studies on O-antigens of Vibrio parahaemolyticus. 2. Effect of alkali and acid on specificity.' *
MICROBIOL. IMMUNOL., VOL. 23, NO. 2, PAGE(S) 87-94, 1979, EGAWA K ET AL. 'Endotoxic glycolipid as a potent depressor of the hepatic drug-metabolizing enzyme systems in mice.' *
PATENT ABSTRACTS OF JAPAN vol. 010, no. 287 (C-375)30 September 1986 *
SOUTHEAST ASIAN J TROP MED PUBLIC HEALTH., VOL. 18, NO. 2, PAGE(S) 142-148, 1987, CHAICUMPA W ET AL. 'ORAL VACCINE AGAINST CHOLERA PREPARED FROM VIBRIO-CHOLERAE ANTIGENS.' *
VACCINE, VOL. 9, NO. 1, PAGE(S) 53-59, January 1991, GB M. CHONGSA-NGUAN ET AL. 'IMMUNOGENECITY OF TWO FORMULATIONS OF ORAL CHOLERA VACCINES IN THAI VOLUNTEERS' *

Also Published As

Publication number Publication date
AU3469693A (en) 1993-08-03
JPH07503238A (en) 1995-04-06
WO1993013797A2 (en) 1993-07-22
EP0623026A1 (en) 1994-11-09
AU678549B2 (en) 1997-06-05
CA2128212A1 (en) 1993-07-22

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