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WO1996003511A3 - Procede de production d'acides gras ou derives a partir de plantes oleagineuses - Google Patents

Procede de production d'acides gras ou derives a partir de plantes oleagineuses Download PDF

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Publication number
WO1996003511A3
WO1996003511A3 PCT/FR1995/000957 FR9500957W WO9603511A3 WO 1996003511 A3 WO1996003511 A3 WO 1996003511A3 FR 9500957 W FR9500957 W FR 9500957W WO 9603511 A3 WO9603511 A3 WO 9603511A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
derivatives
lipase
oil plants
lipids
Prior art date
Application number
PCT/FR1995/000957
Other languages
English (en)
Other versions
WO1996003511A2 (fr
Inventor
Gilbert Alibert
Zephirin Mouloungui
Alain Boudet
Original Assignee
Toulouse Inst Nat Polytech
Gilbert Alibert
Zephirin Mouloungui
Alain Boudet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toulouse Inst Nat Polytech, Gilbert Alibert, Zephirin Mouloungui, Alain Boudet filed Critical Toulouse Inst Nat Polytech
Priority to US08/776,210 priority Critical patent/US5942659A/en
Priority to EP95925897A priority patent/EP0770134A2/fr
Priority to AU29849/95A priority patent/AU2984995A/en
Publication of WO1996003511A2 publication Critical patent/WO1996003511A2/fr
Publication of WO1996003511A3 publication Critical patent/WO1996003511A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

L'invention concerne un procédé de production d'acides gras ou de dérivés d'acides gras à partir de plantes oléagineuses. Ce procédé est caractérisé en ce qu'on produit des plantes oléagineuses transgéniques possédant, d'une part, au moins un gène codant pour une enzyme lipase, dit gène de lipase, d'autre part, associé à ce gène de lipase, un promoteur permettant une expression dudit gène soit dans des compartiments différents des compartiments d'accumulation des lipides, soit sur induction exogène, on recueille les graines ou fruits contenant les lipides des plantes, on les broie le cas échéant après traitement inducteur de façon à mettre en contact lipides et lipase, on laisse incuber l'ensemble pour engendrer une hydrolyse enzymatique des lipides et on extrait les acides gras ou dérivés.
PCT/FR1995/000957 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses WO1996003511A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US08/776,210 US5942659A (en) 1994-07-25 1995-07-18 Process for producing fatty acids or derivatives thereof from oleaginous plants
EP95925897A EP0770134A2 (fr) 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses
AU29849/95A AU2984995A (en) 1994-07-25 1995-07-18 Method for producing fatty acids or derivatives thereof from oil plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR94/09272 1994-07-25
FR9409272A FR2722798B1 (fr) 1994-07-25 1994-07-25 1procede de production d'acides gras2plantes oleagineuses

Publications (2)

Publication Number Publication Date
WO1996003511A2 WO1996003511A2 (fr) 1996-02-08
WO1996003511A3 true WO1996003511A3 (fr) 1996-04-25

Family

ID=9465782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1995/000957 WO1996003511A2 (fr) 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses

Country Status (6)

Country Link
US (1) US5942659A (fr)
EP (1) EP0770134A2 (fr)
AU (1) AU2984995A (fr)
CA (1) CA2195560A1 (fr)
FR (1) FR2722798B1 (fr)
WO (1) WO1996003511A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2733249B1 (fr) * 1995-04-20 1997-06-06 Biocem Lipase gastrique de chien recombinante et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations
FR2754827B1 (fr) * 1996-10-17 1998-12-24 Biocem Lipases pancreatiques et/ou colipases recombinantes et polypeptides dervies produits par les plantes, leurs procedes d'obtention et leurs utilisations
DE19731990A1 (de) * 1997-07-25 1999-01-28 Studiengesellschaft Kohle Mbh Verfahren zur Herstellung und Identifizierung von neuen Hydrolasen mit verbesserten Eigenschaften
US7166766B1 (en) 2000-04-03 2007-01-23 Total Raffinage Distribution S.A. Method for producing branched fatty acids using genetically modified plants
FR2769320B1 (fr) * 1997-10-03 2002-03-29 Total Raffinage Distrib Procede de production d'acides gras ramifies au moyen de plantes genetiquement modifiees
CA2362546C (fr) 1999-02-16 2012-05-15 Senesco, Inc. Adn codant pour une lipase vegetale, plantes transgeniques et methode de regulation de la senescence des plantes
GB0022236D0 (en) * 2000-09-11 2000-10-25 Pinet Aylette Partially hydrolysed protein nutrient supplement
EP1716230A2 (fr) 2004-02-20 2006-11-02 Pioneer Hi-Bred International, Inc. Lipases et procedes d'utilisation
WO2005083095A2 (fr) * 2004-02-20 2005-09-09 Pioneer Hi-Bred International, Inc. Methodes pour accroitre la resistance de vegetaux par rapport a des insectes
ES2713526T3 (es) * 2009-07-17 2019-05-22 Korea Advanced Inst Sci & Tech Procedimiento de producción de ésteres alquílicos de ácidos grasos usando microorganismos que tienen capacidad de producir aceite

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0427309A2 (fr) * 1989-11-09 1991-05-15 Unilever N.V. Procédé pour la préparation de graisses obtenues à partir de cruciferae
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1992005249A1 (fr) * 1990-09-13 1992-04-02 Novo Nordisk A/S Variantes lipasiques
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0427309A2 (fr) * 1989-11-09 1991-05-15 Unilever N.V. Procédé pour la préparation de graisses obtenues à partir de cruciferae
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1992005249A1 (fr) * 1990-09-13 1992-04-02 Novo Nordisk A/S Variantes lipasiques
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PEN, J., ET AL.: "PRODUCTION OF ACTIVE ALPHA-AMYLASE IN PLANTS AND THE APPLICATION IN STARCH LIQUEFACTION", JOURNAL OF CELLULAR BIOCHEMISTRY SUPPLEMENT, vol. 16F, pages 227 *
PEN, J., ET AL.: "PRODUCTION OF ACTIVE BACILLUS LICHENIFORMIS ALPHA-AMYLASEIN TOBACCO AND ITS APPLICATION IN STARCH LIQUEFACTION", BIOTECHNOLOGY, vol. 10, no. 3, NEW YORK US, pages 292 - 296 *

Also Published As

Publication number Publication date
WO1996003511A2 (fr) 1996-02-08
EP0770134A2 (fr) 1997-05-02
FR2722798B1 (fr) 1996-09-13
US5942659A (en) 1999-08-24
FR2722798A1 (fr) 1996-01-26
AU2984995A (en) 1996-02-22
CA2195560A1 (fr) 1996-02-08

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