[go: up one dir, main page]

WO1996008723A1 - Test rapide de differenciation des caracteristiques des erythrocytes - Google Patents

Test rapide de differenciation des caracteristiques des erythrocytes Download PDF

Info

Publication number
WO1996008723A1
WO1996008723A1 PCT/EP1995/003493 EP9503493W WO9608723A1 WO 1996008723 A1 WO1996008723 A1 WO 1996008723A1 EP 9503493 W EP9503493 W EP 9503493W WO 9608723 A1 WO9608723 A1 WO 9608723A1
Authority
WO
WIPO (PCT)
Prior art keywords
paper
rapid test
test according
zones
blood
Prior art date
Application number
PCT/EP1995/003493
Other languages
German (de)
English (en)
Inventor
Hans Seifert
Wolf Von Thun
Original Assignee
Nobis Labordiagnostica Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nobis Labordiagnostica Gmbh filed Critical Nobis Labordiagnostica Gmbh
Priority to EP95931995A priority Critical patent/EP0781415A1/fr
Priority to AU35222/95A priority patent/AU3522295A/en
Publication of WO1996008723A1 publication Critical patent/WO1996008723A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • G01N33/555—Red blood cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Definitions

  • the invention relates to a rapid test for differentiating erythrocyte characteristics, particularly suitable for use as a bedside test for the simple and quick determination of blood groups or their confirmation from capillary blood, but which can also be carried out with erythrocyte suspensions if the cell density corresponds to that of whole blood.
  • the test card can also be used to document the test.
  • Bedside tests are prescribed as confirmation tests immediately before the transfusion, since the donor is generally not present during the transfusion in order to avoid confusion of preserved blood. Such tests have been described since about 1908 and were first carried out in the presence of the donor. Since 1955, an ABO / Rh bedside test has been used on cardboard boxes with dried-in antisera, followed by tests with ready-to-use, liquid antisera, then dipsticks based on Elisa.
  • the test according to the invention is based on the method of capillary analysis or paper chromatography based on Runge and Schönbein.
  • the paper-chromatographic separation of proteins is a common but time-consuming method.
  • the determination of ABO group antigens in decayed blood using ascending paper chromatography in hydrochloric acid / methanol Systems described the antigens are determined after subsequent elution (Kuchkinov, A textbook. Vopr. Sud. Med. Ekspert. Veshchestv. Dokazatel'stv, 1982, 38-44 (Chemical Abstracts 101, 1984, 67173)).
  • This cumbersome procedure is of course completely unsuitable for a bedside test, in which speed, accuracy and documentability are important.
  • the object of the invention is therefore to provide a quick, easy-to-use, safe and documentable test for blood group confirmation.
  • capillary analytical blood group confirmation can be carried out using paper and a running medium, erythrocytes being transported through the running medium and permanent migration being available due to their different migration. If suitable antisera are added, this transport of the erythrocytes is blocked by their agglutination, since the agglutinates are immobile. Chromatography papers do not allow a differentiation between agglutinated and non-agglutinated bleeding, although the paper production for these papers has been optimized for consistency and reproducibility.
  • the invention therefore relates to a rapid test for differentiating erythrocyte features on paper, on which the corresponding antisera are applied, and the differentiation is possible optically and permanently through the different migration of the erythrocytes by means of an eluent.
  • Another object of the invention is therefore the use of the test according to the invention for the rapid determination of blood groups, their differentiation and / or the determination of blood group incompatibilities.
  • Another object of the invention is a so-called
  • Bedside test card for the quick and safe check of donor and recipient blood directly before the transfer and its documentation, as well as the production of these cards.
  • Suitable types of paper for the test according to the invention are unsized papers, preferably filter paper (such as laboratory filter paper or coffee filters), flow paper (such as kitchen paper, blotting paper, dry wipes made of paper or absorbent paper, for example for laboratory tables) or hygienic paper (such as toilet paper or household towels, towels, handkerchiefs) and paper napkins). Papers of the quality of toilet paper, wipes, coffee filters, absorbent paper are particularly preferred, paper qualities for coffee filters and absorbent paper for laboratory tables being very particularly preferred.
  • the papers can preferably also have a one-sided coating of solvent-impermeable material, preferably polyethylene, by means of which the attachment to the documentation test card is made considerably easier and which prevents solvents and test substances from penetrating into the test card.
  • FIG. 1 A practical embodiment of the rapid test according to the invention is shown as an example in FIG. 1.
  • the analysis field (3) has a comb-like appearance with several running zones (5), preferably four.
  • the width of the running zones (5) being able to be reduced to up to 0.5 mm and the distances between the running zones (5) being reduced to 0.1 mm.
  • the antisera applied in the antisera application zone (5) are mono- or polyclonal commercial preparations with the specificities anti-A, anti-B and anti-D, and monoclonal antisera are particularly preferred. They were found to be optimal for this procedure. At these positions, 2-5 ⁇ l antiserum in commercial concentration is applied manually as required and the blood group systems to be tested. For standard examinations, the antisera are preferably already applied industrially by customary printing processes.
  • the capillary blood (from the fingertip or the earlobe) or the donor blood is first placed on the storage field (8), which preferably consists of an impermeable and indifferent Foil exists from which the individual test fields are fed.
  • the remainder can preferably also be covered with a film after drying and serves as a reference sample for tests that may be required later, especially forensic tests.
  • 2-5 ⁇ l are then added to the test blood application zone (5).
  • a flow agent suitable for the transport of the erythrocytes such as, for example, LISS (lower ionic strength solution) or PBS (phosphate buffered saline) solutions, preferably physiological saline, is used as the flow agent for the analysis.
  • LISS lower ionic strength solution
  • PBS phosphate buffered saline
  • the superplasticizer front will reach the end of the running zone (7) after a few seconds, with the blood stain on agglutination at the application zone of the antisera (6 ) remains or moves with the superplasticizer if the agglutination is weak or missing.
  • the analysis field (3) shown in FIG. 1 is only to be regarded as an example within the scope of the invention and the restriction to 4 running zones (7) is only one possible embodiment. Embodiments with only one running zone and with any number are of course possible, so that a single test can be used to test for further antisera. Miniaturization is also possible, the width of the running zones (5) being able to be reduced to up to 0.5 mm and the distances between the running zones (5) being reduced to 0.1 mm. As a result, many can do so in a small space Different tests are done side by side with a very small amount of test blood.
  • a particular advantage of the test according to the invention is also the considerably reduced amount of test blood. Compared to 70 to 100 ⁇ l in previous tests, only 2 to 5 ⁇ l blood are required.
  • Monoclonal antisera of the specificities Anti-M and Anti-N, as well as Anti-Le-a and Anti-Le-b have also been tested in this way as rapid tests and offer the above-mentioned tests from capillary blood as well as from erythrocyte suspension. decisive time advantage in the Sehnell selection of blood supplies.
  • the test is therefore generally suitable for the rapid differentiation of erythrocyte characteristics with monoclonal antibodies of the IgM class, for example the Duffy, Kell-Cellano, Kidd, Lewis, Lutheran, MNSs, P , Rhesus, HLA system or the plasma protein polymorphism.
  • the rapid test according to the invention is particularly suitable for the industrial production of bedside test cards, in that a card made of paper, cardboard or a suitable plastic material is pre-printed with the necessary information and fields for receiving information. Then the analysis field (3) made of coated paper and the reference field (8) for taking the blood sample are glued on.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un test rapide de différenciation des caractéristiques des érythrocytes, dans lequel des antisérums déposés sur papier inhibent différemment la migration des érythrocytes provoquée par un solvant, ainsi que l'utilisation de ce test pour la détermination de groupes sanguins et de leurs compatibilités. L'invention concerne en outre une carte-test utilisable au chevet du malade, carte sur laquelle ce test est appliqué, ainsi que la fabrication de telles cartes.
PCT/EP1995/003493 1994-09-13 1995-09-06 Test rapide de differenciation des caracteristiques des erythrocytes WO1996008723A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP95931995A EP0781415A1 (fr) 1994-09-13 1995-09-06 Test rapide de differenciation des caracteristiques des erythrocytes
AU35222/95A AU3522295A (en) 1994-09-13 1995-09-06 Quick test for the differentiation of the features of erythrocytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944432485 DE4432485A1 (de) 1994-09-13 1994-09-13 Schnelltest zur Differenzierung von Erythrozytenmerkmalen
DEP4432485.5 1994-09-13

Publications (1)

Publication Number Publication Date
WO1996008723A1 true WO1996008723A1 (fr) 1996-03-21

Family

ID=6528042

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/003493 WO1996008723A1 (fr) 1994-09-13 1995-09-06 Test rapide de differenciation des caracteristiques des erythrocytes

Country Status (4)

Country Link
EP (1) EP0781415A1 (fr)
AU (1) AU3522295A (fr)
DE (1) DE4432485A1 (fr)
WO (1) WO1996008723A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10244154A1 (de) * 2002-09-23 2004-04-08 Prisma Diagnostika Gmbh Trägerelement für diagnostische Tests
US7145039B2 (en) 1998-11-12 2006-12-05 Invitrogen Corp. Transfection reagents
US10195280B2 (en) 2014-07-15 2019-02-05 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
CN117268877A (zh) * 2023-11-21 2023-12-22 军科正源（北京）药物研究有限责任公司 检测人泪液中的神经生长因子的方法及其泪液的处理方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A.T. KUCHKINOV: "Determination of ABO group antigens of purified blood by paper chromatography", AKTUAL VOPR SUD - MED EKSPERT VESHCHESTV DOKAZATEL'STV, TASHKENT USSR, pages 38 - 44 *
CHEMICAL ABSTRACTS, vol. 101, no. 9, Columbus, Ohio, US; abstract no. 67173f, page 173; *
M.E. REID ET AL.: "Structural relationships between human erythrocyte sialoglycoproteins beta and gamma and abnormal sialoglycoproteins found in certain rare human erythrocyte variants lacking the Gerbich blood-group antigen(s).", BIOCHEM J, vol. 244, no. 1, LONDON UK, pages 123 - 128 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7479573B2 (en) 1998-11-12 2009-01-20 Invitrogen Corporation Transfection reagents
US7601872B2 (en) 1998-11-12 2009-10-13 Life Technologies Corporation Transfection reagents
US7166745B1 (en) 1998-11-12 2007-01-23 Invitrogen Corporation Transfection reagents
US7173154B2 (en) 1998-11-12 2007-02-06 Invitrogen Corp. Transfection reagents
US7323594B2 (en) 1998-11-12 2008-01-29 Invitrogen Corporation Transfection reagents
US7470817B2 (en) 1998-11-12 2008-12-30 Invitrogen Corporation Transfection reagents
US9358300B2 (en) 1998-11-12 2016-06-07 Life Technologies Corporation Transfection reagents
US7145039B2 (en) 1998-11-12 2006-12-05 Invitrogen Corp. Transfection reagents
DE10244154A1 (de) * 2002-09-23 2004-04-08 Prisma Diagnostika Gmbh Trägerelement für diagnostische Tests
US11872285B2 (en) 2014-07-15 2024-01-16 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
US10792362B2 (en) 2014-07-15 2020-10-06 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
US10195280B2 (en) 2014-07-15 2019-02-05 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
CN117268877A (zh) * 2023-11-21 2023-12-22 军科正源（北京）药物研究有限责任公司 检测人泪液中的神经生长因子的方法及其泪液的处理方法
CN117268877B (zh) * 2023-11-21 2024-02-20 军科正源（北京）药物研究有限责任公司 检测人泪液中的神经生长因子的方法及其泪液的处理方法

Also Published As

Publication number Publication date
AU3522295A (en) 1996-03-29
EP0781415A1 (fr) 1997-07-02
DE4432485A1 (de) 1996-03-14

Similar Documents

Publication Publication Date Title
DE2723183C3 (de) Prüfmittel zur Bestimmung von Hämoglobin in einer Blutprobe
EP0415298B1 (fr) Procédé pour la détermination d'HDL-cholestérine utilisant un élément analytique avec fractionnement intégré
EP0989407B1 (fr) Méthode pour la détermination de l'hémoglobine glycosylée
DE2927345C2 (fr)
DE2618386C2 (de) Diagnostisches Verfahren zum Bestimmen der Anwesenheit oder Abwesenheit nachzuweisender biologischer Teilchen
DE3329728C2 (fr)
EP0353500B1 (fr) Bande-test pour la détermination analytique d'un composant dans un échantillon liquide
EP1143248B1 (fr) Méthode pour l'immobilisation de conjugates dans des épreuves diagnostiques
DE3887771T3 (de) Immunoassays und Vorrichtungen dafür.
DE69836493T2 (de) Vorrichtung zur bestimmung von mehreren analyten
DE3029579A1 (de) Mittel zur abtrennung von plasma oder serum aus vollblut
EP0133895A1 (fr) Substrat pour retenir les érythrocytes
DE9001870U1 (de) Analysentestvorrichtung
DE69005840T2 (de) Analytisches Element zur Untersuchung des gesamten Blutes.
DD143379A3 (de) Indikatorroehrchen zur glukosebestimmung
EP0811847A2 (fr) Méthode pour détecter des analytes sur une surface
DE1798423B2 (de) Analysierungsband fuer eine automatische analysierungseinrichtung
DE10330982A1 (de) Vorrichtung und Verfahren zur simultanen Bestimmung von Blutgruppenantigenen
DE3922960A1 (de) Verfahren zur bestimmung eines analyten
DE3784479T2 (de) Diagnostisches hilfsmittel, seine herstellung und seine verwendung.
DE3783316T2 (de) Verfahren zur bestimmung von bilirubin und ein dazu verwendbares element.
DE4439429A1 (de) Verfahren zum Nachweis der Kontamination einer Oberfläche mit einem Analyten
DE69819833T2 (de) Verfahren und Teststreife zur Veringerung des Harnstoffeinflusses bei immunochromatographischen Messungen unter Verwendung von Urinproben
WO1996008723A1 (fr) Test rapide de differenciation des caracteristiques des erythrocytes
DE69817793T2 (de) Detektion von hydrophoben analyten mit niedrigem gehalt in umweltproben

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AM AU BB BG BR BY CA CN CZ EE FI GE HU IS JP KG KP KR KZ LK LR LT LV MD MN MX NO NZ PL RO RU SG SI SK TJ TT UA US KE

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): KE MW SD SZ UG AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1995931995

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1995931995

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1995931995

Country of ref document: EP