WO1996017945B1 - Reversible nuclear genetic system for male sterility in transgenic plants - Google Patents
Reversible nuclear genetic system for male sterility in transgenic plantsInfo
- Publication number
- WO1996017945B1 WO1996017945B1 PCT/US1995/015229 US9515229W WO9617945B1 WO 1996017945 B1 WO1996017945 B1 WO 1996017945B1 US 9515229 W US9515229 W US 9515229W WO 9617945 B1 WO9617945 B1 WO 9617945B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- promoter
- plant
- anther
- dna sequence
- male sterility
- Prior art date
Links
- 206010021929 Infertility male Diseases 0.000 title claims abstract 3
- 208000007466 Male Infertility Diseases 0.000 title claims abstract 3
- 230000002068 genetic effect Effects 0.000 title abstract 3
- 230000002441 reversible effect Effects 0.000 title 1
- 230000009261 transgenic effect Effects 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract 6
- 230000015572 biosynthetic process Effects 0.000 claims abstract 3
- 101710112752 Cytotoxin Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 108010031746 Dam methyltransferase Proteins 0.000 claims 1
- 101000653753 Escherichia phage Mu Tail fiber protein S Proteins 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 231100000599 cytotoxic agent Toxicity 0.000 claims 1
- 239000002619 cytotoxin Substances 0.000 claims 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 230000008121 plant development Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000009877 rendering Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Abstract
Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.
Claims
AMENDED CLAIMS
[received by the International Bureau on 18 June 1996 (18.06.96); new claims 29 - 38 added; remaining claims unchanged (1 page)]
29. A method of producing a male sterile plant comprising the steps of : producing a transformed plant comprising a recombinant DNA molecule which comprises (i) a DNA sequence encoding a gene product which inhibits pollen formation or function and (ii) a promoter specific to cells critical to pollen formation or function operatively linked to said DNA sequence encoding said gene product; and growing said plant under conditions such that male sterility is achieved as a result of the expression of said DNA sequence .
30. The method of claim 29 wherein said gene product s a cytotoxin. 31. The method of claim 29 wherein said promoter is an anther-specific promoter.
32. The method of claim 31 wherein said anther- specific promoter is 5126 promoter.
33. The method of claim 29, wherein said DNA sequence encodes an exogenous methylase gene.
34. The method of claim 33, wherein said exogenous methylase gene is the DAM methylase gene.
35. The method of claim 34, wherein said promoter is an anther-specific promoter. 36. The method of claim 35, wherein said anther- specific promoter is 5126 promoter.
37. A plant produced by the method of claim 29.
38. A plant produced by the method of claim 1.
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU9701839A HU226920B1 (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
| CA002207264A CA2207264C (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
| KR1019970703834A KR100278819B1 (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
| JP51762796A JP3370677B2 (en) | 1994-12-08 | 1995-12-07 | A reversible nuclear genetic system for male sterility in transgenic plants |
| AU42436/96A AU698286B2 (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
| EP95940806A EP0797675B1 (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
| DE69535040T DE69535040T2 (en) | 1994-12-08 | 1995-12-07 | REVERSIBLE CORE GENETIC SYSTEM FOR MALE STERILITY IN TRANSGENIC PLANTS |
| MX9704270A MX9704270A (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants. |
| NZ297303A NZ297303A (en) | 1994-12-08 | 1995-12-07 | Producing reversible male sterility in plants using a reversible nuclear genetic system |
| BR9510456A BR9510456A (en) | 1994-12-08 | 1995-12-07 | Process for the production of reversible male sterility in a plant |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/351,899 US5750868A (en) | 1994-12-08 | 1994-12-08 | Reversible nuclear genetic system for male sterility in transgenic plants |
| US08/351,899 | 1994-12-08 | ||
| US08/474,556 | 1995-06-07 | ||
| US08/474,556 US5689051A (en) | 1994-12-08 | 1995-06-07 | Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996017945A1 WO1996017945A1 (en) | 1996-06-13 |
| WO1996017945B1 true WO1996017945B1 (en) | 1996-07-25 |
Family
ID=26997302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1995/015229 WO1996017945A1 (en) | 1994-12-08 | 1995-12-07 | Reversible nuclear genetic system for male sterility in transgenic plants |
Country Status (13)
| Country | Link |
|---|---|
| US (5) | US5763243A (en) |
| EP (3) | EP1731614A1 (en) |
| JP (2) | JP3370677B2 (en) |
| AR (1) | AR000291A1 (en) |
| AT (1) | ATE329040T1 (en) |
| AU (1) | AU698286B2 (en) |
| DE (1) | DE69535040T2 (en) |
| ES (1) | ES2267105T3 (en) |
| HU (2) | HU226920B1 (en) |
| MX (1) | MX9704270A (en) |
| NZ (1) | NZ297303A (en) |
| PT (1) | PT797675E (en) |
| WO (1) | WO1996017945A1 (en) |
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| AR075466A1 (en) | 2008-10-22 | 2011-04-06 | Basf Se | USE OF AUXINE TYPE HERBICIDES IN CULTIVATED PLANTS |
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| BR112013033176A2 (en) | 2011-06-21 | 2018-06-12 | E I Du Pont De Nemouras And Company | method for producing a targeted modification on a male fertility gene, plant, isolated nucleic acid molecule, expression construct |
| WO2013138309A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
| WO2013138358A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
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| CN105579583B (en) | 2014-01-02 | 2017-12-12 | 北京思创达科技有限公司 | Reproduction of Female Sterile Lines and Hybrid Seed Production Techniques |
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-
1995
- 1995-06-07 US US08/479,382 patent/US5763243A/en not_active Expired - Lifetime
- 1995-06-07 US US08/479,041 patent/US5837851A/en not_active Expired - Lifetime
- 1995-06-07 US US08/479,383 patent/US5795753A/en not_active Expired - Lifetime
- 1995-12-06 AR AR33452095A patent/AR000291A1/en unknown
- 1995-12-07 MX MX9704270A patent/MX9704270A/en unknown
- 1995-12-07 AT AT95940806T patent/ATE329040T1/en not_active IP Right Cessation
- 1995-12-07 PT PT95940806T patent/PT797675E/en unknown
- 1995-12-07 AU AU42436/96A patent/AU698286B2/en not_active Expired
- 1995-12-07 EP EP06011502A patent/EP1731614A1/en not_active Withdrawn
- 1995-12-07 JP JP51762796A patent/JP3370677B2/en not_active Expired - Lifetime
- 1995-12-07 ES ES95940806T patent/ES2267105T3/en not_active Expired - Lifetime
- 1995-12-07 HU HU9701839A patent/HU226920B1/en not_active IP Right Cessation
- 1995-12-07 WO PCT/US1995/015229 patent/WO1996017945A1/en active IP Right Grant
- 1995-12-07 EP EP95940806A patent/EP0797675B1/en not_active Expired - Lifetime
- 1995-12-07 NZ NZ297303A patent/NZ297303A/en not_active IP Right Cessation
- 1995-12-07 EP EP02016942A patent/EP1273662B1/en not_active Expired - Lifetime
- 1995-12-07 HU HU0301904A patent/HU227303B1/en not_active IP Right Cessation
- 1995-12-07 DE DE69535040T patent/DE69535040T2/en not_active Expired - Lifetime
-
1997
- 1997-03-17 US US08/819,646 patent/US6281348B1/en not_active Expired - Lifetime
-
1998
- 1998-11-19 US US09/195,716 patent/US6399856B1/en not_active Expired - Lifetime
-
2002
- 2002-03-11 JP JP2002066072A patent/JP2002354954A/en active Pending
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