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WO1996019587A3 - Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports - Google Patents

Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports Download PDF

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Publication number
WO1996019587A3
WO1996019587A3 PCT/US1995/016627 US9516627W WO9619587A3 WO 1996019587 A3 WO1996019587 A3 WO 1996019587A3 US 9516627 W US9516627 W US 9516627W WO 9619587 A3 WO9619587 A3 WO 9619587A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
methods
immobilizing
solid support
support material
Prior art date
Application number
PCT/US1995/016627
Other languages
English (en)
Other versions
WO1996019587A2 (fr
Inventor
Tsung-Hui K Jou
Joanell V Hoijer
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Priority to EP95943480A priority Critical patent/EP0795032A2/fr
Publication of WO1996019587A2 publication Critical patent/WO1996019587A2/fr
Publication of WO1996019587A3 publication Critical patent/WO1996019587A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé d'immobilisation directe d'un oligonucléotide sur un matériau support consistant à mettre en contact une solution d'oligonucléotides avec un matériau support solide puis à dessécher ladite solution sur le support. Les oligonucléotides et leur support d'immobilisation peuvent servir de réactifs de capture pour l'immobilisation des séquences nucléotidiques complémentaires des oligonucléotides immobilisés. On maintient de cette façon la capacité d'hybridation des oligonucléotides directement immobilisés. Sont également présentés des procédés de détermination de la présence ou du volume de séquences nucléotidiques dans un échantillon d'essai.
PCT/US1995/016627 1994-12-22 1995-12-20 Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports WO1996019587A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP95943480A EP0795032A2 (fr) 1994-12-22 1995-12-20 Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36228194A 1994-12-22 1994-12-22
US08/362,281 1994-12-22

Publications (2)

Publication Number Publication Date
WO1996019587A2 WO1996019587A2 (fr) 1996-06-27
WO1996019587A3 true WO1996019587A3 (fr) 1996-09-19

Family

ID=23425472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/016627 WO1996019587A2 (fr) 1994-12-22 1995-12-20 Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports

Country Status (3)

Country Link
EP (1) EP0795032A2 (fr)
CA (1) CA2207629A1 (fr)
WO (1) WO1996019587A2 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991903B2 (en) 1992-11-06 2006-01-31 Sequenom, Inc. Solid phase sequencing of double-stranded nucleic acids
US7198893B1 (en) 1996-11-06 2007-04-03 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7285422B1 (en) 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
US7319003B2 (en) 1992-11-06 2008-01-15 The Trustees Of Boston University Arrays of probes for positional sequencing by hybridization
USRE41005E1 (en) 1996-11-06 2009-11-24 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US8999266B2 (en) 2000-10-30 2015-04-07 Agena Bioscience, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638760B1 (en) * 1998-11-25 2003-10-28 Pe Corporation (Ny) Method and apparatus for flow-through hybridization
KR100459865B1 (ko) * 2001-11-26 2004-12-03 아람 바이오시스템 주식회사 유전적 염기서열 고정 방법
US8314226B2 (en) 2004-03-29 2012-11-20 The General Hospital Corporation Oligonucleotide complex compositions and methods of use as gene alteration tools
WO2009039122A2 (fr) 2007-09-17 2009-03-26 Sequenom, Inc. Dispositif de transfert d'échantillon robotique intégré

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3708513A1 (de) * 1986-03-21 1987-09-24 Block Myron J Verfahren und vorrichtung zur nukleinsaeure-analyse
EP0320842A2 (fr) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Méthode pour attacher les réactifs biologiques à des supports poreux
WO1990006503A2 (fr) * 1988-11-29 1990-06-14 Applied Research Systems Ars Holding N.V. Capteur pour analyse optique
WO1992020702A1 (fr) * 1991-05-24 1992-11-26 Ole Buchardt Acides nucleiques de peptides
EP0524864A1 (fr) * 1991-07-17 1993-01-27 BIO MERIEUX, Société anonyme Procédé d'immobilisation d'un fragment d'acide nucléique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation
WO1993006241A1 (fr) * 1991-09-16 1993-04-01 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Procede de capteur biologique de sonde genique
WO1993020240A1 (fr) * 1992-04-06 1993-10-14 Abbott Laboratories Procede et dispositif de detection d'acide nucleique ou d'analyte au moyen d'une technique de reflexion interne totale
EP0605828A1 (fr) * 1993-01-04 1994-07-13 Becton, Dickinson and Company Essai d'hybridation à écoulement de séquences oligonucléotidiques
EP0614987A1 (fr) * 1993-02-26 1994-09-14 Becton, Dickinson and Company Test d'hybridation d'acide nucléique utilisant un écoulement à travers une membrane
WO1996009532A1 (fr) * 1994-09-22 1996-03-28 Abbott Laboratories Procede utilisant un guide d'ondes optique pour detecter des evenements de liaison specifique par diffusion de la lumiere

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3708513A1 (de) * 1986-03-21 1987-09-24 Block Myron J Verfahren und vorrichtung zur nukleinsaeure-analyse
EP0320842A2 (fr) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Méthode pour attacher les réactifs biologiques à des supports poreux
WO1990006503A2 (fr) * 1988-11-29 1990-06-14 Applied Research Systems Ars Holding N.V. Capteur pour analyse optique
WO1992020702A1 (fr) * 1991-05-24 1992-11-26 Ole Buchardt Acides nucleiques de peptides
EP0524864A1 (fr) * 1991-07-17 1993-01-27 BIO MERIEUX, Société anonyme Procédé d'immobilisation d'un fragment d'acide nucléique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation
WO1993006241A1 (fr) * 1991-09-16 1993-04-01 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Procede de capteur biologique de sonde genique
WO1993020240A1 (fr) * 1992-04-06 1993-10-14 Abbott Laboratories Procede et dispositif de detection d'acide nucleique ou d'analyte au moyen d'une technique de reflexion interne totale
EP0605828A1 (fr) * 1993-01-04 1994-07-13 Becton, Dickinson and Company Essai d'hybridation à écoulement de séquences oligonucléotidiques
EP0614987A1 (fr) * 1993-02-26 1994-09-14 Becton, Dickinson and Company Test d'hybridation d'acide nucléique utilisant un écoulement à travers une membrane
WO1996009532A1 (fr) * 1994-09-22 1996-03-28 Abbott Laboratories Procede utilisant un guide d'ondes optique pour detecter des evenements de liaison specifique par diffusion de la lumiere

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ELAISSARI A ET AL: "ADSORPTION OF OLIGONUCLEOTIDES ONTO NEGATIVELY AND POSITIVELY CHARGED LATEX-PARTICLES", COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1994, 83, 25-31, XP002009485 *
F.M.AUSUBEL ET AL.: "Current protocols in molecular biology", 1993, JHON WILEY & SONS, INC., XP002009489 *
NIERZWICKI-BAUER S.A. ET AL.: "A comparison of UV cross-linking and vacuum baking for nucleic acid immobilization and retention", BIOTECHNIQUES, vol. 9, no. 4, 1990, pages 472 - 478, XP002009486 *
SAMBROOK J. ET AL.: "Molecular cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS, XP002009488 *
ZENDEGUI, JOSEPH G. ET AL: "In vivo stability and kinetics of absorption and disposition of 3' phosphopropyl amine oligonucleotides", NUCLEIC ACIDS RES. (1992), 20(2), 307-14 CODEN: NARHAD;ISSN: 0305-1048, XP002009487 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991903B2 (en) 1992-11-06 2006-01-31 Sequenom, Inc. Solid phase sequencing of double-stranded nucleic acids
US7319003B2 (en) 1992-11-06 2008-01-15 The Trustees Of Boston University Arrays of probes for positional sequencing by hybridization
US7198893B1 (en) 1996-11-06 2007-04-03 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7501251B2 (en) 1996-11-06 2009-03-10 Sequenom, Inc. DNA diagnostics based on mass spectrometry
USRE41005E1 (en) 1996-11-06 2009-11-24 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
USRE44693E1 (en) 1996-11-06 2014-01-07 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US7285422B1 (en) 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
US8999266B2 (en) 2000-10-30 2015-04-07 Agena Bioscience, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate

Also Published As

Publication number Publication date
CA2207629A1 (fr) 1996-06-27
WO1996019587A2 (fr) 1996-06-27
EP0795032A2 (fr) 1997-09-17

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