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WO1996036881A3 - Screening for inhibitors of tcr-mhc interactions - Google Patents

Screening for inhibitors of tcr-mhc interactions Download PDF

Info

Publication number
WO1996036881A3
WO1996036881A3 PCT/GB1996/001165 GB9601165W WO9636881A3 WO 1996036881 A3 WO1996036881 A3 WO 1996036881A3 GB 9601165 W GB9601165 W GB 9601165W WO 9636881 A3 WO9636881 A3 WO 9636881A3
Authority
WO
WIPO (PCT)
Prior art keywords
tcr
mhc
peptide ligand
responder cells
signal produced
Prior art date
Application number
PCT/GB1996/001165
Other languages
French (fr)
Other versions
WO1996036881A2 (en
Inventor
Dan Hung Barouch
Stephen John Rupert Vessey
Bent Karsten Jakobsen
Original Assignee
Cancer Res Campaign Tech
Dan Hung Barouch
Stephen John Rupert Vessey
Bent Karsten Jakobsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cancer Res Campaign Tech, Dan Hung Barouch, Stephen John Rupert Vessey, Bent Karsten Jakobsen filed Critical Cancer Res Campaign Tech
Priority to AU57690/96A priority Critical patent/AU5769096A/en
Publication of WO1996036881A2 publication Critical patent/WO1996036881A2/en
Publication of WO1996036881A3 publication Critical patent/WO1996036881A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates to the interactions between T-cell receptors and their specific MHC-peptide ligand. A screening method is provided to identify substances which inhibit the specific interaction between a T-cell receptor (TCR) and an MHC-peptide ligand involving incubating responder cells expressing a TCR with (i) an MHC-peptide ligand which stimulates said responder cells; and (ii) a candidate inhibitory substance; and monitoring a signal produced by said responder cells when the TCR engages the MHC-peptide ligand; and comparing said signal produced by said responder cells on the engagement of TCR with the MHC-peptide ligand with a control signal produced by said responder cells when incubated with (i) but not (ii); wherein a reduction in signal associated with the presence of (ii) in the incubation represents the efficacy of the inhibitor.
PCT/GB1996/001165 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions WO1996036881A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU57690/96A AU5769096A (en) 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9509844.8A GB9509844D0 (en) 1995-05-16 1995-05-16 Screening for inhibitors of TCR-MHC interactions
GB9509844.8 1995-05-16

Publications (2)

Publication Number Publication Date
WO1996036881A2 WO1996036881A2 (en) 1996-11-21
WO1996036881A3 true WO1996036881A3 (en) 1997-01-09

Family

ID=10774510

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1996/001165 WO1996036881A2 (en) 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions

Country Status (3)

Country Link
AU (1) AU5769096A (en)
GB (1) GB9509844D0 (en)
WO (1) WO1996036881A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6309820B1 (en) 1995-04-07 2001-10-30 University Of North Carolina At Chapel Hill Polypeptides having a functional domain of interest and methods of identifying and using same
EP0833941B1 (en) * 1995-04-07 2008-10-22 Cytogen Corporation Polypeptides having a functional domain of interest and methods of identifying and using same
WO1997044667A2 (en) * 1996-05-21 1997-11-27 Institut Pasteur Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells
WO1999038526A1 (en) * 1998-01-29 1999-08-05 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Variant peptide ligands that selectively induce apoptosis
CA2410642A1 (en) * 2000-05-25 2001-11-29 Sunol Molecular Corporation Modulation of t-cell receptor interactions
WO2020028595A2 (en) * 2018-07-31 2020-02-06 Janux Therapeutics, Inc. Inhibitory t cell receptor peptides and discovery methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016234A1 (en) * 1991-03-22 1992-10-01 The Trustees Of The University Of Pennsylvania Method of modulating mammalian t-cell response
WO1995007707A1 (en) * 1993-09-14 1995-03-23 Cytel Corporation Alteration of immune response using pan dr-binding peptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016234A1 (en) * 1991-03-22 1992-10-01 The Trustees Of The University Of Pennsylvania Method of modulating mammalian t-cell response
WO1995007707A1 (en) * 1993-09-14 1995-03-23 Cytel Corporation Alteration of immune response using pan dr-binding peptides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J. MADRENAS ET AL.: "Zeta phosphorylation without ZAP-70 activation induced by TcR antagonists or partial agonists.", SCIENCE, vol. 267, no. 5197, 27 January 1995 (1995-01-27), WASHINGTON, DC, USA, pages 515 - 518, XP002015729 *
S. JAMESON ET AL.: "Clone-specific T cell receptor antagonists of major histocompatibility complex class I-restricted cytotoxic T cells.", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 177, no. 6, 1 June 1993 (1993-06-01), NEW YORK, NY, USA, pages 1541 - 1550, XP000604978 *
S. WEBER ET AL.: "Specific low-affinity recognition of major histocompatibility complex plus peptide by soluble T-cell receptor.", NATURE, vol. 356, 30 April 1992 (1992-04-30), LONDON, GB, pages 793 - 796, XP002008364 *
W. WILLIAMS ET AL.: "Modulation of T cell responses with MHC-derived peptides.", IMMUNOLOGICAL RESEARCH, vol. 11, no. 1, March 1992 (1992-03-01), BASEL, SWITZERLAND, pages 11 - 23, XP002015728 *

Also Published As

Publication number Publication date
AU5769096A (en) 1996-11-29
WO1996036881A2 (en) 1996-11-21
GB9509844D0 (en) 1995-07-12

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