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WO1997006268A3 - Constructions d'adn - Google Patents

Constructions d'adn Download PDF

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Publication number
WO1997006268A3
WO1997006268A3 PCT/GB1996/001846 GB9601846W WO9706268A3 WO 1997006268 A3 WO1997006268 A3 WO 1997006268A3 GB 9601846 W GB9601846 W GB 9601846W WO 9706268 A3 WO9706268 A3 WO 9706268A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
gene
insects
encodes
damaging
Prior art date
Application number
PCT/GB1996/001846
Other languages
English (en)
Other versions
WO1997006268A2 (fr
Inventor
Ian Jepson
Jacqueline Ann Mary Paine
Original Assignee
Zeneca Ltd
Ian Jepson
Jacqueline Ann Mary Paine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Ian Jepson, Jacqueline Ann Mary Paine filed Critical Zeneca Ltd
Priority to CA002227445A priority Critical patent/CA2227445A1/fr
Priority to JP9508208A priority patent/JPH11510694A/ja
Priority to BR9609873A priority patent/BR9609873A/pt
Priority to NZ313724A priority patent/NZ313724A/en
Priority to EP96925889A priority patent/EP0846179A2/fr
Priority to PL96324880A priority patent/PL324880A1/xx
Priority to MX9801008A priority patent/MX9801008A/es
Priority to APAP/P/1998/001194A priority patent/AP863A/en
Priority to AU66252/96A priority patent/AU704172B2/en
Priority to IL12317296A priority patent/IL123172A0/xx
Priority to SK169-98A priority patent/SK16998A3/sk
Publication of WO1997006268A2 publication Critical patent/WO1997006268A2/fr
Publication of WO1997006268A3 publication Critical patent/WO1997006268A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On décrit une cassette d'expression de gènes de plantes pouvant être induite chimiquement, qui comprend un premier promoteur lié de manière fonctionnelle à une séquence de régulation, laquelle est dérivée du gène alcR et code une protéine régulatrice, ainsi qu'un promoteur pouvant être induit et qui est lié de manière fonctionnelle à un gène cible codant une protéine, laquelle est préjudiciable aux insectes, ou dont l'expression induit un processus métabolique produisant un métabolite, lequel est préjudiciable aux insectes. Le promoteur pouvant être induit est activé par la protéine régulatrice en présence d'un inducteur exogène efficace, une application de cet inducteur provoquant l'expression du gène cible.
PCT/GB1996/001846 1995-08-08 1996-07-29 Constructions d'adn WO1997006268A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CA002227445A CA2227445A1 (fr) 1995-08-08 1996-07-29 Constructions d'adn
JP9508208A JPH11510694A (ja) 1995-08-08 1996-07-29 Ｄｎａ構築体
BR9609873A BR9609873A (pt) 1995-08-08 1996-07-29 Cassete de expressão de gene de planta quimicamente indutível célula de planta tecido de planta planta semente e processo par controlar insetos
NZ313724A NZ313724A (en) 1995-08-08 1996-07-29 Dna constructs and promoter sequences and their use in the expression of genes which confer insecticidal activity on plants
EP96925889A EP0846179A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn
PL96324880A PL324880A1 (en) 1995-08-08 1996-07-29 Dna constructs
MX9801008A MX9801008A (es) 1995-08-08 1996-07-29 Constructos de adn.
APAP/P/1998/001194A AP863A (en) 1995-08-08 1996-07-29 Dna constructs.
AU66252/96A AU704172B2 (en) 1995-08-08 1996-07-29 DNA constructs
IL12317296A IL123172A0 (en) 1995-08-08 1996-07-29 Dna constructs
SK169-98A SK16998A3 (en) 1995-08-08 1996-07-29 Chemically-inducible plant gene expression cassette

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9516241.8A GB9516241D0 (en) 1995-08-08 1995-08-08 Dna constructs
GB9516241.8 1995-08-08

Publications (2)

Publication Number Publication Date
WO1997006268A2 WO1997006268A2 (fr) 1997-02-20
WO1997006268A3 true WO1997006268A3 (fr) 1997-03-13

Family

ID=10778942

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1996/001846 WO1997006268A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn

Country Status (19)

Country Link
EP (1) EP0846179A2 (fr)
JP (1) JPH11510694A (fr)
KR (1) KR19990036251A (fr)
CN (1) CN1199425A (fr)
AP (1) AP863A (fr)
AR (1) AR002914A1 (fr)
AU (1) AU704172B2 (fr)
BR (1) BR9609873A (fr)
CA (1) CA2227445A1 (fr)
CZ (1) CZ36998A3 (fr)
GB (1) GB9516241D0 (fr)
HU (1) HUP9900057A3 (fr)
IL (1) IL123172A0 (fr)
MX (1) MX9801008A (fr)
NZ (1) NZ313724A (fr)
PL (1) PL324880A1 (fr)
SK (1) SK16998A3 (fr)
TR (1) TR199800177T1 (fr)
WO (1) WO1997006268A2 (fr)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
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GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
GB9817704D0 (en) * 1998-08-13 1998-10-07 Zeneca Ltd Gene switch
GB9902236D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Formulation
GB9902234D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Expression system
GB9918154D0 (en) * 1999-08-02 1999-10-06 Zeneca Ltd Expression system
EP1925672A1 (fr) 2001-06-22 2008-05-28 Syngeta Participations AG Polynucléotides et polypeptides sensibles au stress abiotique
DE10212158A1 (de) 2002-03-19 2003-10-02 Metanomics Gmbh & Co Kgaa Population transgener Pflanzen, davon abgeleitetes biologisches Material, entsprechende Plasmidkollektion und Population transformierter Wirtsorganismen, sowie deren Verwendung und Verfahren zu deren Erzeugung
AU2003294924A1 (en) 2002-12-20 2004-07-14 Metanomics Gmbh & Co. Kgaa Method for producing aminoacids
WO2004074440A2 (fr) 2003-02-17 2004-09-02 Metanomics Gmbh Preparation d'organismes a croissance plus rapide et/ou a rendement plus eleve
AU2004262656A1 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
CA2559760A1 (fr) 2004-07-02 2006-07-06 Metanomics Gmbh Procede de production de produits chimiques fins
WO2006013010A2 (fr) 2004-07-31 2006-02-09 Metanomics Gmbh Preparation d'organismes a croissance plus rapide et/ou a meilleur rendement
MX2007007040A (es) 2004-12-17 2008-10-24 Metanomics Gmbh Proceso para el control de produccion de productos quimicos finos.
EP2573188A2 (fr) 2005-03-02 2013-03-27 Metanomics GmbH Procédé de production de produits chimiques fins
EP1871883A1 (fr) 2005-03-02 2008-01-02 Metanomics GmbH Procede de production de produits chimiques fins
EP1859037A2 (fr) 2005-03-08 2007-11-28 BASF Plant Science GmbH Sequences introniques facilitant l'expression
WO2007003660A1 (fr) 2005-07-06 2007-01-11 Cropdesign N.V. Amelioration du rendement de plantes par expression de gene de la famille ste20
AR055439A1 (es) 2005-09-15 2007-08-22 Cropdesign Nv Plantas con rendimiento aumentado y metodo para elaborarlas
WO2007113237A2 (fr) 2006-03-31 2007-10-11 Basf Plant Science Gmbh Plantes ayant des caractéristiques de rendement de production accrues et leur procédé de fabrication
AU2007299219A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
BRPI0713097A2 (pt) 2006-05-31 2012-10-16 Metanomics Gmbh processo para a assimilação, acúmulo e/ou utilização de nitrogênio intensificados e/ou para o teor de nitrogênio total aumentado em um organismo fotossintético ativo, molécula de ácido nucleico isolada, construção de ácido nucleico, vetor, célula hospedeira, processo para produzir um polipeptìdeo, polipeptìdeo, anticorpo, tecido de planta, material de propagação, material colhido ou uma planta, método para a identificação de um produto de gene, composição, e, uso da molécula de ácido nucleico, do polipeptìdeo, da construção de ácido nucleico, do vetor, da planta ou tecido de planta, do material colhido da célula hospedeira ou do produto de gene identificado de acordo com o método
EP2436761A1 (fr) 2006-06-08 2012-04-04 BASF Plant Science GmbH Installations dotées de caractéristiques de croissance améliorées et procédé de fabrication de celles-ci
EP2199394A1 (fr) 2006-08-02 2010-06-23 CropDesign N.V. Plantes transformées avec un polypeptide-syt qui sous un stress abiotique, presentent un rendement amelioré, et leur procédé de production
ITUD20060280A1 (it) 2006-12-29 2008-06-30 Univ Degli Studi Udine Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta
EP2492345A3 (fr) 2007-05-04 2012-12-12 BASF Plant Science GmbH Amélioration de semences par des combinaisons de pyruvate kinase
BRPI0811843A2 (pt) 2007-05-22 2014-10-07 Basf Plant Science Gmbh Métodos para a produção de uma planta transgênica, e para seleção de antagonistas, molécula, iniciador, mutante dominante negativo de um polipeptídeo, construto de ácido nucleico, vetor, célula de planta transgênica, planta ou uma parte da mesma, polipeptídeo isolado, anticorpo, tecido de planta, planta, material de planta colhido ou material de propagação de uma planta, processos para a produção de um polipeptídeo, e para a identificação de um composto, composição, e, uso da molécula de ácido nucleico, do construto de ácido nucleico e do vetor
BRPI0820653B1 (pt) 2007-12-03 2023-05-16 Syngenta Participations Ag Fitase isolada termotolerante e enzimaticamente suscetível, polinucleotídeo, cassete de expressão, vetor, célula hospedeira microbiana, método para preparar a referida fitase, preparação e ração animal
CN101952444A (zh) 2007-12-21 2011-01-19 巴斯夫植物科学有限公司 产量提高的植物(ko nue)
US20130055471A1 (en) 2009-12-15 2013-02-28 Edwin Henricus Antonius HOLMAN Transgenic Ozone-Resistant Plants
AR083029A1 (es) 2010-12-09 2013-01-23 Syngenta Participations Ag Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
AU2011355690A1 (en) 2011-01-18 2013-07-11 Queensland University Of Technology Methods and compositions for modified ethanol inducible promoter systems
WO2013059507A1 (fr) 2011-10-20 2013-04-25 University Of Iowa Research Foundation Gènesn-déméthylase et leurs utilisations
AU2013219927B2 (en) 2012-02-16 2018-11-08 Syngenta Participations Ag Engineered pesticidal proteins
EP2861740A1 (fr) 2012-06-15 2015-04-22 University of Iowa Research Foundation Promoteurs sensibles à la caféine et gènes régulateurs
CA2890235A1 (fr) 2012-06-22 2013-12-27 Syngenta Participations Ag Lutte biologique contre les coleopteres nuisibles
EP2895610B1 (fr) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Compositions et systèmes pour conférer une résistance à une maladie à des plantes et leurs procédés d'utilisation
AU2015248815B2 (en) 2014-04-17 2021-07-08 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
CA2945877C (fr) 2014-04-17 2023-09-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Cellule hote recombinante pour l'expression de proteines d'interet
EP2952584A1 (fr) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Production de protéines améliorée
US20170327834A1 (en) 2014-12-15 2017-11-16 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
CN107109414A (zh) 2014-12-23 2017-08-29 先正达参股股份有限公司 鞘翅目有害生物的生物控制
US11154594B2 (en) 2015-04-10 2021-10-26 Syngenta Participations Ag Animal feed compositions and methods of use
WO2018076335A1 (fr) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions et procédés pour améliorer la tolérance au stress abiotique
AU2018241920B2 (en) 2017-03-29 2022-04-21 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition
WO2018213771A1 (fr) 2017-05-18 2018-11-22 Cargill, Incorporated Système d'édition de génome
MX2020003414A (es) 2017-10-02 2020-07-20 Syngenta Participations Ag Proteinas pesticidas modificadas y metodos para controlar las plagas de plantas.
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
EP3927168B1 (fr) 2019-02-20 2025-09-03 Syngenta Crop Protection AG Protéines pesticides modifiées et procédés de lutte contre les parasites des plantes
AU2022220827A1 (en) 2021-02-12 2023-09-21 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion

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EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
WO1993021334A1 (fr) * 1992-04-13 1993-10-28 Zeneca Limited Produits de recombinaison d'adn et plantes les incorporant
WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
WO1993021334A1 (fr) * 1992-04-13 1993-10-28 Zeneca Limited Produits de recombinaison d'adn et plantes les incorporant
WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Non-Patent Citations (1)

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Title
LOCKINGTON, R.A. ET AL.: "Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans", GENE, vol. 33, 1985, pages 137 - 149, XP002022378 *

Also Published As

Publication number Publication date
GB9516241D0 (en) 1995-10-11
AP9801194A0 (en) 1998-03-31
JPH11510694A (ja) 1999-09-21
SK16998A3 (en) 1998-09-09
AP863A (en) 2000-08-04
CA2227445A1 (fr) 1997-02-20
CZ36998A3 (cs) 1998-06-17
EP0846179A2 (fr) 1998-06-10
AU6625296A (en) 1997-03-05
AU704172B2 (en) 1999-04-15
WO1997006268A2 (fr) 1997-02-20
KR19990036251A (ko) 1999-05-25
MX9801008A (es) 1998-04-30
NZ313724A (en) 1999-04-29
HUP9900057A3 (en) 2001-06-28
HUP9900057A2 (hu) 1999-04-28
AR002914A1 (es) 1998-04-29
PL324880A1 (en) 1998-06-22
CN1199425A (zh) 1998-11-18
IL123172A0 (en) 1998-09-24
TR199800177T1 (xx) 1998-05-21
BR9609873A (pt) 1999-03-23

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