WO1997012975B1 - Proteine epitheliale, adn en provenant, et leur utilisation pour la detection precoce du cancer - Google Patents
Proteine epitheliale, adn en provenant, et leur utilisation pour la detection precoce du cancerInfo
- Publication number
- WO1997012975B1 WO1997012975B1 PCT/US1996/015825 US9615825W WO9712975B1 WO 1997012975 B1 WO1997012975 B1 WO 1997012975B1 US 9615825 W US9615825 W US 9615825W WO 9712975 B1 WO9712975 B1 WO 9712975B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- probe
- epithelial
- labeled
- nucleic acid
- protein
- Prior art date
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract 4
- 201000011510 cancer Diseases 0.000 title claims abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 10
- 102000004169 proteins and genes Human genes 0.000 title abstract 10
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 claims abstract 11
- 108010019372 Heterogeneous-Nuclear Ribonucleoproteins Proteins 0.000 claims abstract 6
- 102000006479 Heterogeneous-Nuclear Ribonucleoproteins Human genes 0.000 claims abstract 6
- 108020004999 messenger RNA Proteins 0.000 claims abstract 3
- 239000000523 sample Substances 0.000 claims 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 150000007523 nucleic acids Chemical group 0.000 claims 6
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 2
- 230000003287 optical effect Effects 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
- 239000002853 nucleic acid probe Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 abstract 3
- 201000005202 lung cancer Diseases 0.000 abstract 3
- 208000020816 lung neoplasm Diseases 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 238000000746 purification Methods 0.000 abstract 2
- 208000005623 Carcinogenesis Diseases 0.000 abstract 1
- 238000011537 Coomassie blue staining Methods 0.000 abstract 1
- 241000124008 Mammalia Species 0.000 abstract 1
- 206010035226 Plasma cell myeloma Diseases 0.000 abstract 1
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 210000000988 bone and bone Anatomy 0.000 abstract 1
- 210000000481 breast Anatomy 0.000 abstract 1
- 210000000424 bronchial epithelial cell Anatomy 0.000 abstract 1
- 230000036952 cancer formation Effects 0.000 abstract 1
- 231100000504 carcinogenesis Toxicity 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 210000002919 epithelial cell Anatomy 0.000 abstract 1
- 108010025934 hnRNP A2 Proteins 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 238000011532 immunohistochemical staining Methods 0.000 abstract 1
- 238000012744 immunostaining Methods 0.000 abstract 1
- 238000001155 isoelectric focusing Methods 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 210000004072 lung Anatomy 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 201000001441 melanoma Diseases 0.000 abstract 1
- 238000007479 molecular analysis Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 201000000050 myeloid neoplasm Diseases 0.000 abstract 1
- 210000001672 ovary Anatomy 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 210000002307 prostate Anatomy 0.000 abstract 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 238000001262 western blot Methods 0.000 abstract 1
Abstract
La présente invention concerne une protéine épithéliale purifiée et isolée, un peptide issu de cette protéine et certaines de ses variantes, la présence accrue de ces éléments dans une cellule épithéliale étant l'indication d'un précancer. En l'occurrence, une protéine épithéliale qui constitue une détection précoce marquée pour le cancer du poumon a été purifiée à partir de deux lignées cellulaires de cancer du poumon humain, NCI-H720 et NCI-H157. Grâce à une procédure en six opérations, on a purifié la protéine épithéliale au moyen d'un système de détection par tampons Western sous des conditions non réductrices ainsi que sous des conditions réductrices. Les opérations de purification incluaient une chromatographie sur colonne d'échange anionique, une chromatographie préparative à focalisation isoélectrique, une chromatographie liquide haute performance du radical C18 sur base polymère et une chromatographie liquide haute performance analytique du radical C4. Après une purification d'approximativement 25 000 fois, la protéine de coloration immunologique était pure à plus de 90 % d'après le test de coloration au bleu de Coomassie après réduction de la PAGE SDS. La protéine épithéliale primaire partage une certaine homologie de séquence avec la ribonucléoprotéine A2 nucléaire hétérogène (hnRNP). Une protéine épithéliale mineure de co-purification partage une certaine homologie de séquence avec la variante d'épissage hnRNP-B1. L'analyse moléculaire des cultures de cellules normales de l'épithélium bronchique a mis en évidence un bas niveau d'expression de protéine épithéliale, en concordance avec la coloration immuno-histochimique des échantillons cliniques pour un niveau accru de l'expression dans la plupart des cellules du cancer du poumon. Cette protéine épithéliale est un marqueur de la transformation épithéliale du poumon, du sein, de l'os, de l'ovaire, de la prostate, du rein, des mélanomes et myélomes, et est susceptible d'être en liaison avec le processus de carcinogenèse. L'invention concerne également des procédés de surveillance de l'expression de la protéine épithéliale, du peptide issu de cette protéine et certaines de ses variantes par utilisation de techniques moléculaires et immunologiques pour la recherche du cancer ou du précancer chez les mammifères. L'invention concerne enfin un procédé de diagnostic informatisé du cancer ou du précancer par détection des niveaux d'ARN messager du hnRNP.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96936141A EP0861323A1 (fr) | 1995-10-02 | 1996-10-02 | Proteine epitheliale, adn en provenant, et leur utilisation pour la detection precoce du cancer |
| JP9514401A JP2000500322A (ja) | 1995-10-02 | 1996-10-02 | 初期癌検出への使用のための上皮タンパク質及びそのｄｎａ |
| AU73864/96A AU7386496A (en) | 1995-10-02 | 1996-10-02 | An epithelial protein and dna thereof for use in early cancer detection |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/538,711 US5994062A (en) | 1995-10-02 | 1995-10-02 | Epithelial protein and DNA thereof for use in early cancer detection |
| US08/538,711 | 1995-10-02 | ||
| US08/725,027 | 1996-10-02 | ||
| US08/725,027 US6251586B1 (en) | 1995-10-02 | 1996-10-02 | Epithelial protein and DNA thereof for use in early cancer detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997012975A1 WO1997012975A1 (fr) | 1997-04-10 |
| WO1997012975B1 true WO1997012975B1 (fr) | 1997-05-22 |
Family
ID=27065902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/015825 WO1997012975A1 (fr) | 1995-10-02 | 1996-10-02 | Proteine epitheliale, adn en provenant, et leur utilisation pour la detection precoce du cancer |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6251586B1 (fr) |
| EP (2) | EP1426381A1 (fr) |
| JP (1) | JP2000500322A (fr) |
| AU (1) | AU7386496A (fr) |
| WO (1) | WO1997012975A1 (fr) |
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| US5914238A (en) | 1996-06-05 | 1999-06-22 | Matritech, Inc. | Materials and methods for detection of breast cancer |
| WO1998014469A2 (fr) * | 1996-10-02 | 1998-04-09 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Proteine epitheliale et son adn pour la detection precoce du cancer |
| US6025470A (en) * | 1997-06-23 | 2000-02-15 | Ludwig Institute For Cancer Research | Isolated nona- and decapeptides which bind to HLA molecules, and the use thereof |
| EP1310556A3 (fr) * | 1998-03-31 | 2003-07-23 | Genzyme Corporation | Compositions et méthodes d'identification de cellules de tumeur du poumon |
| EP1790717A3 (fr) * | 1998-03-31 | 2007-06-06 | Genzyme Corporation | Compositions et procédés d'identification de cellules cancéreuses du poumon |
| US7475048B2 (en) * | 1998-05-01 | 2009-01-06 | Health Discovery Corporation | Pre-processed feature ranking for a support vector machine |
| US7805388B2 (en) * | 1998-05-01 | 2010-09-28 | Health Discovery Corporation | Method for feature selection in a support vector machine using feature ranking |
| GB2354241A (en) * | 1999-09-17 | 2001-03-21 | Rupert Donald Holms | Regulatory/unfolding peptides of ezrin |
| AU3887601A (en) * | 1999-09-22 | 2001-04-24 | Cedars-Sinai Medical Center | Nucleic acids encoding vitamin d response element binding proteins, products related thereto, and methods of using same |
| US7970718B2 (en) * | 2001-05-18 | 2011-06-28 | Health Discovery Corporation | Method for feature selection and for evaluating features identified as significant for classifying data |
| CA2404658A1 (fr) * | 2000-03-29 | 2001-10-04 | Ismail Moarefi | Forme spatiale de polypeptides contenant un motif a structure tpr, a fonction de liaison de type chaperon, ses cristaux et ses composes pour inhiber des polypeptides de ce type |
| US6519355B2 (en) * | 2001-03-28 | 2003-02-11 | Alan C. Nelson | Optical projection imaging system and method for automatically detecting cells having nuclear and cytoplasmic densitometric features associated with disease |
| US6522775B2 (en) | 2001-03-28 | 2003-02-18 | Alan C. Nelson | Apparatus and method for imaging small objects in a flow stream using optical tomography |
| US20060023219A1 (en) * | 2001-03-28 | 2006-02-02 | Meyer Michael G | Optical tomography of small objects using parallel ray illumination and post-specimen optical magnification |
| US6591003B2 (en) | 2001-03-28 | 2003-07-08 | Visiongate, Inc. | Optical tomography of small moving objects using time delay and integration imaging |
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| KR100437887B1 (ko) * | 2001-06-29 | 2004-07-03 | 주식회사 인트론바이오테크놀로지 | 크로마토그래피의 원리를 이용한 웨스턴 블롯 분석키트 |
| US6741730B2 (en) | 2001-08-10 | 2004-05-25 | Visiongate, Inc. | Method and apparatus for three-dimensional imaging in the fourier domain |
| US6636623B2 (en) * | 2001-08-10 | 2003-10-21 | Visiongate, Inc. | Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease |
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| US20050085708A1 (en) * | 2002-04-19 | 2005-04-21 | University Of Washington | System and method for preparation of cells for 3D image acquisition |
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| US7738945B2 (en) * | 2002-04-19 | 2010-06-15 | University Of Washington | Method and apparatus for pseudo-projection formation for optical tomography |
| US7811825B2 (en) * | 2002-04-19 | 2010-10-12 | University Of Washington | System and method for processing specimens and images for optical tomography |
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| JPWO2006011667A1 (ja) * | 2004-07-30 | 2008-05-01 | 東ソー株式会社 | ヘテロ核リボヌクレオチドタンパク質Ｂ１（ｈｎＲＮＰＢ１）ｍＲＮＡの測定方法 |
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| US7787112B2 (en) * | 2007-10-22 | 2010-08-31 | Visiongate, Inc. | Depth of field extension for optical tomography |
| US8143600B2 (en) | 2008-02-18 | 2012-03-27 | Visiongate, Inc. | 3D imaging of live cells with ultraviolet radiation |
| US8090183B2 (en) | 2009-03-12 | 2012-01-03 | Visiongate, Inc. | Pattern noise correction for pseudo projections |
| US8254023B2 (en) * | 2009-02-23 | 2012-08-28 | Visiongate, Inc. | Optical tomography system with high-speed scanner |
| CN102687061B (zh) | 2009-10-19 | 2014-12-10 | 文塔纳医疗系统公司 | 成像系统和技术 |
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| EP2582842A2 (fr) | 2010-06-16 | 2013-04-24 | Yissum Research Development Company of The Hebrew University of Jerusalem | Procédé de diagnostic et de traitement du cancer |
| US8113412B1 (en) | 2011-05-13 | 2012-02-14 | Taiwan Semiconductor Manufacturing Company, Ltd | Methods for detecting defect connections between metal bumps |
| BR112017001694A2 (pt) | 2014-08-07 | 2017-11-21 | Procter & Gamble | dose unitária solúvel que compreende uma composição detergente para lavagem de roupas |
| US11069054B2 (en) | 2015-12-30 | 2021-07-20 | Visiongate, Inc. | System and method for automated detection and monitoring of dysplasia and administration of immunotherapy and chemotherapy |
| CA3064785A1 (fr) | 2017-05-30 | 2018-12-06 | The University Of British Columbia | Epitopes dans le motif 1 de reconnaissance d'arn (rrm1) de tdp-43 et anticorps selectifs de mauvais repliement associes |
| US20200306408A1 (en) | 2019-03-28 | 2020-10-01 | The Procter & Gamble Company | Cyclodextrin containing freshening composition |
| WO2021076683A1 (fr) | 2019-10-15 | 2021-04-22 | The Procter & Gamble Company | Compositions détergentes |
| CN113936738B (zh) * | 2021-12-14 | 2022-04-22 | 鲁东大学 | 一种基于卷积神经网络的rna-蛋白质结合位点预测方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4569788A (en) | 1983-05-18 | 1986-02-11 | The United States Of America As Represented By The Department Of Health And Human Services | Monoclonal antibodies against non small cell lung cancer |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| AU632469B2 (en) | 1988-04-04 | 1993-01-07 | Johns Hopkins University, The | A method for early detection of lung cancer |
| US5194599A (en) | 1988-09-23 | 1993-03-16 | Gilead Sciences, Inc. | Hydrogen phosphonodithioate compositions |
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-
1996
- 1996-10-02 JP JP9514401A patent/JP2000500322A/ja active Pending
- 1996-10-02 EP EP03078938A patent/EP1426381A1/fr not_active Withdrawn
- 1996-10-02 EP EP96936141A patent/EP0861323A1/fr not_active Withdrawn
- 1996-10-02 US US08/725,027 patent/US6251586B1/en not_active Expired - Fee Related
- 1996-10-02 WO PCT/US1996/015825 patent/WO1997012975A1/fr not_active Application Discontinuation
- 1996-10-02 AU AU73864/96A patent/AU7386496A/en not_active Abandoned
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