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WO1997013853A3 - Detection de proteines - Google Patents

Detection de proteines Download PDF

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Publication number
WO1997013853A3
WO1997013853A3 PCT/EP1996/004510 EP9604510W WO9713853A3 WO 1997013853 A3 WO1997013853 A3 WO 1997013853A3 EP 9604510 W EP9604510 W EP 9604510W WO 9713853 A3 WO9713853 A3 WO 9713853A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
eukaryotic organism
screened
host cells
dna
Prior art date
Application number
PCT/EP1996/004510
Other languages
English (en)
Other versions
WO1997013853A2 (fr
Inventor
Den Broeck Henriette Catha Van
Graaff Leendert Hendrik De
Jacob Visser
Ooyen Albert Johannes Jose Van
Original Assignee
Gist Brocades Bv
Den Broeck Henriette Catharina
Graaff Leendert Hendrik De
Jacob Visser
Ooyen Albert Johannes Joseph V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Bv, Den Broeck Henriette Catharina, Graaff Leendert Hendrik De, Jacob Visser, Ooyen Albert Johannes Joseph V filed Critical Gist Brocades Bv
Priority to EP96934722A priority Critical patent/EP0796328A2/fr
Priority to JP9514730A priority patent/JPH10510720A/ja
Priority to AU72943/96A priority patent/AU7294396A/en
Publication of WO1997013853A2 publication Critical patent/WO1997013853A2/fr
Publication of WO1997013853A3 publication Critical patent/WO1997013853A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un procédé d'identification d'un fragment d'ADN codant une protéine à analyser. D'après ce procédé, on crible une banque d'ADNc, se présentant sous forme de cellules hôtes bactériennes transformées par de l'ADN obtenu dans un organisme eucaryote capable de produire ladite protéine, ceci afin de déceler l'expression de ladite protéine à l'aide d'un test qui permet de révéler sa présence. Ce procédé se caractérise par le fait que le criblage des cellules hôtes se fait une fois que l'ADN de transformation est devenu partie d'un plasmide. La banque d'ADNc provient de préférence d'un organisme eucaryote. Il est souhaitable que cet organisme eucaryote soit un champignon ou une cellule de levure, de préférence une espèce Aspergillus, et idéalement, un élément du groupe Aspergillus niger. Les enzymes ainsi criblés consistent, de préférence, en des enzymes présentant une activité de dégradation d'arabinoxylane, d'endoxylanase ou de cellulase.
PCT/EP1996/004510 1995-10-13 1996-10-14 Detection de proteines WO1997013853A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP96934722A EP0796328A2 (fr) 1995-10-13 1996-10-14 Detection de proteines
JP9514730A JPH10510720A (ja) 1995-10-13 1996-10-14 タンパク質検出法
AU72943/96A AU7294396A (en) 1995-10-13 1996-10-14 Protein detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95202777.9 1995-10-13
EP95202777 1995-10-13

Publications (2)

Publication Number Publication Date
WO1997013853A2 WO1997013853A2 (fr) 1997-04-17
WO1997013853A3 true WO1997013853A3 (fr) 1997-06-19

Family

ID=8220719

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/004510 WO1997013853A2 (fr) 1995-10-13 1996-10-14 Detection de proteines

Country Status (4)

Country Link
EP (1) EP0796328A2 (fr)
JP (1) JPH10510720A (fr)
AU (1) AU7294396A (fr)
WO (1) WO1997013853A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0976838A1 (fr) 1998-05-06 2000-02-02 Rhone-Poulenc Nutrition Animale Mélange d'enzymes
DE69922978T2 (de) 1998-10-06 2005-12-08 Emalfarb, Mark Aaron, Jupiter Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
FR2786784B1 (fr) * 1998-12-04 2003-01-24 Lesaffre & Cie FRAGMENT D'ADN CODANT POUR LA XYLANASE THERMOSTABLE XynA DE THERMOASCUS
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
AU782105B2 (en) * 2000-04-13 2005-07-07 Dyadic International (Usa), Inc. Novel expression-regulating sequences and expression products in the field of filamentous fungi
DK2298904T3 (en) 2002-06-14 2017-09-11 Bp Corp North America Inc XYLANASES, NUCLEIC ACIDS, CODING THEM, AND METHODS FOR THE PREPARATION AND USE OF THEM
DK1862539T3 (da) 2003-05-29 2012-05-14 Danisco Us Inc Nye trichodermagener
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
EP3406621A1 (fr) 2006-02-14 2018-11-28 BP Corporation North America Inc. Xylanases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation
US20100040595A1 (en) 2006-09-29 2010-02-18 Novozymes A/S Xylanses for animal feed
DK2708602T3 (da) 2007-10-03 2019-06-03 Bp Corp North America Inc Xylanaser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse af dem
CN102414323B (zh) * 2009-04-22 2015-07-08 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的重组多肽的方法
WO2010129287A2 (fr) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Enzymes de dégradation d'hémicellulose
MX2013009762A (es) * 2011-02-25 2013-12-16 Univ Saskatchewan Proteinas para biocontrol de hongos patogenos de plantas.
EA201790153A3 (ru) * 2011-09-14 2017-09-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Ферменты

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463706A1 (fr) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Clonage et expression de gènes de xylanases d'origine fongique
EP0506190A1 (fr) * 1991-03-27 1992-09-30 Gist-Brocades N.V. Clonage et expression de gènes codant pour des enzymes d'origine fongique dégradant l'arabinane
WO1993011249A1 (fr) * 1991-12-04 1993-06-10 Novo Nordisk A/S PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463706A1 (fr) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Clonage et expression de gènes de xylanases d'origine fongique
EP0506190A1 (fr) * 1991-03-27 1992-09-30 Gist-Brocades N.V. Clonage et expression de gènes codant pour des enzymes d'origine fongique dégradant l'arabinane
WO1993011249A1 (fr) * 1991-12-04 1993-06-10 Novo Nordisk A/S PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
H. HESSE ET AL.: "An improved method for generating subtracted cDNA libraries using phage lambda vectors", NUCLEIC ACIDS RESEARCH, vol. 23, no. 16, 25 August 1995 (1995-08-25), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 3355 - 3356, XP002024451 *
J.M. SHORT ET AL.: "Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties", NUCLEIC ACIDS RESEARCH, vol. 16, no. 15, 1988, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 7583 - 7600, XP000568110 *
K.K. STANLEY AND J.P. LUZIO: "Construction of a new bacterial expression vectors: identification of cDNA clones coding for human liver proteins", EMBO J., vol. 3, no. 6, June 1984 (1984-06-01), OXFORD UNIVERSITY PRESS,GB;, pages 1429 - 1434, XP000568110 *
KORMELINK F J M ET AL: "PURIFICATION AND CHARACTERIZATION OF A (1,4)-BETA-D-ARABINOXYLAN ARABINOFURANOHYDROLASE FROM ASPERGILLUS AWAMORI", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 35, no. 6, 1 September 1991 (1991-09-01), pages 753 - 758, XP000567078 *
P. KARLOVSKY AND G.A. WOLF: "Screening of expression cDNA libraries for polysaccharide hydrolysing enzymes: isolation of cDNA clones encoding cellulase, xylanase, and beta-1,3-glucanase of Phytophora parasitica", METHODS IN MOLECULAR AND CELLULAR BIOLOGY, vol. 4, no. 1, 1993, WILEY-LISS, INC., US, pages 40 - 45, XP000567054 *
Y. GOTO ET AL.: "A novel human insulinoma-associated cDNA, IA-1, encodes a protein with "zinc-finger" DNA-binding motifs", J. BIOL. CHEM., vol. 267, no. 21, 25 July 1992 (1992-07-25), AM. SOC. BIOCHEM. MOL.BIOL.,INC.,BALTIMORE,US, pages 15252 - 15257, XP002024452 *

Also Published As

Publication number Publication date
JPH10510720A (ja) 1998-10-20
WO1997013853A2 (fr) 1997-04-17
EP0796328A2 (fr) 1997-09-24
AU7294396A (en) 1997-04-30

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