[go: up one dir, main page]

WO1997015394A1 - Plaques a micropuits - Google Patents

Plaques a micropuits Download PDF

Info

Publication number
WO1997015394A1
WO1997015394A1 PCT/US1996/017072 US9617072W WO9715394A1 WO 1997015394 A1 WO1997015394 A1 WO 1997015394A1 US 9617072 W US9617072 W US 9617072W WO 9715394 A1 WO9715394 A1 WO 9715394A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
wells
multiwell plate
liquid
well
Prior art date
Application number
PCT/US1996/017072
Other languages
English (en)
Other versions
WO1997015394A9 (fr
Inventor
William Arthur Bains
Peter John Houzego
Original Assignee
Smithkline Beecham Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Beecham Corporation filed Critical Smithkline Beecham Corporation
Priority to JP9516786A priority Critical patent/JP2000500567A/ja
Priority to EP96936933A priority patent/EP0862497A4/fr
Publication of WO1997015394A1 publication Critical patent/WO1997015394A1/fr
Publication of WO1997015394A9 publication Critical patent/WO1997015394A9/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks

Definitions

  • This invention relates to microwell plates.
  • HTS High Throughput Screening
  • CC Combinatorial Chemistry
  • This patent application describes a system specifically designed for the parallel synthesis or assay of tens of thousands of compounds.
  • the heart of the concept is a special well structure, called a JetWell in which the wells are arranged as a matrix within the flat plate as is conventional for microwell plates.
  • the novel feature of this concept is that the wells are fabricated with large open areas at one end to accept liquid and integral nozzles at the other end from which liquid can be jet dispensed.
  • These arrays might be made compatible with conventional 96 or 384 microwell plates but advantageously could be higher density (eg 60 x 40 or 120 x 80).
  • the walls of the lower end of the cell may be coated with a binding surface on which the compounds to be synthesised can be attached.
  • JetWell The design of the JetWell enables it to receive and deliver accurate micro volumes of fluid.
  • Dispensing of fluids required for the synthesis into each JetWell would preferably be made without contact of the dispenser to the liquid in the well by using a multinozzle ink-jet head firing into the large end of the wells.
  • a multinozzle approach is necessary to ensure the dispense time for the complete array is short compared to the typical reaction time for each stage of the processing.
  • Liquid removal from the cells is achieved through the integral nozzle in the bottom of the well. During normal operation liquid is prevented from flowing out the cell through the nozzle by surface tension or even a physical plug. Liquid is ejected from the nozzle by applying pressure to the liquid in the wells causing it to jet cleanly out of the hole away from the lower surface.
  • the volume to be removed from the well can be controlled by the pressure applied and time for which it is applied. To achieve clean jetting of the fluid pressure typically in the range 0.1 to 10.0 bar gauge is used and the pressure is applied and removed rapidly to prevent any dribbling of liquid out of the nozzle. If a matching JetWell array is positioned beneath the first array transfer of an accurate volume of liquid from one array to the other occurs in a few milliseconds.
  • the approach provides an accurate system for the parallel dispensing into and from very small JetWell arrays. This enables the volume of the wells to be reduced and the number per plate increased thus greatly increasing the throughput of HTS or CC systems.
  • JetWell Array Structure The specific feature of a JetWell which is different from other forms of microwell plate is that each well has one or more nozzles in the base through which liquid in the well will jet when pressure is applied to the top surface.
  • the diameter of the nozzle controls the rate of liquid flow and is chosen to optimise for speed and accuracy. For example, if the pressure duration is controlled to 1 millisecond accuracy and the volumetric control requirement is 1 nanolitre the flow rate through the hole must be less than 1 microlitre/second. Assuming a jet velocity of 5 metres/second the nozzle would be around 50 microns in diameter which is comparable with that used in conventional ink jet printers.
  • each well depends upon the user requirements.
  • a system might be designed to be compatible with conventional 384 well plates which have a well volume around 20 microlitres arranged on a 4.5mm pitch.
  • well volumes around 20 microlitres arranged on a 4.5mm pitch.
  • a plate of similar size to a conventional microwell plate (either 96 or 384) with wells at 1mm pitch would contain a 96 x 64 array. Taking this size as an example the diameter at the top might be 0.8mm and the hole at the base 30 microns in diameter.
  • Figure 1 shows an example of the basic structure of the JetWell concept and Figure IA is a detailed view, to an enlarged scale of part of that structure.
  • This application, as well as describing the concept and example JetWell designs also addresses suitable manufacturing techniques for the plate and for the associated system components.
  • each line of wells by bonding two strips together with etched structures in them to form the wells.
  • the complete array could be formed etched into the surface of a plate which is then cut into strips and laminated together to form the finished structure.
  • Such a structure could be manufactured for example in glass using a low temperature glass enamel bonding.
  • a single crystal silicon wafer substrate is appropriate with the wells formed by crystallographic etching which can be used to form the nozzle hole with a precision of a few microns.
  • the JetWell concept has many wells in an array on a plate each with a nozzle hole in the base. It is possible to transfer a measured dose from all wells on a JetWell plate to a second receiver JetWell plate in one operation. This is achieved by placing the receiver plate directly below the plate containing the liquid to be dispensed and applying a pressure pulse to all wells of the top plate. Liquid is then jetted from each well on the top plate to the corresponding well on the bottom plate. To jet the fluid cleanly from the nozzle hole the pressure in the fluid must rise quickly and fall sufficiently fast to prevent dribbling from the nozzle. The dynamics of this are well understood from ink-jet experience and it is known that it requires rates in excess of 10 s Pa s "1 (ie 1 bar/ms) for typical dimensions.
  • the pressure rise of a few bar can then be achieved by moving the piston a few millimetres at speeds of a few metres per second.
  • electromagnetic actuators are the simplest.
  • the force required to reach 10 bar is 10 4 Newton's (— 1 ton) which is more difficult to achieve using a simple solenoid drives.
  • an impact actuator can be used.
  • the piston is sealed to the dispense JetWell plate using a compliant seal which permits sufficient travel to reach the desired pressure and then prevents over pressure by limiting the motion using a physical stop.
  • the receiver JetWell plate is fitted directly below the dispense plate.
  • the piston and the two JetWell plates are mounted together on a spring support.
  • the back of the piston is then struck by a mass at a predetermined velocity.
  • the mass striking the piston back moves the piston towards the plate until it reaches the stop. This compresses the air volume above the wells to the desired pressure very quickly. Both the impact mass and the plates continue to move compressing the support spring.
  • the spring decelerates the assembly and then accelerates it back-up.
  • FIG 3 shows two JetWell plates, each of the type shown in Figure 1, mounted in the impact actuator.
  • JWL is the JetWell plate containing the liquid to be dispensed and
  • JW2 is the JetWell plate into which the aliquots are to be dispensed.
  • JW1 and JW2 are mounted rigidly together with JW1 above JW2.
  • the piston plate PP is mounted with a small gap above JW1 on a compressible gas tight edge gasket EG.
  • JW1, JW2 and PP are all supported on spring support SS.
  • mass M is accelerated towards PP so that it impacts at a specified velocity.
  • the piston plate PP and mass M move towards JW1 compressing the edge gasket (EG) until the motion stop (MSI) stops it at a preset gap.
  • EG edge gasket
  • MSI motion stop
  • the dispense pressure is a function of air volume and the distance to the forward stop and the dispense time is a function of the spring constants, masses and initial impact velocity.
  • the technique therefore provides the ability to independently set time and pressure for dispensing over a wide range.
  • a small area piston pump can be located over the specific well.
  • lines, or rectangular sections of the plate can be addressed by specifically shaped actuators.
  • the use of a minimal volume positive displacement air pressure generator has important advantages as has the impact technique described to implement it.
  • the technique for dispensing from a JetWell plate can also be used for dispensing to a JetWell plate providing the same volume is required in each well. This is applicable in some, but not all instances. Where different amounts are required in each well, alternative approaches can be used.
  • Nozzle plates could be selected from a library and cleaned for re-use
  • programmable nozzle arrays including but not restricted to solenoid, piezo, mechanically and thermal drives for ball, plate, disc or poppet mechamsms.
  • nozzles in the sub 0.1mm diameter range with minimal dead volume and implementable in arrays of many thousand is a challenge.
  • One approach is to use cone shaped nozzles which are produced as an array in a ferro magnetic material.
  • the nozzle plate is removable from the pressure generating head.
  • To programme the array to the required pattern magnetic beads are placed in those nozzles to be closed. The magnetic attraction to the plate holds them in place whilst the plate is replaced in the dispense head.
  • the nozzles are then filled with the liquid to be dispensed and a pressure pulse applied for the required time.
  • the ball in cone arrangement ensures a good seal against the dispense pressure.
  • the magnetic beads may be disposable or reusable depending upon application. By using several plates which can be programmed whilst other operations are being performed the system will be rapid and flexible.
  • JetWells of the dispense plate are prefilled with the volume and type of liquids required using an accurate but not necessarily fast technique.
  • the liquid is held in the wells by surface tension.
  • the plate is ready for use. It is positioned over the reaction JetWell plate, and all the content of each well is jet dispensed by a pressure pulse as described previously.
  • JetWell dispensing approaches In some cases neither of these JetWell dispensing approaches will be appropriate. For these instances it may be necessary to use a precision dispensing head.
  • the technique most suitable for this is based upon drop-on-demand ink-jet systems where the dose in each well can be set by dispensing a measured number of drops.
  • All material in contact with the fluid must be inert to a wide range of acids, alkalis and solvents. Preferably it should be restricted to silicon dioxide, stainless steel, and inert polymers
  • the system must be easy to fill and to flush as it must be used many times with a wide range of fluids
  • the nozzle diameter is chosen to match the well size. Typically the drop size is selected to be in the range 10° to IO "4 of the well volume
  • Typical delivery rates are in the range IO 3 to 10 4 drops per second
  • Actuation will preferably be by a thin piezo layer bonded to a silicon or silicon dioxide membrane.
  • the piezo drivers may be integrated on to the head.
  • the head would have gold contacts deposited enabling it to make electrical contact to off head drivers.
  • the application lends itself to implementation of the head using silicon crystallographic etching techniques to form the structure and either silicon to glass or silicon to silicon bonding for assembly.
  • top shooter or side shooter forms could be implemented at typical pitches required (-*0.5mm).
  • the best approach for achieving precision is therefore to include a technique for the calibration of each device prior to use.
  • a technique for the calibration of each device prior to use For example, for JetWell plates there may be a distribution of nozzle diameters coupled with a pressure variation which leads to different volumes being dispensed for each well. If a quantative assay is being performed on part-samples from each well, volume variation would influence the result.
  • the system could be calibrated before use by dispensing a reagent which can be used in a simple standard assay test for volume.
  • a reagent which can be used in a simple standard assay test for volume.
  • This for example could be a colour change reaction which is convenient for automated measurement.
  • the calibration would be used to correct the results of the real assay rather than to control the volumes dispensed.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Plaque à micropuits dans laquelle les puits présentent une grande ouverture au niveau supérieur et un petit trou d'ajutage dans la base, lequel est choisi pour qu'un jet de liquide soit émis lorsqu'une impulsion de pression est appliquée sur ladite surface, de sorte que la sélection d'un temps donné d'impulsion de pression permette la distribution d'une quantité précise de volume du puits.
PCT/US1996/017072 1995-10-24 1996-10-24 Plaques a micropuits WO1997015394A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP9516786A JP2000500567A (ja) 1995-10-24 1996-10-24 マイクロウエルプレート
EP96936933A EP0862497A4 (fr) 1995-10-24 1996-10-24 Plaques a micropuits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9521775.8A GB9521775D0 (en) 1995-10-24 1995-10-24 Microwell plates
GB9521775.8 1995-10-24

Publications (2)

Publication Number Publication Date
WO1997015394A1 true WO1997015394A1 (fr) 1997-05-01
WO1997015394A9 WO1997015394A9 (fr) 1997-09-25

Family

ID=10782830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/017072 WO1997015394A1 (fr) 1995-10-24 1996-10-24 Plaques a micropuits

Country Status (4)

Country Link
EP (1) EP0862497A4 (fr)
JP (1) JP2000500567A (fr)
GB (1) GB9521775D0 (fr)
WO (1) WO1997015394A1 (fr)

Cited By (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999001221A1 (fr) * 1997-06-30 1999-01-14 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Cuve de microreaction, systeme de cuves de microreaction et procede pour delivrer un liquide contenu dans des cuves de microreaction
WO1999019067A1 (fr) * 1997-10-10 1999-04-22 Biosepra, Inc. Pile multicoupelles a plusieurs plaques alignees et procede de traitement d'echantillons biologiques/chimiques de ladite pile
WO1999034920A1 (fr) * 1998-01-12 1999-07-15 Massachusetts Institute Of Technology Procede pour effectuer des dosages microscopiques
WO1999036176A1 (fr) 1998-01-17 1999-07-22 Central Research Laboratories Limited Appareil permettant de delivrer une quantite predeterminee de liquide
GB2336908A (en) * 1998-04-17 1999-11-03 Merck & Co Inc Multiwell plate
EP0955084A1 (fr) * 1998-04-27 1999-11-10 Corning Incorporated Réservoir capillaire refilé
WO1999060373A1 (fr) * 1998-05-20 1999-11-25 Nanomont Gesellschaft Für Nanotechnologie Mbh Dispositif et procede pour fixer des micro-objets et/ou des nano-objets
WO2000024511A1 (fr) * 1998-10-26 2000-05-04 The Regents Of The University Of California Ensemble tete d'injection/plaque de titrage servant a preparer, stocker et transferer des rangees de micro-gouttes
US6103199A (en) * 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
WO2001019517A1 (fr) * 1999-09-14 2001-03-22 Pamgene, B.V. Dispositif analytique d'essai comprenant un substrat a canaux traversants et procedes d'utilisation et appareil perfectionnes
WO2001030499A1 (fr) * 1999-10-26 2001-05-03 Tibotec Nv Dispositif et procede correspondant de distribution de petits volumes de liquide
WO2001030500A1 (fr) * 1999-10-29 2001-05-03 Gyros Ab Dispositif et distributeur de goutelettes
EP1103305A1 (fr) * 1999-11-26 2001-05-30 F.Hoffmann-La Roche Ag Système de couplage à un macroactionneur faisant partie d'un module de pipettage
WO2001019502A3 (fr) * 1999-09-17 2001-08-09 Millipore Corp Carte tamis de grande capacite
WO2001018539A3 (fr) * 1999-09-06 2001-09-27 Amersham Pharm Biotech Uk Ltd Chambre de melange
EP1142641A1 (fr) * 2000-04-04 2001-10-10 CSEM Centre Suisse d'Electronique et de Microtechnique SA Préparation d'échantillons biologiques au moyen d'une matrice de micro-buses
US6309828B1 (en) 1998-11-18 2001-10-30 Agilent Technologies, Inc. Method and apparatus for fabricating replicate arrays of nucleic acid molecules
WO2001089694A1 (fr) * 2000-05-22 2001-11-29 Biorobotics Limited Dispositif pour transfert de liquides
US6350618B1 (en) 1998-04-27 2002-02-26 Corning Incorporated Redrawn capillary imaging reservoir
FR2816711A1 (fr) * 2000-11-15 2002-05-17 Biomerieux Sa Procede de depot d'une solution d'analyte
EP1206966A1 (fr) * 2000-11-17 2002-05-22 Tecan Trading AG Dispositif d'aspiration et de distribution des échantillons liquides
US6436351B1 (en) 1998-07-15 2002-08-20 Deltagen Research Laboratories, L.L.C. Microtitre chemical reaction system
EP1250955A1 (fr) * 1998-09-08 2002-10-23 Tibotec N.V. Procédé de criblage rapide pour des analytes
US6544480B1 (en) 1999-10-26 2003-04-08 Tibotec Bvba Device and related method for dispensing small volumes of liquid
WO2000020117A3 (fr) * 1998-10-02 2003-04-17 Central Research Lab Ltd Procede et appareil permettant d'extraire une substance d'un contenant
EP1019696A4 (fr) * 1997-09-19 2003-07-23 Aclara Biosciences Inc Systeme et procede de transfert de liquides
EP1188475A3 (fr) * 2000-09-19 2003-07-23 Canon Kabushiki Kaisha Procédé pour la fabrication d'un support d'échantillon et dispositif utilisé dans le procédé
WO2003079030A1 (fr) * 2002-03-14 2003-09-25 Cybio Ag Procede pour caracteriser la technique de manutention de liquides hautement parallelisee, au moyen de microplaques, et kit d'essai pour mettre ledit procede en oeuvre
US6689323B2 (en) 1998-10-30 2004-02-10 Agilent Technologies Method and apparatus for liquid transfer
WO2003074166A3 (fr) * 2002-02-28 2004-04-29 Edc Biosystems Inc Appareil et procede de composition de matieres a haute densite sur des substrats cibles au moyen d'une sequence rapide
US6762061B1 (en) 1998-07-03 2004-07-13 Corning Incorporated Redrawn capillary imaging reservoir
US6824024B2 (en) 2000-11-17 2004-11-30 Tecan Trading Ag Device for the take-up and/or release of liquid samples
WO2004106070A3 (fr) * 2003-05-28 2005-02-10 Hoffmann La Roche Dispositif distributeur de gouttes de liquide
US6884626B1 (en) 1998-04-27 2005-04-26 Corning Incorporated Redrawn capillary imaging reservoir
US6886409B2 (en) 2001-03-13 2005-05-03 Pamgene International B.V. System for controlling the flow of a fluid through a substrate
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
EP1113850A4 (fr) * 1998-09-17 2006-02-01 Advion Biosciences Inc Systeme monolithique integre microfabrique d'electronebulisation et de chromatographie en phase liquide et procede associe
US7037680B2 (en) 1993-09-21 2006-05-02 The United States Of America As Represented By The Secretary Of The Army Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy
US7063216B2 (en) 2003-09-04 2006-06-20 Millipore Corporation Underdrain useful in the construction of a filtration device
EP1269518A4 (fr) * 1999-12-30 2006-06-28 Advion Biosciences Inc Dispositif, systemes et procedes d'electropulverisation multiple
US7081529B2 (en) 1993-09-21 2006-07-25 The United States Of America As Represented By The Secretary Of The Army Recombinant vaccine against botulinum neurotoxin
US7105134B2 (en) 2001-03-13 2006-09-12 Pamgene B.V. Device for holding a substrate
EP1442135A4 (fr) * 2001-10-11 2006-09-13 Xerox Corp Dispositifs et procedes permettant de detecter des sequences genetiques
EP1627680A3 (fr) * 2004-08-17 2006-10-11 Samsung Electronics Co., Ltd. Dispositif type jet d'encre et méthode de dépôt de microréseaux pour la préparation de micromatrices
US7211224B2 (en) 2002-05-23 2007-05-01 Millipore Corporation One piece filtration plate
US7227010B2 (en) 1993-09-21 2007-06-05 United States Of America As Represented By The Secretary Of The Army Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy
EP1248949A4 (fr) * 2000-01-18 2007-07-25 Advion Biosciences Inc Milieu de separation, systeme a buses d'electronebulisation multiples et procede associe
EP1466144A4 (fr) * 2001-12-19 2007-09-05 Sau Lan Tang Staats Elements d'interface et supports pour dispositifs de reseau microfluidique
US7427379B1 (en) 1999-03-19 2008-09-23 Biotage Ab Liquid dispensing apparatus
DE102007041071A1 (de) * 2007-08-30 2009-03-05 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Aufnahme einer Flüssigkeit sowie Vorrichtung zur Aufbringung von Flüssigkeiten auf Probenträger und Verfahren hierzu
US7604983B2 (en) 2000-02-18 2009-10-20 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US8318479B2 (en) 2004-05-19 2012-11-27 Massachusetts Institute Of Technology Perfused three-dimensional cell/tissue disease models
US8354080B2 (en) 2009-04-10 2013-01-15 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US8753588B2 (en) 2003-10-15 2014-06-17 Emd Millipore Corporation Support and stand-off ribs for underdrain for multi-well device
US8900856B2 (en) 2004-04-08 2014-12-02 Biomatrica, Inc. Integration of sample storage and sample management for life science
US9266108B2 (en) 2004-03-12 2016-02-23 Life Technologies Corporation Nanoliter array loading
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
US9428800B2 (en) 2002-12-20 2016-08-30 Life Technologies Corporation Thermal cycling apparatus and method
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US10064404B2 (en) 2014-06-10 2018-09-04 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US10195579B2 (en) 1999-03-19 2019-02-05 Life Technologies Corporation Multi-through hole testing plate for high throughput screening
US10213761B2 (en) 2004-08-04 2019-02-26 Life Technologies Corporation Coating process for microfluidic sample arrays
CN110042060A (zh) * 2014-08-05 2019-07-23 雅马哈发动机株式会社 板
US10568317B2 (en) 2015-12-08 2020-02-25 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
LU501476B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501475B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501473B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for setting a volume of liquid to be dispensed
LU501474B1 (en) * 2022-02-15 2023-08-17 Dispendix Gmbh Method for determining a volume of liquid arranged in a receptacle
LU501663B1 (en) * 2022-03-15 2023-09-20 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501825B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for setting a volume of liquid to be dispensed by using a function
LU501824B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for setting a volume of liquid to be dispensed from a receptacle
LU501823B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU502213B1 (en) * 2022-06-02 2023-12-04 Dispendix Gmbh Method for Determining a Volume of Liquid dispensed from a Receptacle
US12070731B2 (en) 2004-08-04 2024-08-27 Life Technologies Corporation Methods and systems for aligning dispensing arrays with microfluidic sample arrays

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3407068B2 (ja) 1997-12-12 2003-05-19 株式会社日立製作所 化学分析装置
WO2002030561A2 (fr) * 2000-10-10 2002-04-18 Biotrove, Inc. Dispositifs d'essai biologique, de synthese et de stockage, et procedes de fabrication, d'utilisation et de manipulation de tels dispositifs
JP2007526451A (ja) * 2003-06-25 2007-09-13 ウオーターズ・インベストメンツ・リミテツド プラットフォームに沿う交差汚染を防止するのに使用される装置、およびその装置を製造する方法
KR101211123B1 (ko) * 2009-10-15 2012-12-12 포항공과대학교 산학협력단 큐벳
JP5691178B2 (ja) * 2010-01-22 2015-04-01 凸版印刷株式会社 分注方法及び分注装置
TWI417532B (zh) * 2010-03-01 2013-12-01 Univ Nat Chiao Tung 用於多階衝擊器之多微孔噴嘴板之製造方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111754A (en) * 1976-11-29 1978-09-05 Hydow Park Immunological testing devices and methods
US4304865A (en) * 1978-08-31 1981-12-08 National Research Development Corporation Harvesting material from micro-culture plates
US4483925A (en) * 1982-12-30 1984-11-20 Becton, Dickinson And Company Liquid removal device
US4493815A (en) * 1983-07-28 1985-01-15 Bio-Rad Laboratories, Inc. Supporting and filtering biochemical test plate assembly
US4787988A (en) * 1987-02-20 1988-11-29 Biomedical Research And Development Laboratories, Inc. Cell harvester
US5039493A (en) * 1990-05-04 1991-08-13 The United States Of America As Represented By The Secretary Of The Navy Positive pressure blotting apparatus with hydropholic filter means
US5395594A (en) * 1991-08-26 1995-03-07 Shimadzu Corporation Simultaneous multiple chemical synthesizer

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6433070U (fr) * 1987-08-22 1989-03-01
US5159197A (en) * 1988-02-16 1992-10-27 Difco Laboratories Luminescence test and exposure apparatus
SE9002579D0 (sv) * 1990-08-07 1990-08-07 Pharmacia Ab Method and apparatus for carrying out biochemical reactions
US5472672A (en) * 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111754A (en) * 1976-11-29 1978-09-05 Hydow Park Immunological testing devices and methods
US4304865A (en) * 1978-08-31 1981-12-08 National Research Development Corporation Harvesting material from micro-culture plates
US4483925A (en) * 1982-12-30 1984-11-20 Becton, Dickinson And Company Liquid removal device
US4493815A (en) * 1983-07-28 1985-01-15 Bio-Rad Laboratories, Inc. Supporting and filtering biochemical test plate assembly
US4787988A (en) * 1987-02-20 1988-11-29 Biomedical Research And Development Laboratories, Inc. Cell harvester
US5039493A (en) * 1990-05-04 1991-08-13 The United States Of America As Represented By The Secretary Of The Navy Positive pressure blotting apparatus with hydropholic filter means
US5395594A (en) * 1991-08-26 1995-03-07 Shimadzu Corporation Simultaneous multiple chemical synthesizer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0862497A4 *

Cited By (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7227010B2 (en) 1993-09-21 2007-06-05 United States Of America As Represented By The Secretary Of The Army Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy
US7214787B1 (en) 1993-09-21 2007-05-08 United States Of America As Represented By The Secretary Of The Army Recombinant vaccine against botulinum neurotoxin
US7037680B2 (en) 1993-09-21 2006-05-02 The United States Of America As Represented By The Secretary Of The Army Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy
US7081529B2 (en) 1993-09-21 2006-07-25 The United States Of America As Represented By The Secretary Of The Army Recombinant vaccine against botulinum neurotoxin
WO1999001221A1 (fr) * 1997-06-30 1999-01-14 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Cuve de microreaction, systeme de cuves de microreaction et procede pour delivrer un liquide contenu dans des cuves de microreaction
DE19828995B4 (de) * 1997-06-30 2006-01-12 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Anordnung von Mikroreaktionsgefäßen und Verfahren zur Abgabe einer Flüssigkeit aus einer Anordnung von Mikroreaktionsgefäßen
EP1019696A4 (fr) * 1997-09-19 2003-07-23 Aclara Biosciences Inc Systeme et procede de transfert de liquides
WO1999019067A1 (fr) * 1997-10-10 1999-04-22 Biosepra, Inc. Pile multicoupelles a plusieurs plaques alignees et procede de traitement d'echantillons biologiques/chimiques de ladite pile
US6464942B2 (en) 1997-10-10 2002-10-15 Ciphergen Biosystems, Inc. Plate alignment and sample transfer indicia for a multiwell multiplate stack and method for processing biological/chemical samples using the same
US7273759B2 (en) 1997-10-10 2007-09-25 Pall Corporation Plate alignment and sample transfer indicia for a multiwell multiplate stack and method for processing biological/chemical samples using the same
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
JP2009244271A (ja) * 1998-01-12 2009-10-22 Massachusetts Inst Of Technol <Mit> 微量分析方法及び装置
US7547556B2 (en) 1998-01-12 2009-06-16 Massachusetts Institute Of Technology Methods for filing a sample array by droplet dragging
US6387331B1 (en) 1998-01-12 2002-05-14 Massachusetts Institute Of Technology Method and apparatus for performing microassays
US6743633B1 (en) 1998-01-12 2004-06-01 Massachusetts Institute Of Technology Method for performing microassays
WO1999034920A1 (fr) * 1998-01-12 1999-07-15 Massachusetts Institute Of Technology Procede pour effectuer des dosages microscopiques
WO1999036176A1 (fr) 1998-01-17 1999-07-22 Central Research Laboratories Limited Appareil permettant de delivrer une quantite predeterminee de liquide
US6401769B1 (en) 1998-01-17 2002-06-11 Central Research Laboratories Limited Apparatus for dispensing a predetermined volume of a liquid
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
GB2336908A (en) * 1998-04-17 1999-11-03 Merck & Co Inc Multiwell plate
US6596237B1 (en) 1998-04-27 2003-07-22 Nicholas F. Borrelli Redrawn capillary imaging reservoir
EP0955084A1 (fr) * 1998-04-27 1999-11-10 Corning Incorporated Réservoir capillaire refilé
EP1075327A4 (fr) * 1998-04-27 2006-01-25 Corning Inc Reservoir capillaire etire pour imagerie
US6884626B1 (en) 1998-04-27 2005-04-26 Corning Incorporated Redrawn capillary imaging reservoir
US6350618B1 (en) 1998-04-27 2002-02-26 Corning Incorporated Redrawn capillary imaging reservoir
WO1999060373A1 (fr) * 1998-05-20 1999-11-25 Nanomont Gesellschaft Für Nanotechnologie Mbh Dispositif et procede pour fixer des micro-objets et/ou des nano-objets
US6762061B1 (en) 1998-07-03 2004-07-13 Corning Incorporated Redrawn capillary imaging reservoir
US6436351B1 (en) 1998-07-15 2002-08-20 Deltagen Research Laboratories, L.L.C. Microtitre chemical reaction system
EP1250955A1 (fr) * 1998-09-08 2002-10-23 Tibotec N.V. Procédé de criblage rapide pour des analytes
US6103199A (en) * 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
EP1113850A4 (fr) * 1998-09-17 2006-02-01 Advion Biosciences Inc Systeme monolithique integre microfabrique d'electronebulisation et de chromatographie en phase liquide et procede associe
WO2000020117A3 (fr) * 1998-10-02 2003-04-17 Central Research Lab Ltd Procede et appareil permettant d'extraire une substance d'un contenant
WO2000024511A1 (fr) * 1998-10-26 2000-05-04 The Regents Of The University Of California Ensemble tete d'injection/plaque de titrage servant a preparer, stocker et transferer des rangees de micro-gouttes
US6165417A (en) * 1998-10-26 2000-12-26 The Regents Of The University Of California Integrated titer plate-injector head for microdrop array preparation, storage and transfer
US6689323B2 (en) 1998-10-30 2004-02-10 Agilent Technologies Method and apparatus for liquid transfer
DE19950809B4 (de) * 1998-10-30 2007-11-15 Agilent Technologies, Inc. (n.d.Ges.d. Staates Delaware), Santa Clara Verfahren und Vorrichtung für eine Flüssigkeitsübertragung
US6309828B1 (en) 1998-11-18 2001-10-30 Agilent Technologies, Inc. Method and apparatus for fabricating replicate arrays of nucleic acid molecules
US10195579B2 (en) 1999-03-19 2019-02-05 Life Technologies Corporation Multi-through hole testing plate for high throughput screening
US7427379B1 (en) 1999-03-19 2008-09-23 Biotage Ab Liquid dispensing apparatus
WO2001018539A3 (fr) * 1999-09-06 2001-09-27 Amersham Pharm Biotech Uk Ltd Chambre de melange
US6955462B1 (en) 1999-09-06 2005-10-18 Amersham Plc Mixing chamber
US6849408B2 (en) 1999-09-14 2005-02-01 Pamgene B.V. Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same
WO2001019517A1 (fr) * 1999-09-14 2001-03-22 Pamgene, B.V. Dispositif analytique d'essai comprenant un substrat a canaux traversants et procedes d'utilisation et appareil perfectionnes
EP1419819A1 (fr) * 1999-09-14 2004-05-19 PamGene B.V. Méthode d'analyse d'échantillons liquides dans des canaux sur substrats par utilisation de flux alternés
WO2001019502A3 (fr) * 1999-09-17 2001-08-09 Millipore Corp Carte tamis de grande capacite
WO2001030499A1 (fr) * 1999-10-26 2001-05-03 Tibotec Nv Dispositif et procede correspondant de distribution de petits volumes de liquide
US6544480B1 (en) 1999-10-26 2003-04-08 Tibotec Bvba Device and related method for dispensing small volumes of liquid
WO2001030500A1 (fr) * 1999-10-29 2001-05-03 Gyros Ab Dispositif et distributeur de goutelettes
US7125519B1 (en) 1999-11-26 2006-10-24 Roche Diagnostics Corporation Actuator coupling system and a pipetting module comprising such a coupling system
EP1103305A1 (fr) * 1999-11-26 2001-05-30 F.Hoffmann-La Roche Ag Système de couplage à un macroactionneur faisant partie d'un module de pipettage
WO2001037996A1 (fr) * 1999-11-26 2001-05-31 F. Hoffmann-La Roche Ag Systeme de couplage d'un actionneur et module de pipettage le comprenant
EP1269518A4 (fr) * 1999-12-30 2006-06-28 Advion Biosciences Inc Dispositif, systemes et procedes d'electropulverisation multiple
EP1248949A4 (fr) * 2000-01-18 2007-07-25 Advion Biosciences Inc Milieu de separation, systeme a buses d'electronebulisation multiples et procede associe
US10378049B2 (en) 2000-02-18 2019-08-13 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of microvolume liquid reactions
US9518299B2 (en) 2000-02-18 2016-12-13 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US8906618B2 (en) 2000-02-18 2014-12-09 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US7604983B2 (en) 2000-02-18 2009-10-20 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US10227644B2 (en) 2000-02-18 2019-03-12 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of microvolume liquid reactions
EP1142641A1 (fr) * 2000-04-04 2001-10-10 CSEM Centre Suisse d'Electronique et de Microtechnique SA Préparation d'échantillons biologiques au moyen d'une matrice de micro-buses
WO2001089694A1 (fr) * 2000-05-22 2001-11-29 Biorobotics Limited Dispositif pour transfert de liquides
US7731904B2 (en) 2000-09-19 2010-06-08 Canon Kabushiki Kaisha Method for making probe support and apparatus used for the method
EP1188475A3 (fr) * 2000-09-19 2003-07-23 Canon Kabushiki Kaisha Procédé pour la fabrication d'un support d'échantillon et dispositif utilisé dans le procédé
US9248445B2 (en) 2000-09-19 2016-02-02 Canon Kabushiki Kaisha Method for making probe support and apparatus used for the method
WO2002040164A1 (fr) * 2000-11-15 2002-05-23 Biomerieux S.A. Procede de depot d'une solution d'analyte, dispositif de mise en oeuvre de ce procede, support solide fabrique selon le procede et kit de diagnostic comprenant un tell support solide
FR2816711A1 (fr) * 2000-11-15 2002-05-17 Biomerieux Sa Procede de depot d'une solution d'analyte
CH695544A5 (de) * 2000-11-17 2006-06-30 Tecan Trading Ag Vorrichtung zur Abgabe bzw. Aufnahme/Abgabe von Flüssigkeitsproben.
US6824024B2 (en) 2000-11-17 2004-11-30 Tecan Trading Ag Device for the take-up and/or release of liquid samples
WO2002040163A1 (fr) * 2000-11-17 2002-05-23 Tecan Trading Ag Dispositif permettant de prelever et/ou de distribuer des echantillons de liquide
EP1206966A1 (fr) * 2000-11-17 2002-05-22 Tecan Trading AG Dispositif d'aspiration et de distribution des échantillons liquides
US6869571B2 (en) 2000-11-17 2005-03-22 Tecan Trading Ag Device for aspirating and dispensing liquid samples
US7105134B2 (en) 2001-03-13 2006-09-12 Pamgene B.V. Device for holding a substrate
US6886409B2 (en) 2001-03-13 2005-05-03 Pamgene International B.V. System for controlling the flow of a fluid through a substrate
EP1442135A4 (fr) * 2001-10-11 2006-09-13 Xerox Corp Dispositifs et procedes permettant de detecter des sequences genetiques
EP1466144A4 (fr) * 2001-12-19 2007-09-05 Sau Lan Tang Staats Elements d'interface et supports pour dispositifs de reseau microfluidique
WO2003074166A3 (fr) * 2002-02-28 2004-04-29 Edc Biosystems Inc Appareil et procede de composition de matieres a haute densite sur des substrats cibles au moyen d'une sequence rapide
WO2003079030A1 (fr) * 2002-03-14 2003-09-25 Cybio Ag Procede pour caracteriser la technique de manutention de liquides hautement parallelisee, au moyen de microplaques, et kit d'essai pour mettre ledit procede en oeuvre
US7504264B2 (en) 2002-03-14 2009-03-17 Cybio Ag Method for characterizing a highly parallelized liquid handling technique using microplates and test kit for carrying out the method
GB2403535B (en) * 2002-03-14 2005-05-11 Cybio Ag Method for characterizing a highly parallelized liquid handling technique using microplates and test kit for carying out the method
GB2403535A (en) * 2002-03-14 2005-01-05 Cybio Ag Method for characterizing a highly parallelized liquid handling technique using microplates and test kit for carrying out the method
US7211224B2 (en) 2002-05-23 2007-05-01 Millipore Corporation One piece filtration plate
US9428800B2 (en) 2002-12-20 2016-08-30 Life Technologies Corporation Thermal cycling apparatus and method
WO2004106070A3 (fr) * 2003-05-28 2005-02-10 Hoffmann La Roche Dispositif distributeur de gouttes de liquide
US7063216B2 (en) 2003-09-04 2006-06-20 Millipore Corporation Underdrain useful in the construction of a filtration device
US8753588B2 (en) 2003-10-15 2014-06-17 Emd Millipore Corporation Support and stand-off ribs for underdrain for multi-well device
US10065189B2 (en) 2004-03-12 2018-09-04 Life Technologies Corporation Nanoliter array loading
US10974247B2 (en) 2004-03-12 2021-04-13 Life Technologies Corporation Nanoliter array loading
US9266108B2 (en) 2004-03-12 2016-02-23 Life Technologies Corporation Nanoliter array loading
US9078426B2 (en) 2004-04-08 2015-07-14 Biomatrica, Inc. Integration of sample storage and sample management for life science
US8900856B2 (en) 2004-04-08 2014-12-02 Biomatrica, Inc. Integration of sample storage and sample management for life science
US8318479B2 (en) 2004-05-19 2012-11-27 Massachusetts Institute Of Technology Perfused three-dimensional cell/tissue disease models
US12070731B2 (en) 2004-08-04 2024-08-27 Life Technologies Corporation Methods and systems for aligning dispensing arrays with microfluidic sample arrays
US11154834B2 (en) 2004-08-04 2021-10-26 Life Technologies Corporation Coating process for microfluidic sample arrays
US10213761B2 (en) 2004-08-04 2019-02-26 Life Technologies Corporation Coating process for microfluidic sample arrays
EP1627680A3 (fr) * 2004-08-17 2006-10-11 Samsung Electronics Co., Ltd. Dispositif type jet d'encre et méthode de dépôt de microréseaux pour la préparation de micromatrices
DE102007041071B4 (de) * 2007-08-30 2010-02-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Aufnahme einer Flüssigkeit sowie Vorrichtung zur Aufbringung von Flüssigkeiten auf Probenträger und Verfahren hierzu
DE102007041071A1 (de) * 2007-08-30 2009-03-05 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Aufnahme einer Flüssigkeit sowie Vorrichtung zur Aufbringung von Flüssigkeiten auf Probenträger und Verfahren hierzu
US9138744B2 (en) 2009-04-10 2015-09-22 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US8871156B2 (en) 2009-04-10 2014-10-28 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US8354080B2 (en) 2009-04-10 2013-01-15 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US10092902B2 (en) 2009-04-10 2018-10-09 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US9999217B2 (en) 2010-07-26 2018-06-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA, and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US10064404B2 (en) 2014-06-10 2018-09-04 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US10772319B2 (en) 2014-06-10 2020-09-15 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US11672247B2 (en) 2014-06-10 2023-06-13 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US12121022B2 (en) 2014-06-10 2024-10-22 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
CN110042060A (zh) * 2014-08-05 2019-07-23 雅马哈发动机株式会社 板
US10568317B2 (en) 2015-12-08 2020-02-25 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
US11116205B2 (en) 2015-12-08 2021-09-14 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
US12089588B2 (en) 2015-12-08 2024-09-17 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
LU501473B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for setting a volume of liquid to be dispensed
WO2023156354A1 (fr) * 2022-02-15 2023-08-24 Dispendix Gmbh Procédé de détermination d'un volume de liquide disposé dans un récipient
LU501474B1 (en) * 2022-02-15 2023-08-17 Dispendix Gmbh Method for determining a volume of liquid arranged in a receptacle
LU501475B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501476B1 (en) * 2022-02-15 2023-08-16 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501663B1 (en) * 2022-03-15 2023-09-20 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU501825B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for setting a volume of liquid to be dispensed by using a function
LU501824B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for setting a volume of liquid to be dispensed from a receptacle
LU501823B1 (en) * 2022-04-10 2023-10-10 Dispendix Gmbh Method for determining a function for determining a volume of liquid to be dispensed
LU502213B1 (en) * 2022-06-02 2023-12-04 Dispendix Gmbh Method for Determining a Volume of Liquid dispensed from a Receptacle

Also Published As

Publication number Publication date
JP2000500567A (ja) 2000-01-18
GB9521775D0 (en) 1996-01-03
EP0862497A1 (fr) 1998-09-09
EP0862497A4 (fr) 1999-08-11

Similar Documents

Publication Publication Date Title
WO1997015394A1 (fr) Plaques a micropuits
WO1997015394A9 (fr) Plaques a micropuits
JP3598066B2 (ja) フォーマット変換機能を有する流体制御装置
US7717544B2 (en) Method for acoustically ejecting a droplet of fluid from a reservoir by an acoustic fluid ejection apparatus
EP1208913B1 (fr) Système d&#39;éjection d&#39;une multiplicité de gouttes des fluides biologiques
US5658802A (en) Method and apparatus for making miniaturized diagnostic arrays
EP1274508B1 (fr) Dispositif de distribution de petites doses de liquide tres precises
US20100302322A1 (en) Micromachined fluid ejector
US20020168297A1 (en) Method and device for dispensing of droplets
JP3713017B2 (ja) 基板上に微小液滴を非接触で供給する装置および方法
AU746828B2 (en) Apparatus for dispensing a predetermined volume of a liquid
JP2004503389A (ja) 微小滴を並行かつ選択的に分配するための高効率なシステム
US20090033690A1 (en) Ink jet device and method for releasing a plurality of substances onto a substrate
WO2007060634A1 (fr) Dispositif de projection d&#39;encre qui libere de maniere controlee plusieurs substances sur un substrat, procede permettant de distinguer differentes substances et utilisation du dispositif de projection d&#39;encre
JP3701594B2 (ja) 液滴吐出方法
US20040020942A1 (en) Device and system for dispensing or aspirating/dispensing liquid samples
WO2002092228A2 (fr) Procede et dispositif de distribution de gouttelettes
JP2002204945A (ja) マイクロピペット、分注装置及びバイオチップの製造方法
Perçin et al. Micromachined piezoelectrically actuated flextensional transducers for high resolution printing and imaging
US10112212B1 (en) Droplet ejection using focused acoustic radiation having a plurality of frequency ranges
EP3426408A1 (fr) Vibration d&#39;une tête de distribution pour déplacer un fluide
WO2003101369A2 (fr) Dispositif de transfert de liquides
US20040035948A1 (en) Liquid transfer device
Perçin et al. Micromachined fluid ejector arrays for biotechnological and biomedical applications
Perçin et al. Micromachined Piezoelectrically Actuated Flextensional Transducers For High Resolution Printing

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
COP Corrected version of pamphlet

Free format text: PAGES 1/3-3/3,DRAWINGS,REPLACED BY NEW PAGES BEARING THE SAME NUMBER;DUE TO LATE TRANSMITTAL BY THERECEIVING OFFICE

ENP Entry into the national phase

Ref country code: JP

Ref document number: 1997 516786

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1996936933

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1996936933

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1996936933

Country of ref document: EP