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WO1997030784A1 - Microreacteur - Google Patents

Microreacteur Download PDF

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Publication number
WO1997030784A1
WO1997030784A1 PCT/GB1997/000496 GB9700496W WO9730784A1 WO 1997030784 A1 WO1997030784 A1 WO 1997030784A1 GB 9700496 W GB9700496 W GB 9700496W WO 9730784 A1 WO9730784 A1 WO 9730784A1
Authority
WO
WIPO (PCT)
Prior art keywords
microreactor
visual identification
container
microreactors
synthesis
Prior art date
Application number
PCT/GB1997/000496
Other languages
English (en)
Inventor
David Gani
Mahmoud Akhtar
Friedrich Erich Karl Kroll
Colin Forbes Macdonald Smith
Original Assignee
The University Court Of The University Of St. Andrews
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Court Of The University Of St. Andrews filed Critical The University Court Of The University Of St. Andrews
Priority to JP9529908A priority Critical patent/JP2000506127A/ja
Priority to AU18865/97A priority patent/AU1886597A/en
Priority to EP97905242A priority patent/EP0881944A1/fr
Publication of WO1997030784A1 publication Critical patent/WO1997030784A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00295—Individual reactor vessels the reactor vessels having pervious side walls
    • B01J2219/00299—Generally cylindrical reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00461—Beads and reaction vessel together
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00542—Alphanumeric characters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00545—Colours
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00547—Bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00592—Split-and-pool, mix-and-divide processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Definitions

  • the invention relates to a microreactor, and especially a microreactor for synthesising chemical compounds.
  • reaction chemistry needs to be irreversible. such that each of the starting materials in the mixture is converted to a new product.
  • solid-phase synthesis the advantages of solid-phase synthesis are that the products do not need to be purified by, for example, solvent extraction, distillation, recrystallisation or chromatography but rather are retained on the solid medium by washing away the excess reagents and impurities.
  • SPS solid- phase synthesis
  • the third problem concerns the deconvolution of the library which essentially requires identifying the chemical structure of the molecule, within the mixture, that shows the required biological activity or other desired property.
  • This method of deconvolution is time consuming and unnecessarily clumsy.
  • Another way of effecting deconvolution is to tag the polymeric support with chemicals which can be used to decode the synthetic chemical history of the particular particle of polymeric support, independently to being able to carry out an activity assay on the material attached to the support.
  • Sue i methods have been described in the literature. Since typical particles of polymeric support are referred to as "resin beads" and are commercially available in the size 90-400 microns, deconvolution by such methods is a fiddly job requiring accurate and expensive instrumentation.
  • the fourth problem concerns checking the efficiency of the chemical synthesis and, in essence, this is a problem of scale.
  • Individual beads possess, at most, only a few nanomoles of material attached to them and thus it is extremely difficult to check either the efficiency of the synthesis or the purity of the synthetic product. In highly sensitive biological screening assays this can be a very serious problem as the impurity could be responsible for a positive result.
  • the best way to overcome this last problem is to perform syntheses on a larger scale such that some material can be put aside for characterisation and analysis. While this solution offers very many advantages, the practice of larger scale combinatorial syntheses requires the design and use of microreactors.
  • a microreactor for synthesis of chemical compounds comprises a container comprising a body section; entry means to permit fluid to enter the container; and a visual identification device to enable visual identification of the microreactor.
  • microreactor means a container comprising a material which is permeable to fluids .
  • the container may enclose a solid material or particles on or with which reaction occurs, and the container is impermeable to the solid material or particles.
  • the material of the container itself may comprise a chemically functionalised polymer on or with which reaction occurs. This can be referred to as a "bonded" microreactor.
  • the microreactor may further comprise a closure and the body section may have an opening, the closure being adapted to close the opening, and fluid being able to enter the container through the entry means when the opening is closed by the closure.
  • the body section may comprise a material which comprises a polymeric support on or with which reaction occurs.
  • the polymeric support may be chemically functionalised polystyrene and may be in the form of a porus, frit or sintered material.
  • a method of identifying a microreactor for synthesis of chemical compounds comprises attaching a visual identification device to the microreactor to enable the microreactor to be visually identified.
  • An advantage of the invention is that it permits deconvolution of a library of synthesised molecules by visual identification of a microreactor.
  • the visual identification device may comprise a character and/or a colour.
  • the character may be an alphanumeric character.
  • the visual identification device may be attached to the external surface of the container.
  • the visual identification device may be inserted into the container or may be incorporated into the material of the container, which may be the body section and/or the closure.
  • the closure may be removable or non-removable from the opening.
  • each microreactor may comprise a number of visual identification devices, which may be different or identical.
  • the visual identification devices may be attached to the microreactor prior to the microreactor being used for synthesis of chemical compounds. Alternatively, the visual identification devices may be attached as appropriate before or after each stage in the synthesis procedure, one at a time or several at a time.
  • the visual identification device may be of a size to be visually identified by humans, or alternatively may be identified by robotics or another type of machine.
  • a separate visual identification device is provided for each chemical in which the microreactor is immersed during synthesis.
  • the body section may have two openings and two removable closures, one closure for each opening.
  • the body section may be tubular with the openings provided at each end of the tubular body section.
  • the body section which may be cylindrical in form.
  • bonded microreactors which themselves consist of chemically functionalised frit glass or frit or foamed polymer, there do not need to be openings for loading and unloading of resin, as the chemically reactive groups would be retained within the bonded matrix itself.
  • the device may comprise a ring shaped member which is fitted over the body section and visual identification may be provided by a colour of the member and/or by characters on the surface of the member.
  • the visual identification device may be inserted into holes or apertures in a side wall of the container.
  • the visual identification device may comprise a peg or bead which fits into and is held in the hole or aperture.
  • the entry means is provided by apertures in the side walls of the container.
  • the side walls may comprise frit material, a perforated polymer material or a mesh. It is possible that a combination of these materials could be used. Examples of suitable frit materials are frit glass, frit polyethylene, frit polypropylene and frit polytetrafluoroethylene (PTFE) .
  • the closure may be attached to the body section by being a push fit into the opening, by being threadedly connected to the body section or attached by an adhesive.
  • the microreactors may have a length of approximately 7- 10mm, and internal width of 3.5-7mm and an outside width of 4-lOmm.
  • the microreactors are for use with standard commercially available polymer beads of 90-400 microns for solid support in the solid phase synthesis.
  • microreactors or smaller microreactors may be constructed for other applications.
  • larger microreactors may be constructed and used for non-biological applications.
  • the microreactor and the visual identification device are composed of cheap inert material and the selection of the materials is dictated by the intended chemistry, ie only compatible materials are used, eg glass is not used with aqueous hydrofluoric acid and non-resistant polymers are not used with organic solvents.
  • Fig. 1 is a cross sectional view through a first example of a microreactor
  • Fig. 2 is a cross sectional view through a second example of a microreactor
  • Fig. 3 is a perspective view of a third example of a microreactor
  • Fig. 4 is a plan view of the microreactor shown in Fig. 3
  • Fig. 5 is a front view of the microreactor shown in Fig. 3
  • Fig. 6 is a back view of the microreactor shown in Fig. 3
  • Fig. 7 is an exploded side view of a fourth example of a microreactor
  • Fig. 8 is a side view of the microreactor of Fig. 7 assembled
  • Fig. 9 is a flow diagram illustrating how twenty- seven microreactors may be used to synthesise twenty-seven compounds from three suitably functionalised starting compounds.
  • Fig. 1 shows a first example of a microreactor 1 which comprises a polymer tube having 70 micron perforations in the wall of the tube 2. At each end of the tube 2 is an end cap 3. The material from which the tube 2 and end caps 3 are manufactured is inert with the compounds into which the microreactor 1 is to be immersed. Located within the microreactor 1 are a number of polymer beads 4 for solid support in solid phase synthesis. The polymer beads have a diameter which is greater than 70 microns.
  • FIG. 2 A second example of a microreactor 5 is shown in Fig. 2.
  • the microreactor 5 comprises a container body section 6 having an open end 7 which is closed by a removable lid 8.
  • the container body section 6 and the removable lid 8 are both manufactured from frit glass and the frit glass is chosen to be inert with the compounds in which the microreactor 5 is to be immersed. However, any other suitable frit material may be used.
  • the microreactor 5 also contains a number of polymer beads for solid support in solid phase synthesis.
  • Figs. 3 to 6 show a third example of a microreactor 20 which is manufactured from a frit material.
  • a frit material This may be frit glass, frit polyethylene, frit polytetrafluoroethylene or any other suitable frit material.
  • a "suitable frit material” is any frit material which is inert with the chemicals into which the microreactor 20 is to be immersed.
  • the microreactor 20 consists of a cylindrical body section 21 which has a hole 22 drilled into the curved surface of the cylindrical body section 21. Hole 22 has polymer beads inserted into it before the hole 22 is plugged by a plug 23. Around the curved surface of the body section 21 a number of small holes 24 are drilled. These holes permit small coloured pegs to be attached to the microreactor 20 by being pushed into the holes 24.
  • the plugged hole 22 forms a reaction chamber into which chemical fluids may enter through the holes in the frit material from which the body section 21 is formed.
  • the plug 23 may be any suitable inert material, such as an inert polymer.
  • the microreactor could be manufactured from porous or frit perfluoroalkyl sulphonic acid resin, such as Nafion (trade mark) manufactured by Du Pont, so that the material of the microreactor itself forms the polymeric support.
  • porous or frit perfluoroalkyl sulphonic acid resin such as Nafion (trade mark) manufactured by Du Pont
  • other chemically functionalised sintered, frit or porous polymers or composites could be used to form the microreactor.
  • the microreactor would not have the reaction chamber 22 or the closure 23 and would be a body of material porous or frit material. However, the holes 24 for the coloured pegs would still be present. The reactions then take place on or with the material of the microreactor itself.
  • Figs. 7 and 8 show a fourth example of a microreactor 30.
  • Fig. 7 is an exploded side view of the microreactor 30 showing the components of the microreactor 30.
  • the microreactor 30 has a tubular glass body 31 which has an external screw thread formation 32.
  • the body 31 is hollow and two sealing rings 33 and a frit glass end closure 34 are secured to each end of the glass body 31 by an end cap 35.
  • the end caps 35 are internally threaded so that they screw onto the thread 32 on the body 31.
  • end closures 34 are of a frit material, such as a plastic, it would not be necessary to use the sealing rings 33.
  • one end cap 35, end closure 34 and sealing rings 33 are secured to one end of the body 31.
  • the polymer beads may then be placed in the body 31 through the other open end. The open end is then closed using the other end cap 35, end closure 34 and sealing rings 33.
  • the visual identification devices for the microreactor 30 may be moulded into the end caps 35, which may be moulded from a plastics material.
  • the end caps 35 and/or body 31 may be individually colour coded.
  • the body 31 is solid glass and not frit glass .
  • Fig. 8 shows the assembled microreactor 30.
  • the fluids enter the microreactor through the end closures 34 which are of a frit material, and therefore permeable to fluids but not to the polymer beads placed inside the microreactor 30.
  • microreactors Both frit glass tubes and rectangular chambers and perforated polymer tubes and meshes with appropriate lids were used as microreactors in the synthesis of small peptide libraries.
  • Standard commercially available polymer beads 4 of 90-400 microns were used for the solid support in the solid phase synthesis (SPS).
  • SPS solid phase synthesis
  • the dimensions of the microreactors range from a length of 7-lOmm, with an internal diameter of 3.5-7mm, and an outside diameter of 4-10mm, depending on the material.
  • the walls of frit glass tube need to be thicker to provide mechanical strength.
  • the lids 3, 8 of the microreactors 1, 5 and the plug 23 of the microreactor 20 are resistant polymer or frit glass and can be colour coded as part of the visually addressable system.
  • the microreactors 1, 5, 20 themselves can also be colour coded or marked with the appropriate alpha- numeric or icon, or with multiple visual identifications. Larger microreactors can be constructed for non-biological applications using the same
  • microreactors used were pre-labelled, that is the colours and alpha-numerics were already associated with each of the microreactors such that the chemical synthesis was programmed by the visual identification marks.
  • this method offers no advantages or disadvantages in identification compared with tagging the microreactors after each cycle of the synthesis.
  • pre-labelled microreactors could be used in programmed robotic synthesis, where the machine or human readable identification is used to determine which vessel the microreactor is placed into for the next step.
  • microreactors could be manufactured and supplied in a coded form for the user to predetermine what each element of the code will mean in the synthesis of the chemical libraries. This also saves the user from needing to tag the microreactor after each step.
  • microreactors have the potential to be smaller than those described above where for human visualisation, as opposed to robotic identification, the microreactor is read using a magnifying glass, typically of the type used by electronics engineers for identifying resistors and chips etc.
  • the limit of the number of sets of colours, alphanumerics, etc that can be read easily on the microreactors described above is six, without the aid of a magnifying glass. This number could be increased to twelve by precision manufacture of the microreactors for visual identification using a magnifying glass. However, in practise, twelve represents the number of actual synthetic steps (not counting chemical activation and protection and deprotection steps which support the synthetic chemistry) and twelve is probably beyond the need of any potential application other than bioactive peptide synthesis.
  • the structural (molecular) diversity is limited by the visualisation method. For example, there are ten easily distinguishable colours and if all ten are used for each of six syntheses steps there are 10 6 individually addressable microreactors.
  • a small amount of pre-swollen commercially available resin for solid phase peptide synthesis is added to the pre- labelled microreactor as a slurry in dimethylformamide such that the microreactor is half-full or less.
  • a small glass bead or stirring magnet may be added to ensure thorough mixing.
  • the microreactor, and any others which are to be processed, are placed in the main reaction vessel and are drowned in a solution of solvent eg dimethylformamide containing the appropriate reagents for either synthesis or deprotection in the usual way.
  • the microreactors are physically agitated to ensure that each resin bead is exposed to the reagent solution.
  • microreactors are then transferred to new appropriate reaction vessels, together with other microreactors, as dictated by the visually addressable labels for further cycles of deprotection of synthesis. The entire process is repeated until the synthesis and deprotection is complete.
  • the library of labelled microreactors is now ready for solid phase assay (on the polymer bead) where individual beads are removed to prepare a library or sub-library of beads of known composition. If solution phase assays are to be performed, the compounds are obtained by un-linking the polymer resin support. This can be performed either on the entire contents of any or all of the individual microreactors, or on just a portion of the contents. Unlinking is performed in the usual way.
  • bonded microreactors composed of porous functionalised polymer, for example, perfluoroalkyl sulphonic acid or carboxylic acid resins such as Nafion or those manufactured by Asahi or Dow
  • the acid groups would be activated to load appropriate nucleophilic linker groups, for example, 3-aminobenzyl alcohol to give a chemical reaction surface similar to that for commercially available resins.
  • POSAM permutational organic synthesis in addressable microreactors
  • microreactors 11 containing compound A, three containing B and three containing C are then reacted with compound A.
  • the microreactors 11 are labelled with a further visually identifiable tag.
  • Nine further microreactors 11 containing A, B and C (three of each) are then reacted with compound B and then tagged and the remaining nine microreactors 11 containing A, B and C are then reacted with compound C and then tagged.
  • one set of the nine compounds is reacted with compound A, and then tagged and a further set of nine reacted with compound B, and then differentially tagged and finally, the last set of nine compounds is reacted with compound C and then tagged.
  • the visual identification of microreactors also ensures that no mistakes are made during various cycles of library synthesis and avoids the statistical problems generated in the split and mix strategy that is used when dealing directly with the indistinguishable polymeric beads. If the synthetic efficiency of the chemical process needs to be interrogated, it is either possible to open up a microreactor and remove some of the material for analysis or to include extra identical microreactors visually tagged in the appropriate manner which are removed during the synthetic procedure, specifically for analysis.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un microréacteur (20) pour la synthèse de composés chimiques comprend un récipient ayant une portion de corps (21). Des pores d'entrée permettent au fluide d'entrer dans le récipient et un dispositif d'identification visuelle permet une identification visuelle du microréacteur (20).
PCT/GB1997/000496 1996-02-24 1997-02-24 Microreacteur WO1997030784A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP9529908A JP2000506127A (ja) 1996-02-24 1997-02-24 マイクロリアクタ
AU18865/97A AU1886597A (en) 1996-02-24 1997-02-24 A microreactor
EP97905242A EP0881944A1 (fr) 1996-02-24 1997-02-24 Microreacteur

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9603945.8 1996-02-24
GBGB9603945.8A GB9603945D0 (en) 1996-02-24 1996-02-24 A microreactor

Publications (1)

Publication Number Publication Date
WO1997030784A1 true WO1997030784A1 (fr) 1997-08-28

Family

ID=10789346

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1997/000496 WO1997030784A1 (fr) 1996-02-24 1997-02-24 Microreacteur

Country Status (5)

Country Link
EP (1) EP0881944A1 (fr)
JP (1) JP2000506127A (fr)
AU (1) AU1886597A (fr)
GB (1) GB9603945D0 (fr)
WO (1) WO1997030784A1 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1006813C1 (nl) * 1997-08-20 1998-01-21 Sipke Wadman Verpakkingen voor een vast reactie-draagmedium.
WO1999064158A1 (fr) * 1998-06-10 1999-12-16 Graffinity Pharmaceutical Design Gmbh Support de reacteur a plusieurs chambres de reception de microechantillons
WO2000000455A1 (fr) * 1998-06-27 2000-01-06 The University Court Of The University Of St Andrews Composes d'ether perfluorovinyle et resines
US6342185B1 (en) 1999-12-15 2002-01-29 Uop Llc Combinatorial catalytic reactor
US6368865B1 (en) 1999-12-15 2002-04-09 Uop Llc Combinatorial process for performing catalytic chemical reactions
JP2002542927A (ja) * 1999-04-28 2002-12-17 アベシア・リミテッド 反応器
US6576196B1 (en) 1999-12-15 2003-06-10 Uop Llc Multiple parallel catalytic reactor assembly
US6627445B1 (en) 1999-12-15 2003-09-30 Uop Llc Process for simultaneously evaluating a plurality of catalysts
US6770245B2 (en) 1999-12-15 2004-08-03 Uop Llc Multiple parallel processing assembly
US6776963B1 (en) 1999-12-15 2004-08-17 Uop Llc Multiple parallel catalytic reactor assembly
US6808685B2 (en) 2001-09-17 2004-10-26 Uop Llc Apparatus and method for generating a plurality of isolated effluents
EP1252126A4 (fr) * 2000-02-03 2006-07-05 Nanoscale Combinatorial Synthe Synthese non redondante partage/melange de bibliotheques combinatoires
US7141217B2 (en) 2002-12-05 2006-11-28 Uop Llc Elevated pressure apparatus and method for generating a plurality of isolated effluents
DE102008023545A1 (de) * 2008-05-14 2009-11-19 Roderfeld Und Bora Gmbh & Co.Kg Vorrichtung zur Herstellung einer biologisch aktiven Substanz
US11192076B2 (en) * 2017-03-06 2021-12-07 Spinchem Ab Flow-promoting device, a reactor arrangement and the use of such flow-promoting device
US11633709B2 (en) 2017-05-23 2023-04-25 Microwave Chemical Co., Ltd. Treatment apparatus

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JP6311906B1 (ja) * 2017-05-23 2018-04-18 マイクロ波化学株式会社 処理装置

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Cited By (21)

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NL1006813C1 (nl) * 1997-08-20 1998-01-21 Sipke Wadman Verpakkingen voor een vast reactie-draagmedium.
WO1999064158A1 (fr) * 1998-06-10 1999-12-16 Graffinity Pharmaceutical Design Gmbh Support de reacteur a plusieurs chambres de reception de microechantillons
WO2000000455A1 (fr) * 1998-06-27 2000-01-06 The University Court Of The University Of St Andrews Composes d'ether perfluorovinyle et resines
JP2002542927A (ja) * 1999-04-28 2002-12-17 アベシア・リミテッド 反応器
US6342185B1 (en) 1999-12-15 2002-01-29 Uop Llc Combinatorial catalytic reactor
US6368865B1 (en) 1999-12-15 2002-04-09 Uop Llc Combinatorial process for performing catalytic chemical reactions
US6576196B1 (en) 1999-12-15 2003-06-10 Uop Llc Multiple parallel catalytic reactor assembly
US6627445B1 (en) 1999-12-15 2003-09-30 Uop Llc Process for simultaneously evaluating a plurality of catalysts
US6770245B2 (en) 1999-12-15 2004-08-03 Uop Llc Multiple parallel processing assembly
US6776963B1 (en) 1999-12-15 2004-08-17 Uop Llc Multiple parallel catalytic reactor assembly
US7288411B2 (en) 1999-12-15 2007-10-30 Uop Llc Process for simultaneously evaluating a plurality of catalysts
EP1252126A4 (fr) * 2000-02-03 2006-07-05 Nanoscale Combinatorial Synthe Synthese non redondante partage/melange de bibliotheques combinatoires
US6808685B2 (en) 2001-09-17 2004-10-26 Uop Llc Apparatus and method for generating a plurality of isolated effluents
US7129092B2 (en) 2001-09-17 2006-10-31 Uop Llc Apparatus and method for generating a plurality of isolated effluents
US7070999B1 (en) 2001-09-17 2006-07-04 Uop Llc Apparatus and method for generating a plurality of isolated effluents
US7141217B2 (en) 2002-12-05 2006-11-28 Uop Llc Elevated pressure apparatus and method for generating a plurality of isolated effluents
US7256044B2 (en) 2002-12-05 2007-08-14 Uop Llc Elevated pressure apparatus and method for generating a plurality of isolated effluents
DE102008023545A1 (de) * 2008-05-14 2009-11-19 Roderfeld Und Bora Gmbh & Co.Kg Vorrichtung zur Herstellung einer biologisch aktiven Substanz
US11192076B2 (en) * 2017-03-06 2021-12-07 Spinchem Ab Flow-promoting device, a reactor arrangement and the use of such flow-promoting device
US11633709B2 (en) 2017-05-23 2023-04-25 Microwave Chemical Co., Ltd. Treatment apparatus
US12290792B2 (en) 2017-05-23 2025-05-06 Microwave Chemical Co., Ltd. Treatment apparatus

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JP2000506127A (ja) 2000-05-23
AU1886597A (en) 1997-09-10
GB9603945D0 (en) 1996-04-24

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