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WO1998004725A1 - Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues - Google Patents

Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues Download PDF

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Publication number
WO1998004725A1
WO1998004725A1 PCT/US1997/013358 US9713358W WO9804725A1 WO 1998004725 A1 WO1998004725 A1 WO 1998004725A1 US 9713358 W US9713358 W US 9713358W WO 9804725 A1 WO9804725 A1 WO 9804725A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
altered
amount
dna
methylated dna
Prior art date
Application number
PCT/US1997/013358
Other languages
English (en)
Inventor
Stephen L. Dellaporta
Jychian Chen
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Priority to AU40480/97A priority Critical patent/AU730644B2/en
Priority to EP97938066A priority patent/EP0935666A1/fr
Priority to CA002262779A priority patent/CA2262779A1/fr
Priority claimed from US08/902,902 external-priority patent/US6011200A/en
Publication of WO1998004725A1 publication Critical patent/WO1998004725A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • Plant genomes contain relatively large amounts of the modified nucleotide
  • ddm mutants exhibit a slight delay (1.7 days) in flowering, altered
  • a reduction in the rate a plant matures can be used to increase the biomass
  • the present invention is based on the unexpected observation that a decrease
  • DNA present in a plant genome results in a plant that requires less time to mature.
  • the rate of maturation is increased by altering the plant
  • plant cells plant tissues or plant seeds, using molecular techniques, such that the plant
  • the rate of maturation is decreased by altering a plant, plant cells, plant tissues or plant seeds, using molecular
  • the present invention further provides plants that have an altered rate of
  • Figure 1 The predicted MET1 gene product and antisense construct.
  • the METJ protein is a 1,534-amino acid protein with a high degree
  • MET! antisense construct is shown in the bottom of the figure. See Example 1.
  • mutants were digested with Hpa II (left panels) or Msp I (right panels), subjected to
  • LEAFY LFY
  • Tr246 to wild-type Arabidopsis Cold-type Arabidopsis (Columbia strain with no mutations) were digested
  • the present invention is based on the unexpected observation that DNA
  • methylation particularly at cytosine nucleotides, is involved in regulating the rate of
  • the present invention is based on the
  • the present invention provides methods for
  • maturation refers to the process of plant differentiation leading
  • the method will produce plants with an increased
  • Arabidopsis plants were obtained which developed flowers after 10 to 12 days.
  • the result of accelerating plant development is the ability to decrease the
  • biomass refers to the total plant weight, particularly leafy material. The amount of leaf material is increased in plants altered
  • Another consequence of delaying flower production is to produce plants that
  • plants propagated containing the genetic alteration can be used in the present method. Methods known in the art for molecularly altering a plant are discussed in detail
  • the preferred plants include both dicot and monocotyledonous plants.
  • most preferred plants are plants with economic value as a food or biomass source, or
  • plants with long maturation times Such plants include, but are not limited to, leafy
  • plants such as tobacco, spinach, lettuce, and seed bearing plants such as rice, corn, soy
  • the methods of the present invention rely on altering plants using molecular
  • non-molecular methods namely selecting plants generated through methods such as
  • plants and plant cells can be subjected to chemical mutagenesis, physical mutagenesis
  • Plants can then be
  • the methods of the present invention can be used to alter
  • a plant is said to have a reduced or decreased amount of
  • methylated DNA when the plant has less methylated DNA than the non-altered plant.
  • cytosine has been seen to be methylated in plants.
  • present methods are therefore accomplished by targeting the methylation of cytosine.
  • cytosine were obtained.
  • targets, strategies and molecular techniques can be used by a variety of targets, strategies and molecular techniques.
  • methyl transferases that are normally produced in the plant.
  • the methyl transferase can be altered in a variety of ways by a skilled artisan
  • Any of a plant's DNA methyl transferases genes can be used as a target in the
  • the most preferred targets are the genes encoding a cytosine methyl
  • DNA methyl transferase genes examples include
  • cytosine methyltransferase such as the MET1 Arabidopsis gene herein used, the
  • plant is to create knockout mutants in the plant in which one or more of the plant's
  • DNA methyl transferase genes are inactivated. This can be accomplished through the
  • transposon mediated mutagenesis can be used for the same purpose.
  • transferase gene allowing one to activate or deactivate methyl transferase expression
  • the methods of the present invention can be used to alter
  • a plant is said to have a reduced or decreased amount of
  • methylated DNA when the plant has less methylated DNA than the non-altered plant.
  • cytosine has been seen to be methylated in plants.
  • present methods are therefore accomplished by targeting the methylation of cytosine.
  • methyl transferase in the plant cell.
  • the methyl transferase can be altered in a variety of ways by a
  • DNA methyl transferase encoding expression units can be any suitable DNA methyl transferase encoding expression units.
  • DNA methyl transferase encoding expression units can be any suitable DNA methyl transferase encoding expression units.
  • expression units can be controlled either by a constitutive promoter, providing
  • Any DNA methyl transferase gene, or active fragment thereof, can be used to generate a stable copy of a gene.
  • DNA can be further altered so as to contain an expression unit that expresses a DNA
  • plants are of additional value because of the ability to induce expression of a DNA
  • the present invention further provides plants that have been altered using
  • the plants of the present invention fall within two types.
  • the first type is a first type
  • plants of the present invention that have a slower rate of maturation will mature at a
  • plants or the present invention include those that have been altered, using
  • the preferred plants are plants for which it is desirable to reduce generation times for breeding or seed production
  • expression units or expression vectors or systems to express an exogenously
  • a supplied gene such as a DNA methyl transferase, or antisense molecule, in a plant.
  • methylated DNA present in a plant cell typically employ a protein or
  • antisense coding region such as the METl Arabidopsis gene, or a homologue thereof
  • antisense coding region can either be the expression control element that is
  • Transcription initiation regions can include any of the various opine
  • initiation regions such as octopine, mannopine, nopaline and the like that are found in
  • plant promoters such as prolifera promoter,
  • promoters etc. can also be used. The most preferred promoters will be active in
  • tissue particularly meristematic cells.
  • Either a constitutive promoter such as the CaMV or Nos promoter illustrated above, an organ-specific promoter (such as the E8 promoter from tomato) or an
  • inducible promoter is typically ligated to the protein or antisense encoding region
  • the expression unit may be further
  • the expression units will typically contain, in
  • restriction enzyme sites at the 5' and 3' ends of the expression unit are typically
  • the promoter is
  • the expression cassette can also contain a promoter sequence
  • the termination region may be obtained from the same gene as
  • the promoter sequence or may be obtained from different genes. If the mRNA
  • Polyadenylation sequences include, but are not limited to the Agrobacterium octopine
  • the resulting expression unit is ligated into or otherwise constructed to be included in a vector which is appropriate for higher plant transformation.
  • the vector will also typically contain a selectable marker gene by which transformed plant cells
  • the marker gene can be identified in culture.
  • the marker gene will encode antibiotic
  • markers include resistance to G418, hygromycin, bleomycin,
  • vector will be identified by their ability to grow on a medium containing the particular
  • Replication sequences of bacterial or viral origin, are generally also
  • a vector included to allow the vector to be cloned in a bacterial or phage host, preferably a
  • bacteria should also be included to allow selection of bacterial cells bearing the
  • Suitable prokaryotic selectable markers also include resistance to
  • antibiotics such as kanamycin or tetracycline.
  • T-DNA sequences will also be included for subsequent transfer to
  • plants having an altered amount of methylated DNA can be produced by:
  • a plant may also be used in the present methods. For example, formation of a triple
  • photocrosslinking is described, e.g., in D. Praseuth, et al, Proc. Nat 'I Acad. Sci. USA
  • transgenic plants are prepared which contain the desired expression unit or into which
  • the vector is microinjected directly into plant cells by use of
  • micropipettes to mechanically transfer the recombinant DNA into the plant cell
  • nucleic acid either within the matrix of small beads or particles, or on the
  • These entities are minicells, cells, lysosomes or other fusible lipid-surfaced
  • DNA may also be introduced into the plant cells by electroporation (From et
  • the cell wall divide, and regenerate.
  • a plant cell For transformation mediated by bacterial infection, a plant cell is infected with
  • Agrobacterium is a representative genus of the gram-negative family
  • Rhizobiaceae Its species are responsible for crown gall (A. tumefaciens) and hairy
  • the bacterial genes responsible for expression of opines are a
  • assaying for the presence of opines can be used to identify transformed tissue.
  • Heterologous genetic sequences can be introduced into appropriate plant cells,
  • Ti or Ri plasmid is transmitted to plant cells on infection by Agrobacterium and is
  • Ti and Ri plasmids contain two regions essential for the production of transformed
  • T-DNA transferred DNA
  • the other termed the virulence (yir) region, is
  • the T-DNA will be any substance that is essential for the transfer of the T-DNA but is not itself transferred.
  • the T-DNA will be any substance that is not be transferred.
  • the transferred DNA region can be transferred into a plant cell even if the yji region is on a different plasmid (Hoekema, et al, Nature 303: 179-189 (1983)).
  • the transferred DNA region can be
  • plasmids and sometimes constructed from foreign sequences. These may include but
  • co-integrate the shuttle vector containing the gene of
  • the gene of interest is inserted into a shuttle vector containing the cz ' -v-acting elements required for plant transformation.
  • the other necessary functions are
  • the second method requires (a) that the intact plant tissues, such as
  • cotyledons can be transformed by Agrobacterium and (b) that the transformed cells
  • Plant cells which have been transformed can also be regenerated using known
  • Means for regeneration vary from species to species of plants, but generally a suspension of transformed protoplasts or a petri plate containing transformed explants
  • Callus tissue is formed and shoots may be induced from callus and
  • somatic embryo formation can be induced in the
  • the culture media will generally contain various amino acids and plant hormones, such as auxin and cytokinins. It is also advantageous to add glutamic acid and proline
  • silencing inactivation of transgenes is known as silencing.
  • T-DNA vector pMON530 (Monsanto) in the antisense orientation to the CaMV 35S
  • T-DNA confers resistance to kanamycin (50 ⁇ g/mi) on transgenic plants.
  • PROLIFERA PRL
  • CONSTITUTIVELY PHOTOMORPHOGENIC 1 CO
  • ERECTA ER
  • Tr246 to wild-type Arabidopsis Cold-type Arabidopsis (Columbia strain with no mutations) were digested
  • the METl gene represents one member of a
  • METl gene is expressed in seedling, vegetative, and floral tissues; in the
  • T-DNA single-locus transferred DNA
  • g/1 locus as a marker (but otherwise wild type), except as noted by Agrobacterium-
  • the plants used in this study represent the kanamycin-resistant progeny of TI outcrosses from single-locus lines Tr242, 243, 244, 245, and 248.
  • Seedlings were transplanted into soil 8 days after germination.
  • Genomic DNAs were digested with the isochizomers Hpa II or Msp I (Fig.
  • Msp I can cleave C 5m CGC but not 5m CCGG (M. Nelson, et ⁇ l, Nucleic
  • genomic DNAs were isolated from plants immediately after the onset of flowering,
  • Spherical Microsorb C18 column (100 A pore size, 4.5 mm inner diameter by 15 cm length) with a 20 min. isocratic gradient of 2.5% methanol, 50 mM KH,PO 5 (pH 4.0),
  • Tr248 was reduced 71% relative to wild-type levels; the weak line Tr245 showed a
  • antisense lines was determined by reversed-phase HPLC using the method of Gehrke
  • Tr248 1.83 ⁇ 0.16 28.7 71.3 ddml 1.60 ⁇ 0.04 25.0 75.0
  • Flowering time refers to the number of days elapsed from seed germination until emergence of an inflorescence bolt 0.5 to 1.0 cm
  • leaf number refers to the number of vegetative leaves initiated before
  • Tr242 24.5 ⁇ 2.1 10.0 ⁇ 1.4 4.5 ⁇ 1.1
  • Tr248 46.3 ⁇ 4.7 32.9 ⁇ 2.7 20.5 ⁇ 2.0
  • Tr242, 243, 244, 245, and 248 were plated on MS
  • Tr244, Tr246 and Tr248 plants initiated 30 to 35 vegetative nodes with delayed abaxial trichome production and flowered after 45 to 48 days
  • vegetative rosettes including enhanced spiral phyllotaxy of 10 to 20 vegetative-like
  • tertiary inflorescences showed gross abnormalities, including a threefold increase in
  • MTase differs markedly from the phenotype produced by the treatment of Arabidopsis
  • 5-AzaC produces early flowering in some late-flowering strains and mutants of Arabidopsis (J. E. Burn, et al, Proc.
  • Methylation may serve as a primary signal to restrict meristem determinacy, or it may
  • phase transitions are often irregular in strong antisense plan — the location of
  • branches apical to flowers in the inflorescence transition zone may represent an
  • plants were altered to contain an expression unit that expresses cytosine methyl transferase under the control of the CaMV 355 promoter or another plant promoter.
  • the expression units were introduced into a plant using Agrobacterium as
  • plants having an increased amount of methylated DNA produced flowers at 10 to 12
  • the present methods can be applied to any plant that can be altered using
  • the first step in applying the present methods is to select as
  • the plant is altered so as to contain an increased amount of methylated DNA while to
  • the second step may be to decide which method to employ to alter the plant.
  • the amount of DNA methylation can be decreased by inactivating
  • methylation can be increased by activating or increasing the methylase activity within

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention est basée sur l'observation inattendue que la méthylation d'ADN, notamment au niveau des restes cytosine, régule la vitesse de croissance d'une plante. D'après cette observation, l'invention concerne des procédés consistant à augmenter ou diminuer la vitesse de croissance d'une plante, à l'aide d'une augmentation ou d'une diminution de la quantité d'ADN méthylé trouvé dans la plante. La présente invention concerne en outre des plantes que l'on a ainsi modifiées afin d'augmenter ou de diminuer leur vitesse de maturation par rapport à celle d'une plante non modifiée.
PCT/US1997/013358 1996-07-31 1997-07-30 Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues WO1998004725A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU40480/97A AU730644B2 (en) 1996-07-31 1997-07-30 Methods for altering the rate of plant development and plants obtained therefrom
EP97938066A EP0935666A1 (fr) 1996-07-31 1997-07-30 Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues
CA002262779A CA2262779A1 (fr) 1996-07-31 1997-07-30 Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2331496P 1996-07-31 1996-07-31
US60/023,314 1996-07-31
US08/902,902 US6011200A (en) 1997-07-30 1997-07-30 Methods for altering the rate of plant development and plants obtained therefrom

Publications (1)

Publication Number Publication Date
WO1998004725A1 true WO1998004725A1 (fr) 1998-02-05

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055891A1 (fr) * 1998-04-30 1999-11-04 Washington University Gene vegetal regulant la methylation de l'adn
WO2001000801A3 (fr) * 1999-06-23 2001-05-10 Syngenta Participations Ag Gene intervenant dans l'inhibition epigenetique d'un gene
WO2001009299A3 (fr) * 1999-07-30 2001-08-23 Univ Bath Plantes modifiees
EP1082417A4 (fr) * 1998-06-04 2006-08-23 Univ Queensland Sequences genetiques modifiant le phenotype
US7125857B2 (en) 1997-08-29 2006-10-24 The Regents Of The University Of California Modulators of DNA cytosine-5 methyltransferase and methods for use thereof
WO2005038040A3 (fr) * 2003-10-14 2006-11-09 Ceres Inc Procedes et compositions permettant de modifier les phenotypes des semences
US7429692B2 (en) 2004-10-14 2008-09-30 Ceres, Inc. Sucrose synthase 3 promoter from rice and uses thereof

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO1996014414A1 (fr) * 1994-11-02 1996-05-17 John Innes Centre Innovations Limited Regulation genetique du fleurissement
DE4444460A1 (de) * 1994-11-29 1996-05-30 Inst Genbiologische Forschung Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen

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Publication number Priority date Publication date Assignee Title
WO1996014414A1 (fr) * 1994-11-02 1996-05-17 John Innes Centre Innovations Limited Regulation genetique du fleurissement
DE4444460A1 (de) * 1994-11-29 1996-05-30 Inst Genbiologische Forschung Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
FINNEGAN, E.J., ET AL .: "ISOLATION AND IDENTIFICATION BY SEQUENCE HOMOLOGY OF A PUTATIVE CYTOSINE METHYLTRANSFERASE FROM ARABIDOPSIS THALIANA", NUCLEIC ACID RESEARCH, vol. 21, no. 10, 1993, pages 2383 - 2388, XP002049120 *
FINNEGAN, E.J., ET AL.: "REDUCED DNA METHYLATION IN ARABIDOPSIS THALIANA RESULTS IN ABNORMAL PLANT DEVELOPMENT", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 93, August 1996 (1996-08-01), pages 8449 - 8454, XP002049122 *
FINNEGAN, E.J.: "THE ROLE OF DNA METHYLATION IN PLANT DEVELOPMENT", EPIGENETIC MECHANISMS OF GENE REGULATION, vol. 32, 1996, COLD SPRING HARBOUR LABORATORY PRESS, pages 127 - 140, XP002049579 *
KAKUTANI, T., ET AL .: "CHARACTERIZATION OF AN ARABIDOPSIS THALIANA DNA HYPOMETHYLATION MUTANT", NUCLEIC ACID RESEARCH, vol. 23, no. 1, 1995, pages 130 - 137, XP002049118 *
RICHARDS, E.J., ET AL.: "DNA METHYLATION AND PLANT DEVELOPMENT", TRENDS IN GENETICS, vol. 13, no. 8, August 1997 (1997-08-01), pages 319 - 323, XP002049123 *
RONEMUS, M.J., ET AL.: "DEMETHYLATION-INDUCED DEVELOPMENTAL PLEIOTROPY IN ARABIDOPSIS", SCIENCE, vol. 273, 2 August 1996 (1996-08-02), pages 654 - 657, XP002049121 *
VONGS, A., ET AL.: "ARABIDOPSIS THALIANA DNA METHYLATION MUTANTS", SCIENCE, vol. 260, 25 July 1993 (1993-07-25), pages 1926 - 1928, XP002049119 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125857B2 (en) 1997-08-29 2006-10-24 The Regents Of The University Of California Modulators of DNA cytosine-5 methyltransferase and methods for use thereof
US7138384B1 (en) 1997-08-29 2006-11-21 The Regents Of The University Of California Modulators of DNA cytosine-5 methyltransferase and methods for use thereof
WO1999055891A1 (fr) * 1998-04-30 1999-11-04 Washington University Gene vegetal regulant la methylation de l'adn
EP1082417A4 (fr) * 1998-06-04 2006-08-23 Univ Queensland Sequences genetiques modifiant le phenotype
WO2001000801A3 (fr) * 1999-06-23 2001-05-10 Syngenta Participations Ag Gene intervenant dans l'inhibition epigenetique d'un gene
WO2001009299A3 (fr) * 1999-07-30 2001-08-23 Univ Bath Plantes modifiees
EP1690943A1 (fr) * 1999-07-30 2006-08-16 University Of Bath Plantes mofidiées
US7759546B2 (en) 1999-07-30 2010-07-20 University Of Bath Methods for modifying plant endosperm
WO2005038040A3 (fr) * 2003-10-14 2006-11-09 Ceres Inc Procedes et compositions permettant de modifier les phenotypes des semences
US7429692B2 (en) 2004-10-14 2008-09-30 Ceres, Inc. Sucrose synthase 3 promoter from rice and uses thereof

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