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WO1998005760A3 - Proteines pouvant etre induites en cas de privation de phosphate - Google Patents

Proteines pouvant etre induites en cas de privation de phosphate Download PDF

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Publication number
WO1998005760A3
WO1998005760A3 PCT/CA1997/000532 CA9700532W WO9805760A3 WO 1998005760 A3 WO1998005760 A3 WO 1998005760A3 CA 9700532 W CA9700532 W CA 9700532W WO 9805760 A3 WO9805760 A3 WO 9805760A3
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WO
WIPO (PCT)
Prior art keywords
phosphate
proteins
phosphate starvation
nucleic acids
inducible proteins
Prior art date
Application number
PCT/CA1997/000532
Other languages
English (en)
Other versions
WO1998005760A2 (fr
Inventor
Daniel D Lefebvre
Mohammad A Malboobi
Original Assignee
Univ Kingston
Daniel D Lefebvre
Mohammad A Malboobi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002182421A external-priority patent/CA2182421A1/fr
Priority claimed from US08/688,988 external-priority patent/US6096545A/en
Application filed by Univ Kingston, Daniel D Lefebvre, Mohammad A Malboobi filed Critical Univ Kingston
Priority to EP97932682A priority Critical patent/EP0917564A2/fr
Priority to BR9710909A priority patent/BR9710909A/pt
Priority to AU36167/97A priority patent/AU730471B2/en
Publication of WO1998005760A2 publication Critical patent/WO1998005760A2/fr
Publication of WO1998005760A3 publication Critical patent/WO1998005760A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Cette invention concerne des protéines, notamment des kinases, glucosidases et des agents de transport de phosphate, lesquelles sont exprimées dans des conditions de privation de phosphate. On décrit en outre des acides nucléiques et des constructions d'acides nucléiques codant ces protéines, des cellules contenant les acides nucléiques décrits et des organismes transgéniques de photosynthèse possédant une activité modifiée d'enzyme induite par phosphate.
PCT/CA1997/000532 1996-07-31 1997-07-30 Proteines pouvant etre induites en cas de privation de phosphate WO1998005760A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97932682A EP0917564A2 (fr) 1996-07-31 1997-07-30 Proteines pouvant etre induites en cas de privation de phosphate
BR9710909A BR9710909A (pt) 1996-07-31 1997-07-30 Acido desoxirribonucl-ico polipeptideo isolado  cido nucl-ico isolado vetor de expressÆo recombinante celula planta semente cultura de tecido organismo fotossint-tico c-lula procariÄtica descendente m-todo para produzir uma planta anticorpo m-todo para detectar a expressÆo de uma quinase de proteina m-todo para detectar a expressÆo de uma b-glucosidade sequencia de  cidos nucl-icos e m-todo para alterar a tolerancia ao frio ou ao congelamento em uma planta
AU36167/97A AU730471B2 (en) 1996-07-31 1997-07-30 Phosphate starvation-inducible proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/688,988 1996-07-31
CA002182421A CA2182421A1 (fr) 1996-07-31 1996-07-31 Proteines exprimees dans des conditions de carence en phosphate
CA2,182,421 1996-07-31
US08/688,988 US6096545A (en) 1996-07-31 1996-07-31 Phosphate starvation-inducible proteins

Publications (2)

Publication Number Publication Date
WO1998005760A2 WO1998005760A2 (fr) 1998-02-12
WO1998005760A3 true WO1998005760A3 (fr) 1998-10-08

Family

ID=25678591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1997/000532 WO1998005760A2 (fr) 1996-07-31 1997-07-30 Proteines pouvant etre induites en cas de privation de phosphate

Country Status (5)

Country Link
EP (1) EP0917564A2 (fr)
CN (1) CN1226925A (fr)
AU (1) AU730471B2 (fr)
BR (1) BR9710909A (fr)
WO (1) WO1998005760A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922564A (en) * 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
US6433249B1 (en) * 1998-11-10 2002-08-13 Pioneer Hi-Bred International, Inc. Use of β-glucosidase to enhance disease resistance and resistance to insects in crop plants
KR100401007B1 (ko) * 2000-08-11 2003-10-08 윤성중 담배의 인산수송자 유전자
EP1481058A2 (fr) * 2002-03-06 2004-12-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Polynucleotides codant une beta-glucosidase et utilisations associees
AU2005263730B2 (en) * 2004-07-16 2011-04-28 Cropdesign N.V. Plants having improved growth characteristics and method for making the same
EP2199394A1 (fr) * 2006-08-02 2010-06-23 CropDesign N.V. Plantes transformées avec un polypeptide-syt qui sous un stress abiotique, presentent un rendement amelioré, et leur procédé de production
US9055752B2 (en) 2008-11-06 2015-06-16 Intercontinental Great Brands Llc Shelf-stable concentrated dairy liquids and methods of forming thereof
UA112972C2 (uk) 2010-09-08 2016-11-25 Інтерконтінентал Грейт Брендс ЛЛС Рідкий молочний концентрат з високим вмістом сухих речовин
WO2014120663A1 (fr) 2013-01-29 2014-08-07 Creative Scientist, Llc Procédé de détermination de la sensibilité d'un individu à une faible dose de rayonnement ionisant
CN105219852B (zh) * 2015-09-25 2019-07-02 南京大学 一种检测鱼腥藻磷代谢相关酶基因表达量引物及应用与方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413920A (en) * 1992-04-08 1995-05-09 Purdue Research Foundation Method for enhanced production and recovery of phosphate starvation inducible gene products
WO1997035984A1 (fr) * 1996-03-25 1997-10-02 Mitsui Plant Biotechnology Research Institute Gene transporteur de phosphate vegetal et procede de regulation de la croissance d'une plante au moyen de ce gene
WO1998004701A1 (fr) * 1996-07-29 1998-02-05 Purdue Research Foundation Procedes et compositions pour ameliorer la capacite d'une plante a absorber le phosphate du sol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413920A (en) * 1992-04-08 1995-05-09 Purdue Research Foundation Method for enhanced production and recovery of phosphate starvation inducible gene products
WO1997035984A1 (fr) * 1996-03-25 1997-10-02 Mitsui Plant Biotechnology Research Institute Gene transporteur de phosphate vegetal et procede de regulation de la croissance d'une plante au moyen de ce gene
WO1998004701A1 (fr) * 1996-07-29 1998-02-05 Purdue Research Foundation Procedes et compositions pour ameliorer la capacite d'une plante a absorber le phosphate du sol

Non-Patent Citations (18)

* Cited by examiner, † Cited by third party
Title
B.L. EPEL ET AL.,: "A 41kDa protein isolated from maize mesocotyl cell walls immunolocalizes to plamodesmata", PROTOPLASMA, vol. 191, 1996, pages 70 - 78, XP002071949 *
C. FIFE AND D. LEFEBVRE: "Protein profiles of Brassica-nigra suspension cultures in response to phosphate stress", PLANT PHYSIOLOGY, vol. 89, no. 4 suppl., 1989, BETHESDA, MD, US, pages 178, XP002060425 *
C.A. FIFE ET AL.,: "The effect of phosphate deprivation on protein synthesis and fixed carbon storage reserves in Brassica nigra suspension cells", CANADIAN JOURNAL OF BOTANICS, vol. 68, 1990, OTTAWA, ONTARIO, CA, pages 1840 - 1847, XP002060427 *
DATABASE EMBL NUCLEOTIDE SEQUENCE, 1 November 1996 (1996-11-01), HINXTON, GB, XP002070419 *
DATABASE EMBL NUCLEOTIDE SEQUENCE, HINXTON, GB, XP002071153 *
DATABASE WPI Week 9745, 2 January 1997 Derwent World Patents Index; AN 97-489647, XP002070415 *
G. LEGGEWIE ET AL.,: "Two cDNA's from potato are able to complement a phosphate uptake deficient yeast mutant: Identification of phosphate transporters from higher plants", PLANT CELL., vol. 9, 1997, MD US, pages 381 - 392, XP002070420 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family", PLANT MOLECULAR BIOLOGY, vol. 34, no. 1, 1997, DORDRECHT, NL, pages 57 - 68, XP002061130 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "Differential gene expression at the transcriptional level in response to phosphate starvation in Brassica-nigra suspension cells", PLANT PHYSIOLOGY, vol. 102, no. 1 suppl., - 1993, ROCKVILLE, US, pages 9, XP002060426 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells", PLANT MOLECULAR BIOLOGY, vol. 28, no. 5, 1995, BE, pages 859 - 870, XP002061861 *
M.A. MALBOOBI ET AL.,: "Identification and nucleotide sequences of cDNA clones of phosphate inducible beta-glucosidase genes of Brassicaceae (Accession Nos. U72153 and U72154) (PGR96-114)", PLANT PHYSIOLOGY, vol. 112, 26 August 1996 (1996-08-26), pages 1399 - 1399, XP002061129 *
M.J. HAWKESFORD AND A.R. BELCHER: "Differential protein synthesis in response to sulphate and phosphate deprivation: Identification of possible components of plasma-membrane transport systems in cultured tomato roots", PLANTA, vol. 185, no. 3, 1991, BERLIN, DE, pages 323 - 329, XP002070416 *
N. MITSUKAWA ET AL.,: "Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 94, 1997, WASHINGTON, DC, US, pages 7098 - 7102, XP002070505 *
P. BUDICKY ET AL.,: "Identification of a putative phosphate starvation inducible protein kinase from Brassica nigra", PLANT PHYSIOLOGY, vol. 102, no. 1 suppl., - 1993, ROCKVILLE, US, pages 68, XP002060422 *
T. NEWMAN ET AL.,: "Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones", PLANT PHYSIOLOGY, vol. 106, 1994, pages 1241 - 1255, XP002004690 *
U.S. MUCHHAL ET AL.,: "Phosphate transporters from the higher plant Arabidopsis thaliana", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 93, no. 19, 1996, WASHINGTON, DC, US, pages 10519 - 10523, XP002070417 *
Y.S. PARK ET AL.,: "Frequent in-frame lenght variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus", PLANT MOLECULAR BIOLOGY, vol. 27, 1995, DORDRECHT, NL, pages 829 - 833, XP002060428 *
Y.S. PARK ET AL.,: "Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains", PLANT MOLECULAR BIOLOGY, vol. 22, 1993, DORDRECHT, NL, pages 615 - 624, XP002060429 *

Also Published As

Publication number Publication date
AU3616797A (en) 1998-02-25
BR9710909A (pt) 1999-08-17
EP0917564A2 (fr) 1999-05-26
AU730471B2 (en) 2001-03-08
CN1226925A (zh) 1999-08-25
WO1998005760A2 (fr) 1998-02-12

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