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WO1998011259A3 - Composition, method and kit for detection and identification of sexually transmitted disease microorganisms - Google Patents

Composition, method and kit for detection and identification of sexually transmitted disease microorganisms Download PDF

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Publication number
WO1998011259A3
WO1998011259A3 PCT/CA1997/000660 CA9700660W WO9811259A3 WO 1998011259 A3 WO1998011259 A3 WO 1998011259A3 CA 9700660 W CA9700660 W CA 9700660W WO 9811259 A3 WO9811259 A3 WO 9811259A3
Authority
WO
WIPO (PCT)
Prior art keywords
sexually transmitted
transmitted disease
gene fragments
amplification
detection
Prior art date
Application number
PCT/CA1997/000660
Other languages
French (fr)
Other versions
WO1998011259A2 (en
Inventor
Max Chernesky
Kathleen Luinstra
Dan Jang
Sylvia Chong
James B Mahony
James M Dunn
Original Assignee
Visible Genetics Inc
Max Chernesky
Kathleen Luinstra
Dan Jang
Sylvia Chong
James B Mahony
James M Dunn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Visible Genetics Inc, Max Chernesky, Kathleen Luinstra, Dan Jang, Sylvia Chong, James B Mahony, James M Dunn filed Critical Visible Genetics Inc
Priority to AU41962/97A priority Critical patent/AU4196297A/en
Publication of WO1998011259A2 publication Critical patent/WO1998011259A2/en
Publication of WO1998011259A3 publication Critical patent/WO1998011259A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Co-amplification and detection of at least three sexually transmitted disease pathogens in DNA isolated from a patient sample are achieved by combining the isolated DNA with at least first, second and third oligonucleotide primer pairs for amplification of a gene fragments from first, second and third different sexually transmitted disease pathogens. One of the primers in each primer pair is labeled with a detectable label. The first, second and third oligonucleotide primer pairs are reacted with the isolated DNA in an amplification reaction to generate an amplification product mixture that contains amplified gene fragments from either or both of the first and second sexually transmitted disease pathogens, depending on whether or not the first and second sexually transmitted disease pathogens are present in the patient sample. Amplified gene fragments in the amplification product mixture are then evaluated, for example by gel electrophoresis to determine whether amplified gene fragments from the first, second or third sexually transmitted disease pathogen are present.
PCT/CA1997/000660 1996-09-09 1997-09-09 Composition, method and kit for detection and identification of sexually transmitted disease microorganisms WO1998011259A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU41962/97A AU4196297A (en) 1996-09-09 1997-09-09 Composition, method and kit for detection and identification of sexually transmitted disease microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70883296A 1996-09-09 1996-09-09
US08/708,832 1996-09-09

Publications (2)

Publication Number Publication Date
WO1998011259A2 WO1998011259A2 (en) 1998-03-19
WO1998011259A3 true WO1998011259A3 (en) 1998-04-23

Family

ID=24847349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1997/000660 WO1998011259A2 (en) 1996-09-09 1997-09-09 Composition, method and kit for detection and identification of sexually transmitted disease microorganisms

Country Status (2)

Country Link
AU (1) AU4196297A (en)
WO (1) WO1998011259A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778611B2 (en) 2006-05-26 2014-07-15 The Secretary Of State For Health Mycoplasma genitalium detection assay based on the Mg219 gene

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1042323B (en) * 1998-09-24 2009-08-07 Fujirebio Europe N.V. Identification of microorganisms causing acute respiratory tract infections (ari)
KR100619189B1 (en) * 2004-10-08 2006-08-31 굿젠 주식회사 DNA chip, assay kit, and genotyping method for genotyping of progenitors for sexually transmitted disease
US9139883B2 (en) 2005-08-17 2015-09-22 University Of Delhi PCR-based detection method for Chlamydia trachomatis
US20090035775A1 (en) * 2005-08-17 2009-02-05 University Of Delhi Pcr-based detection method for chlamydia treachomatis
GB2431469A (en) * 2005-10-19 2007-04-25 Ivan Petyaev Pathogen diagnosis
KR100860619B1 (en) * 2006-02-23 2008-09-29 주식회사 씨젠 Oligonucleotides for Detection of Adult Disease-Induced Pathogen Nucleic Acids
EP1862557B1 (en) * 2006-05-29 2011-05-18 Bio-Rad Pasteur Real-time multiplex detection of three bacterial species responsible for sexually-transmitted diseases
KR101101880B1 (en) * 2008-11-25 2012-01-05 문우철 DNA chips, kits for detecting, genotyping and antibiotic-resistant genotypes, and methods of detection and genotyping using the same
GB201004235D0 (en) 2010-03-15 2010-04-28 Knight Scient Ltd Method for detection of inflammation in the urinary tract or urethra
KR20110105663A (en) * 2010-03-19 2011-09-27 주식회사 진진바이오 Method and kit for detecting female genital pathogens using multiplex liquid array system
CN112458195B (en) * 2020-12-28 2023-03-24 广州迈景基因医学科技有限公司 Multiplex PCR primer set, kit and method for detecting sexually transmitted pathogens based on high-throughput sequencing
CN113186316A (en) * 2021-05-26 2021-07-30 北京华诺奥美基因生物科技有限公司 Mycoplasma genitalium nucleic acid real-time fluorescence PCR detection primer, probe and kit
CN113215322B (en) * 2021-05-26 2022-09-30 北京毅新博创生物科技有限公司 Primer composition for detecting genital tract pathogen by MALDI-TOF MS and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648845A2 (en) * 1993-07-08 1995-04-19 Johnson & Johnson Clinical Diagnostics, Inc. Method for coamplification of two different nucleic acid sequences using polymerase chain reaction
EP0687737A1 (en) * 1994-05-16 1995-12-20 F. Hoffmann-La Roche AG Detection of herpes simplex virus, treponema pallidum and haemophilus ducreyi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648845A2 (en) * 1993-07-08 1995-04-19 Johnson & Johnson Clinical Diagnostics, Inc. Method for coamplification of two different nucleic acid sequences using polymerase chain reaction
EP0687737A1 (en) * 1994-05-16 1995-12-20 F. Hoffmann-La Roche AG Detection of herpes simplex virus, treponema pallidum and haemophilus ducreyi

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 125, no. 19, 4 November 1996, Columbus, Ohio, US; abstract no. 239517k, ZENG, XINGWU ET AL: "Rapid detection of mixed infection of Neisseria gonorrhoeae, Chlymidia trachomatis, Ureaplasma urealyticum by multiplex-PCR" page 271; column left; XP002056582 *
DE BARBEYRAC B ET AL: "Microbiological study of pelvic inflammatory disease (PID): Report on a french cohort", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 96, no. 0, 19 May 1996 (1996-05-19) - 23 May 1996 (1996-05-23), pages 286, XP002056566 *
MAHONY J ET AL: "Detection of C. trachomatis (CT), N. gonorrhoeae (NG), U. urealyticum (UU), M. genitalium (MG), in urine specimens by multiplex PCR (M-PCR)", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 96, no. 0, 19 May 1996 (1996-05-19) - 23 May 1996 (1996-05-23), pages 9, XP002056565 *
MITRANI-ROSENBAUM S ET AL: "SIMULTANEOUS DETECTION OF THREE COMMON SEXUALLY TRANSMITTED AGENTS BY POLYMERASE CHAIN REACTION", AMERICAN JOURNAL OF OBSTETRICS & GYNECOLOGY, vol. 171, no. 3, September 1994 (1994-09-01), pages 784 - 790, XP000612760 *
STEWART R ET AL: "Use of digoxigenin dUTP to increase detection sensitivity of PCR assays for common sexual diseases", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 94, no. 0, 23 May 1994 (1994-05-23) - 27 May 1995 (1995-05-27), pages 568, XP002056567 *
ZENG, XINGWU ET AL, ZHONGHUA YIXUE JIANYAN ZAZHI, vol. 19, no. 3, February 1996 (1996-02-01), pages 176 - 78 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778611B2 (en) 2006-05-26 2014-07-15 The Secretary Of State For Health Mycoplasma genitalium detection assay based on the Mg219 gene

Also Published As

Publication number Publication date
WO1998011259A2 (en) 1998-03-19
AU4196297A (en) 1998-04-02

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