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WO1998011238A3 - Protease m, a novel serine protease - Google Patents

Protease m, a novel serine protease Download PDF

Info

Publication number
WO1998011238A3
WO1998011238A3 PCT/US1997/016175 US9716175W WO9811238A3 WO 1998011238 A3 WO1998011238 A3 WO 1998011238A3 US 9716175 W US9716175 W US 9716175W WO 9811238 A3 WO9811238 A3 WO 9811238A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
nucleic acid
acid molecules
expression vectors
tumor cells
Prior art date
Application number
PCT/US1997/016175
Other languages
French (fr)
Other versions
WO1998011238A2 (en
WO1998011238A9 (en
Inventor
Anthony Anisowicz
Ruth Sager
Georgia Sotiropoulou
Original Assignee
Dana Farber Cancer Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dana Farber Cancer Inst Inc filed Critical Dana Farber Cancer Inst Inc
Priority to AU44807/97A priority Critical patent/AU4480797A/en
Publication of WO1998011238A2 publication Critical patent/WO1998011238A2/en
Publication of WO1998011238A3 publication Critical patent/WO1998011238A3/en
Publication of WO1998011238A9 publication Critical patent/WO1998011238A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Isolated nucleic acid molecules encoding a novel serine protease, Protease M, is disclosed. Protease M is downregulated in metastatic mammary epithelial tumor cells, as well as other tumor cells, and is upregulated in senescent cells. In addition to isolated nucleic acid molecules, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a Protease M gene has been introduced or disrupted. The invention further provides isolated Protease M proteins, fusion proteins, antigenic peptides and anti-Protease M antibodies. Diagnostic assays, drug screening assays, and therapeutic methods utilizing compositions of the invention are also provided.
PCT/US1997/016175 1996-09-13 1997-09-11 Protease m, a novel serine protease WO1998011238A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU44807/97A AU4480797A (en) 1996-09-13 1997-09-11 Protease m, a novel serine protease

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2530196P 1996-09-13 1996-09-13
US60/025,301 1996-09-13

Publications (3)

Publication Number Publication Date
WO1998011238A2 WO1998011238A2 (en) 1998-03-19
WO1998011238A3 true WO1998011238A3 (en) 1998-06-04
WO1998011238A9 WO1998011238A9 (en) 1998-07-09

Family

ID=21825221

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/016175 WO1998011238A2 (en) 1996-09-13 1997-09-11 Protease m, a novel serine protease

Country Status (2)

Country Link
AU (1) AU4480797A (en)
WO (1) WO1998011238A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000001807A1 (en) * 1998-07-02 2000-01-13 Fuso Pharmaceutical Industries, Ltd. Serine protease-specific monoclonal antibody and utilization thereof
US6322962B1 (en) 1998-08-14 2001-11-27 Board Of Regents, The University Of Texas System Sterol-regulated Site-1 protease and assays of modulators thereof
JP2001054399A (en) 1999-08-18 2001-02-27 Fuji Photo Film Co Ltd Judgement of effectiveness of drug having protease inhibiting action
CN1298014A (en) * 1999-11-29 2001-06-06 上海博容基因开发有限公司 Human ATP-dependent serine proteolytic enzyme 48 as one new kind of polypeptide and polynucleotides encoding this polypeptide
CN1322833A (en) * 2000-05-09 2001-11-21 上海博德基因开发有限公司 New polypeptide human ATP dependent serine proteinase 18 and its encoding polynucleotides
AU2001271878A1 (en) * 2000-07-06 2002-01-21 Deltagen, Inc. Transgenic mice containing targeted gene disruptions
CA2360219A1 (en) 2000-10-27 2002-04-27 Mount Sinai Hospital Method for detecting alzheimer's disease
ES2287169T3 (en) * 2000-10-27 2007-12-16 Mount Sinai Hospital PROCEDURE FOR DETECTING OVARY CANCER BASED ON HUMAN CALICREIN 6 (HK6).
WO2003034068A1 (en) * 2001-10-16 2003-04-24 Mount Sinai Hospital Localization of disorders associated with kallikreins
KR20040081420A (en) * 2001-10-16 2004-09-21 마운트 시나이 하스피틀 코포레이션 Methods for detecting ovarian cancer
WO2003037258A2 (en) * 2001-10-31 2003-05-08 Millennium Pharmaceuticals, Inc. Methods and compositions for the treatment and diagnosis of pain disorders using 2047
ES2316770T3 (en) * 2002-05-21 2009-04-16 Mayo Foundation For Medical Education And Research PROCEDURE AND MATERIALS FOR THE TREATMENT AND DIAGNOSIS OF INFLAMMATORY AFFECTIONS RELATED TO KALICREINA-6.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005188A1 (en) * 1988-11-11 1990-05-17 Pharmaceutical Proteins Limited A genetic construct of which protein-coding dna comprises introns and is designed for protein production in transgenic animals
EP0576152A1 (en) * 1992-05-28 1993-12-29 Eli Lilly And Company Protease and related DNA compounds
EP0652014A1 (en) * 1993-11-10 1995-05-10 National Institute Of Immunology Treatment of prostatic hypertrophy
WO1996026280A1 (en) * 1995-02-21 1996-08-29 Basf Aktiengesellschaft NOVEL CYSTEINE PROTEASE RELATED TO INTERLEUKIN-1β CONVERTING ENZYME

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005188A1 (en) * 1988-11-11 1990-05-17 Pharmaceutical Proteins Limited A genetic construct of which protein-coding dna comprises introns and is designed for protein production in transgenic animals
EP0576152A1 (en) * 1992-05-28 1993-12-29 Eli Lilly And Company Protease and related DNA compounds
EP0652014A1 (en) * 1993-11-10 1995-05-10 National Institute Of Immunology Treatment of prostatic hypertrophy
WO1996026280A1 (en) * 1995-02-21 1996-08-29 Basf Aktiengesellschaft NOVEL CYSTEINE PROTEASE RELATED TO INTERLEUKIN-1β CONVERTING ENZYME

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANISOWICZ A ET AL: "A novel protease homolog differentially expressed in breast and ovarian cancer", MOLECULAR MEDICINE (CAMBRIDGE), 2 (5).30 SEP 1996. 624-636., XP002060076 *
HAGEN HE ET AL: "Simulium damnosum s.l.: identification of inducible serine proteases following an Onchocerca infection by differential display reverse transcription PCR.", EXP PARASITOL, NOV 1995, 81 (3) P249-54, UNITED STATES, XP002060075 *
VIHINEN M: "Modeling of prostate specific antigen and human glandular kallikrein structures.", BIOCHEM BIOPHYS RES COMMUN, NOV 15 1994, 204 (3) P1251-6, UNITED STATES, XP002060074 *

Also Published As

Publication number Publication date
WO1998011238A2 (en) 1998-03-19
AU4480797A (en) 1998-04-02

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