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WO1998037180A3 - Hcv fusion protease and polynucleotide encoding same - Google Patents

Hcv fusion protease and polynucleotide encoding same Download PDF

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Publication number
WO1998037180A3
WO1998037180A3 PCT/US1998/003367 US9803367W WO9837180A3 WO 1998037180 A3 WO1998037180 A3 WO 1998037180A3 US 9803367 W US9803367 W US 9803367W WO 9837180 A3 WO9837180 A3 WO 9837180A3
Authority
WO
WIPO (PCT)
Prior art keywords
encoding same
polynucleotide encoding
protease
hcv fusion
fusion protease
Prior art date
Application number
PCT/US1998/003367
Other languages
French (fr)
Other versions
WO1998037180A2 (en
Inventor
Chih-Ming Chen
Akhteruzzaman Molla
Rakesh L Tripathi
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Publication of WO1998037180A2 publication Critical patent/WO1998037180A2/en
Publication of WO1998037180A3 publication Critical patent/WO1998037180A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides biologically active fusion proteins of hepatitis C virus NS3 protease and NS4A cofactor protein which are non-autocleavable and polynucleotides encoding same. Expression vectors comprising those polynucleotides and host cells transformed with those polynucleotides are also disclosed. The invention also provides a method for identifying inhibitor compounds of hepatitis C virus NS3 protease using the disclosed fusion proteins.
PCT/US1998/003367 1997-02-22 1998-02-20 Hcv fusion protease and polynucleotide encoding same WO1998037180A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80426697A 1997-02-22 1997-02-22
US08/804,266 1997-02-22

Publications (2)

Publication Number Publication Date
WO1998037180A2 WO1998037180A2 (en) 1998-08-27
WO1998037180A3 true WO1998037180A3 (en) 1998-11-19

Family

ID=25188568

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/003367 WO1998037180A2 (en) 1997-02-22 1998-02-20 Hcv fusion protease and polynucleotide encoding same

Country Status (1)

Country Link
WO (1) WO1998037180A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223743B2 (en) 2000-08-17 2007-05-29 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
US7226912B2 (en) 2000-08-17 2007-06-05 Tripep Ab Hepatitis C virus non-structural NS3/4A fusion gene
US7244422B2 (en) 2000-08-29 2007-07-17 Tripep Ab Vaccines containing ribavirin and methods of use thereof
US8883169B2 (en) 2007-08-16 2014-11-11 Chrontech Pharma Ab Immunogen platform

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280940B1 (en) 1998-08-05 2001-08-28 Agouron Pharmaceuticals, Inc. Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease
WO2001002601A2 (en) * 1999-07-07 2001-01-11 Du Pont Pharmaceuticals Company Cell-based assay systems for examining hcv ns3 protease activity
US7244721B2 (en) 2000-07-21 2007-07-17 Schering Corporation Peptides as NS3-serine protease inhibitors of hepatitis C virus
KR20030081297A (en) 2000-07-21 2003-10-17 쉐링 코포레이션 Novel peptides as NS3-serine protease inhibitors of hepatitis C virus
MXPA03000627A (en) 2000-07-21 2004-07-30 Schering Corp Novel peptides as ns3-serine protease inhibitors of hepatitis c virus.
WO2002013855A2 (en) * 2000-08-17 2002-02-21 Tripep Ab Vaccines containing ribavirin and methods of use thereof
CN108239656A (en) * 2016-12-27 2018-07-03 天津天锐生物科技有限公司 A kind of protein function switching system of small-molecule drug control
JP2022518488A (en) * 2019-01-25 2022-03-15 センティ バイオサイエンシズ インコーポレイテッド Fusion constructs for controlling protein function

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022985A1 (en) * 1994-02-23 1995-08-31 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the proteolytic activity of the ns3 protease of hepatitis c virus (hcv)
WO1996036702A2 (en) * 1995-05-12 1996-11-21 Schering Corporation Soluble, active hepatitis c virus protease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022985A1 (en) * 1994-02-23 1995-08-31 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for reproducing in vitro the proteolytic activity of the ns3 protease of hepatitis c virus (hcv)
WO1996036702A2 (en) * 1995-05-12 1996-11-21 Schering Corporation Soluble, active hepatitis c virus protease

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"GST Gene Fusion Vectors", PHARMCIA BIOTECH '95 '96 CATALOGUE, 1994, pages 118 - 119, XP002077832 *
KOLYKHALOV, A.A. ET AL.: "Specificity of the Hepatitis C Virus NS3 serine protease: Effects of substitutions at the 3/4A, 4A/4B, 4B/5A, and 5A/5B cleavage sites on polyprotein processing", JOURNAL OF VIROLOGY, vol. 68, no. 11, November 1994 (1994-11-01), pages 7525 - 7533, XP002077834 *
STEINKÜHLER, C. ET AL.: "Activity of purified Hepatitis C Virus protease NS3 on peptide substrates", JOURNAL OF VIROLOGY, vol. 70, no. 10, October 1996 (1996-10-01), pages 6694 - 6700, XP002077833 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223743B2 (en) 2000-08-17 2007-05-29 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
US7226912B2 (en) 2000-08-17 2007-06-05 Tripep Ab Hepatitis C virus non-structural NS3/4A fusion gene
US7241440B2 (en) 2000-08-17 2007-07-10 Tripep Ab Vaccines containing ribavirin and methods of use thereof
US7307066B2 (en) 2000-08-17 2007-12-11 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
US7439347B2 (en) 2000-08-17 2008-10-21 Tripep Ab Hepatitis C virus non-structural NS3/4A fusion gene
US7638499B2 (en) 2000-08-17 2009-12-29 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
US7244422B2 (en) 2000-08-29 2007-07-17 Tripep Ab Vaccines containing ribavirin and methods of use thereof
US7261883B2 (en) 2000-08-29 2007-08-28 Tripep Ab Vaccines containing ribavirin and methods of use thereof
US8883169B2 (en) 2007-08-16 2014-11-11 Chrontech Pharma Ab Immunogen platform

Also Published As

Publication number Publication date
WO1998037180A2 (en) 1998-08-27

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