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WO1998039425A3 - Vectors and methods for expression of mutant proteins - Google Patents

Vectors and methods for expression of mutant proteins Download PDF

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Publication number
WO1998039425A3
WO1998039425A3 PCT/US1998/004155 US9804155W WO9839425A3 WO 1998039425 A3 WO1998039425 A3 WO 1998039425A3 US 9804155 W US9804155 W US 9804155W WO 9839425 A3 WO9839425 A3 WO 9839425A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutant
toxin
mutants
expression
fusion proteins
Prior art date
Application number
PCT/US1998/004155
Other languages
French (fr)
Other versions
WO1998039425A2 (en
Inventor
David M Neville
Original Assignee
Us Health
David M Neville
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, David M Neville filed Critical Us Health
Priority to CA002283497A priority Critical patent/CA2283497A1/en
Priority to EP98910152A priority patent/EP0968282A2/en
Priority to AU64459/98A priority patent/AU736501B2/en
Publication of WO1998039425A2 publication Critical patent/WO1998039425A2/en
Publication of WO1998039425A3 publication Critical patent/WO1998039425A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention describes a new shuttle vector permitting the expression of engineered toxin mutants and toxin fusion proteins in Corynebacterium. In addition the invention provides a mutant Pichia pastoris, a method for producing this mutant and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant form of Pichia pastoris. The invention further provides a mutant Chinese hamster ovary (CHO) cell, a mutant insect cell, a method for producing these mutants and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant cells.
PCT/US1998/004155 1997-03-05 1998-03-05 Vectors and methods for expression of mutant proteins WO1998039425A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002283497A CA2283497A1 (en) 1997-03-05 1998-03-05 Vectors and methods for expression of mutant proteins
EP98910152A EP0968282A2 (en) 1997-03-05 1998-03-05 Vectors and methods for expression of mutant proteins
AU64459/98A AU736501B2 (en) 1997-03-05 1998-03-05 Novel vectors and expression methods for producing mutant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3719697P 1997-03-05 1997-03-05
US60/037,196 1997-03-05

Publications (2)

Publication Number Publication Date
WO1998039425A2 WO1998039425A2 (en) 1998-09-11
WO1998039425A3 true WO1998039425A3 (en) 1999-01-14

Family

ID=21892966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/004155 WO1998039425A2 (en) 1997-03-05 1998-03-05 Vectors and methods for expression of mutant proteins

Country Status (4)

Country Link
EP (1) EP0968282A2 (en)
AU (1) AU736501B2 (en)
CA (1) CA2283497A1 (en)
WO (1) WO1998039425A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7696338B2 (en) 1995-10-30 2010-04-13 The United States Of America As Represented By The Department Of Health And Human Services Immunotoxin fusion proteins and means for expression thereof
US7517527B2 (en) 1995-10-30 2009-04-14 The United States Of America As Represented By The Department Of Health And Human Services Immunotoxin with in vivo T cell suppressant activity and methods of use
WO2001087982A2 (en) * 2000-05-18 2001-11-22 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Immunotoxin fusion proteins and means for expression thereof
US7288254B2 (en) 1995-10-30 2007-10-30 The United States Of America As Represented By The Secretary, Department Of Health And Human Services, Nih Use of immunotoxins to induce immune tolerance to pancreatic islet transplantation
US7125553B1 (en) 1996-04-15 2006-10-24 The United States of America as represented by the Department of Health and Human Services c/o Centers for Disease Control and Prevention Methods of inducing immune tolerance using immunotoxins
EP1015496B1 (en) 1997-03-05 2009-06-17 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES Divalent anti-t cells immunotoxins and use thereof
US20180303877A1 (en) * 2014-09-25 2018-10-25 The General Hospital Corporation Cell-based targeted delivery of pseudonomas exotoxin

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0217327A2 (en) * 1985-09-30 1987-04-08 Sumitomo Pharmaceuticals Company, Limited DNA Sequence encoding variant elongation factor 2
WO1987002987A1 (en) * 1985-11-13 1987-05-21 Murphy John R Cys codon-modified dna
EP0332174A2 (en) * 1988-03-08 1989-09-13 The University Of Wyoming Cell specific cytotoxic agents
WO1991013157A1 (en) * 1990-02-26 1991-09-05 Commonwealth Scientific And Industrial Research Organisation Shuttle plasmid for escherichia coli and mycobacteria
DE4024187A1 (en) * 1990-07-30 1992-02-06 Biotechnolog Forschung Gmbh Mutagenesis of plasmids in form of gapped circles - by hybridisation with mutagenic oligo:nucleotide, then selective linearisation of non-mutated circles
WO1992013562A1 (en) * 1991-02-11 1992-08-20 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce An immunotoxin with in vivo t cell suppressant activity
WO1993015113A1 (en) * 1992-01-24 1993-08-05 Tanox Biosystems, Inc. An immunotoxin including a cytotoxin with an unpaired cysteine residue in or near its receptor-binding site
EP0616034A2 (en) * 1993-03-05 1994-09-21 American Cyanamid Company Novel plasmid for production of CRM protein and diphtheria toxin
WO1995033481A1 (en) * 1994-06-08 1995-12-14 President And Fellows Of Harvard College Diphtheria toxin vaccines bearing a mutated r domain
WO1996032137A2 (en) * 1995-04-14 1996-10-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Methods of inducing immune tolerance using immunotoxins

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0217327A2 (en) * 1985-09-30 1987-04-08 Sumitomo Pharmaceuticals Company, Limited DNA Sequence encoding variant elongation factor 2
WO1987002987A1 (en) * 1985-11-13 1987-05-21 Murphy John R Cys codon-modified dna
EP0332174A2 (en) * 1988-03-08 1989-09-13 The University Of Wyoming Cell specific cytotoxic agents
WO1991013157A1 (en) * 1990-02-26 1991-09-05 Commonwealth Scientific And Industrial Research Organisation Shuttle plasmid for escherichia coli and mycobacteria
DE4024187A1 (en) * 1990-07-30 1992-02-06 Biotechnolog Forschung Gmbh Mutagenesis of plasmids in form of gapped circles - by hybridisation with mutagenic oligo:nucleotide, then selective linearisation of non-mutated circles
WO1992013562A1 (en) * 1991-02-11 1992-08-20 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce An immunotoxin with in vivo t cell suppressant activity
WO1993015113A1 (en) * 1992-01-24 1993-08-05 Tanox Biosystems, Inc. An immunotoxin including a cytotoxin with an unpaired cysteine residue in or near its receptor-binding site
EP0616034A2 (en) * 1993-03-05 1994-09-21 American Cyanamid Company Novel plasmid for production of CRM protein and diphtheria toxin
WO1995033481A1 (en) * 1994-06-08 1995-12-14 President And Fellows Of Harvard College Diphtheria toxin vaccines bearing a mutated r domain
WO1996032137A2 (en) * 1995-04-14 1996-10-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Methods of inducing immune tolerance using immunotoxins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CREGG J M ET AL: "3. Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins", DEVELOPMENTS IN INDUSTRIAL MICROBIOLOGY (JOURNAL OF INDUSTRIAL MICROBIOLOGY, SUPPL. NO. 3), vol. 29, 1988, pages 33 - 41, XP002082180 *
KIMATA Y ET AL.: "Expression of non-ADP-ribosylatable, diphteria toxin-resistant elongation factor 2 in Saccharomyces cerevisiae", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS., vol. 191, no. 3, 31 March 1993 (1993-03-31), ORLANDO, FL US, pages 1145 - 1151, XP002082179 *
PERENTESIS J P ET AL: "PROTEIN TOXIN INHIBITORS OF PROTEIN SYNTHESIS", BIOFACTORS, vol. 3, no. 3, January 1992 (1992-01-01), OXFORD GB, pages 173 - 184, XP000611278 *

Also Published As

Publication number Publication date
CA2283497A1 (en) 1998-09-11
AU6445998A (en) 1998-09-22
EP0968282A2 (en) 2000-01-05
AU736501B2 (en) 2001-07-26
WO1998039425A2 (en) 1998-09-11

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