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WO1999053031A3 - Process for producing cell clone libraries - Google Patents

Process for producing cell clone libraries Download PDF

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Publication number
WO1999053031A3
WO1999053031A3 PCT/EP1999/002391 EP9902391W WO9953031A3 WO 1999053031 A3 WO1999053031 A3 WO 1999053031A3 EP 9902391 W EP9902391 W EP 9902391W WO 9953031 A3 WO9953031 A3 WO 9953031A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell clone
producing cell
clone libraries
cell
cell clones
Prior art date
Application number
PCT/EP1999/002391
Other languages
French (fr)
Other versions
WO1999053031A2 (en
Inventor
Teymuras Kurzchalia
Original Assignee
Max Planck Gesellschaft
Teymuras Kurzchalia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Teymuras Kurzchalia filed Critical Max Planck Gesellschaft
Priority to AU37070/99A priority Critical patent/AU3707099A/en
Publication of WO1999053031A2 publication Critical patent/WO1999053031A2/en
Publication of WO1999053031A3 publication Critical patent/WO1999053031A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Described is a process for producing cell clone libraries via the use of a pool of expression vectors comprising ribozyme genes. Furthermore, cell clones with modified phenotypes and non-human animals derived from such cell clones are provided as well as novel genes identified from the cell clone library. Fields of the application of the invention are molecular-biological research and the pharmaceutical industry.
PCT/EP1999/002391 1998-04-08 1999-04-08 Process for producing cell clone libraries WO1999053031A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU37070/99A AU3707099A (en) 1998-04-08 1999-04-08 Process for producing cell clone libraries

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19815590 1998-04-08
DE19815590.5 1998-04-08

Publications (2)

Publication Number Publication Date
WO1999053031A2 WO1999053031A2 (en) 1999-10-21
WO1999053031A3 true WO1999053031A3 (en) 1999-12-16

Family

ID=7863891

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/002391 WO1999053031A2 (en) 1998-04-08 1999-04-08 Process for producing cell clone libraries

Country Status (2)

Country Link
AU (1) AU3707099A (en)
WO (1) WO1999053031A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787646B1 (en) 1999-11-12 2004-09-07 Immusol, Inc. Tumor suppressor molecules and methods of use

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993011230A1 (en) * 1991-12-02 1993-06-10 Dynal As Modified mammalian stem cell blocking viral replication
WO1994013833A1 (en) * 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Ribozyme amplified diagnostics
DE4424762C1 (en) * 1994-07-04 1995-07-27 Max Planck Gesellschaft Ribozyme library in optimised expression cassette
WO1997027213A1 (en) * 1996-01-23 1997-07-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and rna molecules
WO1998032880A1 (en) * 1997-01-23 1998-07-30 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993011230A1 (en) * 1991-12-02 1993-06-10 Dynal As Modified mammalian stem cell blocking viral replication
WO1994013833A1 (en) * 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Ribozyme amplified diagnostics
DE4424762C1 (en) * 1994-07-04 1995-07-27 Max Planck Gesellschaft Ribozyme library in optimised expression cassette
WO1997027213A1 (en) * 1996-01-23 1997-07-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and rna molecules
WO1998032880A1 (en) * 1997-01-23 1998-07-30 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HASELOFF J ET AL: "SIMPLE RNA ENZYMES WITH NEW AND HIGHLY SPECIFIC ENDORIBONUCLEASE ACTIVITIES", NATURE, vol. 334, 18 August 1988 (1988-08-18), pages 585 - 591, XP002029776, ISSN: 0028-0836 *
JARVIS T C ET AL: "INHIBITION OF VASCULAR SMOOTH MUSCLE CELL PROLIFERATION BY HAMMERHEAD RIBOZYMES TARGETTING C-MYB", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 19A, 1 January 1995 (1995-01-01), pages 221, XP002024063, ISSN: 0730-2312 *
LARSSON S ET AL.,: "Reduced beta2-microglobulin mRNA levels in transgenic mice expressing a designed hammerhead ribozyme", NUCLEIC ACIDS RESEARCH, vol. 22, no. 12, 1994, pages 2242 - 2248, XP002120162 *
SKRYABIN BV AND SCHMAUSS C: "Enhanced selection fro homologous-recombinant embryonic stem cell clones with a neomycin phosphotransferase gene in antisense orientation", TRANSGENIC RESEARCH, vol. 6, 1997, pages 27 - 35, XP002120163 *
XIE Y F ET AL: "A ribozyme -mediated, gene ''knockdown'' strategy for the identification of gene function in zebrafish", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, no. 25, 9 December 1997 (1997-12-09), pages 13777 - 13781, XP002108912, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO1999053031A2 (en) 1999-10-21
AU3707099A (en) 1999-11-01

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