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WO1999063111A3 - Methode de marquage d'acide nucleique - Google Patents

Methode de marquage d'acide nucleique Download PDF

Info

Publication number
WO1999063111A3
WO1999063111A3 PCT/IB1999/001116 IB9901116W WO9963111A3 WO 1999063111 A3 WO1999063111 A3 WO 1999063111A3 IB 9901116 W IB9901116 W IB 9901116W WO 9963111 A3 WO9963111 A3 WO 9963111A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
oligonucleotide
conjugates
peptide
labelling nucleic
Prior art date
Application number
PCT/IB1999/001116
Other languages
English (en)
Other versions
WO1999063111A2 (fr
Inventor
Ramon Eritja
Dirk Gottschling
Hartmut Seliger
Original Assignee
European Molecular Biology Lab Embl
Ramon Eritja
Dirk Gottschling
Hartmut Seliger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9811771.6A external-priority patent/GB9811771D0/en
Priority claimed from GBGB9813765.6A external-priority patent/GB9813765D0/en
Application filed by European Molecular Biology Lab Embl, Ramon Eritja, Dirk Gottschling, Hartmut Seliger filed Critical European Molecular Biology Lab Embl
Priority to AU39525/99A priority Critical patent/AU3952599A/en
Publication of WO1999063111A2 publication Critical patent/WO1999063111A2/fr
Publication of WO1999063111A3 publication Critical patent/WO1999063111A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B32—LAYERED PRODUCTS
    • B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
    • B32B27/00—Layered products comprising a layer of synthetic resin
    • B32B27/06—Layered products comprising a layer of synthetic resin as the main or only constituent of a layer, which is next to another layer of the same or of a different material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de marquage d'acide nucléique au moyen de conjugués oligonucléotido-peptidiques à hybrider à l'acide nucléique. Elle porte aussi sur des méthodes de production de conjugués oligonucléotido-peptidiques, et sur des intermédiaires nucléotidiques de ces conjugués qui possèdent des groupes mercaptoéthyles protégés par des groupes butylthio. Ces intermédiaires peuvent former des conjugués oligonucléotido-peptidiques par une réaction avec un maléimido-peptide.
PCT/IB1999/001116 1998-06-01 1999-05-28 Methode de marquage d'acide nucleique WO1999063111A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39525/99A AU3952599A (en) 1998-06-01 1999-05-28 Method of labelling nucleic acid

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9811771.6A GB9811771D0 (fr) 1998-06-01 1998-06-01
GB9811771.6 1998-06-01
GB9813765.6 1998-06-25
GBGB9813765.6A GB9813765D0 (fr) 1998-06-25 1998-06-25

Publications (2)

Publication Number Publication Date
WO1999063111A2 WO1999063111A2 (fr) 1999-12-09
WO1999063111A3 true WO1999063111A3 (fr) 2000-02-24

Family

ID=26313778

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001116 WO1999063111A2 (fr) 1998-06-01 1999-05-28 Methode de marquage d'acide nucleique

Country Status (2)

Country Link
AU (1) AU3952599A (fr)
WO (1) WO1999063111A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160159834A1 (en) * 2013-04-22 2016-06-09 Northwestern University Alkyne phosphoramidites and preparation of spherical nucleic acid constructs
CN113512093A (zh) * 2020-04-10 2021-10-19 南京金斯瑞生物科技有限公司 一种合成含有二硫键和/或巯基修饰的多肽核酸偶联物的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000989A1 (fr) * 1990-07-10 1992-01-23 Imperial Chemical Industries Plc Procede de marquage non-isotopique d'acide nucleique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000989A1 (fr) * 1990-07-10 1992-01-23 Imperial Chemical Industries Plc Procede de marquage non-isotopique d'acide nucleique

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GOODWIN, JAY T. ET AL: "Incorporation of alkylthiol chains at C-5 of deoxyuridine", TETRAHEDRON LETT. (1993), 34(35), 5549-52, XP002123383 *
GOTTSCHLING, DIRK ET AL: "Synthesis of Oligodeoxyribonucleotides Containing N4-Mercaptoethylcytosin and Their Use in the Preparation of Oligonucleotide-Peptide Conjugates Carrying c-myc Tag-Sequence", BIOCONJUGATE CHEM. (1998), 9(6), 831-837, XP002123386 *
MAC MILLAN A. M. AND VERDINE G. L.: "Engineering tethered DNA molecules by the convertible nucleoside approach", TETRAHEDRON, vol. 47, no. 14/15, 1991, pages 2603 - 2616, XP002123382 *
OSBORNE, SCOTT E. ET AL: "Structure and Dynamics of Disulfide Cross-Linked DNA Triple Helixes", J. AM. CHEM. SOC. (1997), 119(6), 1171-1182, XP002123384 *
SUN, SENGEN ET AL: "Efficient Synthesis of 5-Thioalkyluridines via Ring Opening of.alpha.-Ureidomethylene Thiolactones", J. ORG. CHEM. (1996), 61(17), 5708-5709, XP002123385 *

Also Published As

Publication number Publication date
WO1999063111A2 (fr) 1999-12-09
AU3952599A (en) 1999-12-20

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