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WO1999001570A3 - Methods for accelerating hybridization of nucleic acid molecules - Google Patents

Methods for accelerating hybridization of nucleic acid molecules Download PDF

Info

Publication number
WO1999001570A3
WO1999001570A3 PCT/CA1998/000631 CA9800631W WO9901570A3 WO 1999001570 A3 WO1999001570 A3 WO 1999001570A3 CA 9800631 W CA9800631 W CA 9800631W WO 9901570 A3 WO9901570 A3 WO 9901570A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
methods
acid molecules
hybridization
Prior art date
Application number
PCT/CA1998/000631
Other languages
French (fr)
Other versions
WO1999001570A2 (en
Inventor
Robert N Bryan
Lynn P Cloney
Barbara A Farnworth
A Johanna Marostenmaki
Original Assignee
Id Biomedical Corp
Robert N Bryan
Lynn P Cloney
Barbara A Farnworth
A Johanna Marostenmaki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Id Biomedical Corp, Robert N Bryan, Lynn P Cloney, Barbara A Farnworth, A Johanna Marostenmaki filed Critical Id Biomedical Corp
Priority to AU83272/98A priority Critical patent/AU8327298A/en
Publication of WO1999001570A2 publication Critical patent/WO1999001570A2/en
Publication of WO1999001570A3 publication Critical patent/WO1999001570A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of increasing the hybridization rate of a first nucleic acid molecule to a second nucleic acid molecule is provided, comprising the step of mixing a first nucleic acid molecule, a second nucleic acid molecule, and polyamine and/or RNase H under conditions and for a time sufficient to permit hybridization of said first nucleic acid molecule to the second nucleic acid molecule.
PCT/CA1998/000631 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules WO1999001570A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU83272/98A AU8327298A (en) 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US5182797P 1997-07-03 1997-07-03
US60/051,827 1997-07-03
US8602198P 1998-05-18 1998-05-18
US60/086,021 1998-05-18
US9027398P 1998-06-22 1998-06-22
US60/090,273 1998-06-22

Publications (2)

Publication Number Publication Date
WO1999001570A2 WO1999001570A2 (en) 1999-01-14
WO1999001570A3 true WO1999001570A3 (en) 1999-08-05

Family

ID=27368023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1998/000631 WO1999001570A2 (en) 1997-07-03 1998-07-03 Methods for accelerating hybridization of nucleic acid molecules

Country Status (2)

Country Link
AU (1) AU8327298A (en)
WO (1) WO1999001570A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122314B2 (en) 2002-01-30 2006-10-17 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
EP3209675A4 (en) 2014-10-22 2018-04-18 Roka Bioscience, Inc. Compositions and methods for the detection of nucleic acids
US20190127783A1 (en) * 2016-04-27 2019-05-02 Prominex, Inc. Compositions and methods for the detection of nucleic acids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229442A1 (en) * 1986-01-07 1987-07-22 Gen-Probe Incorporated Accelerated nucleic acid reassociation method
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993001311A1 (en) * 1991-07-01 1993-01-21 Amersham International Plc Enhancement of polynucleotide hybridization
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
WO1997043450A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229442A1 (en) * 1986-01-07 1987-07-22 Gen-Probe Incorporated Accelerated nucleic acid reassociation method
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993001311A1 (en) * 1991-07-01 1993-01-21 Amersham International Plc Enhancement of polynucleotide hybridization
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
WO1997043450A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays

Also Published As

Publication number Publication date
WO1999001570A2 (en) 1999-01-14
AU8327298A (en) 1999-01-25

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