WO1999002566A3 - Proteine normalisee - Google Patents
Proteine normalisee Download PDFInfo
- Publication number
- WO1999002566A3 WO1999002566A3 PCT/EP1998/004237 EP9804237W WO9902566A3 WO 1999002566 A3 WO1999002566 A3 WO 1999002566A3 EP 9804237 W EP9804237 W EP 9804237W WO 9902566 A3 WO9902566 A3 WO 9902566A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- binding site
- standardised
- specific binding
- protein
- antibody constant
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 230000009870 specific binding Effects 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 230000027455 binding Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
L'invention concerne une protéine normalisée caractérisée en ce qu'elle présente au moins un site de liaison spécifique pour des enzymes, et/ou un site de liaison spécifique pour la région constante d'un anticorps et/ou un site de liaison spécifique qui comprend les fragments de régions constantes d'anticorps, le site de liaison étant fonctionnel après une séparation électrophorétique réalisée dans des conditions dénaturantes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19729248.8 | 1997-07-09 | ||
DE1997129248 DE19729248C1 (de) | 1997-07-09 | 1997-07-09 | Standardprotein |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999002566A2 WO1999002566A2 (fr) | 1999-01-21 |
WO1999002566A3 true WO1999002566A3 (fr) | 1999-04-01 |
Family
ID=7835079
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1998/004237 WO1999002566A2 (fr) | 1997-07-09 | 1998-07-08 | Proteine normalisee |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE19729248C1 (fr) |
WO (1) | WO1999002566A2 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998030684A1 (fr) | 1997-01-08 | 1998-07-16 | Life Technologies, Inc. | Procedes de production de proteines |
DE29900214U1 (de) * | 1999-01-09 | 1999-08-12 | Biosens Gesellschaft für Diagnostika mbH, 82041 Oberhaching | Western-Blot-Streifen mit Kontrollzonen |
DE10017526B4 (de) * | 1999-04-08 | 2013-05-29 | Jochen Uhlenküken | Standardprotein |
EP1054061A1 (fr) * | 1999-05-18 | 2000-11-22 | Jochen Uhlenküken | Standard protéique comprenant au moins un domaine liant les anticorps de la protéine G de Streptocoque |
IT1318400B1 (it) * | 2000-03-17 | 2003-08-25 | Dompe Spa | Markers di massa molecolare per westner blot. |
EP1236738A1 (fr) * | 2001-02-23 | 2002-09-04 | Jungbauer, Alois, Professor Dr. | Standard interne pour des procédés de séparation électrophorétiques et chromatographiques |
US7781173B2 (en) | 2003-09-25 | 2010-08-24 | Life Technologies Corporation | Homogeneous populations of molecules |
WO2005114220A2 (fr) * | 2004-05-17 | 2005-12-01 | Invitrogen Corporation | Compositions, trousses et methodes pour etalonnage en spectrometrie de masse |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4356072A (en) * | 1978-05-31 | 1982-10-26 | Oriental Yeast Company | Isoelectric point marker |
US4507233A (en) * | 1981-04-22 | 1985-03-26 | Oriental Yeast Co., Ltd. | Colored molecular weight marker |
WO1986006383A1 (fr) * | 1985-05-01 | 1986-11-06 | Commonwealth Serum Laboratories Commission | Determination du poids moleculaire de proteines |
EP0550771A1 (fr) * | 1991-07-25 | 1993-07-14 | Oriental Yeast Co., Ltd. | Proteine artificielle de combinaison avec les immunoglobulines |
US5506121A (en) * | 1992-11-03 | 1996-04-09 | Institut Fur Bioanalytik Gemeinnutzige Gesellschaft MBH | Fusion peptides with binding activity for streptavidin |
-
1997
- 1997-07-09 DE DE1997129248 patent/DE19729248C1/de not_active Expired - Fee Related
-
1998
- 1998-07-08 WO PCT/EP1998/004237 patent/WO1999002566A2/fr active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4356072A (en) * | 1978-05-31 | 1982-10-26 | Oriental Yeast Company | Isoelectric point marker |
US4507233A (en) * | 1981-04-22 | 1985-03-26 | Oriental Yeast Co., Ltd. | Colored molecular weight marker |
WO1986006383A1 (fr) * | 1985-05-01 | 1986-11-06 | Commonwealth Serum Laboratories Commission | Determination du poids moleculaire de proteines |
EP0550771A1 (fr) * | 1991-07-25 | 1993-07-14 | Oriental Yeast Co., Ltd. | Proteine artificielle de combinaison avec les immunoglobulines |
US5506121A (en) * | 1992-11-03 | 1996-04-09 | Institut Fur Bioanalytik Gemeinnutzige Gesellschaft MBH | Fusion peptides with binding activity for streptavidin |
Non-Patent Citations (3)
Title |
---|
LINDBLADH C. ET AL.: "STANDARD CALIBRATION PROTEINS FOR WESTERN BLOTTING OBTAINED BY GENETICALLY PREPARED PROTEIN A CONJUGATES", ANAL.BIOCHEM., vol. 197, 1991, pages 187 - 190, XP002087809 * |
SAMUEL D. ET AL.: "A SENSITIVE METHOD OF DETECTING PROTEINS ON DOT AND WESTERN BLOTS USING A MONOCLONAL ANTIBODY TO FITC", J.IMMUNOL.METHODS, vol. 107, 1988, pages 217 - 224, XP002087810 * |
ZUECO J. ET AL.: "PROTEIN A FUSION PROTEINS AS MOLECULAR WEIGHT MARKERS FOR USE IN IMMUNOBLOTTING", ANAL.BIOCHEM., vol. 207, 1992, pages 348 - 349, XP002087808 * |
Also Published As
Publication number | Publication date |
---|---|
DE19729248C1 (de) | 1998-07-09 |
WO1999002566A2 (fr) | 1999-01-21 |
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