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WO1999002566A3 - Proteine normalisee - Google Patents

Proteine normalisee Download PDF

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Publication number
WO1999002566A3
WO1999002566A3 PCT/EP1998/004237 EP9804237W WO9902566A3 WO 1999002566 A3 WO1999002566 A3 WO 1999002566A3 EP 9804237 W EP9804237 W EP 9804237W WO 9902566 A3 WO9902566 A3 WO 9902566A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding site
standardised
specific binding
protein
antibody constant
Prior art date
Application number
PCT/EP1998/004237
Other languages
German (de)
English (en)
Other versions
WO1999002566A2 (fr
Inventor
Jochen Uhlenkueken
Gerd Schmidt
Manfred Lansing
Original Assignee
Jochen Uhlenkueken
Gerd Schmidt
Manfred Lansing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jochen Uhlenkueken, Gerd Schmidt, Manfred Lansing filed Critical Jochen Uhlenkueken
Publication of WO1999002566A2 publication Critical patent/WO1999002566A2/fr
Publication of WO1999002566A3 publication Critical patent/WO1999002566A3/fr

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une protéine normalisée caractérisée en ce qu'elle présente au moins un site de liaison spécifique pour des enzymes, et/ou un site de liaison spécifique pour la région constante d'un anticorps et/ou un site de liaison spécifique qui comprend les fragments de régions constantes d'anticorps, le site de liaison étant fonctionnel après une séparation électrophorétique réalisée dans des conditions dénaturantes.
PCT/EP1998/004237 1997-07-09 1998-07-08 Proteine normalisee WO1999002566A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19729248.8 1997-07-09
DE1997129248 DE19729248C1 (de) 1997-07-09 1997-07-09 Standardprotein

Publications (2)

Publication Number Publication Date
WO1999002566A2 WO1999002566A2 (fr) 1999-01-21
WO1999002566A3 true WO1999002566A3 (fr) 1999-04-01

Family

ID=7835079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/004237 WO1999002566A2 (fr) 1997-07-09 1998-07-08 Proteine normalisee

Country Status (2)

Country Link
DE (1) DE19729248C1 (fr)
WO (1) WO1999002566A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030684A1 (fr) 1997-01-08 1998-07-16 Life Technologies, Inc. Procedes de production de proteines
DE29900214U1 (de) * 1999-01-09 1999-08-12 Biosens Gesellschaft für Diagnostika mbH, 82041 Oberhaching Western-Blot-Streifen mit Kontrollzonen
DE10017526B4 (de) * 1999-04-08 2013-05-29 Jochen Uhlenküken Standardprotein
EP1054061A1 (fr) * 1999-05-18 2000-11-22 Jochen Uhlenküken Standard protéique comprenant au moins un domaine liant les anticorps de la protéine G de Streptocoque
IT1318400B1 (it) * 2000-03-17 2003-08-25 Dompe Spa Markers di massa molecolare per westner blot.
EP1236738A1 (fr) * 2001-02-23 2002-09-04 Jungbauer, Alois, Professor Dr. Standard interne pour des procédés de séparation électrophorétiques et chromatographiques
US7781173B2 (en) 2003-09-25 2010-08-24 Life Technologies Corporation Homogeneous populations of molecules
WO2005114220A2 (fr) * 2004-05-17 2005-12-01 Invitrogen Corporation Compositions, trousses et methodes pour etalonnage en spectrometrie de masse

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4356072A (en) * 1978-05-31 1982-10-26 Oriental Yeast Company Isoelectric point marker
US4507233A (en) * 1981-04-22 1985-03-26 Oriental Yeast Co., Ltd. Colored molecular weight marker
WO1986006383A1 (fr) * 1985-05-01 1986-11-06 Commonwealth Serum Laboratories Commission Determination du poids moleculaire de proteines
EP0550771A1 (fr) * 1991-07-25 1993-07-14 Oriental Yeast Co., Ltd. Proteine artificielle de combinaison avec les immunoglobulines
US5506121A (en) * 1992-11-03 1996-04-09 Institut Fur Bioanalytik Gemeinnutzige Gesellschaft MBH Fusion peptides with binding activity for streptavidin

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4356072A (en) * 1978-05-31 1982-10-26 Oriental Yeast Company Isoelectric point marker
US4507233A (en) * 1981-04-22 1985-03-26 Oriental Yeast Co., Ltd. Colored molecular weight marker
WO1986006383A1 (fr) * 1985-05-01 1986-11-06 Commonwealth Serum Laboratories Commission Determination du poids moleculaire de proteines
EP0550771A1 (fr) * 1991-07-25 1993-07-14 Oriental Yeast Co., Ltd. Proteine artificielle de combinaison avec les immunoglobulines
US5506121A (en) * 1992-11-03 1996-04-09 Institut Fur Bioanalytik Gemeinnutzige Gesellschaft MBH Fusion peptides with binding activity for streptavidin

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LINDBLADH C. ET AL.: "STANDARD CALIBRATION PROTEINS FOR WESTERN BLOTTING OBTAINED BY GENETICALLY PREPARED PROTEIN A CONJUGATES", ANAL.BIOCHEM., vol. 197, 1991, pages 187 - 190, XP002087809 *
SAMUEL D. ET AL.: "A SENSITIVE METHOD OF DETECTING PROTEINS ON DOT AND WESTERN BLOTS USING A MONOCLONAL ANTIBODY TO FITC", J.IMMUNOL.METHODS, vol. 107, 1988, pages 217 - 224, XP002087810 *
ZUECO J. ET AL.: "PROTEIN A FUSION PROTEINS AS MOLECULAR WEIGHT MARKERS FOR USE IN IMMUNOBLOTTING", ANAL.BIOCHEM., vol. 207, 1992, pages 348 - 349, XP002087808 *

Also Published As

Publication number Publication date
DE19729248C1 (de) 1998-07-09
WO1999002566A2 (fr) 1999-01-21

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