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WO1999011823A3 - Selection de paires d'amorces pcr destinees a l'amplification d'un groupe de sequences nucleotidiques - Google Patents

Selection de paires d'amorces pcr destinees a l'amplification d'un groupe de sequences nucleotidiques Download PDF

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Publication number
WO1999011823A3
WO1999011823A3 PCT/US1998/018392 US9818392W WO9911823A3 WO 1999011823 A3 WO1999011823 A3 WO 1999011823A3 US 9818392 W US9818392 W US 9818392W WO 9911823 A3 WO9911823 A3 WO 9911823A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequences
group
primer pairs
amplify
provides
Prior art date
Application number
PCT/US1998/018392
Other languages
English (en)
Other versions
WO1999011823A2 (fr
Inventor
Michael Mcclelland
Graziano Pesole
Original Assignee
Sidney Kimmel Cancer Ct
Michael Mcclelland
Graziano Pesole
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sidney Kimmel Cancer Ct, Michael Mcclelland, Graziano Pesole filed Critical Sidney Kimmel Cancer Ct
Priority to EP98945882A priority Critical patent/EP1007739A2/fr
Priority to AU93027/98A priority patent/AU9302798A/en
Publication of WO1999011823A2 publication Critical patent/WO1999011823A2/fr
Publication of WO1999011823A3 publication Critical patent/WO1999011823A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une méthode permettant de déterminer un ensemble de paires d'amorces destinées à l'amplification d'un groupe de séquences nucléotidiques associées. La méthode de l'invention consiste à identifier un groupe de séquences nucléotidiques associées; à produire l'ensemble d'amorces correspondant à chacune des séquences nucléotidiques associées; à déterminer pour chaque appariement systématique de chacune des amorces quelles sont les séquences nucléotidiques associées amplifiées; et à sélectionner à partir des appariements systématiques, un sous-ensemble amplifiant toutes les séquences nucléotidiques associées. L'invention concerne également une méthode d'utilisation d'un ensemble de paires d'amorces, méthode qui consiste à amplifier un groupe de séquences nucléotidiques associées pour identifier des séquences nucléotidiques associées au groupe de séquences nucléotidiques d'origine. L'invention concerne aussi un dispositif informatique permettant de réaliser les différentes étapes, exécutées de manière informatisée, de l'invention. L'invention concerne enfin un logiciel comprenant un milieu vecteur de signaux permettant de mettre en oeuvre la méthode précitée.
PCT/US1998/018392 1997-09-05 1998-09-04 Selection de paires d'amorces pcr destinees a l'amplification d'un groupe de sequences nucleotidiques WO1999011823A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98945882A EP1007739A2 (fr) 1997-09-05 1998-09-04 Selection de paires d'amorces pcr destinees a l'amplification d'un groupe de sequences nucleotidiques
AU93027/98A AU9302798A (en) 1997-09-05 1998-09-04 Selection of pcr primer pairs to amplify a group of nucleotide sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92581697A 1997-09-05 1997-09-05
US08/925,816 1997-09-05

Publications (2)

Publication Number Publication Date
WO1999011823A2 WO1999011823A2 (fr) 1999-03-11
WO1999011823A3 true WO1999011823A3 (fr) 1999-06-10

Family

ID=25452285

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/018392 WO1999011823A2 (fr) 1997-09-05 1998-09-04 Selection de paires d'amorces pcr destinees a l'amplification d'un groupe de sequences nucleotidiques

Country Status (3)

Country Link
EP (1) EP1007739A2 (fr)
AU (1) AU9302798A (fr)
WO (1) WO1999011823A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
US6898531B2 (en) 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
US6740510B2 (en) 2001-09-05 2004-05-25 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
US20030108919A1 (en) * 2001-09-05 2003-06-12 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
JP5409005B2 (ja) * 2005-10-27 2014-02-05 ロゼッタ インファーマティックス エルエルシー 非ランダムプライマーを用いる核酸増幅
WO2007067907A1 (fr) 2005-12-06 2007-06-14 Ambion, Inc. Amorces de transcription inverse et procedes de conception
EP2209912A1 (fr) * 2007-10-26 2010-07-28 Rosetta Inpharmatics LLC Synthèse d'adnc utilisant des amorces non aléatoires
US20100159533A1 (en) * 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
WO2012032510A1 (fr) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Amorces pour l'amplification d'adn et procédés de sélection de ces dernières

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014844A1 (fr) * 1991-02-25 1992-09-03 California Institute Of Biological Research Procede d'amplification enzymatique du genome amorce par une sequence consensuelle permettant de prendre l'empreinte de genomes
WO1993018176A1 (fr) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute Procedes de clonage d'arn messager
WO1993024655A1 (fr) * 1992-05-27 1993-12-09 Amersham International Plc Analyse d'arn

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014844A1 (fr) * 1991-02-25 1992-09-03 California Institute Of Biological Research Procede d'amplification enzymatique du genome amorce par une sequence consensuelle permettant de prendre l'empreinte de genomes
WO1993018176A1 (fr) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute Procedes de clonage d'arn messager
WO1993024655A1 (fr) * 1992-05-27 1993-12-09 Amersham International Plc Analyse d'arn

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
JOHNSTON S L ET AL: "A NOVEL METHOD FOR SEQUENCING MEMBERS OF MULTI-GENE FAMILIES", NUCLEIC ACIDS RESEARCH, vol. 23, no. 15, August 1995 (1995-08-01), pages 3074/3075, XP002911090 *
LIANG P ET AL: "DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER RNA BY MEANS OF THE POLYMERASE CHAIN REACTION", SCIENCE, vol. 257, no. 5072, 14 August 1992 (1992-08-14), pages 967 - 971, XP000508268 *
LIBERT F ET AL.: "Selective amplification and cloning of four new members of the G protein-coupled receptor family", SCIENCE, vol. 244, 1989, pages 569 - 572, XP002090068 *
LOPEZ-NIETO C E AND NIGAM S K: "Selective amplificationof protein-coding regions of large sets of genes using statistically designed primer sets", NATURE BIOTECHNOLOGY, vol. 14, 1996, XP002090066 *
LOWE T ET AL.: "A computer program for selection of oligonucleotide primers for polymerase chain reaction", NUCLEIC ACIDS RESEARCH, vol. 18, no. 7, 1990, pages 1757 - 1761, XP002090070 *
PESOLE G ET AL.: "GeneUp: a program to select short PCR primer pairs that occur in multiple members of sequence lists", BIOTECHNIQUES, vol. 25, no. 1, 1998, pages 112 - 117, XP002090071 *
PESOLE G ET AL.: "WORDUP: an efficient algorithm for discovering statistically significant patterns in DNA sequences", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, pages 2871 - 2875, XP002090067 *
STONE B AND WHARTON W: "Targeted RNA fingerprinting: The cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family", NUCLEIC ACIDS RESEARCH, vol. 22, no. 13, 1994, pages 2612 - 2618, XP002090069 *

Also Published As

Publication number Publication date
WO1999011823A2 (fr) 1999-03-11
AU9302798A (en) 1999-03-22
EP1007739A2 (fr) 2000-06-14

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