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WO1999033867A3 - Modified nucleic acid analogues - Google Patents

Modified nucleic acid analogues Download PDF

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Publication number
WO1999033867A3
WO1999033867A3 PCT/EP1998/008317 EP9808317W WO9933867A3 WO 1999033867 A3 WO1999033867 A3 WO 1999033867A3 EP 9808317 W EP9808317 W EP 9808317W WO 9933867 A3 WO9933867 A3 WO 9933867A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligomers
nucleic acid
modified nucleic
acid analogues
low
Prior art date
Application number
PCT/EP1998/008317
Other languages
German (de)
French (fr)
Other versions
WO1999033867A2 (en
Inventor
Frank Bergmann
Der Eltz Herbert Von
Rupert Herrmann
Stefanie Koehler
Volker Schlueter
Original Assignee
Roche Diagnostics Gmbh
Frank Bergmann
Der Eltz Herbert Von
Rupert Herrmann
Stefanie Koehler
Volker Schlueter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Frank Bergmann, Der Eltz Herbert Von, Rupert Herrmann, Stefanie Koehler, Volker Schlueter filed Critical Roche Diagnostics Gmbh
Priority to EP98966833A priority Critical patent/EP0996631A1/en
Publication of WO1999033867A2 publication Critical patent/WO1999033867A2/en
Publication of WO1999033867A3 publication Critical patent/WO1999033867A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Oligomers comprising at least one non-nucleoside unit containing an acid function with a pKa that is lower than 3.0 are particularly suitable as detection probes for nucleic acids as non-specific interactions are reduced in comparison with unmodified oligomers. Even when the concentration of hybridisation buffers is low, it is possible to obtain high signal dynamics and high sensitivity for a low blank signal. Said oligomers are also suitable as primers for lengthening reactions using polymerases and for transfection.
PCT/EP1998/008317 1997-12-23 1998-12-18 Modified nucleic acid analogues WO1999033867A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98966833A EP0996631A1 (en) 1997-12-23 1998-12-18 Modified nucleic acid analogues

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19757516A DE19757516A1 (en) 1997-12-23 1997-12-23 New oligo-nucleotide analogs with negatively charged non-nucleoside units
DE19757516.1 1997-12-23

Publications (2)

Publication Number Publication Date
WO1999033867A2 WO1999033867A2 (en) 1999-07-08
WO1999033867A3 true WO1999033867A3 (en) 1999-09-02

Family

ID=7853170

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/008317 WO1999033867A2 (en) 1997-12-23 1998-12-18 Modified nucleic acid analogues

Country Status (3)

Country Link
EP (1) EP0996631A1 (en)
DE (1) DE19757516A1 (en)
WO (1) WO1999033867A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10019136A1 (en) * 2000-04-18 2001-10-31 Aventis Pharma Gmbh Polyamide nucleic acid derivatives, agents and processes for their preparation
DE10019135A1 (en) 2000-04-18 2001-10-31 Aventis Pharma Gmbh Polyamide nucleic acid derivatives, agents and processes for their preparation
EP1251183A3 (en) * 2001-04-18 2003-12-10 Exiqon A/S Improved helper probes for detection of a target sequence by a capture oligonucleotide

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
WO1996011205A1 (en) * 1994-10-06 1996-04-18 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
WO1996035705A1 (en) * 1995-05-10 1996-11-14 Isis Pharmaceuticals, Inc. Inhibition of transcription factor-mediated transcriptional activation by oligomer strand invasion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
WO1996011205A1 (en) * 1994-10-06 1996-04-18 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
WO1996035705A1 (en) * 1995-05-10 1996-11-14 Isis Pharmaceuticals, Inc. Inhibition of transcription factor-mediated transcriptional activation by oligomer strand invasion

Also Published As

Publication number Publication date
WO1999033867A2 (en) 1999-07-08
EP0996631A1 (en) 2000-05-03
DE19757516A1 (en) 1999-06-24

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