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WO1999034003A3 - Proteases provenant d'organismes a gram positif - Google Patents

Proteases provenant d'organismes a gram positif Download PDF

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Publication number
WO1999034003A3
WO1999034003A3 PCT/US1998/027020 US9827020W WO9934003A3 WO 1999034003 A3 WO1999034003 A3 WO 1999034003A3 US 9827020 W US9827020 W US 9827020W WO 9934003 A3 WO9934003 A3 WO 9934003A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Application number
PCT/US1998/027020
Other languages
English (en)
Other versions
WO1999034003A2 (fr
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to US09/554,998 priority Critical patent/US6599731B1/en
Priority to AU19301/99A priority patent/AU1930199A/en
Priority to EP98964108A priority patent/EP1042490A2/fr
Publication of WO1999034003A2 publication Critical patent/WO1999034003A2/fr
Publication of WO1999034003A3 publication Critical patent/WO1999034003A3/fr
Priority to US11/013,992 priority patent/US20050101504A1/en
Priority to US11/014,361 priority patent/US7098021B2/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne l'identification d'une nouvelle métalloprotéase dans des micro-organismes à Gram positif. Elle concerne également les séquences d'acides nucléiques et d'acides aminés de cette métalloprotéase; des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant la métalloprotéase; des cellules hôtes contenant, de plus, un acide nucléique codant des protéines hétérologues souhaitées, telles que des enzymes; des compositions de nettoyage, des aliments pour animaux et des compositions utilisées pour traiter un textile, qui contiennent cette métalloprotéase.
PCT/US1998/027020 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif WO1999034003A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/554,998 US6599731B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
AU19301/99A AU1930199A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98964108A EP1042490A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif
US11/013,992 US20050101504A1 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms
US11/014,361 US7098021B2 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727464.1A GB9727464D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727464.1 1997-12-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09554998 A-371-Of-International 1998-12-17
US10/460,718 Division US6872807B2 (en) 1997-12-30 2003-06-12 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034003A2 WO1999034003A2 (fr) 1999-07-08
WO1999034003A3 true WO1999034003A3 (fr) 1999-11-18

Family

ID=10824308

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027020 WO1999034003A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif

Country Status (4)

Country Link
EP (1) EP1042490A2 (fr)
AU (1) AU1930199A (fr)
GB (1) GB9727464D0 (fr)
WO (1) WO1999034003A2 (fr)

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GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
AU2002347019A1 (en) 2001-12-07 2003-06-17 Novozymes A/S Polypeptides having protease activity and nucleic acids encoding same
WO2010097436A1 (fr) * 2009-02-27 2010-09-02 Novozymes A/S Cellules mutantes à expression réduite de la métallopeptidase, appropriées pour la production de polypeptides recombinés
TR201906371T4 (tr) 2013-12-13 2019-05-21 Danisco Inc Bacillus türlerinin serin proteazları.
DK3553173T3 (da) 2013-12-13 2021-08-23 Danisco Us Inc Serinproteaser af bacillus gibsonii-clade
EP3083704B1 (fr) 2013-12-16 2022-08-17 Nutrition & Biosciences USA 4, Inc. Utilisation de poly(éthers d'alpha-1,3-glucane) en tant que modificateurs de viscosité
JP6559139B2 (ja) 2013-12-18 2019-08-14 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company カチオン性ポリα−1,3−グルカンエーテル
EP3105256A1 (fr) 2014-02-14 2016-12-21 E. I. du Pont de Nemours and Company Poly-alpha-1,3-1,6-glucanes utilisables en vue de la modification de la viscosité
CN106132997A (zh) 2014-03-11 2016-11-16 纳幕尔杜邦公司 作为洗涤剂助洗剂的氧化的聚α‑1,3‑葡聚糖
EP3119884B1 (fr) 2014-03-21 2019-07-10 Danisco US Inc. Sérine-protéases de l'espèce bacillus
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3919599A1 (fr) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions contenant un ou plusieurs composés d'éther de poly alpha-1,3-glucane
WO2016061438A1 (fr) 2014-10-17 2016-04-21 Danisco Us Inc. Sérines protéases de l'espèce bacillus
EP3212783B1 (fr) 2014-10-27 2024-06-26 Danisco US Inc. Sérines protéases
EP3212782B1 (fr) 2014-10-27 2019-04-17 Danisco US Inc. Sérine protéases
WO2016069552A1 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérines protéases
WO2016069557A1 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérine-protéases de l'espèce bacillus
WO2016069548A2 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérine-protéases
AU2015369967B2 (en) 2014-12-23 2019-06-27 Nutrition & Biosciences USA 4, Inc. Enzymatically produced cellulose
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
CN107849549B (zh) 2015-06-17 2024-04-05 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
WO2017079756A1 (fr) 2015-11-05 2017-05-11 Danisco Us Inc Mannanases de paenibacillus et bacillus spp.
JP7364331B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス(Paenibacillus)属種マンナナーゼ
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (fr) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
EP3374401B1 (fr) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
WO2017106676A1 (fr) 2015-12-18 2017-06-22 Danisco Us Inc Polypeptides ayant une activité endoglucanase et leurs utilisations
CN109477112B (zh) 2016-05-31 2024-09-06 丹尼斯科美国公司 蛋白酶变体及其用途
JP7152319B2 (ja) 2016-06-17 2022-10-12 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
EP3535365A2 (fr) 2016-11-07 2019-09-11 Danisco US Inc. Composition détergente pour le linge
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
EP4212622A3 (fr) 2016-12-21 2023-11-29 Danisco US Inc. Sérine protéases du clade de bacillus gibsonii
EP3583210B1 (fr) 2017-03-15 2021-07-07 Danisco US Inc. Sérine protéases de type trypsine et leurs utilisations
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
WO2019245704A1 (fr) 2018-06-19 2019-12-26 Danisco Us Inc Variantes de subtilisine
US20210363470A1 (en) 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
EP3856882A1 (fr) 2018-09-27 2021-08-04 Danisco US Inc. Compositions pour nettoyage d'instrument médical
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US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
CN114174486A (zh) 2019-06-06 2022-03-11 丹尼斯科美国公司 用于清洁的方法和组合物
CN116323935A (zh) 2020-08-27 2023-06-23 丹尼斯科美国公司 用于清洁的酶和酶组合物
WO2022165107A1 (fr) 2021-01-29 2022-08-04 Danisco Us Inc Compositions pour le nettoyage et procédés associés
CN117616120A (zh) 2021-06-30 2024-02-27 丹尼斯科美国公司 变体脂肪酶及其用途
WO2023034486A2 (fr) 2021-09-03 2023-03-09 Danisco Us Inc. Compositions de blanchisserie pour le nettoyage
EP4448749A2 (fr) 2021-12-16 2024-10-23 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
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EP4448750A2 (fr) 2021-12-16 2024-10-23 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
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WO2024191711A1 (fr) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Extraits fermentés de brevibacillus pour le nettoyage et la lutte contre les mauvaises odeurs et leur utilisation
WO2025071996A1 (fr) 2023-09-28 2025-04-03 Danisco Us Inc. Variant d'enzymes cutinases à solubilité améliorée et leurs utilisations
WO2025085351A1 (fr) 2023-10-20 2025-04-24 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation

Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106131, Database accession no. Z99120 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106132, Database accession no. G70017 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
WO1999034003A2 (fr) 1999-07-08
AU1930199A (en) 1999-07-19
EP1042490A2 (fr) 2000-10-11
GB9727464D0 (en) 1998-02-25

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