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WO1999034220A3 - Real-time monitoring of an analyte by chromatography using an on-line assay - Google Patents

Real-time monitoring of an analyte by chromatography using an on-line assay Download PDF

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Publication number
WO1999034220A3
WO1999034220A3 PCT/US1998/026172 US9826172W WO9934220A3 WO 1999034220 A3 WO1999034220 A3 WO 1999034220A3 US 9826172 W US9826172 W US 9826172W WO 9934220 A3 WO9934220 A3 WO 9934220A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
analyte
culture fluid
protein
loading
Prior art date
Application number
PCT/US1998/026172
Other languages
French (fr)
Other versions
WO1999034220A2 (en
Inventor
Gregory S Blank
Robert Lee Fahrner
Martin Vanderlaan
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to EP98965982A priority Critical patent/EP1044378A2/en
Priority to AU21990/99A priority patent/AU2199099A/en
Publication of WO1999034220A2 publication Critical patent/WO1999034220A2/en
Publication of WO1999034220A3 publication Critical patent/WO1999034220A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The presence of a contaminating analyte in a protein mixture may obscure detection of a protein of interest. In addition, antibody breakthrough is obscured by flow-through of contaminant proteins when loading cell culture fluid onto affinity columns. An on-line chromatographic assay described herein allows real-time measurement of contaminating analyte during purification or antibody breakthrough during loading and collection of a protein of interest in preparative chromatography. Breakthrough curves are generated allowing the user to end loading of cell culture fluid and/or to end effluent collection for maximization of efficient purification and preventing waste of cell culture fluid.
PCT/US1998/026172 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay WO1999034220A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98965982A EP1044378A2 (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay
AU21990/99A AU2199099A (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7015097P 1997-12-31 1997-12-31
US60/070,150 1997-12-31

Publications (2)

Publication Number Publication Date
WO1999034220A2 WO1999034220A2 (en) 1999-07-08
WO1999034220A3 true WO1999034220A3 (en) 1999-09-02

Family

ID=22093458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026172 WO1999034220A2 (en) 1997-12-31 1998-12-09 Real-time monitoring of an analyte by chromatography using an on-line assay

Country Status (3)

Country Link
EP (1) EP1044378A2 (en)
AU (1) AU2199099A (en)
WO (1) WO1999034220A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353440A (en) * 2015-07-13 2017-01-25 帕尔公司 Optimizing operating binding capacity for a multiple column chromatography process

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395538B1 (en) 1999-07-16 2002-05-28 Human Genome Sciences, Inc. Method and system for providing real-time, in situ biomanufacturing process monitoring and control in response to IR spectroscopy
US8029743B2 (en) 2007-09-19 2011-10-04 General Electric Company Microfluidic device with vertical injection aperture
US7740747B2 (en) 2007-12-28 2010-06-22 General Electric Company Injection method for microfluidic chips
US7740748B2 (en) 2008-10-27 2010-06-22 General Electric Company Electrophoresis system and method
US7927476B2 (en) 2008-12-22 2011-04-19 General Electric Company Injection method for microfluidic chips
DE102014010353B4 (en) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Device, use of this device and method for substance separation with improved utilization of the capacity of chromatographic media
IL290358B2 (en) * 2016-04-14 2024-03-01 Lonza Ag Compositions and methods for the detection of host cell proteins
CN111902720B (en) 2018-03-21 2025-02-07 沃特世科技公司 Non-antibody high affinity based sample preparation, adsorbents, devices and methods

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993007168A2 (en) * 1991-09-30 1993-04-15 Perseptive Biosystems, Inc. Protein chromatography system
US5234586A (en) * 1991-03-28 1993-08-10 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
WO1993020449A1 (en) * 1992-03-27 1993-10-14 Perseptive Biosystems, Inc. Rapid hypersensitive flowthrough immunodetection system
WO1996040398A1 (en) * 1995-06-07 1996-12-19 Biosepra Inc. On line detection of a desired solute in an effluent stream using fluorescence spectroscopy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234586A (en) * 1991-03-28 1993-08-10 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
WO1993007168A2 (en) * 1991-09-30 1993-04-15 Perseptive Biosystems, Inc. Protein chromatography system
WO1993020449A1 (en) * 1992-03-27 1993-10-14 Perseptive Biosystems, Inc. Rapid hypersensitive flowthrough immunodetection system
WO1996040398A1 (en) * 1995-06-07 1996-12-19 Biosepra Inc. On line detection of a desired solute in an effluent stream using fluorescence spectroscopy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
H. A. CHASE: "Rapid chromatographic monitoring of bioprocesses", BIOSENSORS, vol. 2, 1986, ISSN 0265-928X, pages 269 - 286, XP002105368 *
LEVISON P R ET AL: "Influence of flow-rate on the chromatographic performance of agarose- and cellulose-based anion-exchange media", JOURNAL OF CHROMATOGRAPHY A, vol. 734, no. 1, 17 May 1996 (1996-05-17), pages 137-143, XP004039088 *
N.B. AFEYAN: "Automated real-time immunoassay of biomolecules", NATURE., vol. 358, 13 August 1992 (1992-08-13), LONDON GB, pages 603 - 604, XP002105367 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353440A (en) * 2015-07-13 2017-01-25 帕尔公司 Optimizing operating binding capacity for a multiple column chromatography process

Also Published As

Publication number Publication date
WO1999034220A2 (en) 1999-07-08
AU2199099A (en) 1999-07-19
EP1044378A2 (en) 2000-10-18

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