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WO2000053843A1 - Procede et preparation enzymatique a activite de sterylesterase permettant de controler la poix pendant la fabrication de papier - Google Patents

Procede et preparation enzymatique a activite de sterylesterase permettant de controler la poix pendant la fabrication de papier Download PDF

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Publication number
WO2000053843A1
WO2000053843A1 PCT/FI2000/000180 FI0000180W WO0053843A1 WO 2000053843 A1 WO2000053843 A1 WO 2000053843A1 FI 0000180 W FI0000180 W FI 0000180W WO 0053843 A1 WO0053843 A1 WO 0053843A1
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Prior art keywords
enzyme preparation
steryl
trichoderma
vtt
activity
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PCT/FI2000/000180
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English (en)
Inventor
Johanna Buchert
Annikka Mustranta
Hanna Vuorentaso
Peter Spetz
Rainer Ekman
Bjarne Holmbom
Original Assignee
Valtion Teknillinen Tutkimuskeskus
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Priority to AU32936/00A priority Critical patent/AU3293600A/en
Publication of WO2000053843A1 publication Critical patent/WO2000053843A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
    • D21C9/08—Removal of fats, resins, pitch or waxes; Chemical or physical purification, i.e. refining, of crude cellulose by removing non-cellulosic contaminants, optionally combined with bleaching

Definitions

  • the present invention relates to the control of extractives during paper manufacture.
  • the invention concerns a process for enzymatically hydrolyzing steryl esters in pulp suspensions or in the process waters.
  • This invention provides also a novel enzyme composition capable of hydrolyzing the ester linkages in steryl esters.
  • the extractives of wood are a very heterogenous group of compounds.
  • the extractives are partially dispersed in process waters during pulping, bleaching and paper manufacture.
  • a part of the lipophilic extractives are in the form of colloidal droplets, which can further form aggregates and which, when adhering to surfaces on paper machines can cause breakages of the paper web in the paper manufacture.
  • the extractives can also cause sticky spots in the paper, resulting in impaired paper product quality. Sticky spots in the paper give problems with the runnability of the paper machine and in further coating and printing operations.
  • the amount and composition of wood extractives vary greatly.
  • the amount of extractives in spruce wood is about half of that in pine and birch.
  • About 30 % of the extractives of softwoods are triglycerides, 20 % are steryl esters and 25 % resin acids ( Sj ⁇ str ⁇ m, 1981).
  • the composition of birch extractives is totally different from the composition of the extractives of softwood. There are no resin acids in birch, and the relative amounts of neutral components and fatty acid esters are in birch clearly more abundant than in pine or spruce.
  • the amount of extractives in softwood pulp cooked by the alkaline kraft method is usually less than 0.1 %.
  • the high pH of the cooking process dissolves and disperses the extractives, which are liberated to the washing water in washing stage.
  • Triglycerides and part of the steryl esters contained in the extractives are hydrolyzed at alkaline cooking conditions.
  • the amount of extractives of birch and aspen kraft pulp is usually 0.3 to 0.8 %.
  • the reason for this high extractive content is the poor dissolution and dispersion of the birch extractives during the cooking process.
  • the steryl esters that remain unhydrolyzed are particularly difficult to remove from the pulp.
  • Some steryl esters, such as esters of 4,4-dimethyl sterols present especially in hardwoods are very stable and resist alkaline hydrolysis even in kraft cooking, and thus their relative amount increases in the extractives remaining in kraft pulp.
  • Resinase treatment results in hydrolysis of triglycerides present in the extractives (Mustranta et al, 1998).
  • the pitch control with resinase has resulted in better and steady operations and improved product quality (Fujita et al. 1992).
  • the problem of the resinase treatment is its limitation to triglycerides, which account only for about 30 - 40 % of the extractives.
  • Resinase lipases hydrolyze triglycerides by liberating fatty acids and glycerol.
  • thermostable lipase suggests the addition of thermostable lipase to white-water or to pulp.
  • Leone et al. (1998) have also disclosed the screening of fungi capable of growing on aspen steryl esters/waxes. The ability of fungi to consume steryl esters/waxes was assessed in the presence of other carbon sources including glucose and triglycerides. The best consumers of steryl esters/waxes were Aspergillus luchuensis, Phanerochaete chrysosporium and Cunninghamella elegans. However, Leone et al. did not isolate or purify any enzymes, which would be responsible of the steryl ester and/or wax consumption.
  • mechanical, chemimechanical or chemical pulps can be treated by the enzymatic method of this invention.
  • the enzyme compositions of this invention can be added to the pulp suspensions in connection with mechanical, chemimechanical or chemical pulping or before, during or after bleaching or at any stage of the paper manufacturing process before the paper machine.
  • the process waters and/or pulp suspensions are preferably treated in the first stages of the manufacture of wood-containing paper and board i.e. the process waters circulated in mechanical or chemi -mechanical pulping processes and/or pulp suspensions from these processes. It is advantageous to treat the process waters and/or pulp suspensions from mechanical or chemimechanical pulping, because the steryl esters remain nearly unchanged in the mentioned processes. The hydrophilicity of steryl esters is increased and thus their tendency to form aggregates and adhere to machine parts during the subsequent processing steps of the paper or board manufacturing process is decreased.
  • pulp suspensions and/or process waters from chemical pulping are treated with the enzyme preparations of this invention, because especially hardwood comprises very stable steryl esterases which resist alkaline hydrolysis in kraft cooking. It is also advantageous to treat sulphite pulp made from hardwood or softwood and/or process waters originating from these processes by the enzymatic method of this invention.
  • the enzyme treatment can be carried out at the head box, where the different components of paper (or board) are mixed together.
  • Wood extractives from various pulping processes are dispersed as colloidal droplets to the process waters. Especially, if the process conditions (pH, temperature, amount of calcium) are changed the colloidal droplets form aggregates, which adhere to the surfaces on paper machines. When fixed to pulp the aggregates are less harmful than when present in the process waters. This is due to the potential adhesion of the aggregates to machine or wire parts. Accordingly it is most advantageous to treat process waters by the enzymatic method of this invention.
  • the consistency of pulp is usually 5 % to 35 %, or typically 10 % to 20 %.
  • the consistency of the pulp suspension to be treated by the method of this invention is preferably 5 % or less, more preferably 3 %, or less, still more preferably 2 % or less and most preferably 1 % or less.
  • the consistency of the pulp suspension at the head box of a paper machine is about 1 %. In process waters the consistency is typically
  • This invention provides also new enzyme preparations capable of efficiently degrading the steryl ester linkages in steryl esters of wood extractives.
  • These enzyme preparations are derived preferably from strains belonging to Trichoderma, Paecilomyces,
  • Rhizomucor Candida, Bacillus or Pseudomonas, most preferably from Trichoderma, Paecilomyces, Rhizomucor, Candida or Pseudomonas.
  • the strains belong preferably to the species Trichoderma reesei, Trichoderma longibrachiatum, Trichoderma citrinoviride, Paecilomyces variotii, Rhizomucor miehei, Candida tropicalis, Bacillus pumilus, Bacillus subtilis/macerans, Bacillus subtilis or Pseudomonas stutzeri.
  • Trichoderma reesei Trichoderma longibrachiatum, Trichoderma citrinoviride, Paecilomyces variotii, Rhizomucor miehei, Candida tropicalis, Bacillus pumilus, Bacillus subtilis/macerans, Bacillus subtilis or Pseudomonas stutzeri.
  • the gene or genes encoding an enzyme or enzymes capable of degrading the steryl ester linkages in steryl esters of wood extractives.
  • the gene is then transfered to a suitable host, preferably under the regulation of a strong promoter, making capable of an efficient production of the desired enzyme product.
  • This invention thus comprises also genetical constructions containing
  • DNA sequences encoding steryl esterases DNA sequences encoding steryl esterases, vectors containing these genetical constructions and hosts expressing the genetical constructions and producing steryl esterases. More specifically, the process of the invention is characterized by what is stated in the characterizing part of claim 1 and 28.
  • the enzyme preparation is, again, characterized by what is stated in the characterizing part of claim 17 and 23.
  • the invention provides considerable advantages.
  • the paper machine runnability is improved due to less breakages of the paper web and decrease in the frequency of cleaning the machine, which also result in better runnability of the paper machine.
  • Due to the increased hydrophilicity of extractives the deresination efficiency is also improved during the pulp washing. The treatment of pulp slurry before bleaching results in better bleachability of the pulp.
  • Figure 1 shows the pH optimum of steryl esterase produced by Rhizomucor miehei
  • Figure 2 shows the pH stability of steryl esterase produced by Rhizomucor miehei
  • Paecilomyces variotii VTT D-76048 assigned as DSM 12587 Trichoderma citrinoviride NTT D-9869, assigned as DSM 12589 Rhizomucor miehei VTT D-82193, assigned as DSM 12588 Futhermore the following strains were deposited according to the Budapest Treaty on March 8, 2000 at the DSMZ- Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1 b, D-38124 Braunschweig, Germany, and assigned as follows:
  • Mechanical pulping stands for manufacturing processes with which the raw material for paper manufacture is defiberized. Mechanical pulp is mainly manufactured by the grinding and refining methods, in which the raw material is subjected to periodical pressure impulses. Due to the friction heat, the structure of the wood is softened and its structure loosened, leading finally to separation of the fibres.
  • TMP thermomechanical pulp
  • GW groundwood pulp
  • PGW pressurized groundwood pulp
  • RMP refiner mechanical pulp
  • PRMP pressurized refiner mechanical pulp
  • CMP chemithermomechamcal pulp.
  • the mechanical pulps are mostly prepared from softwood, but also from aspen wood.
  • “Chemical pulping” stands for manufacturing processes with which substantial amounts of the lignin and hemicellulose components of wood are removed by alkaline cooking methods.
  • “Kraft pulping” is used synonymously with “sulphate cooking” and it designates the cooking method in which sodium sulphide and sodium hydroxide are used as principal cooking chemicals. Mechanical pulping methods save lignin and carbohydrates which results in higher yield compared to chemical pulping.
  • “Sulphite pulp” is made from softwood or from harwood by “sulphite cooking”.
  • “Bleaching” traditionally denoted processes with which the residual lignin of cellulose pulps is solubilized by using chlorine or chlorine dioxide. Presently the pulps are frequently also bleached by oxygen gas, hydrogen peroxide, ozone or by combined sequences including these substances as well as the above-mentioned traditional bleaching chemicals..
  • Process waters are the inner circulation waters of mechanical or chemimechanical pulping process.
  • the process water typically comprises the "brown water” received from the dewatering process of grinded pulp.
  • the process water in this invention thus, e.g. means the water received by processes which enhance the dry matter content of the raw material, such as squeezing or filtering. It is also possible to treat by the methods of this invention the brown water or washing water from mechanical or chemimechanical pulping processes circulated to paper or cardboard machine.
  • the method of the invention is also suitable for the treatment of the process waters of other cellulosic pulps, i.e. chemical pulps.
  • the problems caused by extractives and pitch in paper manufacturing process can be decreased by hydrolyzing enzymatically steryl ester groups in process waters of various pulping processes.
  • process waters are furthermore meant waters which are circulated from the bleaching process or from the paper or board machine totally or partially counterwise to the pulping process or washing waters resulting from the mentioned processes.
  • process waters are meant thus any process waters such as white water or any recycled and reused waters in the pulping processes.
  • various pulps or pulp suspensions can be treated with the process of this invention.
  • the processes of this invention can be applied on pulp suspensions from mechanical, chemimechanical and chemical pulping processes.
  • the enzymatic treatment can be carried out at the head box of the paper machine, where the components of paper or board are mixed together.
  • Paper machine denotes in this context also board and cardboard machines.
  • At least a part of the steryl ester linkages of steryl esters are hydrolyzed resulting in free fatty acids and sterols, which are less prone to form deposits than their esters.
  • This hydrolysis is carried out by using steryl esterases originating from micro-organisms capable of efficiently producing steryl esterase activity into their culture medium.
  • the treatment method of this invention is able to hydrolyze 20 %, preferably 50 %, most preferably 90 % of the steryl esters of the process waters or pulp suspensions. This results in savings of chemical costs and reduction in the frequency of cleaning machines. Furthermore this results in improved washing (deresination efficiency) of the pulp.
  • the amount of hydrolyzed steryl esters is calculated as described in Example 4.
  • the enzyme preparation used comprises the cultivation liquid or medium of a steryl esterase producing microorganism.
  • a cultivation liquid or medium or filtrate or extract is concentrated before use.
  • the enzyme preparation comprises a purified enzyme, isolated from a cultivation liquid or medium.
  • enzyme preparation denotes any product which contains at least one enzyme.
  • an enzyme preparation may be a culture liquor or filtrate containing one or more enzymes, an isolated enzyme or a mixture of one or more enzymes.
  • adjuvants which commonly are used in enzyme preparations intended for application in the pulp and paper industry.
  • adjuvants are typically comprised of, for instance, buffering agents and stabilizing agents.
  • the enzyme preparation useful for treatment of process waters and/or pulp suspensions comprises an essential steryl esterase enzyme activity and may contain also another enzyme activity, preferably lipase activity, lignin degrading activity, hemicellulase or pectinase activity.
  • Suitable microorganism strains which are capable of producing steryl esterases can be isolated from organic matter from a pulp mill or from the vicinity thereof or from any other potential source containing material with this sterol structure.
  • culture collection strains can be screened for their ability to use steryl esters as their carbon source.
  • strains isolated from pulp mill sites, which are capable of degrading steryl esters typically found in the pitch of wood such as sitosteryl ester.
  • the present invention also discloses a method of isolating microbial strains capable of producing steryl esterases. In summary it comprises the steps of:
  • the growth medium contains steryl esters as carbon source together with suitable nitrogen sources. The method is illustrated in more detail in Example 1.
  • Steryl esterases can be produced with a microbial strain or mixed microbial populations isolated according to the above method by cultivating the microorganisms on growth media containing steryl esters.
  • New microorganism strains capable of hydrolyzing the ester linkages in steryl esters can be screened for by using the following method steps:
  • the invention is not, however, limited to the indicated origins of the enzyme or to the isolation method, and the enzyme can also be obtained by other methods.
  • the steryl esterase enzyme by microorganisms, which have been mutated or genetically constructed to produce the desired enzyme, or by other production host strains, to which the gene encoding this enzyme has been transfered.
  • Suitable recombinant DNA methods for constructing steryl esterage producing recombinant strains are known to a person skilled in the art and are described in Sambrook et al (1989) and Glick and Pasternak (1998). Similar methods as are used for constructing recombinant strains producing lipase can be used in constructing strains of this invention and are described for example WO 92/05249 Suitable production hosts for producing recombinant steryl esterases are for example Aspergillus and Trichoderma systems especially for genes originating from fungal sources and Bacillus for genes originating from bacterial sources The screening of steryl esterase producing strains can be done by testing the capability of the strains to hydrolyse cholesterol oleate or some synthetic steryl ester Cholesterol oleate comprises chemically similar linkages as steryl ester comprising substrates
  • the steryl esterase preparation can be derived from a microorganism strain selected from the group consisting of microorganisms of the genera Trichoderma, Paecdomyces,
  • Rhizomucor Candida, Bacillus and Pseudomonas, preferably from Trichoderma, Paecilomyces , Rhizomucor, Candida and Pseudomonas More preferably the strains belong to the species Trichoderma reesei, Trichoderma longibrachiatum, Trichoderma citrinoviride, Paecilomyces variotn, Rhizomucor miehei, Candida tropicahs, Bacillus pum ⁇ us, Bacillus subtihs/macerans, Bacillus subtihs or Pseudomonas stutzeri, most preferably to strains belonging to Paecilomyces variotn, Trichoderma citrinoviride Rhizomucor miehei, Candida tropicahs or Pseudomonas stutzeri, most preferably to strains belonging to Paecilomyces variotn, Trichoderma citrinoviride Rhizomucor
  • the enzyme preparations are prepared by cultivating on a suitable cultivation medium compnsmg steryl esters any of the above-mentioned steryl esterase producing microorganisms
  • Esterases are enzymes, which catalyze the hydrolysis of ester linkages Esterases can be devided according to their substrate specificity to several classes of enzymes Lipases (EC 3 1 1 3) belong to esterases and they hydrolyze ester linkages in t ⁇ glycendes Sterol esterase (EC 3 1 1 13) hydrolyzes steryl esters
  • the present enzyme preparations typically contain suitable adjuvants such as buffering agents or stabilizers, conventionally used in enzyme preparations intended for use on pulp and paper.
  • the steryl esterase treatment can be conducted separately, simultaneously with another enzymatic treatment, or before such a treatment. It is particularly preferred to combine the steryl esterase treatment with a lipase treatment.
  • the steryl esterase treatment is carried out simultaneously with a lipase treatment it is preferred to use an enzyme preparation to which the steryl esterase has been added, or which has been produced by a strain genetically improved to produce high steryl esterase activity, in order to obtain a preparation with an essentially high steryl esterase activity.
  • the steryl esterase treatment can also be combined with treatments with other enzymes like hemicellulases, pectinases and/or lignin modifying (oxidative enzyme) activities.
  • steryl esterase treatment can be carried out with at least one paper making chemical such as alum or fixatives.
  • the process is not limited to a certain wood raw material, but it can be applied generally to both soft and hardwood species, such as species of the order of Pinacae (e.g. the families of Picea and Pinus), Salicaceae (e.g. the family of Populus) and the species in the family of Betula and species of Eucalyptus .
  • soft and hardwood species such as species of the order of Pinacae (e.g. the families of Picea and Pinus), Salicaceae (e.g. the family of Populus) and the species in the family of Betula and species of Eucalyptus .
  • the amount of enzyme needed for hydrolyzing a substantial amount of the steryl esters contained in the process waters or in pulp suspensions is, when calculated as steryl esterase activity, 1 - 1000 nkat/g, preferably 20 - 500 nkat/g pulp or extractives or 1-4000 nkat/g steryl esters, preferably 200 - 1000 nkat g steryl esters. It is possible with these amount of enzymes to hydrolyze 20 %, preferably 50 %, most preferably 90 % of the steryl esters contained in the process waters or in the pulp suspensions of paper manufacturing process.
  • the necessary enzyme activity varies from 1-1000 nkat/ 1, preferably 20 - 500 nkat/1 of process water.
  • Lipase can originate from any microorganism capable of producing this enzyme, such as from the genus Aspergillus or Pseudomonas.
  • Hemicellulose hydrolyzing enzymes can originate from any microorganism capable of producing these enzymes, such as from filamentous fungi Trichoderma, Aspergillus, Penicillium , Paecilomyces, Sclerotium, Sporotrichum, Thielavia, Polyporus, Tyromyces or from bacteria, such as Bacillus or
  • the invention is not, however, limited to the indicated origins of the enzyme nor to the isolation method, and the enzyme can also be obtained by other methods.
  • Lignin modifying enzymes such as laccase can originate from the genus Trametes, for example from Trametes versicolor or Trametes hirsuta or from some other white rot fungi.
  • a commercial lipase preparation such as Resinase ® Novo Nordisk A S) or laccase preparation such as preparation from Trametes villosa (Novo Nordisk -A/S) can be used.
  • the process water and/or pulp suspension is treated by using steryl esterases with or without lipases and possible with other enzymes in 20 - 90 °C, preferable 30-60 °C, 10 min to 24 hours, preferable 0,5- 2 hours.
  • the treatment can be performed in pH 3 to 9, preferably in pH 4 to 8 most preferably 6 to 8. Hence, when treating alkaline pulps the pH adjustment may be needed.
  • the enzyme preparations of this invention should not contain any substantial amount of cellulase activities.
  • Cellulase negative strains not capable of producing one or more cellulases can be prepared by mutation or genetical engineering methods as described for example in US 5,298,405 or alternatively cellulase activity can be removed by well known protein purification methods.
  • Trichoderma citrinoviride VTT D-98697
  • DSM 12589 Trichoderma citrinoviride
  • DCS fraction was prepared from the TMP pulp by diluting it to
  • the reaction mixture contained 1 ml of the culture filtrate and 0.5 mg of cholesterol oleate. The mixture was incubated at 40°C for 18h, whereafter the reaction mixture was evaporated, dissolved in hexane and analyzed by thin layer chromatography.
  • the eluent contains petroleum ether - diethylether - acetic acid (50:50:2) and the visualization of substances was carried out by reaction with sulfuric acid.
  • the hydrolysis of the cholesterol oleate is indicated as +/- in Table 1. Table 1.
  • the most potential strains producing steryl esterase activity were further cultivated at different pH-values using different inducers (DCS water, soy bean meal or cholesterol oleate) in the cultivation media. After the cultivation (+30 °C, 7 d) the culture filtrate was separated by centrifugation, whereafter the sterol esterase and lipase activities were measured.
  • DCS water, soy bean meal or cholesterol oleate inducers
  • the sterol esterase activity was measured by spectrophotometer.
  • the reaction mixture contained 3 ml of 0,7 M phosphate buffer (pH 7,5), 100 ⁇ l of cholesterol oleate solution, 20 ⁇ l of H 2 O 2 solution, 20 ⁇ l of catalase solution, 20 ⁇ l of cholesterol oxidase and 50 ⁇ l of sample solution (culture filtrate).
  • the increase of absorbance at 240 nm was a measurement of the cholesterol esterase activity.
  • One unit of esterase activity (nkat) was destined as the amount of enzyme that liberated 1 nmol cholesterol.
  • soya bean meal was the most efficient inducor.
  • Treating TMP process waters with an enzyme preparation comprising steryl esterase activity Treating TMP process waters with an enzyme preparation comprising steryl esterase activity.
  • DCS fraction was isolated from spruce TMP.
  • DCS fraction containing extractives was treated with sterol esterase (lOx concentrated culture filtrate) produced in Example 3.
  • the analysis of the extractives was carried out by gas chromatography after extraction with ether (Orsa and Holmbom, 1994) The results are shown in Table 3.
  • the steryl ester content was 45.6 mg/1 .
  • the steryl ester content was reduced even by 52 and 38 %.
  • Table 3 Hydrolysis of sterol esters and triglycerides present in DCS- fraction of TMP pulp by different fungi
  • the characteristics of the sterol esterase produced by Rhizomucor miehei were determined.
  • the enzyme was found to have pH optimum in the range of 6-8 (see Figure 1).
  • the sterol esterase was stable at pH-values of 4-8 (see Figure 2) .
  • a soy bean meal medium contained:
  • pH was adjusted to 4.5 - 5.5 for fungi and to pH 6,5 for bacteria.
  • Distiller's spent grain -Solca floe- soy bean meal medium contained:
  • the steryl esterases of R. miehei was partially purified with hydrophobic interaction chromatography (HIC).
  • HIC hydrophobic interaction chromatography
  • 1% DCS fraction was treated with dosage 4000 nkat of esterase /g of steryl esters (40°C, pH 5, 20 h) after which the samples were analysed by GC (Table 6).
  • Purified R. miehei esterase hydrolysed over 50 % of steryl esters, but only 70 % of triglycerides were hydrolysed, which indicates that it is advantageous to combine steryl esterase treatment with lipase treatment.
  • Table 6 Treating of DCS waters with R miehei esterase.
  • T. reesei QM 6a ATCC 13631
  • T. reesei Rut C- 30 ATCC 56765
  • T. reesei QM 9136 ATCC 26920 is a cellulase negative mutant strain, which produces no or only a hardly measurable amount of cellulase measured as HEC activity.
  • Ad d ress ol depositary institution iinctuain postal coae ana coumrvi

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Abstract

L'invention concerne un procédé et une préparation à base d'enzymes pour le contrôle enzymatique d'agents d'extraction pendant la fabrication du papier. Les suspensions pâteuses et/ou les eaux de traitement provenant de réduction en pâte, le blanchiment et/ou le procédé de fabrication de papier sont traités à l'aide d'une préparation à base d'enzymes qui consiste en une activité enzymatique capable d'hydrolyser la liaison ester dans les stérylesters d'agents d'extraction du bois. Du traitement enzymatique, il en résulte une amélioration de l'aptitude au passage de la machine à papier et moins de taches collantes et de jaunissement dans le papier et, par conséquent, une meilleure qualité de l'article en papier.
PCT/FI2000/000180 1999-03-08 2000-03-08 Procede et preparation enzymatique a activite de sterylesterase permettant de controler la poix pendant la fabrication de papier WO2000053843A1 (fr)

Priority Applications (1)

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AU32936/00A AU3293600A (en) 1999-03-08 2000-03-08 A process and an enzyme preparation with steryl esterase activity for controlling pitch during paper manufacture

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FI990501 1999-03-08
FI990501A FI990501L (fi) 1999-03-08 1999-03-08 Uusi entsymaattinen prosessi paperinvalmistuksen pihkaongelmien kontro lloimiseksi

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002075045A1 (fr) * 2001-03-16 2002-09-26 Consejo Superior De Investigaciones Científicas Procede d'elimination enzymatique des depots de brai (poix) formes durant la fabrication de la pate a papier a l'aide d'une esterase hydrolysant les triglycerides et les esters d'esterols
WO2003035972A1 (fr) * 2001-10-23 2003-05-01 Novozymes A/S Enzymes d'oxydation utilisees dans la fabrication de materiaux papiers
WO2007095575A1 (fr) * 2006-02-14 2007-08-23 Novozymes North America, Inc Procedes et compositions de traitement de pate chimique
CN102154867A (zh) * 2010-12-29 2011-08-17 维达纸业(孝感)有限公司 一种生活用纸浆料的预处理方法
WO2012149192A1 (fr) 2011-04-28 2012-11-01 Novozymes, Inc. Polypeptides à activité endoglucanase et polynucléotides codant pour les polypeptides
EP3722418A1 (fr) * 2019-04-08 2020-10-14 AB Enzymes Oy Composition d'enzyme stable en solution
WO2024055376A1 (fr) * 2022-09-15 2024-03-21 华南理工大学 Procédé permettant de traiter un adhésif dans de la pâte recyclée au moyen de pectine lyase et utilisation

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WO2002075045A1 (fr) * 2001-03-16 2002-09-26 Consejo Superior De Investigaciones Científicas Procede d'elimination enzymatique des depots de brai (poix) formes durant la fabrication de la pate a papier a l'aide d'une esterase hydrolysant les triglycerides et les esters d'esterols
ES2179768A1 (es) * 2001-03-16 2003-01-16 Consejo Superior Investigacion Procedimiento para el control enzimatico de los depositos de brea (pitch) formados durante la fabricacion de pasta de papel utilizando una esterasa que hidroliza tanto trigliceridos como esteres de esteroles.
ES2179768B1 (es) * 2001-03-16 2004-04-16 Consejo Superior De Investigaciones Cientificas Esterasa, procedimiento de obtencion y su utilizacion para el control enzimatico de los depositos de brea (pitch) formados durante la fabricacion de pasta de papel.
WO2003035972A1 (fr) * 2001-10-23 2003-05-01 Novozymes A/S Enzymes d'oxydation utilisees dans la fabrication de materiaux papiers
CN100336970C (zh) * 2001-10-23 2007-09-12 诺维信公司 纸材料制造中的氧化酶
WO2007095575A1 (fr) * 2006-02-14 2007-08-23 Novozymes North America, Inc Procedes et compositions de traitement de pate chimique
CN102154867A (zh) * 2010-12-29 2011-08-17 维达纸业(孝感)有限公司 一种生活用纸浆料的预处理方法
CN102154867B (zh) * 2010-12-29 2012-12-05 维达纸业(孝感)有限公司 一种生活用纸浆料的预处理方法
WO2012149192A1 (fr) 2011-04-28 2012-11-01 Novozymes, Inc. Polypeptides à activité endoglucanase et polynucléotides codant pour les polypeptides
EP3722418A1 (fr) * 2019-04-08 2020-10-14 AB Enzymes Oy Composition d'enzyme stable en solution
WO2020208296A1 (fr) * 2019-04-08 2020-10-15 Ab Enzymes Oy Composition enzymatique stable en solution
CN113631705A (zh) * 2019-04-08 2021-11-09 生化酶股份有限公司 溶液稳定的酶组合物
JP2022529140A (ja) * 2019-04-08 2022-06-17 アーベー エンジュメス オサケ ユキチュア 溶液安定性の酵素組成物
WO2024055376A1 (fr) * 2022-09-15 2024-03-21 华南理工大学 Procédé permettant de traiter un adhésif dans de la pâte recyclée au moyen de pectine lyase et utilisation

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