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WO2000065098A3 - Nucleotide extension on a microarray of gel-immobilized primers - Google Patents

Nucleotide extension on a microarray of gel-immobilized primers Download PDF

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Publication number
WO2000065098A3
WO2000065098A3 PCT/US2000/011286 US0011286W WO0065098A3 WO 2000065098 A3 WO2000065098 A3 WO 2000065098A3 US 0011286 W US0011286 W US 0011286W WO 0065098 A3 WO0065098 A3 WO 0065098A3
Authority
WO
WIPO (PCT)
Prior art keywords
primers
gel
microarray
nucleotide extension
immobilized primers
Prior art date
Application number
PCT/US2000/011286
Other languages
French (fr)
Other versions
WO2000065098A9 (en
WO2000065098A2 (en
Inventor
Svetlana Dubiley
Eugene Kirillov
Andrei Mirzabekov
Original Assignee
Univ Chicago
Svetlana Dubiley
Eugene Kirillov
Andrei Mirzabekov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Chicago, Svetlana Dubiley, Eugene Kirillov, Andrei Mirzabekov filed Critical Univ Chicago
Priority to AU46691/00A priority Critical patent/AU4669100A/en
Priority to EP00928451A priority patent/EP1171637A2/en
Priority to CA002369524A priority patent/CA2369524A1/en
Publication of WO2000065098A2 publication Critical patent/WO2000065098A2/en
Publication of WO2000065098A3 publication Critical patent/WO2000065098A3/en
Publication of WO2000065098A9 publication Critical patent/WO2000065098A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and compositions have been developed for nucleotide extension of primers immobilized within gel pads on a microchip using multibase primers or multiple sets of primers, or combinations thereof. Molecules or parts of molecules are identified. The single base extension was amplified by carrying out the reaction under elevated temperature.
PCT/US2000/011286 1999-04-27 2000-04-25 Nucleotide extension on a microarray of gel-immobilized primers WO2000065098A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU46691/00A AU4669100A (en) 1999-04-27 2000-04-25 Nucleotide extension on a microarray of gel-immobilized primers
EP00928451A EP1171637A2 (en) 1999-04-27 2000-04-25 Nucleotide extension on a microarray of gel-immobilized primers
CA002369524A CA2369524A1 (en) 1999-04-27 2000-04-25 Nucleotide extension on a microarray of gel-immobilized primers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30067599A 1999-04-27 1999-04-27
US09/300,675 1999-04-27

Publications (3)

Publication Number Publication Date
WO2000065098A2 WO2000065098A2 (en) 2000-11-02
WO2000065098A3 true WO2000065098A3 (en) 2001-07-19
WO2000065098A9 WO2000065098A9 (en) 2001-08-09

Family

ID=23160129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/011286 WO2000065098A2 (en) 1999-04-27 2000-04-25 Nucleotide extension on a microarray of gel-immobilized primers

Country Status (4)

Country Link
EP (1) EP1171637A2 (en)
AU (1) AU4669100A (en)
CA (1) CA2369524A1 (en)
WO (1) WO2000065098A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7179638B2 (en) 1999-07-30 2007-02-20 Large Scale Biology Corporation Microarrays and their manufacture by slicing
US6713309B1 (en) 1999-07-30 2004-03-30 Large Scale Proteomics Corporation Microarrays and their manufacture
WO2001085988A1 (en) * 2000-05-09 2001-11-15 Diatech Pty. Ltd. Methods for detecting nucleic acid molecules having particular nucleotide sequences
US20030073091A1 (en) * 2000-12-28 2003-04-17 The University Of Chicago Use of generic oligonucleotide microchips to detect protein-nucleic acid interactions
WO2003033691A1 (en) * 2001-10-19 2003-04-24 Institut Molekulyarnoi Biologii Im. V.A. Engelgardta Rossiiskoi Akademii Nauk Cellular microchip and the use thereof for method for examining living cells
US7049073B2 (en) 2002-10-30 2006-05-23 The University Of Chicago Double stranded nucleic acid biochips
CN105969848A (en) * 2012-03-29 2016-09-28 三菱丽阳株式会社 Microarray for detection of mutations in beta-globin genes
EP3577233B1 (en) 2017-03-31 2021-07-14 Marcella Chiari Genotyping of mutations by combination of in-tube hybridization and universal tag-microarray

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
WO1992010587A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microfluorescence detection
EP0535242A1 (en) * 1991-03-18 1993-04-07 Institut Molekulyarnoi Biologii Imeni V.A. Engelgardta Akademii Nauk Sssr Method and device for determining nucleotide sequence of dna
WO1993025563A1 (en) * 1992-06-17 1993-12-23 City Of Hope A method of detecting and discriminating between nucleic acid sequences
EP0698792A1 (en) * 1993-05-10 1996-02-28 Nissui Pharmaceutical Co., Ltd. Method for assaying more than one immunological ligand, and assay reagent and kit therefor
WO1996031622A1 (en) * 1995-04-07 1996-10-10 Oxford Gene Technology Limited Detecting dna sequence variations
US5770721A (en) * 1993-08-11 1998-06-23 University Of Chicago Method of manufacturing a matrix for the detection of mismatches
WO1998028438A1 (en) * 1996-12-20 1998-07-02 Diatech Pty. Ltd. Process for detecting point mutations in which the primer is bound to a solid support and extension does not occur if the terminal nucleotide(s) mis-match
WO1998044152A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid sequencing
EP0905517A1 (en) * 1997-03-10 1999-03-31 Nissui Pharmaceutical Co., Ltd. Analytical method, kit, and apparatus

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
WO1992010587A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microfluorescence detection
EP0535242A1 (en) * 1991-03-18 1993-04-07 Institut Molekulyarnoi Biologii Imeni V.A. Engelgardta Akademii Nauk Sssr Method and device for determining nucleotide sequence of dna
WO1993025563A1 (en) * 1992-06-17 1993-12-23 City Of Hope A method of detecting and discriminating between nucleic acid sequences
EP0698792A1 (en) * 1993-05-10 1996-02-28 Nissui Pharmaceutical Co., Ltd. Method for assaying more than one immunological ligand, and assay reagent and kit therefor
US5770721A (en) * 1993-08-11 1998-06-23 University Of Chicago Method of manufacturing a matrix for the detection of mismatches
WO1996031622A1 (en) * 1995-04-07 1996-10-10 Oxford Gene Technology Limited Detecting dna sequence variations
WO1998028438A1 (en) * 1996-12-20 1998-07-02 Diatech Pty. Ltd. Process for detecting point mutations in which the primer is bound to a solid support and extension does not occur if the terminal nucleotide(s) mis-match
EP0905517A1 (en) * 1997-03-10 1999-03-31 Nissui Pharmaceutical Co., Ltd. Analytical method, kit, and apparatus
WO1998044152A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid sequencing

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DUBILEY ET AL.: "POLYMORPHISM ANALYSIS AND GENE DETECTION BY MINISEQUENCING ON AN ARRAY OF GEL-IMMOBILIZED PRIMERS", NUCLEIC ACIDS RESEARCH/ONLINE, vol. 27, no. 18, August 1999 (1999-08-01), pages e19, XP002153648 *
MCKENZIE STEVEN E ET AL: "Parallel molecular genetic analysis.", EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 6, no. 5, 1998, pages 417 - 429, XP000953351, ISSN: 1018-4813 *
SYVANEN ANN-CHRISTINE: "From gels to chips: "minisequencing" primer extension for analysis of point mutations and single nucleotide polymorphisms.", HUMAN MUTATION, vol. 13, no. 1, 6 April 1999 (1999-04-06), pages 1 - 10, XP000953256, ISSN: 1059-7794 *
YERSHOV G ET AL: "DNA ANALYSIS AND DIAGNOSTICS ON OLIGONUCLEOTIDE MICROCHIPS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, 1 May 1996 (1996-05-01), pages 4913 - 4918, XP002064987, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU4669100A (en) 2000-11-10
WO2000065098A9 (en) 2001-08-09
WO2000065098A2 (en) 2000-11-02
CA2369524A1 (en) 2000-11-02
EP1171637A2 (en) 2002-01-16

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