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WO2000071579A3 - Regulation de l'organite de l'homeostasie dans une production de cellule - Google Patents

Regulation de l'organite de l'homeostasie dans une production de cellule Download PDF

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Publication number
WO2000071579A3
WO2000071579A3 PCT/EP2000/004718 EP0004718W WO0071579A3 WO 2000071579 A3 WO2000071579 A3 WO 2000071579A3 EP 0004718 W EP0004718 W EP 0004718W WO 0071579 A3 WO0071579 A3 WO 0071579A3
Authority
WO
WIPO (PCT)
Prior art keywords
organelle
homeostasis
gene
regulating
cell factory
Prior art date
Application number
PCT/EP2000/004718
Other languages
English (en)
Other versions
WO2000071579A2 (fr
Inventor
Roelof Ary Lans Bovenberg
Den Berg Marco Alexander Van
Maarten Nieboer
Marten Veenhuis
Jan Andries Kornelis Will Kiel
Der Klei Ida Johanna Van
Original Assignee
Dsm Nv
Roelof Ary Lans Bovenberg
Den Berg Marco Alexander Van
Maarten Nieboer
Marten Veenhuis
Jan Andries Kornelis Will Kiel
Klei Ida Johanna V D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv, Roelof Ary Lans Bovenberg, Den Berg Marco Alexander Van, Maarten Nieboer, Marten Veenhuis, Jan Andries Kornelis Will Kiel, Klei Ida Johanna V D filed Critical Dsm Nv
Priority to EP00931260A priority Critical patent/EP1179071A2/fr
Priority to AU49249/00A priority patent/AU4924900A/en
Publication of WO2000071579A2 publication Critical patent/WO2000071579A2/fr
Publication of WO2000071579A3 publication Critical patent/WO2000071579A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/385—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Penicillium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/78—Hansenula
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/80—Penicillium
    • C12R2001/82—Penicillium chrysogenum

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode permettant de moduler la production de métabolites secondaires dans le champignon Penicillium chrysogenum par influence des organites de l'homéostasie par modulation d'au moins un gène directement lié à l'organite de l'homéostasie dans ce champignon. De préférence, l'organite de l'homéostasie est modulé par organisation génétique d'au moins un gène codant pour cette protéine responsable de la biogenèse de l'organite (protéine dont l'expression et/ou l'activité doit être augmentée) et/ou au moins un gène codant pour une protéine responsable de la dégénérescence de l'organite (protéine dont l'expression et/ou l'activité doit être réduite). Des gènes intervenant dans la biosynthèse de l'organite dans le P. chrysogenum sont illustrés par le gène dénommé pex11. Des gènes intervenant dans la dégradation de l'organite sont illustrés par les gènes dénommés vps15 et pdd1.
PCT/EP2000/004718 1999-05-21 2000-05-22 Regulation de l'organite de l'homeostasie dans une production de cellule WO2000071579A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00931260A EP1179071A2 (fr) 1999-05-21 2000-05-22 Regulation de l'organite de l'homeostasie dans une production de cellule
AU49249/00A AU4924900A (en) 1999-05-21 2000-05-22 Regulating organelle homeostasis in a cell factory

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99201630 1999-05-21
EP99201630.3 1999-05-21

Publications (2)

Publication Number Publication Date
WO2000071579A2 WO2000071579A2 (fr) 2000-11-30
WO2000071579A3 true WO2000071579A3 (fr) 2001-03-01

Family

ID=8240226

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004718 WO2000071579A2 (fr) 1999-05-21 2000-05-22 Regulation de l'organite de l'homeostasie dans une production de cellule

Country Status (3)

Country Link
EP (1) EP1179071A2 (fr)
AU (1) AU4924900A (fr)
WO (1) WO2000071579A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014003555A1 (fr) * 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Production améliorée de pénicilline

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0448180A2 (fr) * 1990-03-23 1991-09-25 Gist-Brocades N.V. Méthode pour moduler la production des métabolites secondaires
WO1994024289A1 (fr) * 1993-04-19 1994-10-27 Eurolysine Procede d'adressage de proteines dans les peroxysomes de levures
WO1997038107A1 (fr) * 1996-04-04 1997-10-16 Gist-Brocades B.V. Enzyme possedant une forte activite d'adipoyl-coenzyme a synthetase et utilisations de celle-ci

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0448180A2 (fr) * 1990-03-23 1991-09-25 Gist-Brocades N.V. Méthode pour moduler la production des métabolites secondaires
WO1994024289A1 (fr) * 1993-04-19 1994-10-27 Eurolysine Procede d'adressage de proteines dans les peroxysomes de levures
WO1997038107A1 (fr) * 1996-04-04 1997-10-16 Gist-Brocades B.V. Enzyme possedant une forte activite d'adipoyl-coenzyme a synthetase et utilisations de celle-ci

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARSHALL P ET AL: "Pmp27 promotes peroxisomal proliferation", J. CELL BIOL., vol. 129, no. 2, April 1995 (1995-04-01), pages 345 - 355, XP002120786 *
ROGGENKAMP R ET AL: "Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha", MOL CELL BIOL., vol. 9, no. 3, March 1989 (1989-03-01), pages 988 - 994, XP000037387 *
SAKAI Y ET AL: "The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae", J BACTERIOL., vol. 177, December 1995 (1995-12-01), pages 6773 - 6781, XP002120787 *

Also Published As

Publication number Publication date
WO2000071579A2 (fr) 2000-11-30
AU4924900A (en) 2000-12-12
EP1179071A2 (fr) 2002-02-13

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