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WO2000008138A3 - Ribosomal rna (rrn) operon altered bacteria and uses thereof - Google Patents

Ribosomal rna (rrn) operon altered bacteria and uses thereof Download PDF

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Publication number
WO2000008138A3
WO2000008138A3 PCT/US1999/017692 US9917692W WO0008138A3 WO 2000008138 A3 WO2000008138 A3 WO 2000008138A3 US 9917692 W US9917692 W US 9917692W WO 0008138 A3 WO0008138 A3 WO 0008138A3
Authority
WO
WIPO (PCT)
Prior art keywords
operon
rrn
ribosomal rna
altered bacteria
relates
Prior art date
Application number
PCT/US1999/017692
Other languages
French (fr)
Other versions
WO2000008138A2 (en
Inventor
Catherine L Squires
Tsuneaki Asai
Original Assignee
Tufts College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tufts College filed Critical Tufts College
Priority to AU53369/99A priority Critical patent/AU5336999A/en
Publication of WO2000008138A2 publication Critical patent/WO2000008138A2/en
Publication of WO2000008138A3 publication Critical patent/WO2000008138A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a genetically manipulated organism useful in the screening of novel proliferation modulating compounds. More specifically, this invention relates to an rRNA operon alterable bacterium useful for the selection of antibiotics against pathogenic microorganisms.
PCT/US1999/017692 1998-08-05 1999-08-04 Ribosomal rna (rrn) operon altered bacteria and uses thereof WO2000008138A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU53369/99A AU5336999A (en) 1998-08-05 1999-08-04 Novel rrn operon altered bacteria and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9582298P 1998-08-05 1998-08-05
US60/095,822 1998-08-05

Publications (2)

Publication Number Publication Date
WO2000008138A2 WO2000008138A2 (en) 2000-02-17
WO2000008138A3 true WO2000008138A3 (en) 2001-02-01

Family

ID=22253740

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/017692 WO2000008138A2 (en) 1998-08-05 1999-08-04 Ribosomal rna (rrn) operon altered bacteria and uses thereof

Country Status (2)

Country Link
AU (1) AU5336999A (en)
WO (1) WO2000008138A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2004825B1 (en) * 2006-03-31 2010-03-10 Eidgenoessische Technische Hochschule Zürich ETH Transfer Screening assay for ribosomal antibiotics
IL307456A (en) * 2013-03-15 2023-12-01 Cibus Us Llc Methods and compositions for increasing efficiency of increased efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US10400291B2 (en) 2014-06-27 2019-09-03 The Regents Of The University Of Michigan Method for bacterial species identification and strain typing

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ASAI TSUNEAKI ET AL: "An Escherichia coli strain with all chromosomal rRNA operons inactivated: Complete exchange of rRNA genes between bacteria.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 5, 2 March 1999 (1999-03-02), March 2, 1999, pages 1971 - 1976, XP002145403, ISSN: 0027-8424 *
ASAI TSUNEAKI ET AL: "Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.", JOURNAL OF BACTERIOLOGY, vol. 181, no. 12, June 1999 (1999-06-01), pages 3803 - 3809, XP002145402, ISSN: 0021-9193 *
CONDON CIARAN ET AL: "RRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation.", JOURNAL OF BACTERIOLOGY, vol. 177, no. 14, 1995, pages 4152 - 4156, XP002145399, ISSN: 0021-9193 *
OKUSU HARUKO ET AL: "AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (MAR) mutants.", JOURNAL OF BACTERIOLOGY, vol. 178, no. 1, 1996, pages 306 - 308, XP002145401, ISSN: 0021-9193 *
PRILIPOV ALEXEJ ET AL: "Coupling site-directed mutagenesis with high-level expression: Large scale production of mutant porins from E. coli.", FEMS MICROBIOLOGY LETTERS, vol. 163, no. 1, 1 June 1998 (1998-06-01), pages 65 - 72, XP000917840, ISSN: 0378-1097 *
SIGMUND C D ET AL: "ERYTHROMYCIN RESISTANCE DUE TO A MUTATION IN A RIBOSOMAL RNA OPERON OF ESCHERICHIA-COLI", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 79, no. 18, 1982, 1982, pages 5602 - 5606, XP000917839, ISSN: 0027-8424 *
STEVENSON BRADLEY S ET AL: "Growth rate-dependent accumulation of RNA from plasmid-borne rRNA operons in Escherichia coli.", JOURNAL OF BACTERIOLOGY, vol. 180, no. 7, April 1998 (1998-04-01), pages 1970 - 1972, XP002145400, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
AU5336999A (en) 2000-02-28
WO2000008138A2 (en) 2000-02-17

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