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WO2001049837A3 - Procede de synthese de l'adn telomerique de vertebres, produit obtenu selon ce procede et utilisations correspondantes - Google Patents

Procede de synthese de l'adn telomerique de vertebres, produit obtenu selon ce procede et utilisations correspondantes Download PDF

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Publication number
WO2001049837A3
WO2001049837A3 PCT/EP2001/000043 EP0100043W WO0149837A3 WO 2001049837 A3 WO2001049837 A3 WO 2001049837A3 EP 0100043 W EP0100043 W EP 0100043W WO 0149837 A3 WO0149837 A3 WO 0149837A3
Authority
WO
WIPO (PCT)
Prior art keywords
telomers
yeasts
telomeric dna
length
protein
Prior art date
Application number
PCT/EP2001/000043
Other languages
English (en)
Other versions
WO2001049837A2 (fr
Inventor
Pierluigi Donini
Fiorentina Ascenzioni
Eric Gilson
Original Assignee
Univ Roma
Pierluigi Donini
Fiorentina Ascenzioni
Eric Gilson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Roma, Pierluigi Donini, Fiorentina Ascenzioni, Eric Gilson filed Critical Univ Roma
Priority to AU35393/01A priority Critical patent/AU3539301A/en
Publication of WO2001049837A2 publication Critical patent/WO2001049837A2/fr
Publication of WO2001049837A3 publication Critical patent/WO2001049837A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne un procédé qui permet d'obtenir un ADN télomérique humain en introduisant dans une levure de télomérase-h une ou plusieurs séquences géniques d'au moins une protéine télomérique humaine telle que la TFF1p. La présence de cette protéine au moins dans des levures qui synthétisent les télomères de vertébrés permet de réguler la longueur des télomères et la propagation exacte des chromosomes artificiels de mammifères (MAC) et, par conséquent, de respecter la longueur des télomères. Les levures de l'invention sont modifiées en ce qu'elles contiennent un plasmide dans lequel est inséré au moins un gène d'au moins une protéine télomérique humaine telle que la protéine précitée, ce qui permet à ces levures de produire un ADN télomérique avec des télomères d'au moins 250 pb de longueur. En particulier, la cellule est Saccharomyces Cerevisiae DBVPG 4P, déposée le 27.10.99.
PCT/EP2001/000043 2000-01-05 2001-01-04 Procede de synthese de l'adn telomerique de vertebres, produit obtenu selon ce procede et utilisations correspondantes WO2001049837A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU35393/01A AU3539301A (en) 2000-01-05 2001-01-04 Process for the synthesis of vertebrate telomeric dna, products thus obtained and corresponding uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2000A000004 2000-01-05
IT2000RM000004A IT1316981B1 (it) 2000-01-05 2000-01-05 Processo di sintesi di dna telomerico di vertebrato, prodotti cosiottenuti e relativi impieghi.

Publications (2)

Publication Number Publication Date
WO2001049837A2 WO2001049837A2 (fr) 2001-07-12
WO2001049837A3 true WO2001049837A3 (fr) 2002-01-17

Family

ID=11454316

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/000043 WO2001049837A2 (fr) 2000-01-05 2001-01-04 Procede de synthese de l'adn telomerique de vertebres, produit obtenu selon ce procede et utilisations correspondantes

Country Status (3)

Country Link
AU (1) AU3539301A (fr)
IT (1) IT1316981B1 (fr)
WO (1) WO2001049837A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008964A1 (fr) * 1996-08-26 1998-03-05 Tsuneko Okazaki Chromosomes artificiels de mammifere
WO1999015662A1 (fr) * 1997-09-26 1999-04-01 The Rockefeller University Facteurs de fixation des regions de repetition d'une proteine telomere et utilisation diagnostique et therapeutique de ces facteurs
EP0959134A1 (fr) * 1996-09-18 1999-11-24 Dnavec Research Inc. Chromosome artificiel

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008964A1 (fr) * 1996-08-26 1998-03-05 Tsuneko Okazaki Chromosomes artificiels de mammifere
EP0959134A1 (fr) * 1996-09-18 1999-11-24 Dnavec Research Inc. Chromosome artificiel
WO1999015662A1 (fr) * 1997-09-26 1999-04-01 The Rockefeller University Facteurs de fixation des regions de repetition d'une proteine telomere et utilisation diagnostique et therapeutique de ces facteurs

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BACHAND F. ET AL.: "Functional reconstitution of human telomerase expressed in Saccharomyces cerevisiae", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 274, no. 53, 31 December 1999 (1999-12-31), BALTIMORE, MD., US, pages 38027 - 38031, XP002172463, ISSN: 0021-9258 *
BIANCHI ALESSANDRO ET AL: "TFR1 is a dimer and bends telomeric DNA.", EMBO JOURNAL, vol. 16, no. 7, 1997, HEIDELBERG DE, pages 1785 - 1794, XP002172464, ISSN: 0261-4189 *
GARI ELOI ET AL: "A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae.", YEAST, vol. 13, no. 9, 1997, pages 837 - 848, XP000926574, ISSN: 0749-503X *
HENNING KARLA A ET AL: "Humanizing the yeast telomerase template.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 95, no. 10, 12 May 1998 (1998-05-12), May 12, 1998, pages 5667 - 5671, XP002172462, ISSN: 0027-8424 *
HUXLEY C: "Mammalian artificial chromosomes and chromosome transgenics", TRENDS IN GENETICS, AMSTERDAM, NL, vol. 13, no. 9, 1 September 1997 (1997-09-01), pages 345 - 347, XP004093527, ISSN: 0168-9525 *
TAYLOR S S ET AL: "ADDITION OF FUNCTIONAL HUMAN TELOMERES TO YACS", HUMAN MOLECULAR GENETICS, SURREY, GB, vol. 3, no. 8, 1 August 1994 (1994-08-01), pages 1383 - 1386, XP000601479, ISSN: 0964-6906 *

Also Published As

Publication number Publication date
AU3539301A (en) 2001-07-16
WO2001049837A2 (fr) 2001-07-12
IT1316981B1 (it) 2003-05-26
ITRM20000004A0 (it) 2000-01-05
ITRM20000004A1 (it) 2001-07-05

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