WO2001060840A3 - Estimation des proteines/de la solubilite et du repliage par complementation structurelle - Google Patents
Estimation des proteines/de la solubilite et du repliage par complementation structurelle Download PDFInfo
- Publication number
- WO2001060840A3 WO2001060840A3 PCT/US2001/005097 US0105097W WO0160840A3 WO 2001060840 A3 WO2001060840 A3 WO 2001060840A3 US 0105097 W US0105097 W US 0105097W WO 0160840 A3 WO0160840 A3 WO 0160840A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein
- folding
- improper
- marker protein
- protein solubility
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000003556 assay Methods 0.000 title 1
- 239000003550 marker Substances 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000001594 aberrant effect Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 102000035118 modified proteins Human genes 0.000 abstract 1
- 108091005573 modified proteins Proteins 0.000 abstract 1
- 230000012846 protein folding Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01909281A EP1257829B1 (fr) | 2000-02-14 | 2001-02-14 | Estimation de la solubilite et du repliage des proteines par complementation structurelle |
JP2001560224A JP4772247B2 (ja) | 2000-02-14 | 2001-02-14 | タンパク質の溶解性及びフォールディングを評価する相補性アッセイ |
DK01909281T DK1257829T3 (da) | 2000-02-14 | 2001-02-14 | Komplementationsassay der vurderer proteinoplöselighed og -foldning |
CA2398834A CA2398834C (fr) | 2000-02-14 | 2001-02-14 | Repliement proteique/de solubilite evalue par complementation structurale |
DE60123755T DE60123755T2 (de) | 2000-02-14 | 2001-02-14 | Komplementierungsassay für den nachweis von protein-löslichkeit und -faltung |
AU3705501A AU3705501A (en) | 2000-02-14 | 2001-02-14 | Protein/solubility folding assessed by structural complementation |
AU2001237055A AU2001237055B2 (en) | 2000-02-14 | 2001-02-14 | Complementation assay assessing protein solubility and folding |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18228300P | 2000-02-14 | 2000-02-14 | |
US60/182,283 | 2000-02-14 | ||
US77505100A | 2001-01-31 | 2001-01-31 | |
US09/775,051 | 2001-01-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001060840A2 WO2001060840A2 (fr) | 2001-08-23 |
WO2001060840A3 true WO2001060840A3 (fr) | 2002-04-25 |
Family
ID=22667801
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/005097 WO2001060840A2 (fr) | 2000-02-14 | 2001-02-14 | Estimation des proteines/de la solubilite et du repliage par complementation structurelle |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2001060840A2 (fr) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9511074B2 (en) | 2009-08-03 | 2016-12-06 | University of Pittsburgh—of the Commonwealth System of Higher Education | Methods of treating disorders associated with protein polymerization |
US9844605B2 (en) * | 2009-11-05 | 2017-12-19 | The University of Pittsburgh—Of the Commonwealth System of Higher Education | Transgenic Caenorhabditis elegans comprising a human protein with a tendency to aggregate fused to a fluorescent protein |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2883886B1 (fr) * | 2005-04-05 | 2010-12-03 | Biomethodes | Methode d'obtention de variants solubles ou mieux exprimes d'une proteine d'interet |
CN101443460B (zh) | 2006-03-13 | 2013-03-06 | 利兰·斯坦福青年大学托管委员会 | 利用降低亲和力酶互补报告分子系统检测分子间相互作用 |
US8101373B2 (en) | 2007-10-12 | 2012-01-24 | Discoverx Corporation | β-galactosidase donor fragments |
US9072772B2 (en) | 2009-11-05 | 2015-07-07 | University of Pittsburgh—of the Commonwealth System of Higher Education | Methods of treating disorders associated with protein aggregation |
CA2840269C (fr) | 2011-06-23 | 2015-08-18 | Discoverx Corporation | Suivi du trafic des proteines par complementation d'un fragment rapporteur de beta-galactosidase |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998034120A1 (fr) * | 1997-01-31 | 1998-08-06 | Universite De Montreal | Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires |
WO1998044350A1 (fr) * | 1997-04-02 | 1998-10-08 | The Board Of Trustees Of The Leland Stanford Junior University | Detection d'interactions moleculaires par complementation de sous-unites de marquage |
-
2001
- 2001-02-14 WO PCT/US2001/005097 patent/WO2001060840A2/fr active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998034120A1 (fr) * | 1997-01-31 | 1998-08-06 | Universite De Montreal | Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires |
WO1998044350A1 (fr) * | 1997-04-02 | 1998-10-08 | The Board Of Trustees Of The Leland Stanford Junior University | Detection d'interactions moleculaires par complementation de sous-unites de marquage |
Non-Patent Citations (6)
Title |
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ABBAS-TERKI TOUFIK ET AL: "alpha-complemented beta-galactosidase. An in vivo model substrate for the molecular chaperone heat-shock protein 90 in yeast.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 266, no. 2, December 1999 (1999-12-01), pages 517 - 523, XP002182770, ISSN: 0014-2956 * |
CAMPBELL VALOIS F -X ET AL: "Utilisation of the PCA strategy to study the folding of the RBD of raf.", FASEB JOURNAL, vol. 13, no. 7, 23 April 1999 (1999-04-23), Annual Meeting of the American Societies for Experimental Biology on Biochemistry and Molecular Biology 99;San Francisco, California, USA; May 16-20, 1999, pages A1387, XP002182768, ISSN: 0892-6638 * |
JOHNSSON N ET AL: "SPLIT UBIQUITIN AS A SENSOR OF PROTEIN INTERACTIONS INV VIVO", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 October 1994 (1994-10-01), pages 10340 - 10344, XP002064564, ISSN: 0027-8424 * |
KO Y H ET AL: "THE CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR. OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF WILD TYPE ANDDELTAF508 MUTANT FORMS OF THE FIRST NUCLEOTIDE BINDING FOLD IN FUSION WITH THE MALTOSE-BINDING PROTEIN", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 268, no. 32, 15 November 1993 (1993-11-15), pages 24330 - 24338, XP002055212, ISSN: 0092-8674 * |
NIXON ANDREW E ET AL: "Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme.", PROTEIN ENGINEERING, vol. 13, no. 5, May 2000 (2000-05-01), pages 323 - 327, XP002182771, ISSN: 0269-2139 * |
WALDO GEOFFREY S ET AL: "Rapid protein-folding assay using green fluorescent protein", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 691 - 695, XP002182769 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9511074B2 (en) | 2009-08-03 | 2016-12-06 | University of Pittsburgh—of the Commonwealth System of Higher Education | Methods of treating disorders associated with protein polymerization |
US9844605B2 (en) * | 2009-11-05 | 2017-12-19 | The University of Pittsburgh—Of the Commonwealth System of Higher Education | Transgenic Caenorhabditis elegans comprising a human protein with a tendency to aggregate fused to a fluorescent protein |
Also Published As
Publication number | Publication date |
---|---|
WO2001060840A2 (fr) | 2001-08-23 |
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