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WO2001088166A2 - Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine - Google Patents

Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine Download PDF

Info

Publication number
WO2001088166A2
WO2001088166A2 PCT/US2001/010820 US0110820W WO0188166A2 WO 2001088166 A2 WO2001088166 A2 WO 2001088166A2 US 0110820 W US0110820 W US 0110820W WO 0188166 A2 WO0188166 A2 WO 0188166A2
Authority
WO
WIPO (PCT)
Prior art keywords
plant
ppm
biotin
binding protein
mean concentration
Prior art date
Application number
PCT/US2001/010820
Other languages
English (en)
Other versions
WO2001088166A3 (fr
Inventor
John Howard
Michele Bailey
Original Assignee
Prodigene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prodigene, Inc. filed Critical Prodigene, Inc.
Priority to CA002409050A priority Critical patent/CA2409050A1/fr
Priority to EP01934862A priority patent/EP1287148A2/fr
Priority to US10/275,272 priority patent/US20030213011A1/en
Priority to AU2001261010A priority patent/AU2001261010A1/en
Publication of WO2001088166A2 publication Critical patent/WO2001088166A2/fr
Publication of WO2001088166A3 publication Critical patent/WO2001088166A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • a second embodiment of the invention is directed to a method of protecting plant tissue, harvested plant material or plant seed against infestation by a plant insect pest or larva thereof, by introducing into the plant's genome a nucleotide sequence encoding a biotin-binding protein, and expressing the sequence to produce the biotin- binding protein at a mean protein concentration that provides an insecticidally effective amount of the biotin-binding protein in plant tissue, harvested plant material or plant seed, wherein the mean protein concentration is > 100 ppm.
  • the insect pest to be controlled by the invention may be one which feeds upon any plant listed above, such as house fly, hide beetle, olive fruit fly, fruit fly, flour mite, European corn borer, tobacco hormworm, maize weevil, lesser grain borer, Angoumois grain moth, warehouse beetle, sawtoothed grain beetle, flat grain beetle, red flour beetle, confused flour beetle, Indianmeal moth, Mediterranean flour moth, corn rootworm, alfalfa weevil, tobacco budworm, beet armyworm, bollworm, sunflower moth, black cutworm and rice weevil.
  • any plant listed above such as house fly, hide beetle, olive fruit fly, fruit fly, flour mite, European corn borer, tobacco hormworm, maize weevil, lesser grain borer, Angoumois grain moth, warehouse beetle, sawtoothed grain beetle, flat grain beetle, red flour beet
  • the vector may also contain such sequences as necessary to allow for the eventual integration of the vector into the chromosome.
  • the expression vector also may contain a gene encoding a selection marker which is functionally linked to promoters that control transcription initiation.
  • a selection marker which is functionally linked to promoters that control transcription initiation.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des méthodes destinées à la lutte contre les infestations par les insectes à l'aide d'une séquence nucléotidique codant pour une protéine de fixation à la biotine pour obtenir des niveaux élevés de protéine de fixation à la biotine dans la plante, c'est-à-dire une concentration moyenne de ≥ 100 ppm.
PCT/US2001/010820 2000-05-17 2001-05-17 Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine WO2001088166A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002409050A CA2409050A1 (fr) 2000-05-17 2001-05-17 Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine
EP01934862A EP1287148A2 (fr) 2000-05-17 2001-05-17 Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine
US10/275,272 US20030213011A1 (en) 2001-05-17 2001-05-17 Insect resistant transgenic plants expressing biontin-binding protein gene
AU2001261010A AU2001261010A1 (en) 2000-05-17 2001-05-17 Controlling insect infestation of transgenic plants containing biotin-binding protein gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20463900P 2000-05-17 2000-05-17
US60/204,639 2000-05-17

Publications (2)

Publication Number Publication Date
WO2001088166A2 true WO2001088166A2 (fr) 2001-11-22
WO2001088166A3 WO2001088166A3 (fr) 2002-06-27

Family

ID=22758787

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010820 WO2001088166A2 (fr) 2000-05-17 2001-05-17 Methodes de lutte contre l'infestation des plantes transgeniques par les insectes a l'aide du gene de la proteine de fixation a la biotine

Country Status (4)

Country Link
EP (1) EP1287148A2 (fr)
AU (1) AU2001261010A1 (fr)
CA (1) CA2409050A1 (fr)
WO (1) WO2001088166A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020055885A1 (fr) * 2018-09-11 2020-03-19 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4671293A (en) * 1992-07-10 1994-01-31 Pioneer Hi-Bred International, Inc. Avidin and homologues as larvicides against insect pests

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020055885A1 (fr) * 2018-09-11 2020-03-19 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation

Also Published As

Publication number Publication date
WO2001088166A3 (fr) 2002-06-27
EP1287148A2 (fr) 2003-03-05
AU2001261010A1 (en) 2001-11-26
CA2409050A1 (fr) 2001-11-22

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