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WO2001088168A3 - Mise en correspondance d'interactions moleculaires dans des plantes et d'analyses de complementation de fragments de proteine - Google Patents

Mise en correspondance d'interactions moleculaires dans des plantes et d'analyses de complementation de fragments de proteine Download PDF

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Publication number
WO2001088168A3
WO2001088168A3 PCT/US2001/015170 US0115170W WO0188168A3 WO 2001088168 A3 WO2001088168 A3 WO 2001088168A3 US 0115170 W US0115170 W US 0115170W WO 0188168 A3 WO0188168 A3 WO 0188168A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
plants
protein fragments
molecular interactions
complementation assays
Prior art date
Application number
PCT/US2001/015170
Other languages
English (en)
Other versions
WO2001088168A2 (fr
Inventor
Stephen W Michnick
Normand Brisson
Original Assignee
Odyssey Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Odyssey Pharmaceuticals Inc filed Critical Odyssey Pharmaceuticals Inc
Priority to AU2001261408A priority Critical patent/AU2001261408A1/en
Publication of WO2001088168A2 publication Critical patent/WO2001088168A2/fr
Publication of WO2001088168A3 publication Critical patent/WO2001088168A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une méthode permettant d'exprimer des partenaires d'interaction pour essais de complémentation de fragments de protéines (PCA) dans un matériau végétal. Cette méthode consiste à : (A) transformer ledit matériau avec (1) une première construction codant pour un premier produit de fusion qui comprend (a) un premier fragment d'une première molécule dont les fragments peuvent présenter une activité détectable lorsqu'ils sont associés et (b) un premier domaine d'interaction protéine-protéine ; et (2) une seconde construction codant pour un second produit de fusion qui comprend (a) un second fragment de ladite première molécule et (b) un second domaine d'interaction protéine-protéine qui peut se lier avec (1)(b) ; (B) cultiver ledit matériau dans des conditions permettent l'expression desdits partenaires d'interaction PCA ; et (C) détecter ladite activité.
PCT/US2001/015170 2000-05-12 2001-05-11 Mise en correspondance d'interactions moleculaires dans des plantes et d'analyses de complementation de fragments de proteine WO2001088168A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001261408A AU2001261408A1 (en) 2000-05-12 2001-05-11 Mapping molecular interactions in plants with protein fragments complementation assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20393700P 2000-05-12 2000-05-12
US60/203,937 2000-05-12

Publications (2)

Publication Number Publication Date
WO2001088168A2 WO2001088168A2 (fr) 2001-11-22
WO2001088168A3 true WO2001088168A3 (fr) 2002-04-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/015170 WO2001088168A2 (fr) 2000-05-12 2001-05-11 Mise en correspondance d'interactions moleculaires dans des plantes et d'analyses de complementation de fragments de proteine

Country Status (2)

Country Link
AU (1) AU2001261408A1 (fr)
WO (1) WO2001088168A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020064769A1 (en) * 2000-10-05 2002-05-30 Watson Michnick Stephen William Dynamic visualization of expressed gene networks in living cells
EP1601576A4 (fr) * 2003-03-03 2007-12-05 Univ Columbia Systemes de biomarquage ligand/partenaire de liaison
BR112013031244B1 (pt) * 2011-06-07 2019-10-29 Fraunhofer Ges Forschung método para a geração de uma linhagem de células de planta monoclonal a partir de uma população heterogênea de células de planta
WO2024103117A1 (fr) * 2022-11-16 2024-05-23 Commonwealth Scientific And Industrial Research Organisation Essai de criblage de protoplastes

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998037211A1 (fr) * 1997-02-21 1998-08-27 Gene Shears Pty. Limited Complementation proteinique dans des plantes transgeniques
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage
WO2000007038A2 (fr) * 1998-07-30 2000-02-10 Universite De Montreal Dosages de complementation proteines-fragments pour la detection d'interactions biologiques ou entre medicaments
WO2000071701A1 (fr) * 1999-05-24 2000-11-30 New England Biolabs, Inc. Procede de generation de genes non transferables separes capables d'exprimer un produit proteique actif
WO2001059091A2 (fr) * 2000-02-11 2001-08-16 Metabolix, Inc. Constructions a expression de genes mutiples contenant des inteines modifiees
WO2001094617A2 (fr) * 2000-06-02 2001-12-13 Odyssey Pharmaceuticals, Inc. Essai de complementation de fragment de proteine a base de beta-lactamase du tem-1 d'e. coli pour la detection des interactions entre proteine et proteine, proteine et petite molecule, et proteine et acide nucleique

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998037211A1 (fr) * 1997-02-21 1998-08-27 Gene Shears Pty. Limited Complementation proteinique dans des plantes transgeniques
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage
WO2000007038A2 (fr) * 1998-07-30 2000-02-10 Universite De Montreal Dosages de complementation proteines-fragments pour la detection d'interactions biologiques ou entre medicaments
WO2000071701A1 (fr) * 1999-05-24 2000-11-30 New England Biolabs, Inc. Procede de generation de genes non transferables separes capables d'exprimer un produit proteique actif
WO2001059091A2 (fr) * 2000-02-11 2001-08-16 Metabolix, Inc. Constructions a expression de genes mutiples contenant des inteines modifiees
WO2001094617A2 (fr) * 2000-06-02 2001-12-13 Odyssey Pharmaceuticals, Inc. Essai de complementation de fragment de proteine a base de beta-lactamase du tem-1 d'e. coli pour la detection des interactions entre proteine et proteine, proteine et petite molecule, et proteine et acide nucleique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Clonal selection and mapping of the functional relationships among plant genes with a protein fragment complementation strategy", 1999-2000 GENOMICS PROJECTS COMPETITION RESULTS - NSERC, XP002189156, Retrieved from the Internet <URL:http://www.nserc.ca/programs/genres00_e.htm> *
PELLETIER, J.F., ET AL: "An in vivo library-versus-library selection of optimized protein-protein interactions", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 663 - 690, XP002189201 *
REMY I ET AL: "CLONAL SELECTION AND IN VIVO QUANTITATION OF PROTEIN INTERACTONS WITH PROTEIN-FRAGMENT COMPLEMENTATION ASSAYS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 10, 11 May 1999 (1999-05-11), pages 5394 - 5399, XP001008795, ISSN: 0027-8424 *
SUBRAMANIAN, R., ET AL.: "Direct visualization of protein interactions in plant cells", NATURE BIOTECHNOLOGY, vol. 19, August 2001 (2001-08-01), pages 769 - 772, XP002189157 *

Also Published As

Publication number Publication date
AU2001261408A1 (en) 2001-11-26
WO2001088168A2 (fr) 2001-11-22

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