[go: up one dir, main page]

WO2001000798A3 - Clonage et expression de cathepsine k humaine dans des cellules mammaliennes - Google Patents

Clonage et expression de cathepsine k humaine dans des cellules mammaliennes Download PDF

Info

Publication number
WO2001000798A3
WO2001000798A3 PCT/US2000/016502 US0016502W WO0100798A3 WO 2001000798 A3 WO2001000798 A3 WO 2001000798A3 US 0016502 W US0016502 W US 0016502W WO 0100798 A3 WO0100798 A3 WO 0100798A3
Authority
WO
WIPO (PCT)
Prior art keywords
cat
cloning
expressing
mammalian cells
human cathepsin
Prior art date
Application number
PCT/US2000/016502
Other languages
English (en)
Other versions
WO2001000798A2 (fr
WO2001000798A9 (fr
Inventor
Mohammad S Saedi
Timothy Hill
Cheryl Schaffer Brehme
Steven J Roman
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Priority to AU61991/00A priority Critical patent/AU6199100A/en
Publication of WO2001000798A2 publication Critical patent/WO2001000798A2/fr
Publication of WO2001000798A3 publication Critical patent/WO2001000798A3/fr
Publication of WO2001000798A9 publication Critical patent/WO2001000798A9/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Cette invention se rapporte à un procédé servant à produire une protéine Cat K recombinée, mature et active comme enzyme, exprimée dans des cellules hôtes mammaliennes, ainsi qu'à un vecteur d'expression permettant d'exprimer de façon stable la protéine Cat K, sous la forme de pCat K, dans un système mammalien. Cette invention se rapporte en outre à des procédés de détection de la protéine Cat K dans un échantillon, ainsi qu'à un kit d'immunodosage et à des procédés d'utilisation de la protéine Cat K recombinée dans le domaine pharmaceutique.
PCT/US2000/016502 1999-06-25 2000-06-15 Clonage et expression de cathepsine k humaine dans des cellules mammaliennes WO2001000798A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU61991/00A AU6199100A (en) 1999-06-25 2000-06-15 Cloning and expressing of human cathepsin k in mammalian cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34422899A 1999-06-25 1999-06-25
US09/344,228 1999-06-25

Publications (3)

Publication Number Publication Date
WO2001000798A2 WO2001000798A2 (fr) 2001-01-04
WO2001000798A3 true WO2001000798A3 (fr) 2001-08-09
WO2001000798A9 WO2001000798A9 (fr) 2002-07-11

Family

ID=23349595

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/016502 WO2001000798A2 (fr) 1999-06-25 2000-06-15 Clonage et expression de cathepsine k humaine dans des cellules mammaliennes

Country Status (2)

Country Link
AU (1) AU6199100A (fr)
WO (1) WO2001000798A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005093421A1 (fr) * 2004-03-10 2005-10-06 Biovendor Laboratory Medicine, Inc. Dosage immuno-enzymatique pour mettre en evidence la cathepsine k

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0445939A1 (fr) * 1990-02-23 1991-09-11 Eli Lilly And Company Unité de contrôle de la transcription modifiée et son usage
WO1996013523A1 (fr) * 1994-10-27 1996-05-09 Khepri Pharmaceuticals, Inc. Protease cathepsine o2
WO1997047642A1 (fr) * 1996-06-14 1997-12-18 Smithkline Beecham Corporation Gene de la cathepsine k

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0445939A1 (fr) * 1990-02-23 1991-09-11 Eli Lilly And Company Unité de contrôle de la transcription modifiée et son usage
WO1996013523A1 (fr) * 1994-10-27 1996-05-09 Khepri Pharmaceuticals, Inc. Protease cathepsine o2
WO1997047642A1 (fr) * 1996-06-14 1997-12-18 Smithkline Beecham Corporation Gene de la cathepsine k

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BROEMME DIETER ET AL: "Human cathepsin O2, a matrix protein-degrading cysteine protease expressed in osteoclasts: Functional expression of human cathepsin O2 in Spodoptera frugiperda and characterization of the enzyme.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 4, 1996, pages 2126 - 2132, XP002162040, ISSN: 0021-9258 *
SAEDI ET AL.: "Recombinant human cathepsin K: expression in mammalian cells, purification, and activation of the enzyme.", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 14, no. Suppl., September 1999 (1999-09-01), pages S356 - Abstr.SA192, XP000982610 *
SHI G -P ET AL: "MOLECULAR CLONING OF HUMAN CATHEPSIN O, A NOVEL ENDOPROTEINASE AND HOMOLOGUE OF RABBIT OC2", FEBS LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 357, 1995, pages 129 - 134, XP002068032, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
WO2001000798A2 (fr) 2001-01-04
WO2001000798A9 (fr) 2002-07-11
AU6199100A (en) 2001-01-31

Similar Documents

Publication Publication Date Title
WO2003076932A3 (fr) Procede de detection et d'analyse d'interactions proteiques in vivo
AU2001278797A1 (en) Expression vector using for animal cell
WO2002029045A3 (fr) Procede de production de proteines dependantes de la vitamine k
WO2001075087A8 (fr) Variantes de lasubtilisine
WO2002006457A3 (fr) Genes de lipase
ES2255281T3 (es) Sobreexpresion de genes de fitasa en sistmas de levadura.
WO2002083890A3 (fr) Utilisation de l'hemolysine clya pour permettre l'excretion de proteines
DE69840165D1 (de) Genkonstrukt das für ein heterologes "prodrug"-aktivierendes enzym und für ein zellgerichtes teil kodiert
BR0008772B1 (pt) vetor de expressão, célula bacteriana ou fúngica cultivada, processos para expandir in vitro células hematopoiéticas e progenitores de células hematopoiéticas, e para detectar a presença de rna de ligando zalfa11 e de ligando zalfa 11 in vitro em uma amostra biológica.
WO2002040697A3 (fr) Decalages du rythme metabolique dans des fermentations exprimant des proteines de recombinaison
IL148785A0 (en) Vectors and methods for recombinant protein expression
AU5136900A (en) Process of expressing and isolating recombinant proteins and recombinant proteinproducts from plants, plant derived tissues or cultured plant cells
NZ514230A (en) A single step method for lysing cells and cleaving a fusion protein
WO1999025878A3 (fr) Procedes pour moduler et identifier la senescence cellulaire
ATE294240T1 (de) Verfahren und kit, um biologische substanzen in plasmamembran und/oder zytosol einzuführen
WO2002036078A3 (fr) Ciblage de cellules souches pluripotentielles dans des tissus
WO2000028031A8 (fr) Nouveaux regulateurs mitogeniques
WO2003021265A8 (fr) Systeme de controle intracellulaire d'un produit de recombinaison genetique
WO1994025590A3 (fr) Thrichohyaline et transglutaminase-3 et procedes d'utilisation
WO2001000798A3 (fr) Clonage et expression de cathepsine k humaine dans des cellules mammaliennes
WO2002081703A3 (fr) Regulateurs d'oxygenase mitogeniques
CA2337088A1 (fr) Techniques et produits de recombinaison relatifs a une expression de proteine
WO2002040645A3 (fr) Constructions cellulaires obtenues a partir de cellules souches mesenchymateuses, cellules pouvant deriver de celles-ci et leur utilisation
WO2004078915A3 (fr) Culture cellulaire
AU2002235771A1 (en) Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: C2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

COP Corrected version of pamphlet

Free format text: PAGES 1/6-6/6, DRAWINGS, REPLACED BY NEW PAGES 1/6-6/6; PAGES 1-3, SEQUENCE LISTING, REPLACED BY NEW PAGES 1-3; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP