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WO2001006004A3 - Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede - Google Patents

Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede Download PDF

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Publication number
WO2001006004A3
WO2001006004A3 PCT/EP2000/006887 EP0006887W WO0106004A3 WO 2001006004 A3 WO2001006004 A3 WO 2001006004A3 EP 0006887 W EP0006887 W EP 0006887W WO 0106004 A3 WO0106004 A3 WO 0106004A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequences
low abundance
amplifying low
abundance nucleic
Prior art date
Application number
PCT/EP2000/006887
Other languages
English (en)
Other versions
WO2001006004A2 (fr
Inventor
Peter Richardson
Peter Cox
Original Assignee
Univ Cambridge Tech
Peter Richardson
Peter Cox
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cambridge Tech, Peter Richardson, Peter Cox filed Critical Univ Cambridge Tech
Priority to CA002378070A priority Critical patent/CA2378070A1/fr
Priority to EP00954533A priority patent/EP1196639A2/fr
Priority to AU66956/00A priority patent/AU6695600A/en
Priority to JP2001511213A priority patent/JP2003505034A/ja
Priority to KR1020027000769A priority patent/KR20020034161A/ko
Publication of WO2001006004A2 publication Critical patent/WO2001006004A2/fr
Publication of WO2001006004A3 publication Critical patent/WO2001006004A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédé ainsi que des amorces d'acide nucléique et des kits les renfermant qui permettent de réaliser une amplification efficace de séquences d'acides nucléiques prises dans un échantillon, plus particulièrement de séquences d'acide nucléiques dont la représentation au sein dudit échantillon est relativement faible au départ.
PCT/EP2000/006887 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede WO2001006004A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002378070A CA2378070A1 (fr) 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede
EP00954533A EP1196639A2 (fr) 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede
AU66956/00A AU6695600A (en) 1999-07-19 2000-07-19 A method for amplifying low abundance nucleic acid sequences and means for performing said method
JP2001511213A JP2003505034A (ja) 1999-07-19 2000-07-19 存在量の少ない核酸配列を増幅する方法および該方法を行うための手段
KR1020027000769A KR20020034161A (ko) 1999-07-19 2000-07-19 불충분한 핵산 서열의 증폭방법 및 그 방법의 수행장치

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14466699P 1999-07-19 1999-07-19
US60/144,666 1999-07-19

Publications (2)

Publication Number Publication Date
WO2001006004A2 WO2001006004A2 (fr) 2001-01-25
WO2001006004A3 true WO2001006004A3 (fr) 2001-08-09

Family

ID=22509592

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006887 WO2001006004A2 (fr) 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede

Country Status (6)

Country Link
EP (1) EP1196639A2 (fr)
JP (1) JP2003505034A (fr)
KR (1) KR20020034161A (fr)
AU (1) AU6695600A (fr)
CA (1) CA2378070A1 (fr)
WO (1) WO2001006004A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1275738A1 (fr) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Procédé pour la synthèse aléatoire et l'amplification d'ADNc
WO2003025202A2 (fr) 2001-09-19 2003-03-27 Alexion Pharmaceuticals, Inc. Matrices transgeniques et leurs utilisations dans l'amplification d'amorce unique
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
GB0216026D0 (en) * 2002-07-10 2002-08-21 Cambridge Biotechnology Ltd Nucleic acid amplification method
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
DK1735459T3 (da) * 2004-04-07 2012-05-29 Exiqon As Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er
KR100590621B1 (ko) * 2004-09-21 2006-06-19 (주)지노믹트리 하이힐 프라이머를 이용한 ｒｎａ의 선형 증폭방법
JP4777631B2 (ja) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
AU2008236678B2 (en) * 2007-04-05 2013-09-12 Genera Biosystems Limited Compositions and methods of detection
CN102685048B (zh) * 2012-05-28 2014-10-29 上海师范大学 一种突发信号盲均衡算法
WO2016082057A1 (fr) * 2014-11-25 2016-06-02 深圳华大基因研究院 Procédé de construction d'une banque de séquençage d'adn libre
CN117305360B (zh) * 2023-09-15 2024-09-24 江南大学 一种哺乳动物细胞核转染液及其制备方法和在细胞核内递送的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (fr) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Reaction en chaine de polymerase specifique de reproduction d'a.r.n. modifiee
WO1998008973A1 (fr) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Amplification globale d'acides nucleiques
WO2000008208A2 (fr) * 1998-08-05 2000-02-17 Medical Research Council Transcription inverse, amplification, et leurs amorces

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015601A1 (fr) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Reaction en chaine de polymerase specifique de reproduction d'a.r.n. modifiee
WO1998008973A1 (fr) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Amplification globale d'acides nucleiques
WO2000008208A2 (fr) * 1998-08-05 2000-02-17 Medical Research Council Transcription inverse, amplification, et leurs amorces

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CHEUNG AND NELSON: "WHOLE GENOME AMPLIFICATION USING A DEGENERATE OLIGONUCLEOTIDE PRIMER ALLOWS HUNDREDS OF GENOTYPES TO BE PERFORMED ON LESS THAN ONE NANOGRAM OF GENOMIC DNA", PNAS, vol. 93, 1996, pages 14676 - 14679, XP002128316 *
DIXON ET AL.: "EXPRESSION PROFILING OF SINGLE CELLS USING 3 PRIME END AMPLIFICATION (TPEA) PCR", NUCLEIC ACIDS RESEARCH, vol. 26, no. 19, October 1998 (1998-10-01), pages 4426 - 4431, XP002128317 *
EBERWINE J ET AL: "ANALYSIS OF GENE EXPRESSION IN SINGLE LIVE NEURONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 89, 1 April 1992 (1992-04-01), pages 3010 - 3014, XP002912945, ISSN: 0027-8424 *
FROUSSARD: "A RANDOM-PCR METHOD (rPCR) TO CONSTRUCT WHOLE cDNA LIBRARY FROM LOW AMOUNTS OF RNA", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, pages 2900, XP002128315 *
RICHARDSON ET AL.: "RELATING GENE EXPRESSION TO CELL FUNCTION USING A NOVEL SINGLE CELL PCR METHOD", EUROPEAN JOURNAL OF NEUROSCIENCE, vol. 10, no. supp. [10], August 1998 (1998-08-01), pages 10307, XP000982404 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718-725, XP000199116, ISSN: 0888-7543 *
ZHAO ET AL.: "3'-END cDNA POOL SUITABLE FOR DIFFERENTIAL DISPLAY FROM A SMALL NUMBER OF CELLS", BIOTECHNIQUES, vol. 24, May 1998 (1998-05-01), pages 842 - 852, XP002128314 *
ZHAO ET AL.: "NEW PRIMER STRATEGY IMPROVES PRECISION OF DIFFERENTIAL DISPLAY", BIOTECHNIQUES, vol. 18, no. 5, 1995, pages 842 - 850, XP002128267 *

Also Published As

Publication number Publication date
KR20020034161A (ko) 2002-05-08
EP1196639A2 (fr) 2002-04-17
WO2001006004A2 (fr) 2001-01-25
CA2378070A1 (fr) 2001-01-25
AU6695600A (en) 2001-02-05
JP2003505034A (ja) 2003-02-12

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