WO2001006008A3 - Deplacement de brins multiplexes pour determinations d'acide nucleique - Google Patents
Deplacement de brins multiplexes pour determinations d'acide nucleique Download PDFInfo
- Publication number
- WO2001006008A3 WO2001006008A3 PCT/US2000/018301 US0018301W WO0106008A3 WO 2001006008 A3 WO2001006008 A3 WO 2001006008A3 US 0018301 W US0018301 W US 0018301W WO 0106008 A3 WO0106008 A3 WO 0106008A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- determinations
- multiplexed
- oligonucleotides
- release
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 238000006073 displacement reaction Methods 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Des déterminations multiplexées de grands nombres d'événements sont obtenues d'une manière précise et simple à l'aide d'une combinaison de différents oligonucléotides, lesquels servent à la fois à la capture et à la libération sur et à partir d'un support, et des oligonucléotides de déplacement de brins destinés à la libération séquentielle de groupes d'oligonucléotides. Sont également compris, faisant partie des réactifs oligonucléotidiques, des identificateurs, lesquels servent à identifier une caractéristique particulière. La méthode est illustrée à l'aide d'amorces pour l'amorçage de prolongement de chaîne joints à ou servant de séquence de capture/libération, l'amorce prolongée ayant un identificateur. Après prolongement de l'amorce, les amorces prolongées sont capturées et libérées indépendamment et les amorces prolongées libérées sont dosées. La méthode de l'invention trouve une application dans des déterminations de séquences d'acide nucléique, des déterminations de polymorphismes mononucléotidiques, l'identification de fragments d'acide nucléique et analogue.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002379749A CA2379749A1 (fr) | 1999-07-16 | 2000-06-30 | Deplacement de brins multiplexes pour determinations d'acide nucleique |
| EP00948570A EP1200624A2 (fr) | 1999-07-16 | 2000-06-30 | Deplacement de brins multiplexes pour determinations d'acide nucleique |
| JP2001511216A JP2003505036A (ja) | 1999-07-16 | 2000-06-30 | 核酸決定のための多重鎖置換 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35462999A | 1999-07-16 | 1999-07-16 | |
| US09/354,629 | 1999-07-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2001006008A2 WO2001006008A2 (fr) | 2001-01-25 |
| WO2001006008A3 true WO2001006008A3 (fr) | 2001-07-12 |
Family
ID=23394232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2000/018301 WO2001006008A2 (fr) | 1999-07-16 | 2000-06-30 | Deplacement de brins multiplexes pour determinations d'acide nucleique |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1200624A2 (fr) |
| JP (1) | JP2003505036A (fr) |
| CA (1) | CA2379749A1 (fr) |
| WO (1) | WO2001006008A2 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001296668A1 (en) * | 2000-10-05 | 2002-04-15 | Aclara Biosciences, Inc. | Multiplexed differential displacement for nucleic acid determinations |
| JP4839051B2 (ja) * | 2004-10-08 | 2011-12-14 | シスメックス株式会社 | 核酸プローブを用いる被検物質の検出方法 |
| DE602005018316D1 (de) * | 2004-10-08 | 2010-01-28 | Sysmex Corp | Verfahren zum Nachweis einer Zielsubstanz unter Verwendung einer Nukleinsäuresonde |
| US20240209429A1 (en) * | 2022-12-07 | 2024-06-27 | Illumina, Inc. | Monoclonal clustering using double stranded dna size exclusion with patterned seeding |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987003911A1 (fr) * | 1985-12-17 | 1987-07-02 | Genetics Institute, Inc. | Procede d'analyse de polynucleotides base sur le deplacement des brins, et complexe reactif |
| EP0269764A1 (fr) * | 1986-12-01 | 1988-06-08 | Molecular Biosystems, Inc. | Procédé pour augmenter la sensibilité des essais d'hybridation des acides nucléiques |
| US4818680A (en) * | 1985-12-18 | 1989-04-04 | Mary Collins | Method and kit involving displacement and rehybridization of labeled polynucleotide |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| WO1998014610A2 (fr) * | 1996-10-04 | 1998-04-09 | The Perkin-Elmer Corporation | Procedes et compositions de capture de polynucleotides multiplex |
| EP0871774A1 (fr) * | 1995-07-25 | 1998-10-21 | University Of Strathclyde | Detection d'acides nucleiques et d'unites d'acides nucleiques |
| WO1999024612A2 (fr) * | 1997-11-06 | 1999-05-20 | Mosaic Technologies | Reactions multiples de deplacement polynucleotidique sequentiel pour amplification et traitement de signaux |
-
2000
- 2000-06-30 WO PCT/US2000/018301 patent/WO2001006008A2/fr not_active Application Discontinuation
- 2000-06-30 EP EP00948570A patent/EP1200624A2/fr not_active Withdrawn
- 2000-06-30 JP JP2001511216A patent/JP2003505036A/ja not_active Withdrawn
- 2000-06-30 CA CA002379749A patent/CA2379749A1/fr not_active Abandoned
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987003911A1 (fr) * | 1985-12-17 | 1987-07-02 | Genetics Institute, Inc. | Procede d'analyse de polynucleotides base sur le deplacement des brins, et complexe reactif |
| US4818680A (en) * | 1985-12-18 | 1989-04-04 | Mary Collins | Method and kit involving displacement and rehybridization of labeled polynucleotide |
| EP0269764A1 (fr) * | 1986-12-01 | 1988-06-08 | Molecular Biosystems, Inc. | Procédé pour augmenter la sensibilité des essais d'hybridation des acides nucléiques |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| EP0871774A1 (fr) * | 1995-07-25 | 1998-10-21 | University Of Strathclyde | Detection d'acides nucleiques et d'unites d'acides nucleiques |
| WO1998014610A2 (fr) * | 1996-10-04 | 1998-04-09 | The Perkin-Elmer Corporation | Procedes et compositions de capture de polynucleotides multiplex |
| WO1999024612A2 (fr) * | 1997-11-06 | 1999-05-20 | Mosaic Technologies | Reactions multiples de deplacement polynucleotidique sequentiel pour amplification et traitement de signaux |
Non-Patent Citations (1)
| Title |
|---|
| WOLCOTT M J: "ADVANCES IN NUCLEIC ACID-BASED DETECTION METHODS", CLINICAL MICROBIOLOGY REVIEWS,US,WASHINGTON, DC, vol. 5, no. 4, 1 October 1992 (1992-10-01), pages 370 - 386, XP000606216, ISSN: 0893-8512 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2379749A1 (fr) | 2001-01-25 |
| EP1200624A2 (fr) | 2002-05-02 |
| JP2003505036A (ja) | 2003-02-12 |
| WO2001006008A2 (fr) | 2001-01-25 |
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