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WO2002004598A3 - Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase - Google Patents

Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase Download PDF

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Publication number
WO2002004598A3
WO2002004598A3 PCT/US2001/021864 US0121864W WO0204598A3 WO 2002004598 A3 WO2002004598 A3 WO 2002004598A3 US 0121864 W US0121864 W US 0121864W WO 0204598 A3 WO0204598 A3 WO 0204598A3
Authority
WO
WIPO (PCT)
Prior art keywords
inhibitors
pyruvate dehydrogenase
improvement
cell culture
dehydrogenase kinase
Prior art date
Application number
PCT/US2001/021864
Other languages
French (fr)
Other versions
WO2002004598A2 (en
Inventor
Brian D Follstad
Original Assignee
Immunex Corp
Brian D Follstad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corp, Brian D Follstad filed Critical Immunex Corp
Priority to AU2001275897A priority Critical patent/AU2001275897A1/en
Publication of WO2002004598A2 publication Critical patent/WO2002004598A2/en
Publication of WO2002004598A3 publication Critical patent/WO2002004598A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides methods and compositions for improved in vitro culture of animal cells that make use of inhibitors of pyruvate dehydrogenase kinase. Particularly preferred inhibitors are salts of dichloroacetic acid such as sodium dichloroacetate.
PCT/US2001/021864 2000-07-12 2001-07-10 Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase WO2002004598A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001275897A AU2001275897A1 (en) 2000-07-12 2001-07-10 Improvement of cell culture performance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21788900P 2000-07-12 2000-07-12
US60/217,889 2000-07-12

Publications (2)

Publication Number Publication Date
WO2002004598A2 WO2002004598A2 (en) 2002-01-17
WO2002004598A3 true WO2002004598A3 (en) 2002-04-04

Family

ID=22812901

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021864 WO2002004598A2 (en) 2000-07-12 2001-07-10 Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase

Country Status (2)

Country Link
AU (1) AU2001275897A1 (en)
WO (1) WO2002004598A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939632B2 (en) 2006-06-14 2011-05-10 Csl Behring Gmbh Proteolytically cleavable fusion proteins with high molar specific activity
US8754194B2 (en) 2006-12-22 2014-06-17 Csl Behring Gmbh Modified coagulation factors with prolonged in vivo half-life
US8765915B2 (en) 2006-02-06 2014-07-01 Csl Behring Gmbh Modified coagulation factor VIIa with extended half-life
US9487809B2 (en) 2010-05-28 2016-11-08 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005325768A1 (en) * 2005-01-25 2006-08-03 Apollo Life Sciences Limited Molecules and chimeric molecules thereof
CA2655248C (en) 2006-06-14 2016-02-09 Csl Behring Gmbh Proteolytically cleavable fusion protein comprising a blood coagulation factor
EP1867660A1 (en) 2006-06-14 2007-12-19 CSL Behring GmbH Proteolytically cleavable fusion protein comprising a blood coagulation factor
US8637312B2 (en) 2008-01-09 2014-01-28 Cellca Gmbh Mammalian culture media with polyamine and iron
KR101040486B1 (en) 2008-08-25 2011-06-09 경북대학교 산학협력단 New Uses for PD4

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEBERNITZ GREGORY R ET AL: "Anilides of (R)-trifluoro-2-hydroxy-2-methylpropionic acid as inhibitors of pyruvate dehydrogenase kinase.", JOURNAL OF MEDICINAL CHEMISTRY, vol. 43, no. 11, 1 June 2000 (2000-06-01), pages 2248 - 2257, XP001026040, ISSN: 0022-2623 *
MURRAY K ET AL: "DICHLOROACETATE INCREASES CELL AND ANTIBODY YIELDS IN BATCH CULTURES OF A HYBRIDOMA CELL LINE", BIOTECHNOLOGY AND BIOENGINEERING. INCLUDING: SYMPOSIUM BIOTECHNOLOGY IN ENERGY PRODUCTION AND CONSERVATION, JOHN WILEY & SONS. NEW YORK, US, vol. 49, no. 4, 20 February 1996 (1996-02-20), pages 377 - 382, XP001022682, ISSN: 0006-3592 *
YOUNG P R ET AL: "SECRETION OF LACTIC ACID BY PERITONEAL MACROPHAGES DURING EXTRACELLULAR PHAGOCYTOSIS THE POSSIBLE ROLE OF LOCAL HYPERACIDITY IN INFLAMMATORY DEMYELINATION", JOURNAL OF NEUROIMMUNOLOGY, vol. 15, no. 3, 1987, pages 295 - 308, XP001022668, ISSN: 0165-5728 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8765915B2 (en) 2006-02-06 2014-07-01 Csl Behring Gmbh Modified coagulation factor VIIa with extended half-life
US7939632B2 (en) 2006-06-14 2011-05-10 Csl Behring Gmbh Proteolytically cleavable fusion proteins with high molar specific activity
US8754194B2 (en) 2006-12-22 2014-06-17 Csl Behring Gmbh Modified coagulation factors with prolonged in vivo half-life
US9487809B2 (en) 2010-05-28 2016-11-08 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
US10011856B2 (en) 2010-05-28 2018-07-03 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
US10704071B2 (en) 2010-05-28 2020-07-07 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by down regulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
US11046987B2 (en) 2010-05-28 2021-06-29 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase

Also Published As

Publication number Publication date
WO2002004598A2 (en) 2002-01-17
AU2001275897A1 (en) 2002-01-21

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