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WO2002013849A1 - Utilisation de la colostrinine, de ses peptides constitutifs et de ses analogues pour activer des cytokines - Google Patents

Utilisation de la colostrinine, de ses peptides constitutifs et de ses analogues pour activer des cytokines Download PDF

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Publication number
WO2002013849A1
WO2002013849A1 PCT/US2000/022775 US0022775W WO0213849A1 WO 2002013849 A1 WO2002013849 A1 WO 2002013849A1 US 0022775 W US0022775 W US 0022775W WO 0213849 A1 WO0213849 A1 WO 0213849A1
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WIPO (PCT)
Prior art keywords
seq
cell
regulator
group
combinations
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PCT/US2000/022775
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English (en)
Inventor
G. John Stanton
Thomas K. Hughes, Jr.
Istvan Boldogh
Jerzy Georgiades
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The University Of Texas System
Regen Therapeutics Plc
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Application filed by The University Of Texas System, Regen Therapeutics Plc filed Critical The University Of Texas System
Priority to PCT/US2000/022775 priority Critical patent/WO2002013849A1/fr
Priority to AU2000267883A priority patent/AU2000267883A1/en
Publication of WO2002013849A1 publication Critical patent/WO2002013849A1/fr

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08Peptides having 5 to 11 amino acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/10Peptides having 12 to 20 amino acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans

Definitions

  • Colostrum is a component of the milk of mammals during the first few days after birth. Colostrum is a thick yellowish fluid and is the first lacteal secretion post parturition and contains a high concentration of immunogloblins (IgG, IgM, and IgA) and a variety of non-specific proteins. Colostrum also contains various cells such as granular and stromal cells, neutrophils, monocyte/macrophages, and lymphocytes. Colostrum also includes growth factors, hormones, and cytokines. Unlike mature breast milk, colostrum contains low sugar, low iron, but is rich is lipids, proteins, mineral salts, vitamins, and irnmunoglobins.
  • IgG immunogloblins
  • IgM immunogloblins
  • IgA immunogloblins
  • Colostrum also contains various cells such as granular and stromal cells, neutrophils, monocyte/macrophages, and lymphocytes. Colostrum also includes growth factors, hormones, and
  • Colostrum also includes or contains a proline-rich polypeptide aggregate, which is referred to as colostrinin.
  • colostrinin One peptide fragment of colostrinin is Val- Glu-Ser-Tyr-Val-Pro-Leu-Phe-Pro (SEQ ID NO:31), which is disclosed in International Publication No. WO-A-98/14473.
  • Colostrinin and this fragment have been identified as useful in the treatment of disorders of the central nervous system, neurological disorders, mental disorders, dementia, neurodegenerative diseases, Alzheimer's disease, motor neurone disease, psychosis, neurosis, chronic disorders of the immune system, diseases with a bacterial and viral aetiology, and acquired immunological deficiencies as set forth in International Publication No.
  • the present invention relates to the use of colostrinin, at least one constituent (i.e., component) peptide thereof, at least one active analog thereof (e.g., peptide having an N-terminal sequence equivalent to an -terminal sequence of at least one of the colostrinin constituent peptides), and combinations thereof, as a cytokine-inducing agent.
  • These agents can be used as immunological regulators to modulate (e.g., enhance, inhibit, modify, augment, or otherwise alter, and preferably promote) specific or nonspecific immune responses in patients, particularly animals including mammals such as humans.
  • They can also be used as blood cell regulators to modulate (e.g., enhance, inhibit, modify, augment, or otherwise alter, preferably, and promote) cellular proliferation or differentiation (preferably, promoting proliferation and differentiation) of blood cells, such as leukocytes.
  • the present invention provides a method of inducing a cytokine in a cell.
  • the method includes contacting the cell with an immunological regulator under conditions effective to induce (i.e., induce the synthesis or production of) at least one cytokine (either directly or indirectly), wherein the immunological regulator is selected from the group of MQPPPLP (SEQ ID NO.l); LQTPQPLLQVMMEPQGD (SEQ ID NO:2); DQPPDVEKPDLQPFQVQS (SEQ ID NO:3); LFFFLPWNVLP (SEQ ID NO:4); DLEMPVLPVEPFPFV (SEQ ID NO:5); MPQNFYKLPQM (SEQ ID NO:6); NLEMKFPPPPQETVT (SEQ ID ⁇ O:7); LKPFPKLKVEVFPFP (SEQ ID NO:8); WMEV (SEQ ED NO:9); SEQP (SEQ ID NO: 10); DKE (SEQ ID NO:l)
  • VLPPNVG (SEQ ID NO:17); VYPFTGPIPN (SEQ ID NO:18); SLPQNILPL (SEQ ID NO: 19); TQTPVWPPF (SEQ ID NO:20);
  • LQPEIMGVPKVKETMVPK (SEQ ID NO:21); HKEMPFPKYPVEPFTESQ (SEQ ID NO:22); SLTLTDVEKLHLPLPLVQ (SEQ ID NO:23); SWMHQPP (SEQ ID NO:24); QPLPPTNMFP (SEQ ID ⁇ O:25); PQSNLS (SEQ ID ⁇ O:26); LSQPKNLPVPQKAVPQRDMPIQ (SEQ ID ⁇ O:27); AFLLYQE (SEQ ID NO:28); RGPFPILV (SEQ ID NO:29); ATFNRYQDDHGEEILKSL (SEQ ID NO:30); FLLYQEPVLGPNR (SEQ ID ⁇ O:32); LNF (SEQ ID NO:33); and MHQPPQPLPPTNMFP (SEQ ID ⁇ O:34); an active analog thereof; and combinations thereof; with the proviso that the immunological regulator is not VESYVPLFP (SEQ ID NO:31).
  • the cell can be in a cell culture, a tissue, an organ, or an organism. Hence, this method can be carried out in vivo or in vitro.
  • a method for modulating an immune response in a cell includes contacting the cell with an immunological regulator under conditions effective to induce at least one cytokine, wherein the immunological regulator is listed above.
  • the cell can be in a cell culture, a tissue, an organ, or an organism. Hence, this method can be carried out in vivo or in vitro.
  • a method for modulating an immune response in a patient includes administering to the patient an immunological regulator under conditions effective to induce at least one cytokine, wherein the immunological regulator is listed above.
  • the immune response can be specific or nonspecific.
  • one or more cytokines are directly induced using the polypeptides described herein, which then results in an upregulation or a downregulation of one or more other cytokines.
  • various cytokine profiles and immune responses can be produced, which may be specific or nonspecific. Examples of such immune responses include the interferon response and antibody production.
  • at least one cytokine level increases, whether it be increased as a result of direct inducement by one of the peptides described herein, or as a result of indirect inducement (e.g., through the interaction with another cytokine), a peptide is "active" as used herein.
  • a method for modulating blood cell proliferation includes contacting blood cells with a blood cell regulator selected from the group of colostrinin, a constituent peptide thereof, an analog thereof, and combinations thereof, under conditions effective to change the number of blood cells.
  • the blood cells can be in a cell culture or an organism. Hence, this method can be carried out in vivo or in vitro.
  • a method for modulating blood cell proliferation in a patient includes administering to the patient a blood cell regulator selected from the group of colostrinin, a constituent peptide thereof, an analog thereof, and combinations thereof, under conditions effective to change the number of blood cells.
  • the blood cells can be mammalian blood cells, such as human blood cells.
  • the blood cells are increased in number, although a decrease in number can also be desirable in certain situations such as leukemia, myelopathy, etc. More preferably, the blood cells are increased in number and differentiated.
  • the blood cell regulator is preferably a constituent peptide of colostrinin.
  • the invention provides the use of an immunological regulator or blood cell regulator in the manufacture of a medicament for use in the methods described herein.
  • the present invention also provides an immune-inducing composition that includes a pharmaceutical carrier and an active agent selected from the MQPPPLP (SEQ ID NO:l); LQTPQPLLQVMMEPQGD (SEQ ID NO:2); DQPPDVEKPDLQPFQVQS (SEQ ID NO:3); LFFFLPVVNVLP (SEQ ID NO:4); DLEMPVLPVEPFPFV (SEQ ID NO:5); MPQNFYKLPQM (SEQ ID NO:6); VLEMKFPPPPQETVT (SEQ ID NO:7); LKPFPKLKVEVFPFP (SEQ ID NO:8); VNMEN (SEQ ID ⁇ O:9); SEQP (SEQ ID NO: 10); DKE (SEQ ID NO:l 1); FPPPK (SEQ ID NO:12); DSQPPV (SEQ ID NO:13); DPPPPQS (SEQ ID NO:14); SEEMP (SEQ ID NO:15); KYKLQPE (S
  • compositions and methods of the invention means one or more (or at least one), such that combinations of active agents (i.e., active immunological regulators or blood cell differentiation promoters), for example, can be used in the compositions and methods of the invention.
  • active agents i.e., active immunological regulators or blood cell differentiation promoters
  • a composition that includes "a" polypeptide refers to a composition that includes one or more polypeptides.
  • amino acid is used herein to refer to a chemical compound with the general formula: NH 2 — CRH — COOH, where R, the side chain, is H or an organic group. Where R is organic, R can vary and is either polar or nonpolar (i.e., hydrophobic). The amino acids of this invention can be naturally occurring or synthetic (often referred to as nonproteinogenic).
  • an organic group is a hydrocarbon group that is classified as an aliphatic group, a cyclic group or combination of aliphatic and cyclic groups.
  • aliphatic group means a saturated or unsaturated linear or branched hydrocarbon group.
  • cyclic group means a closed ring hydrocarbon group that is classified as an alicyclic group, aromatic group, or heterocyclic group.
  • alicyclic group means a cyclic hydrocarbon group having properties resembling those of aliphatic groups.
  • aromatic group refers to mono- or polycyclic aromatic hydrocarbon groups.
  • an organic group can be substituted or unsubstituted.
  • polypeptide and “peptide” are used interchangeably herein to refer to a polymer of amino acids. These terms do not connote a specific length of a polymer of amino acids. Thus, for example, the terms oligopeptide, protein, and enzyme are included within the definition of polypeptide or peptide, whether produced using recombinant techniques, chemical or enzymatic synthesis, or naturally occurring. This term also includes polypeptides that have been modified or derivatized, such as by glycosylation, acetylation, phosphorylation, and the like. The following abbreviations are used throughout the application:
  • colostrinin at least one constituent (i.e..- component) peptide thereof, and/or at least one active analog thereof (e.g., a peptide having an N-terminal sequence equivalent to an N-terminal sequence of at least one of the colostrinin constituent peptides)
  • at least one cytokine e.g., TNF- ⁇ , IFN- ⁇ , IL-1, IL-2, IL-4, EL-6, 1-10, IL-12.
  • the cytokine can be either directly or indirectly induced.
  • an immune response or blood cell proliferation or differentiation preferably, the promotion of blood cell proliferation, and more preferably, the promotion of blood cell proliferation and differentiation
  • Such immunological regulators and blood cell regulators are referred to herein as "active agents.”
  • active agents can be administered alone or in various combinations to a patient (e.g., animals including humans) as a medication or dietary (e.g., nutrient) supplement in a dose sufficient to modulate one or more immune responses throughout the patient's body, in a specific tissue site, or in a collection of tissue sites.
  • IFN- ⁇ is a potent immunomodulater that is important for the development of the cytotoxic lymphocyte response (CTL).
  • CTL cytotoxic lymphocyte response
  • This immune response is considered to be very important in protecting humans and animals from a variety of bacterial, viral, parasitic, and fungal diseases.
  • TNF- ⁇ is also induced is important because TNF- ⁇ is a major activator of macrophages, among other immune cells, which are important in host defense against infections, hi addition, TNF- ⁇ has been shown to have activity against cancer, directly through its lytic activity and indirectly through macrophages.
  • IL-10 is another important immune mediator that controls both EFN- ⁇ and TNF- ⁇ production and action. Its production represent a negative feedback control for IFN- ⁇ and TNF- ⁇ production. Another one of its hallmark activities is the control of antibody production during the humoral immune responses, which is certainly important in many types of infections. In addition to E -10's immune activities, it also has been shown to play a role in the neuroendocrine system by modulating certain stress responses and immune responses. EL- 10 has been shown to induce the production of corticotropin from pitutitary cells. Corticotropin works downstreanm in the hypothalmic adrenal axis to induce glucocortico steroids that are inherently immunomodulatory.
  • the EL-4 is important in the development of B cell responses, which are the mediators of the humoral immune response.
  • the EL- 12 is an important IFN- ⁇ inducer.
  • compositions of the present invention can be utilized to control immunological and blood cell differentiating activity.
  • active agents described herein can be used individually, in various combinations, or combined with other previously known or newly invented pharmacological agents, such as antioxidants. They can be used as adjuvants for existing vaccinations as well.
  • the present invention provides a method for modulating an immune response. Whether it be in vivo or in vitro, this method involves monitoring the level of at least one cytokine, which can be done by known methods, such as disclosed by Inglot et al., Arch. Immunol. Ther. Exp.. 44, 215-224 (1996); Blach-Olszewska et al., Arch. Immunol. Ther. Exp..45, 43- 47 (1997); Piasecki et al., Arch. Immunol. Ther. Exp..45, 109-117 (1997); Hughes et al., Int. J. Immunopharmacol.. 17. 857-863 (1995); and Mishell et al., Selected Methods in Cellular Immunology. W.H. Freeman, 1980. Specific in vitro methods are described in the Examples Section.
  • the present invention provides a method for modulating blood cell proliferation (preferably, proliferation and differentiation). Whether it be in vivo or in vitro, this method involves monitoring the level of increase or decrease in the number of blood cells bearing a specific phenotypic marker (for differentiation, the types of cells formed are evaluated), as disclosed by Kim et al., Clin. Lab. Haematol..20, 21-29 (1998); Grunwald et al., Methods Mol. Biol.. 119. 443-454 (1999); Villas et al., C L Vis.. 5, 56-61 (1998); and Gratama et al., Cytometry. 33, 166-178 (1998). Specific in vitro methods are described in the Examples Section.
  • Colostrinin is composed of peptides, the aggregate of which has a molecular weight range between about 5.8 to about 26 kiloDaltons (kDa) determined by polyacrylamide gel electrophoresis. It has a greater concentration of proline than any other amino acid.
  • Ovine colostrinin has been found to have a molecular weight of about 18 kDa and includes three non-covalently linked subunits having a molecular weight of about 6 kDa and has about 22 wt-% proline.
  • Ovine colostrinin has also been shown to contain the following number of residues per subunit: lysine - 2; histidine - 1; arginine - 0; aspartic acid - 2; threonine - 4; serine - 3; glutamic acid - 6; proline - 11; glycine - 2; alanine - 0; valine - 5; methionine - 2; isoleucine - 2; leucine - 6; tyrosine - 1; phenylalanine - 3; and cysteine - 0.
  • Colostrinin has been found to include a number of peptides ranging from 3 amino acids to 22 amino acids or more. These can be obtained by various known techniques, including isolation and purification involving eletrophoresis and synthetic techniques. The specific method of obtaining colostrinin and SEQ ID NO:31 is described in International Publication No. WO-A-98/14473. Using HPLC and Edelman Degradation, over 30 constituent peptides of colostrinin have been identified, which can be classified into several groups: (A) those of unknown precursor; (B) those having a ⁇ -casein homologue precursor; (C) those having a ⁇ -casein precursor; and (D) those having an annexin precursor.
  • A those of unknown precursor
  • B those having a ⁇ -casein homologue precursor
  • C those having a ⁇ -casein precursor
  • D those having an annexin precursor.
  • These peptides are described in International Patent Application PCT/GB00/02128, filed June 2, 2000, claiming priority to June 2, 1999, and can be synthesized according to the general method described in the Examples Section.
  • These peptides i.e., constituent peptides of colostrinin
  • MQPPPLP SEQ ID NO:l
  • LQTPQPLLQVMMEPQGD SEQ ID NO:2
  • DQPPDVEKPDLQPFQVQS SEQ ID NO:3
  • LFFFLPWNVLP SEQ ID NO:4
  • DLEMPVLPVEPFPFV SEQ ED NO:5
  • MPQNFYKLPQM SEQ ID NO:6
  • VLEMKFPPPPQETVT SEQ ID NO:7
  • LKPFPKLKVEVFPFP SEQ ID NO:8
  • VNMEN SEQ ID ⁇ O:9
  • SEQP SEQ ID NO:9
  • TQTPWVPPF (SEQ ID NO:20); LQPEMGVPKVKETMVPK (SEQ ID NO:21); HKEMPFPKYPVEPFTESQ (SEQ ID NO:22); SLTLTDVEKLHLPLPLVQ (SEQ ID NO:23); SWMHQPP (SEQ ID NO:24); QPLPPTVMFP (SEQ ID NO:25); PQSVLS (SEQ ED NO:26); LSQPKVLPVPQKAVPQRDMPIQ (SEQ ID NO:27); AFLLYQE (SEQ ID NO:28); RGPFPEN (SEQ ID ⁇ O:29); ATFNRYQDDHGEEELKSL (SEQ ED NO:30); NESYNPLFP (SEQ ID ⁇ O:31); FLLYQEPNLGPNR (SEQ ID ⁇ O:32); LNF (SEQ ID NO:33); and MHQPPQPLPPTVMFP (SEQ ID NO:34).
  • those of unknown precursor include SEQ ID NOs:2, 6, 7, 8, 10, 11, 14, and 33;
  • those having a ⁇ -casein homologue precursor include SEQ ID NOs.T, 3, 4, 5, 9, 12, 13, 15, 16, 17, and 31;
  • those having a ⁇ -casein precursor include SEQ ID NOs:18 (casein amino acids 74-83), 19 (casein amino acids 84-92), 20 (casein amino acids 93-102), 21 (casein amino acids 103-120), 22 (casein amino acids 121-138), 23 (casein amino acids 139-156), 24 (casein amino acids 157-163), 25 (casein amino acids 164-173), 26 (casein amino acids 174-179), 27 (casein amino acids 180-201), 28 (casein amino acids 202-208), 29 (casein amino acids 214-222), 32 (casein amino acids 203-214), and 34 (casein amino acids 159-173); and (D) those having an annexin precursor include SEQ ID NOs:18 (casein amino acids 74
  • a preferred group of such peptides does not include SEQ ID NO:31.
  • a more preferred group of such peptides includes: MQPPPLP (SEQ ID NO:l); LQTPQPLLQVMMEPQGD (SEQ ID NO:2); DQPPDVEKPDLQPFQVQS (SEQ ID NO:3); LFFFLPWNVLP (SEQ ID NO:4); DLEMPNLPNEPFPFV (SEQ ED ⁇ O:5); MPQNFYKLPQM (SEQ ID NO:6); VLEMKFPPPPQETVT (SEQ ID NO:7); LKPFPKLKVEVFPFP (SEQ ID NO:8); VYPFTGPIPN (SEQ ID NO:18), SLPQNILPL (SEQ ID NO:19), TQTPVWPPF (SEQ ID NO:20), HKEMPFPKYPVEPFTESQ (SEQ ID NO:22), and combinations thereof.
  • polypeptides of SEQ ID NOs: 1-34 can be in their free acid form or they can be amidated at the C-terminal carboxylate group.
  • the present invention also includes analogs of the polypeptides of SEQ ID NOs : 1 -34, which includes polypeptides having structural similarity with SEQ ID NOs: 1-34. These peptides can also form a part of a larger peptide.
  • An "analog" of a polypeptide includes at least a portion of the polypeptide, wherein the portion contains deletions or additions of one or more contiguous or noncontiguous amino acids, or containing one or more amino acid substitutions.
  • an “analog” can thus include additional amino acids at one or both of the terminii of the polypeptides listed above.
  • Substitutes for an amino acid in the polypeptides of the invention are preferably conservative substitutions, which are selected from other members of the class to which the amino acid belongs. For example, it is well-known in the art of protein biochemistry that an amino acid belonging to a grouping of amino acids having a particular size or characteristic (such as charge, hydrophobicity and hydrophilicity) can generally be substituted for another amino acid without substantially altering the structure of a polypeptide.
  • conservative amino acid substitutions are defined to result from exchange of amino acids residues from within one of the following classes of residues: Class I: Ala, Gly, Ser, Thr, and Pro (representing small aliphatic side chains and hydroxyl group side chains); Class II: Cys, Ser, Thr and Tyr (representing side chains including an -OH or -SH group); Class HI: Glu, Asp, Asn and Gin (carboxyl group containing side chains): Class IV: His, Arg and Lys (representing basic side chains); Class V: He, Val, Leu, Phe and Met (representing hydrophobic side chains); and Class VI: Phe, T ⁇ , Tyr and His (representing aromatic side chains).
  • the classes also include related amino acids such as 3Hyp and 4Hyp in Class I; homocysteine in Class II; 2-aminoadipic acid, 2-aminopimelic acid, ⁇ -carboxyglutamic acid, ⁇ - carboxyaspartic acid, and the corresponding amino acid amides in Class HI; ornithine, homoarginine, N-methyl lysine, dimethyl lysine, trimethyl lysine, 2,3- diaminopropionic acid, 2,4-diaminobutyric acid, homoarginine, sarcosine and hydroxylysine in Class IV; substituted phenylalanines, norleucine, norvaline, 2- aminooctanoic acid, 2-aminoheptanoic acid, statine and ⁇ -valine in Class V; and naphthylalanines, substituted phenylalanines, tetrahydroisoquinoline-3- carboxylic acid, and halogenated
  • active analogs of colostrinin and its constituent peptides include polypeptides having a relatively large number of proline residues.
  • a "large number" preferably means that a polypeptide includes at least about 15% proline (by number), and more preferably at least about 20% proline (by number).
  • active analogs include more proline residues than any other amino acid.
  • preferred group of such active analogs does not include SEQ ID NO:31.
  • active analogs of colostrinin and its constituent peptides include polypeptides having structural similarity.
  • Structural similarity is generally determined by aligning the residues of the two amino acid sequences to optimize the number of identical amino acids along the lengths of their sequences; gaps in either or both sequences are permitted in making the alignment in order to optimize the number of identical amino acids, although the amino acids in each sequence must nonetheless remain in their proper order.
  • two amino acid sequences are compared using the Blastp program, version 2.0.9, of the BLAST 2 search algorithm, available at ht ⁇ ://www.ncbi.nlm.n ⁇ .gov/gor%12.html.
  • an active analog of colostrinin or its constituent peptides has a structural similarity to colostrinin or one or more of its constituent peptides (preferably, one of SEQ ID NOs: 1-30) of at least about 70% identity, more preferably, at least about 80% identity, and most preferably, at least about 90% identity.
  • Colostrinin or any combination of its peptide components or active analogs thereof can be derived (preferably, isolated and purified) naturally such as by extraction from colostrum or can be synthetically constructed using known peptide polymerization techniques.
  • the peptides of the invention may be synthesized by the solid phase method using standard methods based on either t-butyloxycarbonyl (BOC) or 9-fluorenylmethoxy-carbonyl (FMOC) protecting groups. This methodology is described by G.B. Fields et al. in Synthetic Peptides: A User's Guide. W.M. Freeman & Company, New York, NY, pp. 77-183 (1992).
  • gene sequence encoding the colostrinin peptides or analogs thereof can be constructed by known techniques such as expression vectors or plasmids and transfected into suitable microorganisms that will express the DNA sequences thus preparing the peptide for later extraction from the medium in which the microorganism are grown.
  • U.S. Patent No. 5,595,887 describes methods of forming a variety of relatively small peptides through expression of a recombinant gene construct coding for a fusion protein which includes a binding protein and one or more copies of the desired target peptide. After expression, the fusion protein is isolated and cleaved using chemical and/or enzymatic methods to produce the desired target peptide.
  • the peptides used in the methods of the present invention may be employed in a monovalent state (i.e., free peptide or a single peptide fragment coupled to a carrier molecule).
  • the peptides may also be employed as conjugates having more than one (same or different) peptide fragment bound to a single carrier molecule.
  • the carrier may be a biological carrier molecule (e.g., a glycosaminoglycan, a proteoglycan, burnin or the like) or a synthetic polymer (e.g., a polyalkyleneglycol or a synthetic chromatography support).
  • a synthetic polymer e.g., a polyalkyleneglycol or a synthetic chromatography support
  • ovalbumin, human serum albumin, other proteins, polyethylene glycol, or the like are employed as the carrier.
  • Such modifications may increase the apparent affinity and/or change the stability of a peptide.
  • peptide/carrier molecule conjugates may be prepared by treating a mixture of peptides and carrier molecules with a coupling agent, such as a carbodiimide.
  • the coupling agent may activate a carboxyl group on either the peptide or the carrier molecule so that the carboxyl group can react with a nucleophile (e.g., an amino or hydroxyl group) on the other member of the peptide/carrier molecule, resulting in the covalent linkage of the peptide and the carrier molecule.
  • conjugates of a peptide coupled to ovalbumin may be prepared by dissolving equal amounts of lyophilized peptide and ovalbumin in a small volume of water.
  • l-ethyl-3-(3- dimethylammo-propyl)-carbodiimide hydrochloride (EDC; ten times the amount of peptide) is dissolved in a small amount of water.
  • EDC l-ethyl-3-(3- dimethylammo-propyl)-carbodiimide hydrochloride
  • the EDC solution was added to the peptide/ovalburnin mixture and allowed to react for a number of hours.
  • the mixture may then dialyzed (e.g., into phosphate buffered saline) to obtain a purified solution of peptide/ovalburnin conjugate.
  • Peptide/carrier molecule conjugates prepared by this method typically contain about 4 to 5 peptides per ovalbumin molecule.
  • the present invention also provides a composition that includes one or more active agents (i.e., colostrinin, at least one constituent peptide thereof, or active analog thereof) of the invention and one or more carriers, preferably a pharmaceutically acceptable carrier.
  • the methods of the invention include administering to, or applying to the skin of, a patient, preferably a mammal, and more preferably a human, a composition of the invention in an amount effective to produce the desired effect.
  • the active agents of the present invention are formulated for enteral administration (oral, rectal, etc.) or parenteral adrninistration (injection, internal pump, etc.).
  • the administration can be via direct injection into tissue, interarterial injection, intervenous injection, or other internal adrninistration procedures, such as through the use of an implanted pump, or via contacting the composition with a mucus membrane in a carrier designed to facilitate transmission of the composition across the mucus membrane such as a suppository, eye drops, inhaler, or other similar administration method or via oral adrninistration in the form of a syrup, a liquid, a pill, capsule, gel coated tablet, or other similar oral administration method.
  • the active agents can be inco ⁇ orated into an adhesive plaster, a patch, a gum, and the like, or it can be encapsulated or inco ⁇ orated into a bio-erodible matrix for controlled release.
  • the carriers for internal administration can be any carriers commonly used to facilitate the internal administration of compositions such as plasma, sterile saline solution, IV solutions or the like.
  • Carriers for administration through mucus membranes can be any well-known in the art.
  • Carriers for administration oral can be any carrier well-known in the art.
  • the formulations may be conveniently presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active agent into association with a carrier which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active agent into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into the desired formulations.
  • Formulations suitable for parenteral aclministration conveniently include a sterile aqueous preparation of the active agent, or dispersions of sterile powders of the active agent, which are preferably isotonic with the blood of the recipient.
  • Isotonic agents that can be included in the liquid preparation include sugars, buffers, and sodium chloride.
  • Solutions of the active agent can be prepared in water, optionally mixed with a nontoxic surfactant.
  • Dispersions of the active agent can be prepared in water, ethanol, a polyol (such as glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, glycerol esters, and mixtures thereof.
  • the ultimate dosage form is sterile, fluid, and stable under the conditions of manufacture and storage.
  • the necessary fluidity can be achieved, for example, by using liposomes, by employing the appropriate particle size in the case of dispersions, or by using surfactants.
  • Sterilization of a liquid preparation can be achieved by any convenient method that preserves the bioactivity of the active agent, preferably by filter sterilization. Preferred methods for preparing powders include vacuum drying and freeze drying of the sterile injectible solutions. Subsequent microbial contamination can be prevented using various antimicrobial agents, for example, antibacterial, antiviral and antifungal agents including parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. Abso ⁇ tion of the active agents over a prolonged period can be achieved by including agents for delaying, for example, aluminum monostearate and gelatin.
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as tablets, troches, capsules, lozenges, wafers, or cachets, each containing a predetermined amount of the active agent as a powder or granules, as liposomes containing the active agent, or as a solution or suspension in an aqueous liquor or non-aqueous liquid such as a syrup, an elixir, an emulsion, or a draught.
  • the amount of active agent is such that the dosage level will be effective to produce the desired result in the subject.
  • Nasal spray formulations include purified aqueous solutions of the active agent with preservative agents and isotonic agents.
  • Such formulations are preferably adjusted to a pH and isotonic state compatible with the nasal mucous membranes.
  • Formulations for rectal or vaginal administration may be presented as a suppository with a suitable carrier such as cocoa butter, or hydrogenated fats or hydrogenated fatty carboxylic acids.
  • Ophthalmic formulations are prepared by a similar method to the nasal spray, except that the pH and isotonic factors are preferably adjusted to match that of the eye.
  • Topical formulations include the active agent dissolved or suspended in one or more media such as mineral oil, DMSO, polyhydroxy alcohols, or other bases used for topical pharmaceutical formulations.
  • Useful dosages of the active agents can be determined by comparing their in vitro activity and the in vivo activity in animal models. Methods for extrapolation of effective dosages in mice, and other animals, to humans are known in the art; for example, see U.S. Patent No. 4,938,949.
  • the tablets, troches, pills, capsules, and the like may also contain one or more of the following: a binder such as gum tragacanth, acacia, corn starch or gelatin; an excipient such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; a sweetening agent such as sucrose, fructose, lactose or aspartame; and a natural or artificial flavoring agent.
  • a binder such as gum tragacanth, acacia, corn starch or gelatin
  • an excipient such as dicalcium phosphate
  • a disintegrating agent such as corn starch, potato starch, alginic acid and the like
  • a lubricant such as magnesium stearate
  • a sweetening agent such as sucrose, fructose, lactose or aspartame
  • a natural or artificial flavoring agent such
  • Various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form.
  • tablets, pills, or capsules may be coated with gelatin, wax, shellac, or sugar and the like.
  • a syrup or elixir may contain one or more of a sweetening agent, a preservative such as methyl- or propylparaben, an agent to retard crystallization of the sugar, an agent to increase the solubility of any other ingredient, such as a polyhydric alcohol, for example glycerol or sorbitol, a dye, and flavoring agent.
  • the material used in preparing any unit dosage form is substantially nontoxic in the amounts employed.
  • the active agent may be inco ⁇ orated into sustained-release preparations and devices.
  • fluorenylmethyloxycarbonyl) amino acid 2.1 ml of 0.45 M HBTU/ ⁇ OBT (1 mmol) (2-(lH-benzotriazol-l -yl)-l , 1 ,3,3-tetramethyluronium hexafluorophosphate/N-hydroxybenzotriazole-H 2 O) 348 ⁇ l of DIEA (2 mmol) ( ⁇ isopropylethylamine); and b. Add the solution to the resin and shake for a minimum of 30 minutes.
  • the peptide was cleaved from the resin with 5% H 2 O, 5% phenol, 3% Thionisole, 3% EDT (ethanedithiol), 3% triisopropylsilane and 81 % TFA for 2 hours.
  • the molecular weight of the synthesised peptides was checked by Matrix-Assisted Laser Deso ⁇ tion Time-of-Flight Mass Spectroscopy (LDMS), and the purity was checked by HPLC using a C- 18, 300 Angstrom, 5 ⁇ m column.
  • Induction of Bood Cell Proliferation The quantity of peripheral blood leukocyte (PBL) stimulation was determined by measuring the amount of ⁇ H- thymidine (1.0 to 2.0 ⁇ C mymidine/culture) inco ⁇ orated into triplicate cultures (4 x 10- PBLs/culture) stimulated with colostrinin and its constituent peptides (CCP) for 72 hours. ⁇ H-thymidine was then added and allowed to inco ⁇ orate for 24 hours.
  • Staphylococcal enterotoxin A (SEA, also referred to as "super antigen"), a specific T cell mitogen, was used as a positive control and for comparative pu ⁇ oses.
  • Colostrum and low and high iron containing baby formulas diluted 1 :5 and 1:10 were also used in some experiments to determine the relative stimulatory activity of these products.
  • Radioactivity was measured in a Matrix 9600 Direct Beta Counter. Six replicas of medium treated cultures were used to determine the mean background inco ⁇ orated counts. The data is expressed as the mean ⁇ H-thymidine counts per minute (CPM) above background. Results of one out of a total of six experiments are shown below in Table 1.
  • colostrinin and its constituent peptides are excellent inducers of PBL proliferation. Active concentrations ranged from 100 ⁇ g/ml to 0.1 ⁇ g/ml. Nine peptides and colostrinin and colostrum were tested. Certain peptides appeared to have greater activity than others with the maximum increase in proliferative activity being roughly 10 times above background. It appears that with many of the peptides, the active range of proliferation induction was present since concentrations as low as 0.1 ⁇ g/ml still had potent activity. Some of the peptides had more activity than colostrinin alone. Another interesting finding is that colostrum appears to have roughly an equivalent amount of activity as colostrinin.
  • SEA has the greatest activity and this is also not unexpected due to its classification as a super antigen.
  • PBL proliferation is an important part of the immune response both for generating antigen reactive cells and induction of numerous modulating cytokines. In the newborn these processes are essential as a building block for development of an optimal immune response and provide a protective host defense barrier against diseases associated with the neonatal gut.
  • Mitogenic Activity CPM above control as determined by 24-hour 3 H- thymidine inco ⁇ oration.
  • Cytokine concentrations were also determined from cells following 72 hours of incubation with concentrations of colistrinin and its constituent peptides (CCP) ranging from 100 to 0.1 ⁇ g/ml, and colostrum and high- or low-iron baby formula (Enfamil) at various dilutions. Supernatant fluids were then subjected to enzyme-linked immunosorbent assay (ELISA) for the following commercially available cytokines: interferon-gamma (IFN- ⁇ ), tumor necrosis factor alpha (TNF- ⁇ ), interleukin (TL)-4, EL-6, IL-10, and D -12.
  • IFN- ⁇ interferon-gamma
  • TNF- ⁇ tumor necrosis factor alpha
  • TL interleukin
  • IL-10 interleukin
  • Table 2 represents the results of approximately 250 single assays. More specifically, in these studies it was found that many of the peptides including colostrinin induced EFN- ⁇ and that the data corresponds with 3 H-thymidine inco ⁇ oration (Tables 1 and 3). Interestingly the maximum cytokine inducing activity of many of the peptides was not diluted out until the 1.0 or 0.1 ⁇ g/ml concentrations of peptide were used (Shaded numbers in Table 2), or in the case of IFN- ⁇ and TNF- ⁇ induction by SEQ ID NO:31 and SEQ ID NO: 1 , 0.1 ⁇ g/ml rather than higher concentrations.
  • the complexed peptides making up colostrinin and colostrum may be more stable and/or combinations of peptides in colostrinin and colostrum may be more potent. Additional factors that may account for the variations of the peptides in these studies include: 1) natural variations in the immune state of the individuals donating the leukocytes, 2) the possibility that aggregation occurred in samples stored in PBS, thus reducing in effective number of molecules able to react, and 3) the possibility that the individual peptides may be subject to oxidative damage or some other inactivating process.
  • Cytokines induced in human leukocyte cultures stimulated with CCP, colostrum or commercial milk formulas.
  • Example 3 SEQ ID NO: 1 100 6 110 0 10 4 ND ND
  • SEQ ID NO:2 100 0 258.6 0 10 0 551.3 0 1- 0 1205 0 0.1 0 325 0
  • Colostrum 10 602.2 0
  • the relative abilities of the various peptides to induce cytokines are shown in Table 3.
  • the peptides were ranked according to their abilities to induce the indicated cytokine by first comparing the raw numbers at the 0.1 ⁇ g/ml concentration followed by 1.0 ⁇ g/ml concentrations and then higher concentrations, i.e., 10 and 100 ⁇ g/ml. It can be noted that SEQ ID NOs:l, 8, 3, 2, and 31 were the best overall inducers in almost all cytokine and blood cell proliferation experiments. Peptides SEQ ED NOs:7, 4, and 5 were generally less effective as inducers.
  • SEQ ID NOs: 18, 19, 20, and 22 were compared to the initially tested SEQ ID NOs:l-8 and 31, the latter were better inducers.
  • SEQ ID NO:22 was generally the best inducer of those peptides having a ⁇ -casein precursor. It was also found that Enfamil low iron baby formula induced higher levels of cytokines than the Enfamil high iron formula. Table 3. Relative abilities of the various peptides to induce cytokines and proliferation

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Abstract

L'invention concerne l'utilisation de la colostrinine, d'un peptide constitutif et/ou d'un analogue de celle-ci comme régulateur immunologique et comme régulateur de globules sanguins chez des animaux, notamment chez l'être humain.
PCT/US2000/022775 2000-08-17 2000-08-17 Utilisation de la colostrinine, de ses peptides constitutifs et de ses analogues pour activer des cytokines WO2002013849A1 (fr)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6903068B1 (en) 1999-08-17 2005-06-07 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof for inducing cytokines
US6939847B2 (en) 1999-08-17 2005-09-06 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof, as oxidative stress regulators
US7119064B2 (en) 1999-08-17 2006-10-10 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules
EP1894943A3 (fr) * 2003-03-11 2008-05-28 ReGen Therapeutics Plc Purification de peptides à partir du colostrum

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998014473A1 (fr) * 1996-10-03 1998-04-09 Ludwick Hirszfeld Institute Of Immunology And Experimental Therapy Polish Academy Of Sciences Colostrinine et utilisations de celle-ci
WO2000075173A2 (fr) * 1999-06-02 2000-12-14 Regen Therapeutics Plc Peptides
WO2001011937A2 (fr) * 1999-08-17 2001-02-22 The University Of Texas System Utilisation de la colostrinine, de ses peptides constitutifs et de ses analogues pour activer des cytokines

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998014473A1 (fr) * 1996-10-03 1998-04-09 Ludwick Hirszfeld Institute Of Immunology And Experimental Therapy Polish Academy Of Sciences Colostrinine et utilisations de celle-ci
WO2000075173A2 (fr) * 1999-06-02 2000-12-14 Regen Therapeutics Plc Peptides
WO2001011937A2 (fr) * 1999-08-17 2001-02-22 The University Of Texas System Utilisation de la colostrinine, de ses peptides constitutifs et de ses analogues pour activer des cytokines

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
INGLOT A D ET AL: "COLOSTRININE: A PROLINE-RICH POLYPEPTIDE FROM OVINE COLOSTRUM IS A MODEST CYTOKINE INDUCET IN HUMAN LEUKOCYTES", ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS,PL,POLISH ACADEMY OF SCIENCES, WROCLAW, vol. 44, no. 4, 1 August 1996 (1996-08-01), pages 215 - 224, XP002055880, ISSN: 0004-069X *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6903068B1 (en) 1999-08-17 2005-06-07 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof for inducing cytokines
US6939847B2 (en) 1999-08-17 2005-09-06 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof, as oxidative stress regulators
US7119064B2 (en) 1999-08-17 2006-10-10 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules
EP1894943A3 (fr) * 2003-03-11 2008-05-28 ReGen Therapeutics Plc Purification de peptides à partir du colostrum

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