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WO2002033052A3 - Procede de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat - Google Patents

Procede de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat Download PDF

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Publication number
WO2002033052A3
WO2002033052A3 PCT/DE2001/003959 DE0103959W WO0233052A3 WO 2002033052 A3 WO2002033052 A3 WO 2002033052A3 DE 0103959 W DE0103959 W DE 0103959W WO 0233052 A3 WO0233052 A3 WO 0233052A3
Authority
WO
WIPO (PCT)
Prior art keywords
support
vitro
cell cultures
cell
tissue
Prior art date
Application number
PCT/DE2001/003959
Other languages
German (de)
English (en)
Other versions
WO2002033052A2 (fr
Inventor
Ralf Sodian
Thees Lembke
Original Assignee
Deutsches Herzzentrum Berlin
Ralf Sodian
Thees Lembke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Herzzentrum Berlin, Ralf Sodian, Thees Lembke filed Critical Deutsches Herzzentrum Berlin
Priority to AU2002221526A priority Critical patent/AU2002221526A1/en
Priority to EP01987795A priority patent/EP1326962A2/fr
Publication of WO2002033052A2 publication Critical patent/WO2002033052A2/fr
Publication of WO2002033052A3 publication Critical patent/WO2002033052A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Mechanical Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat. Ce procédé consiste à soumettre une construction cellules-tissu, constituée du substrat et des cultures cellulaires, à une sollicitation mécanique fluctuante qui associe notamment un écoulement pulsé d'un milieu de culture cellulaire et un mouvement pulsé de la construction cellules-tissu.
PCT/DE2001/003959 2000-10-17 2001-10-17 Procede de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat WO2002033052A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002221526A AU2002221526A1 (en) 2000-10-17 2001-10-17 Method for producing vital, biological substitute tissue in vitro using cell cultures and a support
EP01987795A EP1326962A2 (fr) 2000-10-17 2001-10-17 Procede de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10053014A DE10053014A1 (de) 2000-10-17 2000-10-17 Verfahren zur In-vitro-Herstellung von vitalem, biologischem Ersatzgewebe unter Verwendung von Zellkulturen und einem Trägermaterial
DE10053014.1 2000-10-17

Publications (2)

Publication Number Publication Date
WO2002033052A2 WO2002033052A2 (fr) 2002-04-25
WO2002033052A3 true WO2002033052A3 (fr) 2002-11-28

Family

ID=7661079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003959 WO2002033052A2 (fr) 2000-10-17 2001-10-17 Procede de production in vitro de tissu de remplacement biologique vital au moyen de cultures cellulaires et d'un substrat

Country Status (4)

Country Link
EP (1) EP1326962A2 (fr)
AU (1) AU2002221526A1 (fr)
DE (1) DE10053014A1 (fr)
WO (1) WO2002033052A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8399243B2 (en) 2005-02-17 2013-03-19 Universitaet Zuerich Method of manufacturing a tissue-engineered prosthesis

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10249903B4 (de) * 2002-10-22 2007-10-11 Cytonet Gmbh & Co. Kg Mechanischer Bioreaktor
DE10322024A1 (de) * 2003-05-16 2004-12-02 Symetis Ag Bioreaktor zum Herstellen einer Gewebeprothese, insbesondere Herzklappe
DE102004001225B8 (de) * 2004-01-05 2006-07-27 fzmb Forschungszentrum für Medizintechnik und Biotechnologie e.V. Verfahren zur Herstellung dreidimensionaler trägerfreier Gewebestrukturen und nach diesem Verfahren hergestellte Gewebestrukturen
DE102008050424B4 (de) 2008-10-08 2010-11-25 Universität Leipzig Verfahren und Vorrichtung zur homogenen Verteilung einer zellulären Suspension in porösem Trägermaterial für die Herstellung von vitalem biologischem Ersatzgewebe
DE102009039535B4 (de) * 2009-09-01 2015-08-20 Corlife Ohg Behälter, Vorrichtung und Verfahren zur Behandlung von Gewebe unter Reinraumbedingungen
EP2399986A1 (fr) 2010-06-22 2011-12-28 Ludwig-Maximilians-Universität München Bioréacteur et procédé de création et/ou de conditionnement de tissus biologiques
EP2468846A1 (fr) 2010-12-22 2012-06-27 Ludwig-Maximilians-Universität München Procédé pour examiner la croissance tissulaire et le conditionnement de cellules sur un support et bioréacteur à perfusion
EP2500410A1 (fr) 2011-03-18 2012-09-19 Ludwig-Maximilians-Universität München Bioréacteur avec supports de stimulation mécanique et électrique
CZ2012376A3 (cs) 2012-06-05 2013-12-18 Institut Klinické A Experimentální Medicíny Způsob výroby perikardiální náhrady srdeční chlopně, perikardiální náhrada srdeční chlopně vyrobená tímto způsobem, zařízení pro kondicioning a modifikaci tkáně autologního perikardu pro perikardiální náhradu srdeční chlopně
JP7270657B2 (ja) * 2019-02-04 2023-05-10 富士フイルム株式会社 細胞培養装置、細胞培養方法、及び生産物の製造方法
IT202000021847A1 (it) * 2020-09-16 2022-03-16 React4Life S R L Dispositivo per l’esecuzione di esperimenti in vitro su materiale biologico
CN113249211A (zh) * 2021-05-26 2021-08-13 庆开雄 一种可改变箱内压力的细胞培养箱及其压力调节方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153136A (en) * 1988-07-22 1992-10-06 Vandenburgh Herman H Apparatus for growing tissue specimens in vitro
US5348879A (en) * 1990-08-24 1994-09-20 The General Hospital Corporation Cell stretching method
WO1997049799A1 (fr) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Dispositif et procede de sterilisation, d'ensemencement, de culture, de stockage, de transport et de controle de greffes de tissus vasculaires, d'origine synthetique ou naturelle

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153136A (en) * 1988-07-22 1992-10-06 Vandenburgh Herman H Apparatus for growing tissue specimens in vitro
US5348879A (en) * 1990-08-24 1994-09-20 The General Hospital Corporation Cell stretching method
WO1997049799A1 (fr) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Dispositif et procede de sterilisation, d'ensemencement, de culture, de stockage, de transport et de controle de greffes de tissus vasculaires, d'origine synthetique ou naturelle

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HOERSTRUP SIMON P ET AL: "New pulsatile bioreactor for in vitro formation of tissue engineered heart valves.", TISSUE ENGINEERING, vol. 6, no. 1, February 2000 (2000-02-01), pages 75 - 79, XP001040483, ISSN: 1076-3279 *
NIKLASON L E ET AL: "Functional arteries grown in vitro.", SCIENCE (WASHINGTON D C), vol. 284, no. 5413, 16 April 1999 (1999-04-16), pages 489 - 493, XP002200891, ISSN: 0036-8075 *
SODIAN R ET AL: "NEW PULSATILE BIORECTOR FOR FABRICATION OF TISSUE-ENGINEERED PATCHES", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, WILEY, NEW YORK, NY, US, vol. 58, no. 4, 2001, pages 401 - 405, XP001037742, ISSN: 0021-9304 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8399243B2 (en) 2005-02-17 2013-03-19 Universitaet Zuerich Method of manufacturing a tissue-engineered prosthesis

Also Published As

Publication number Publication date
EP1326962A2 (fr) 2003-07-16
WO2002033052A2 (fr) 2002-04-25
DE10053014A1 (de) 2002-04-18
AU2002221526A1 (en) 2002-04-29

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