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WO2003040679A3 - Liaison reversible d'un fluorophore sur une surface pour detecter des phenomenes d'association ligat-ligand par extinction de fluorescence - Google Patents

Liaison reversible d'un fluorophore sur une surface pour detecter des phenomenes d'association ligat-ligand par extinction de fluorescence Download PDF

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Publication number
WO2003040679A3
WO2003040679A3 PCT/DE2002/004147 DE0204147W WO03040679A3 WO 2003040679 A3 WO2003040679 A3 WO 2003040679A3 DE 0204147 W DE0204147 W DE 0204147W WO 03040679 A3 WO03040679 A3 WO 03040679A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorophore
detecting
ligate
fluorescence quenching
modified
Prior art date
Application number
PCT/DE2002/004147
Other languages
German (de)
English (en)
Other versions
WO2003040679A2 (fr
Inventor
Gerhard Hartwich
Thomas Kratzmueller
Herbert Wieder
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Thomas Kratzmueller
Herbert Wieder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Friz Biochem Gmbh, Gerhard Hartwich, Thomas Kratzmueller, Herbert Wieder filed Critical Friz Biochem Gmbh
Priority to AU2002363452A priority Critical patent/AU2002363452A1/en
Publication of WO2003040679A2 publication Critical patent/WO2003040679A2/fr
Publication of WO2003040679A3 publication Critical patent/WO2003040679A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé pour détecter des phénomènes d'association ligat-ligand par extinction de fluorescence, ce procédé consistant d'abord à préparer une surface modifiée. La modification de surface consiste à établir une liaison d'au moins une catégorie de ligats 201 modifiés, lesquels sont modifiés par liaison avec au moins un type de fluorophore 102, qui établit une liaison réversible avec la surface modifiée. Les autres opérations du procédé de la présente invention sont les suivantes : préparation d'un échantillon de ligands, mise en contact de cet échantillon avec la surface modifiée, détection de la fluorescence du fluorophore et comparaison de l'intensité de la fluorescence détectée avec des valeurs de référence.
PCT/DE2002/004147 2001-11-09 2002-11-08 Liaison reversible d'un fluorophore sur une surface pour detecter des phenomenes d'association ligat-ligand par extinction de fluorescence WO2003040679A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002363452A AU2002363452A1 (en) 2001-11-09 2002-11-08 Reversibly binding a fluorophore to a surface for the purpose of detecting ligate/ligand association events by fluorescence quenching

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2001155053 DE10155053B4 (de) 2001-11-09 2001-11-09 Reversible Bindung eines Fluorophors an eine Oberfläche zur Detektion von Ligat-Ligand-Assoziationsereignissen durch Fluoreszenz-Quenchen
DE10155053.7 2001-11-09

Publications (2)

Publication Number Publication Date
WO2003040679A2 WO2003040679A2 (fr) 2003-05-15
WO2003040679A3 true WO2003040679A3 (fr) 2004-05-06

Family

ID=7705170

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/004147 WO2003040679A2 (fr) 2001-11-09 2002-11-08 Liaison reversible d'un fluorophore sur une surface pour detecter des phenomenes d'association ligat-ligand par extinction de fluorescence

Country Status (3)

Country Link
AU (1) AU2002363452A1 (fr)
DE (1) DE10155053B4 (fr)
WO (1) WO2003040679A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0419325D0 (en) * 2004-09-01 2004-09-29 Perkinelmer Ltd A method of analysing a sample including fluorescent labels and apparatus therefor
CN100396790C (zh) * 2004-09-17 2008-06-25 北京大学 溶液识别、表面寻址蛋白质芯片及其制备和检测方法
DE102016000865A1 (de) * 2016-01-28 2017-08-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Funktionalisierte metallische Nanopartikel

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000077260A1 (fr) * 1999-06-15 2000-12-21 Genomic Profiling Systems, Inc. Etablissement de profil genomique: procede rapide permettant d'analyser un echantillon biologique complexe afin de deceler la presence de nombreux types d'organismes
WO2002018951A2 (fr) * 2000-08-29 2002-03-07 The Rockefeller University Procedes mettant en application une extinction de fluorescence par des surfaces metalliques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19811730A1 (de) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Identifizieren einer Markierung
US6645733B1 (en) * 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
AU5601800A (en) 1999-08-09 2001-03-05 Motorola, Inc. Adaptive delay lock loop tracking
EP1242626B1 (fr) * 1999-12-21 2009-06-10 Ortho-Clinical Diagnostics, Inc. Detection d'acides nucleiques
US6265170B1 (en) * 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000077260A1 (fr) * 1999-06-15 2000-12-21 Genomic Profiling Systems, Inc. Etablissement de profil genomique: procede rapide permettant d'analyser un echantillon biologique complexe afin de deceler la presence de nombreux types d'organismes
WO2002018951A2 (fr) * 2000-08-29 2002-03-07 The Rockefeller University Procedes mettant en application une extinction de fluorescence par des surfaces metalliques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DUBERTRET B ET AL: "Single-mismatch detection using gold-quenched fluorescent oligonucleotides", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 19, no. 4, April 2001 (2001-04-01), pages 365 - 370, XP002224627, ISSN: 1087-0156 *
PEREZ-LUNA V H ET AL: "Fluorescence biosensing strategy based on energy transfer between fluorescently labeled receptors and a metallic surface", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 17, no. 1-2, January 2002 (2002-01-01), pages 71 - 78, XP002256222, ISSN: 0956-5663 *

Also Published As

Publication number Publication date
WO2003040679A2 (fr) 2003-05-15
AU2002363452A1 (en) 2003-05-19
DE10155053A1 (de) 2003-08-14
DE10155053B4 (de) 2005-11-24

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