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WO2003048361A3 - P55pik - Google Patents

P55pik Download PDF

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Publication number
WO2003048361A3
WO2003048361A3 PCT/GB2002/005547 GB0205547W WO03048361A3 WO 2003048361 A3 WO2003048361 A3 WO 2003048361A3 GB 0205547 W GB0205547 W GB 0205547W WO 03048361 A3 WO03048361 A3 WO 03048361A3
Authority
WO
WIPO (PCT)
Prior art keywords
p55pik
polypeptide
iii
nucleic acid
set out
Prior art date
Application number
PCT/GB2002/005547
Other languages
French (fr)
Other versions
WO2003048361A2 (en
Inventor
Ian Hayes
Thomas Cotter
Finbarr Murphy
Liam Seery
Original Assignee
Eirx Therapeutics Ltd
Ian Hayes
Thomas Cotter
Finbarr Murphy
Liam Seery
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0129377A external-priority patent/GB0129377D0/en
Priority claimed from GB0200831A external-priority patent/GB0200831D0/en
Application filed by Eirx Therapeutics Ltd, Ian Hayes, Thomas Cotter, Finbarr Murphy, Liam Seery filed Critical Eirx Therapeutics Ltd
Priority to AU2002347368A priority Critical patent/AU2002347368A1/en
Publication of WO2003048361A2 publication Critical patent/WO2003048361A2/en
Publication of WO2003048361A3 publication Critical patent/WO2003048361A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01137—Phosphatidylinositol 3-kinase (2.7.1.137)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention provides a method for detecting apoptosis in a cell comprising detecting an alteration in any one of: i) a p55PIK polypeptide having an amino acid sequence as set out in SEQ ID NO:1; ii) a polypeptide having at least 80 % homology with i); iii) a nucleic acid encoding a polypeptide having the sequence set out in i) or ii); iv) a nucleic acid which hybridises under stringent conditions to the sequence set out in iii); or v) the complement of iii) or iv). The invention accordingly provides a method of modulating apoptosis by modulating p55PIK gene expression and a method for identifying genes associated with p55PIK gene expression and thus identifying other genes associated with apoptosis. The invention also provides a novel nucleic acid sequence encoding the promoter region for p55PIK.
PCT/GB2002/005547 2001-12-07 2002-12-06 P55pik WO2003048361A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002347368A AU2002347368A1 (en) 2001-12-07 2002-12-06 P55pik

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0129377.8 2001-12-07
GB0129377A GB0129377D0 (en) 2001-12-07 2001-12-07 p55PIK
GB0200831.6 2002-01-15
GB0200831A GB0200831D0 (en) 2002-01-15 2002-01-15 p55PIK

Publications (2)

Publication Number Publication Date
WO2003048361A2 WO2003048361A2 (en) 2003-06-12
WO2003048361A3 true WO2003048361A3 (en) 2004-03-18

Family

ID=26246851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/005547 WO2003048361A2 (en) 2001-12-07 2002-12-06 P55pik

Country Status (2)

Country Link
AU (1) AU2002347368A1 (en)
WO (1) WO2003048361A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100090A (en) * 1999-06-25 2000-08-08 Isis Pharmaceuticals Inc. Antisense inhibition of PI3K p85 expression
US6165790A (en) * 1999-11-03 2000-12-26 Isis Pharmaceuticals Inc. Antisense modulation of PI3 kinase p55 gamma expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100090A (en) * 1999-06-25 2000-08-08 Isis Pharmaceuticals Inc. Antisense inhibition of PI3K p85 expression
US6165790A (en) * 1999-11-03 2000-12-26 Isis Pharmaceuticals Inc. Antisense modulation of PI3 kinase p55 gamma expression

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BERNABÉ J C ET AL: "Sodium nitroprusside-induced mitochondrial apoptotic events in insulin-secreting RINm5F cells are associated with MAP kinases activation.", EXPERIMENTAL CELL RESEARCH. UNITED STATES 1 OCT 2001, vol. 269, no. 2, 1 October 2001 (2001-10-01), pages 222 - 229, XP002256953, ISSN: 0014-4827 *
DATABASE NCBI SNP [online] 28 June 2001 (2001-06-28), XP002256956, accession no. WWW.NCBI.NLM.NIH.GOV/SNP Database accession no. rs785468, ss3177819 *
DEY B R ET AL: "CLONING OF HUMAN P55GAMMA, A REGULATORY SUBUNIT OF PHOSPHATIDYLINOSITOL 3-KINASE, BY YEAST TWO-HYBRID LIBRARY SCREEN WITH THE INSULIN-LIKE GROWTH FACTOR-I RECEPTOR", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 209, 1998, pages 175 - 183, XP002938192, ISSN: 0378-1119 *
INUKAI K ET AL: "The N-terminal 34 residues of the 55 kDa regulatory subunits of phosphoinositide 3-kinase interact with tubulin.", THE BIOCHEMICAL JOURNAL. ENGLAND 1 MAR 2000, vol. 346 Pt 2, 1 March 2000 (2000-03-01), pages 483 - 489, XP002256955, ISSN: 0264-6021 *
JOST M ET AL: "Epidermal growth factor receptor-dependent control of keratinocyte survival and Bcl-xL expression through a MEK-dependent pathway.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 2 MAR 2001, vol. 276, no. 9, 2 March 2001 (2001-03-02), pages 6320 - 6326, XP002256952, ISSN: 0021-9258 *
SHIN B C ET AL: "Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. UNITED STATES 19 MAY 1998, vol. 246, no. 2, 19 May 1998 (1998-05-19), pages 313 - 319, XP002256954, ISSN: 0006-291X *

Also Published As

Publication number Publication date
WO2003048361A2 (en) 2003-06-12
AU2002347368A8 (en) 2003-06-17
AU2002347368A1 (en) 2003-06-17

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